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The map label for this gene is mnmE [H]

Identifier: 20807571

GI number: 20807571

Start: 1107283

End: 1107564

Strand: Direct

Name: mnmE [H]

Synonym: TTE1105

Alternate gene names: 20807571

Gene position: 1107283-1107564 (Clockwise)

Preceding gene: 20807570

Following gene: 20807572

Centisome position: 41.17

GC content: 36.88

Gene sequence:

>282_bases
ATGGAGTTTGACACAATTGCAGCCATTTCTACTTCTCCTGGTGAAGCAGGGATTGGAATTGTGAGAATGAGTGGTGATGG
GGCTTTAGATATAATTTCAAGAATTTTTAGATTTTATAAGAAAAAAGATGTAAAGAATGTAAAGACCCACACCCTTCATT
ATGGACACATTGTAGACCCGGATACAGGAGAAGTTTACGATGAAGTGTTGGTTTCCATCATGAAAAAACCAAATACTTAT
ACTCGGGAAGACATTGTAGAGATAAATTGCCATGGAGGTTAA

Upstream 100 bases:

>100_bases
AGGGTGGGTAATGGCTCAACCCCTTTTTACACAATTATATAGACTTAACTCGCAATAAATTGGTAATTATATATGGATAA
ATTTTGGGCTGGTGATGAAA

Downstream 100 bases:

>100_bases
AACAGGTGAAATTAATAGTAAAAAATGATATAATTTTAATTGGGTGATGATAAATGTTATTAACTATAAAAAAGGTTAAA
GAGCTATATGATATAAGCAG

Product: thiophene degradation protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 93; Mature: 93

Protein sequence:

>93_residues
MEFDTIAAISTSPGEAGIGIVRMSGDGALDIISRIFRFYKKKDVKNVKTHTLHYGHIVDPDTGEVYDEVLVSIMKKPNTY
TREDIVEINCHGG

Sequences:

>Translated_93_residues
MEFDTIAAISTSPGEAGIGIVRMSGDGALDIISRIFRFYKKKDVKNVKTHTLHYGHIVDPDTGEVYDEVLVSIMKKPNTY
TREDIVEINCHGG
>Mature_93_residues
MEFDTIAAISTSPGEAGIGIVRMSGDGALDIISRIFRFYKKKDVKNVKTHTLHYGHIVDPDTGEVYDEVLVSIMKKPNTY
TREDIVEINCHGG

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 10327; Mature: 10327

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFDTIAAISTSPGEAGIGIVRMSGDGALDIISRIFRFYKKKDVKNVKTHTLHYGHIVDP
CCCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCEECC
DTGEVYDEVLVSIMKKPNTYTREDIVEINCHGG
CCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCC
>Mature Secondary Structure
MEFDTIAAISTSPGEAGIGIVRMSGDGALDIISRIFRFYKKKDVKNVKTHTLHYGHIVDP
CCCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCEECC
DTGEVYDEVLVSIMKKPNTYTREDIVEINCHGG
CCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]