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The map label for this gene is GidA

Identifier: 20807573

GI number: 20807573

Start: 1110703

End: 1112604

Strand: Direct

Name: GidA

Synonym: TTE1110

Alternate gene names: 20807573

Gene position: 1110703-1112604 (Clockwise)

Preceding gene: 20807572

Following gene: 20807574

Centisome position: 41.3

GC content: 41.11

Gene sequence:

>1902_bases
ATGAGGTTTGTTGCTGGAGAATACGATGTTTGTGTAGTTGGTTTGGGACATGCTGGCAGTGAAGCTGCCTTGGCCTCTGC
TAGACTTGGACTTAGTACTGTGGGGTTTGCCACAAATCTGGATGCGATAGCTCTCATGGCCTGTAATCCTTCCATTGGAG
GTCCTGCAAAGGCTCAGCTAGTGAGGGAAATTGACGCTTTGGGAGGCCAGATGGCCATAAACACTGACAAGTCTCTTTTG
CAGATGAGGACATTAAATACCAGTAAAGGGCCGGCTGTTCGCTCTTTAAGGGCCCAGGTTGACAAAAAGCTTTATCAGAT
GAATATGAAACACACCCTTGAAAGGCAGGAAAATCTCGATATTAAGCAGGCAGAGATTGTGGACATATTGGTGGAAGACA
ATAAAGTTACGGGGGTTGTTACAAAATTAGGTGCCATTTACAAATGTAAAGCCTGCATTATAACTACAGGTACTTTCCTA
AAAGGAAGAGTGATAATTGGAGAAGTGGACTTTGAAAGTGGTCCCAGCGGTCTTTTCCCGGCAAGCGAGTTATCTGAGGC
TTTAAAAAGATTGGGATTTAAATTGATGAGATTTAAGACAGGGACTCCTCCAAGAGTGGACAAGAGAAGTATTGACTTTT
CTAAGATGGAAATACAGCCGGGAGATGAGGTTATAACTCCCTTTTCTTTCATGCACGATAAAATAGAAATTGAACAAATG
CCCTGCTGGCTCACTTATACCAATGAGAAGACTCACAAAATAATAAGAGATAACATTCACAGAGCTCCTCTTTTTACGGG
GGCCATAACGGGAGTTGGAGTCAGGTATTGTCCCTCCATTGAAGATAAAGTTGTGAAGTTTCCTCACAGAGAAAGACATC
AGATTTTTATTGAGCCAGAAGGCAGGGATACCTATGAAATGTACGTGCAGGGAATGTCCTCCAGCCTTCCTGAGGATGTC
CAGTTAGAATTTTTGAGGACAGTTCCAGGGCTTGAAAATGTGAGGGTAATGAGACCTGCTTATGCCATAGAGTATGACTG
CATAGACCCAACTCAATTAAAAGCTACTTTAGAGTCTAAGTGGATAGAGGGCCTTTATTTTGCTGGTCAAGTAAATGGGA
CATCTGGATATGAAGAGGCGGCGGCTCAAGGGTTGATGGCAGGAATAAACGCTGCCATGAAAATTTTAAACAAACCTCCT
GTAGTTTTGGACCGCTCTCAAGCCTATATAGGCATTCTCATTGATGACTTAGTCACAAAAGGTACAAATGAACCCTACAG
AATGCTAACCTCAAGGGCAGAATACAGATTGTTATTAAGACAGGACAATGCAGATTTTAGATTGACTGAGATAGGAAAAG
AAATAGGGCTTGTGACAGAAGAAAGGTATGAGAAATTTTTGAGGAAGAAAATACAGTTAGAAAAAGAGATGAGAAGGCTT
TCCACAGTCATGGTGAGGCCGACAGAAGAAGTCAACAACTTTTTAATTTCCAGAGGAAGCACTCCTTTAGTTTCGGGTGT
AGACCTGTACACTTTATTGAAGAGACCCGAAGTTGACTACAAATCTACTAAATTTTTAGATCCAGATAGACCCGATGACA
TTTTAGATAGCGTTGCAGAGCAAATCGACATAAACATAAAATATGAAGGGTATATTTTGAAGCAGTTGAGACAAGTGGAA
CAGTTTAAGGCGATGGAGAATAAAAAAATTCCAGAGGACATAGATTACTACCAGATCAGTGGACTCAGCAATGAAGCTAA
AGAAAAACTGTCAAAGATAAGACCTACTTCTGTTGGACAGGCATCCCGTATTTCAGGAGTTTCACCTGCAGATATTTCTG
TACTTTTAATATATTTACAACAGATGAGAAAAAAGAAGTCCAATGAATCCAGAATCAGTTGA

Upstream 100 bases:

>100_bases
CGCATTGAATGAAATTGGAAAAATCACCGGTGAGACAGCTACAGAGGATGTAATAGACCAAATCTTTGAGAGATTTTGTG
TAGGAAAGTAGGTGGTGTTT

Downstream 100 bases:

>100_bases
AATGCTATTGAAATGGAATCAAAAGATGAACTTGGCGGCGAGTACCGAGGAAGAGAAGGTAGTGATAAAACATTTTTTAG
ACAGTTTATCTTTGGTAAGA

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A 1

Number of amino acids: Translated: 633; Mature: 633

Protein sequence:

>633_residues
MRFVAGEYDVCVVGLGHAGSEAALASARLGLSTVGFATNLDAIALMACNPSIGGPAKAQLVREIDALGGQMAINTDKSLL
QMRTLNTSKGPAVRSLRAQVDKKLYQMNMKHTLERQENLDIKQAEIVDILVEDNKVTGVVTKLGAIYKCKACIITTGTFL
KGRVIIGEVDFESGPSGLFPASELSEALKRLGFKLMRFKTGTPPRVDKRSIDFSKMEIQPGDEVITPFSFMHDKIEIEQM
PCWLTYTNEKTHKIIRDNIHRAPLFTGAITGVGVRYCPSIEDKVVKFPHRERHQIFIEPEGRDTYEMYVQGMSSSLPEDV
QLEFLRTVPGLENVRVMRPAYAIEYDCIDPTQLKATLESKWIEGLYFAGQVNGTSGYEEAAAQGLMAGINAAMKILNKPP
VVLDRSQAYIGILIDDLVTKGTNEPYRMLTSRAEYRLLLRQDNADFRLTEIGKEIGLVTEERYEKFLRKKIQLEKEMRRL
STVMVRPTEEVNNFLISRGSTPLVSGVDLYTLLKRPEVDYKSTKFLDPDRPDDILDSVAEQIDINIKYEGYILKQLRQVE
QFKAMENKKIPEDIDYYQISGLSNEAKEKLSKIRPTSVGQASRISGVSPADISVLLIYLQQMRKKKSNESRIS

Sequences:

>Translated_633_residues
MRFVAGEYDVCVVGLGHAGSEAALASARLGLSTVGFATNLDAIALMACNPSIGGPAKAQLVREIDALGGQMAINTDKSLL
QMRTLNTSKGPAVRSLRAQVDKKLYQMNMKHTLERQENLDIKQAEIVDILVEDNKVTGVVTKLGAIYKCKACIITTGTFL
KGRVIIGEVDFESGPSGLFPASELSEALKRLGFKLMRFKTGTPPRVDKRSIDFSKMEIQPGDEVITPFSFMHDKIEIEQM
PCWLTYTNEKTHKIIRDNIHRAPLFTGAITGVGVRYCPSIEDKVVKFPHRERHQIFIEPEGRDTYEMYVQGMSSSLPEDV
QLEFLRTVPGLENVRVMRPAYAIEYDCIDPTQLKATLESKWIEGLYFAGQVNGTSGYEEAAAQGLMAGINAAMKILNKPP
VVLDRSQAYIGILIDDLVTKGTNEPYRMLTSRAEYRLLLRQDNADFRLTEIGKEIGLVTEERYEKFLRKKIQLEKEMRRL
STVMVRPTEEVNNFLISRGSTPLVSGVDLYTLLKRPEVDYKSTKFLDPDRPDDILDSVAEQIDINIKYEGYILKQLRQVE
QFKAMENKKIPEDIDYYQISGLSNEAKEKLSKIRPTSVGQASRISGVSPADISVLLIYLQQMRKKKSNESRIS
>Mature_633_residues
MRFVAGEYDVCVVGLGHAGSEAALASARLGLSTVGFATNLDAIALMACNPSIGGPAKAQLVREIDALGGQMAINTDKSLL
QMRTLNTSKGPAVRSLRAQVDKKLYQMNMKHTLERQENLDIKQAEIVDILVEDNKVTGVVTKLGAIYKCKACIITTGTFL
KGRVIIGEVDFESGPSGLFPASELSEALKRLGFKLMRFKTGTPPRVDKRSIDFSKMEIQPGDEVITPFSFMHDKIEIEQM
PCWLTYTNEKTHKIIRDNIHRAPLFTGAITGVGVRYCPSIEDKVVKFPHRERHQIFIEPEGRDTYEMYVQGMSSSLPEDV
QLEFLRTVPGLENVRVMRPAYAIEYDCIDPTQLKATLESKWIEGLYFAGQVNGTSGYEEAAAQGLMAGINAAMKILNKPP
VVLDRSQAYIGILIDDLVTKGTNEPYRMLTSRAEYRLLLRQDNADFRLTEIGKEIGLVTEERYEKFLRKKIQLEKEMRRL
STVMVRPTEEVNNFLISRGSTPLVSGVDLYTLLKRPEVDYKSTKFLDPDRPDDILDSVAEQIDINIKYEGYILKQLRQVE
QFKAMENKKIPEDIDYYQISGLSNEAKEKLSKIRPTSVGQASRISGVSPADISVLLIYLQQMRKKKSNESRIS

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=642, Percent_Identity=40.9657320872274, Blast_Score=460, Evalue=1e-129,
Organism=Homo sapiens, GI19882217, Length=667, Percent_Identity=39.4302848575712, Blast_Score=446, Evalue=1e-125,
Organism=Homo sapiens, GI183227703, Length=682, Percent_Identity=38.5630498533724, Blast_Score=441, Evalue=1e-123,
Organism=Escherichia coli, GI2367273, Length=620, Percent_Identity=50.3225806451613, Blast_Score=637, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17534255, Length=627, Percent_Identity=39.5534290271132, Blast_Score=460, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6321202, Length=637, Percent_Identity=41.6012558869702, Blast_Score=491, Evalue=1e-139,
Organism=Drosophila melanogaster, GI24658174, Length=640, Percent_Identity=41.875, Blast_Score=463, Evalue=1e-130,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG1_THETN (Q8RAT8)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_622744.1
- ProteinModelPortal:   Q8RAT8
- SMR:   Q8RAT8
- GeneID:   995638
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE1110
- NMPDR:   fig|273068.3.peg.710
- HOGENOM:   HBG284774
- OMA:   GIQFRVL
- ProtClustDB:   PRK05192
- BioCyc:   TTEN273068:TTE1110-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 71073; Mature: 71073

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFVAGEYDVCVVGLGHAGSEAALASARLGLSTVGFATNLDAIALMACNPSIGGPAKAQL
CCCCCCCCCEEEEECCCCCCHHHHHHHHCCCHHHCCCCCCCEEEEEEECCCCCCHHHHHH
VREIDALGGQMAINTDKSLLQMRTLNTSKGPAVRSLRAQVDKKLYQMNMKHTLERQENLD
HHHHHHCCCEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IKQAEIVDILVEDNKVTGVVTKLGAIYKCKACIITTGTFLKGRVIIGEVDFESGPSGLFP
CCCEEEEEEEEECCCHHHHHHHHHHHHEEEEEEEECCCEEECEEEEEEEECCCCCCCCCC
ASELSEALKRLGFKLMRFKTGTPPRVDKRSIDFSKMEIQPGDEVITPFSFMHDKIEIEQM
HHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCEECCHHHHHCCEEEECC
PCWLTYTNEKTHKIIRDNIHRAPLFTGAITGVGVRYCPSIEDKVVKFPHRERHQIFIEPE
CEEEEECCCHHHHHHHHCCCCCCCHHHHHHCCCEEECCCCHHHHHCCCCCCCCEEEEECC
GRDTYEMYVQGMSSSLPEDVQLEFLRTVPGLENVRVMRPAYAIEYDCIDPTQLKATLESK
CCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEECCCEEEEECCCCHHHHHHHHHHH
WIEGLYFAGQVNGTSGYEEAAAQGLMAGINAAMKILNKPPVVLDRSQAYIGILIDDLVTK
HHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEHHHHHHC
GTNEPYRMLTSRAEYRLLLRQDNADFRLTEIGKEIGLVTEERYEKFLRKKIQLEKEMRRL
CCCCHHHHHHCCCCEEEEEEECCCCEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
STVMVRPTEEVNNFLISRGSTPLVSGVDLYTLLKRPEVDYKSTKFLDPDRPDDILDSVAE
HHHEECCHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH
QIDINIKYEGYILKQLRQVEQFKAMENKKIPEDIDYYQISGLSNEAKEKLSKIRPTSVGQ
HEEEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHCCCCCCCC
ASRISGVSPADISVLLIYLQQMRKKKSNESRIS
HHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRFVAGEYDVCVVGLGHAGSEAALASARLGLSTVGFATNLDAIALMACNPSIGGPAKAQL
CCCCCCCCCEEEEECCCCCCHHHHHHHHCCCHHHCCCCCCCEEEEEEECCCCCCHHHHHH
VREIDALGGQMAINTDKSLLQMRTLNTSKGPAVRSLRAQVDKKLYQMNMKHTLERQENLD
HHHHHHCCCEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IKQAEIVDILVEDNKVTGVVTKLGAIYKCKACIITTGTFLKGRVIIGEVDFESGPSGLFP
CCCEEEEEEEEECCCHHHHHHHHHHHHEEEEEEEECCCEEECEEEEEEEECCCCCCCCCC
ASELSEALKRLGFKLMRFKTGTPPRVDKRSIDFSKMEIQPGDEVITPFSFMHDKIEIEQM
HHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCEECCHHHHHCCEEEECC
PCWLTYTNEKTHKIIRDNIHRAPLFTGAITGVGVRYCPSIEDKVVKFPHRERHQIFIEPE
CEEEEECCCHHHHHHHHCCCCCCCHHHHHHCCCEEECCCCHHHHHCCCCCCCCEEEEECC
GRDTYEMYVQGMSSSLPEDVQLEFLRTVPGLENVRVMRPAYAIEYDCIDPTQLKATLESK
CCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEECCCEEEEECCCCHHHHHHHHHHH
WIEGLYFAGQVNGTSGYEEAAAQGLMAGINAAMKILNKPPVVLDRSQAYIGILIDDLVTK
HHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEHHHHHHC
GTNEPYRMLTSRAEYRLLLRQDNADFRLTEIGKEIGLVTEERYEKFLRKKIQLEKEMRRL
CCCCHHHHHHCCCCEEEEEEECCCCEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
STVMVRPTEEVNNFLISRGSTPLVSGVDLYTLLKRPEVDYKSTKFLDPDRPDDILDSVAE
HHHEECCHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH
QIDINIKYEGYILKQLRQVEQFKAMENKKIPEDIDYYQISGLSNEAKEKLSKIRPTSVGQ
HEEEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHCCCCCCCC
ASRISGVSPADISVLLIYLQQMRKKKSNESRIS
HHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336