Click here to switch to the map view.

The map label for this gene is MurI3

Identifier: 20807679

GI number: 20807679

Start: 1225135

End: 1225950

Strand: Direct

Name: MurI3

Synonym: TTE1225

Alternate gene names: 20807679

Gene position: 1225135-1225950 (Clockwise)

Preceding gene: 20807678

Following gene: 20807681

Centisome position: 45.55

GC content: 31.5

Gene sequence:

>816_bases
TTGTGGGGGGATAAAATGAGATACTTTCCGATAGGAGTATTTGATTCGGGGGTTGGGGGACTTACAGTACTTAAACGCCT
TATGGAAGTATTGCCACAAGAAAATTATGTTTATTTTGGGGATACAAGAAGAGTTCCCTATGGAGATAGAAGCAAGGAAG
AAATAAAAGCATTTACAAAGCAAATCATGAATTTTTTGAAAAAGAAAAATGTAAAAATCTTTGTAATTGCTTGTAATACT
ATTAGTGCTGTATTTGAAAAGGAAGGAAAGGAAATTGTATTTAATGTTGTAGAGGCAGGAGTTAAGAGTGCGGTAGAAAC
TACTTGCAATAAAAGGATTGGTGTTATTGGGACAAGAAGAACTACAGAAGAGAGAATCTATTCAAATAAAATAAAAGAAA
TTAATAAAAGCATAGAAGTGTATGAAGTAGCTTGTCCTGAGCTAGTGCCTCTAATAGAAAAAGGTTTTTACAAAACCGAG
TCAGTTAAAAGAGTGGTTATAGAATGTGTAAAAAATTTAAAGGAAGAAAAGATTGATACCTTGGTTTTAGGTTGTACACA
TTATCCAATAGTTATAGACTACATAGAGGAAGCATTAATAGGAGAAAATGTAAGAATAGTAGATCCTGCCGAAAGGTTAG
CCTACGATGTAAAAGAATATATTACAAGAGTAAACATGATAAATCCTAATGGTAGTGGCCAAGTTCAATTTTACTTGAGT
AAAATGACTCAAAGTTTTATTGAGATAGCTAAAATTCTTCTTGAAGGAAAAATAAATAATTATAATGTTTCTATTGTGGA
TATAACCAAATATTAG

Upstream 100 bases:

>100_bases
ATAAAGGGTTCTTACAAGTATTTTTTACCATCTACTGTGTTGATTGTTGTGTTTTTAATGTTATATTTTTTGATTTTATA
ATAAAAACTGGAAATCGTAG

Downstream 100 bases:

>100_bases
AGAGGTGAGGCAAATGGAATTTAAGAAAAAATTGGGATTTTGGAGTGTTGTCCTTTTAGGTATTAATGGAATAATAGGAG
CATTCCTGTAATGAATAAAA

Product: glutamate racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MWGDKMRYFPIGVFDSGVGGLTVLKRLMEVLPQENYVYFGDTRRVPYGDRSKEEIKAFTKQIMNFLKKKNVKIFVIACNT
ISAVFEKEGKEIVFNVVEAGVKSAVETTCNKRIGVIGTRRTTEERIYSNKIKEINKSIEVYEVACPELVPLIEKGFYKTE
SVKRVVIECVKNLKEEKIDTLVLGCTHYPIVIDYIEEALIGENVRIVDPAERLAYDVKEYITRVNMINPNGSGQVQFYLS
KMTQSFIEIAKILLEGKINNYNVSIVDITKY

Sequences:

>Translated_271_residues
MWGDKMRYFPIGVFDSGVGGLTVLKRLMEVLPQENYVYFGDTRRVPYGDRSKEEIKAFTKQIMNFLKKKNVKIFVIACNT
ISAVFEKEGKEIVFNVVEAGVKSAVETTCNKRIGVIGTRRTTEERIYSNKIKEINKSIEVYEVACPELVPLIEKGFYKTE
SVKRVVIECVKNLKEEKIDTLVLGCTHYPIVIDYIEEALIGENVRIVDPAERLAYDVKEYITRVNMINPNGSGQVQFYLS
KMTQSFIEIAKILLEGKINNYNVSIVDITKY
>Mature_271_residues
MWGDKMRYFPIGVFDSGVGGLTVLKRLMEVLPQENYVYFGDTRRVPYGDRSKEEIKAFTKQIMNFLKKKNVKIFVIACNT
ISAVFEKEGKEIVFNVVEAGVKSAVETTCNKRIGVIGTRRTTEERIYSNKIKEINKSIEVYEVACPELVPLIEKGFYKTE
SVKRVVIECVKNLKEEKIDTLVLGCTHYPIVIDYIEEALIGENVRIVDPAERLAYDVKEYITRVNMINPNGSGQVQFYLS
KMTQSFIEIAKILLEGKINNYNVSIVDITKY

Specific function: Provides the (R)-glutamate required for cell wall biosynthesis

COG id: COG0796

COG function: function code M; Glutamate racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartate/glutamate racemases family

Homologues:

Organism=Escherichia coli, GI87082355, Length=211, Percent_Identity=29.8578199052133, Blast_Score=85, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURI3_THETN (Q8RAJ0)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_622850.1
- ProteinModelPortal:   Q8RAJ0
- SMR:   Q8RAJ0
- GeneID:   997300
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE1225
- NMPDR:   fig|273068.3.peg.1017
- HOGENOM:   HBG645102
- OMA:   CNTISAV
- ProtClustDB:   CLSK900684
- BioCyc:   TTEN273068:TTE1225-MONOMER
- BRENDA:   5.1.1.3
- HAMAP:   MF_00258
- InterPro:   IPR015942
- InterPro:   IPR001920
- InterPro:   IPR018187
- InterPro:   IPR004391
- Gene3D:   G3DSA:3.40.50.1860
- TIGRFAMs:   TIGR00067

Pfam domain/function: PF01177 Asp_Glu_race; SSF53681 Asp/Glu_race

EC number: =5.1.1.3

Molecular weight: Translated: 31012; Mature: 31012

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: PS00923 ASP_GLU_RACEMASE_1; PS00924 ASP_GLU_RACEMASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWGDKMRYFPIGVFDSGVGGLTVLKRLMEVLPQENYVYFGDTRRVPYGDRSKEEIKAFTK
CCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHH
QIMNFLKKKNVKIFVIACNTISAVFEKEGKEIVFNVVEAGVKSAVETTCNKRIGVIGTRR
HHHHHHHHCCCEEEEEECCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCEECCC
TTEERIYSNKIKEINKSIEVYEVACPELVPLIEKGFYKTESVKRVVIECVKNLKEEKIDT
CHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHE
LVLGCTHYPIVIDYIEEALIGENVRIVDPAERLAYDVKEYITRVNMINPNGSGQVQFYLS
EEEECCCCHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHEEECCCCCCEEEHHHH
KMTQSFIEIAKILLEGKINNYNVSIVDITKY
HHHHHHHHHHHHHHHCCCCCEEEEEEEEECC
>Mature Secondary Structure
MWGDKMRYFPIGVFDSGVGGLTVLKRLMEVLPQENYVYFGDTRRVPYGDRSKEEIKAFTK
CCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHH
QIMNFLKKKNVKIFVIACNTISAVFEKEGKEIVFNVVEAGVKSAVETTCNKRIGVIGTRR
HHHHHHHHCCCEEEEEECCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCEECCC
TTEERIYSNKIKEINKSIEVYEVACPELVPLIEKGFYKTESVKRVVIECVKNLKEEKIDT
CHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHE
LVLGCTHYPIVIDYIEEALIGENVRIVDPAERLAYDVKEYITRVNMINPNGSGQVQFYLS
EEEECCCCHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHEEECCCCCCEEEHHHH
KMTQSFIEIAKILLEGKINNYNVSIVDITKY
HHHHHHHHHHHHHHHCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336