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The map label for this gene is FolD

Identifier: 20807742

GI number: 20807742

Start: 1283912

End: 1284754

Strand: Direct

Name: FolD

Synonym: TTE1293

Alternate gene names: 20807742

Gene position: 1283912-1284754 (Clockwise)

Preceding gene: 20807741

Following gene: 20807743

Centisome position: 47.74

GC content: 39.62

Gene sequence:

>843_bases
GTGAAGCGAATGATAATTGATGGAAAAGAAATTTCTAATAGAATAAAAGAAGAAGTTAAAAGGGAAATACAGGAATTTGG
TTACAAACCTAGATTGGCAATATTGATGGCAGGAGATGACGAATCCTCAAAGGTCTATGCCAACTCCAAAGTGAAGGCCT
GTGAGAGCGTAGGGATAGAAGCAAAAGTGTACTATTTTTCAGAAAAAGAGGAAGATAAATTTTTTGATACTTTAGAAAAG
TTAAATGAAGATAAAGACACCCACGGGATAATGATAGAGATGCCTCTTCCAAAGGGCTTTGATGTAGACAAAGTTTACGA
TACGATAAATCCGTACAAAGATGTTGATTGCATATCAAATTACAATATGGGCAGGCTTTTTGCTGGAAAACCTCTTTTTG
TTCCCTGTACGCCGAAGGCTATTATCCATATATTGGAAAATACAGGGGTTGACTTGGAAGGAAAACACGCCGTGGTGATA
GGAAGGAGCAATATTTTAGGAAAGCCAGTTGCCAAGCTTTTGCTGGACAAAAACTGCACTGTGACAGTATGCCACTCTAA
GACAAAGGATTTAGCTTATCATACGAAACAGGCAGATGTTTTGGTGGTAGCAGCAGGAAAGATGAATCTGGTTAGAGGGG
ACATGGTCAAAGAGGGAGTAGTTTTAATAGATGCAGGGATAAACGTACATGAAGGTAAGATATACGGCGATGCCGACTTT
GAGTCAATTAAGGACAAGGCTTCTTATGTAACCCCTGTGCCGGGAGGAGTTGGACCTGTAACTACAGCCATGATTCTCAA
AAATACCCTGGAGGCTTTTAAATATGCAGTTAAAAGCCTTTAG

Upstream 100 bases:

>100_bases
GAAGCGGTAGAAATAGCAAAAAAATATAGCACTGACGATTCTCCCTCTTTCATAAACGGGCTTTTGGGAGCATTCGTCAG
GGAAAAAGGATTAGAGGAAG

Downstream 100 bases:

>100_bases
TGTTAGTGAAGTGACGGAATATATAAAGAAAGTTATGGACAACGACATAATATTGAGAAATGTGAGAGTGAGAGGGGAAA
TTTCTAATTTAAAATACCAC

Product: 5,10-methylene-tetrahydrofolate dehydrogenase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MKRMIIDGKEISNRIKEEVKREIQEFGYKPRLAILMAGDDESSKVYANSKVKACESVGIEAKVYYFSEKEEDKFFDTLEK
LNEDKDTHGIMIEMPLPKGFDVDKVYDTINPYKDVDCISNYNMGRLFAGKPLFVPCTPKAIIHILENTGVDLEGKHAVVI
GRSNILGKPVAKLLLDKNCTVTVCHSKTKDLAYHTKQADVLVVAAGKMNLVRGDMVKEGVVLIDAGINVHEGKIYGDADF
ESIKDKASYVTPVPGGVGPVTTAMILKNTLEAFKYAVKSL

Sequences:

>Translated_280_residues
MKRMIIDGKEISNRIKEEVKREIQEFGYKPRLAILMAGDDESSKVYANSKVKACESVGIEAKVYYFSEKEEDKFFDTLEK
LNEDKDTHGIMIEMPLPKGFDVDKVYDTINPYKDVDCISNYNMGRLFAGKPLFVPCTPKAIIHILENTGVDLEGKHAVVI
GRSNILGKPVAKLLLDKNCTVTVCHSKTKDLAYHTKQADVLVVAAGKMNLVRGDMVKEGVVLIDAGINVHEGKIYGDADF
ESIKDKASYVTPVPGGVGPVTTAMILKNTLEAFKYAVKSL
>Mature_280_residues
MKRMIIDGKEISNRIKEEVKREIQEFGYKPRLAILMAGDDESSKVYANSKVKACESVGIEAKVYYFSEKEEDKFFDTLEK
LNEDKDTHGIMIEMPLPKGFDVDKVYDTINPYKDVDCISNYNMGRLFAGKPLFVPCTPKAIIHILENTGVDLEGKHAVVI
GRSNILGKPVAKLLLDKNCTVTVCHSKTKDLAYHTKQADVLVVAAGKMNLVRGDMVKEGVVLIDAGINVHEGKIYGDADF
ESIKDKASYVTPVPGGVGPVTTAMILKNTLEAFKYAVKSL

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=287, Percent_Identity=40.0696864111498, Blast_Score=225, Evalue=4e-59,
Organism=Homo sapiens, GI222418558, Length=292, Percent_Identity=38.6986301369863, Blast_Score=203, Evalue=1e-52,
Organism=Homo sapiens, GI94721354, Length=296, Percent_Identity=36.1486486486487, Blast_Score=191, Evalue=8e-49,
Organism=Homo sapiens, GI36796743, Length=237, Percent_Identity=25.7383966244726, Blast_Score=85, Evalue=8e-17,
Organism=Escherichia coli, GI1786741, Length=278, Percent_Identity=44.2446043165468, Blast_Score=242, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI17568735, Length=287, Percent_Identity=38.3275261324042, Blast_Score=189, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6319558, Length=285, Percent_Identity=40, Blast_Score=218, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6321643, Length=297, Percent_Identity=39.0572390572391, Blast_Score=201, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6322933, Length=321, Percent_Identity=24.6105919003115, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24645718, Length=284, Percent_Identity=40.8450704225352, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI17137370, Length=284, Percent_Identity=40.8450704225352, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI62472483, Length=284, Percent_Identity=40.8450704225352, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI45551871, Length=284, Percent_Identity=40.8450704225352, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI17136816, Length=291, Percent_Identity=35.7388316151203, Blast_Score=186, Evalue=1e-47,
Organism=Drosophila melanogaster, GI17136818, Length=291, Percent_Identity=35.7388316151203, Blast_Score=186, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_THETN (Q8RAD0)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_622913.1
- HSSP:   P24186
- ProteinModelPortal:   Q8RAD0
- SMR:   Q8RAD0
- GeneID:   997645
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE1293
- NMPDR:   fig|273068.3.peg.1184
- HOGENOM:   HBG328751
- OMA:   IIHILEN
- ProtClustDB:   CLSK901151
- BioCyc:   TTEN273068:TTE1293-MONOMER
- BRENDA:   1.5.1.5
- BRENDA:   3.5.4.9
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30996; Mature: 30996

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2; PS00136 SUBTILASE_ASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRMIIDGKEISNRIKEEVKREIQEFGYKPRLAILMAGDDESSKVYANSKVKACESVGIE
CCCEEECCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEECCHHHHHHHCCCE
AKVYYFSEKEEDKFFDTLEKLNEDKDTHGIMIEMPLPKGFDVDKVYDTINPYKDVDCISN
EEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCCHHHCC
YNMGRLFAGKPLFVPCTPKAIIHILENTGVDLEGKHAVVIGRSNILGKPVAKLLLDKNCT
CCCCEEECCCEEEEECCHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCE
VTVCHSKTKDLAYHTKQADVLVVAAGKMNLVRGDMVKEGVVLIDAGINVHEGKIYGDADF
EEEECCCCCHHEEECCCCCEEEEECCCCEEEECCHHHCCEEEEECCCEEECCEEECCCCH
ESIKDKASYVTPVPGGVGPVTTAMILKNTLEAFKYAVKSL
HHHHHHCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKRMIIDGKEISNRIKEEVKREIQEFGYKPRLAILMAGDDESSKVYANSKVKACESVGIE
CCCEEECCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEECCHHHHHHHCCCE
AKVYYFSEKEEDKFFDTLEKLNEDKDTHGIMIEMPLPKGFDVDKVYDTINPYKDVDCISN
EEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCCHHHCC
YNMGRLFAGKPLFVPCTPKAIIHILENTGVDLEGKHAVVIGRSNILGKPVAKLLLDKNCT
CCCCEEECCCEEEEECCHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCE
VTVCHSKTKDLAYHTKQADVLVVAAGKMNLVRGDMVKEGVVLIDAGINVHEGKIYGDADF
EEEECCCCCHHEEECCCCCEEEEECCCCEEEECCHHHCCEEEEECCCEEECCEEECCCCH
ESIKDKASYVTPVPGGVGPVTTAMILKNTLEAFKYAVKSL
HHHHHHCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336