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The map label for this gene is BioB [H]

Identifier: 20807545

GI number: 20807545

Start: 1071717

End: 1072772

Strand: Direct

Name: BioB [H]

Synonym: TTE1073

Alternate gene names: 20807545

Gene position: 1071717-1072772 (Clockwise)

Preceding gene: 20807544

Following gene: 20807546

Centisome position: 39.85

GC content: 43.18

Gene sequence:

>1056_bases
ATGAAAGTGAAAGAGATTTTAGAAAAAGCGTATCATGAGAGCAATCTCACAAAGGATGAGATAAAACTTTTGCTGATGGC
AGAAGGTGAGGATAAAGATCTTCTTTTCAAAACAGCAGACAGGGTAAGGAAAGAGCACGTTGGCGACGAGGTTTTTTTGA
GGGGACTTATTGAATTTTCAAGCTATTGCAAAAACGACTGTTTTTACTGTGGACTGAGGCGCAGCAACCAGAATGCTCAA
AGGTACAGAATGCAGGAAGATGAGATAGTAGAAGTTGCAAAAAGAGCATATCAAATGGGGTACCGCACTGTGGTGCTGCA
GTCTGGTGAGGACATGTATTACACCAAAGACAGGCTGTGTTCAATCATAAAGAAGATGAAAAGGGTGGTTGACGTTGCTA
TAACACTTTCGATTGGTGAAAGGTCGTATGATGAGTACAAGGTGTTTAAAGATGCCGGGGCGGACAGGTTTCTGATGAGG
TTTGAAACTTCAAATGAAAAGCTATATAGAAAGTATCATCCTGGCATGAGCTTTGAAAACAGGATAGAGTGCCTTAAGTG
GCTCAAAAAACTTGGGTATGAACTTGGGACAGGTTTTTTGATAGGCCTTCCAGGTCAGACGTTGGATGATTTGGCACAGG
ACATAATTCTTGTAAAGGAATTGGATGCAGATATGATAGGCATTGGACCTTTTATTCCTCATCCCCAAACGCCTCTAAAA
GATGCAAAAGAAGGTTCAGTTGATTTGACGCTCAAGAGCATTGCCATTTTGAGGCTTTTGAATCCAGATGCTAATATTCC
GGCAACAACTGCACTTGGCACTTTAGACCCGCTGGGAAGACAAAAGGGGCTCATGTGTGGTGCAAACATTGTAATGCCGA
ACGTCAACAGGCTTGAGTACAAGCTCAAATATGAGCTGTATCCTGGCAAGATTTGCATAAATGAGGATGCGACAAAGTGC
AGAGGATGCATTGAGTCAATCATTGTTTCGGTGGGCAGGAAGGTCGGGCAGGGGAAGGGACAGAGCAGGCACTACAAAAG
AGCTTCTGTGAGCTAA

Upstream 100 bases:

>100_bases
AAAATGCTTTTAAACCCTTTTAAAATGTGGTATAATAAAATCAATTCCGGCAAAGTAGATAGAAATACTCAAAATTTGCT
TTATGACAGGAGAGTTTAAG

Downstream 100 bases:

>100_bases
ATTGTAATGTGTCATAGTTTCATTCAATAGCTTTTTATTTTTGGGCAGGTTATCCCAGGGCAACGAAGAAATGTCAAAAA
GTAAAGAAACGTAATTAGAA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MKVKEILEKAYHESNLTKDEIKLLLMAEGEDKDLLFKTADRVRKEHVGDEVFLRGLIEFSSYCKNDCFYCGLRRSNQNAQ
RYRMQEDEIVEVAKRAYQMGYRTVVLQSGEDMYYTKDRLCSIIKKMKRVVDVAITLSIGERSYDEYKVFKDAGADRFLMR
FETSNEKLYRKYHPGMSFENRIECLKWLKKLGYELGTGFLIGLPGQTLDDLAQDIILVKELDADMIGIGPFIPHPQTPLK
DAKEGSVDLTLKSIAILRLLNPDANIPATTALGTLDPLGRQKGLMCGANIVMPNVNRLEYKLKYELYPGKICINEDATKC
RGCIESIIVSVGRKVGQGKGQSRHYKRASVS

Sequences:

>Translated_351_residues
MKVKEILEKAYHESNLTKDEIKLLLMAEGEDKDLLFKTADRVRKEHVGDEVFLRGLIEFSSYCKNDCFYCGLRRSNQNAQ
RYRMQEDEIVEVAKRAYQMGYRTVVLQSGEDMYYTKDRLCSIIKKMKRVVDVAITLSIGERSYDEYKVFKDAGADRFLMR
FETSNEKLYRKYHPGMSFENRIECLKWLKKLGYELGTGFLIGLPGQTLDDLAQDIILVKELDADMIGIGPFIPHPQTPLK
DAKEGSVDLTLKSIAILRLLNPDANIPATTALGTLDPLGRQKGLMCGANIVMPNVNRLEYKLKYELYPGKICINEDATKC
RGCIESIIVSVGRKVGQGKGQSRHYKRASVS
>Mature_351_residues
MKVKEILEKAYHESNLTKDEIKLLLMAEGEDKDLLFKTADRVRKEHVGDEVFLRGLIEFSSYCKNDCFYCGLRRSNQNAQ
RYRMQEDEIVEVAKRAYQMGYRTVVLQSGEDMYYTKDRLCSIIKKMKRVVDVAITLSIGERSYDEYKVFKDAGADRFLMR
FETSNEKLYRKYHPGMSFENRIECLKWLKKLGYELGTGFLIGLPGQTLDDLAQDIILVKELDADMIGIGPFIPHPQTPLK
DAKEGSVDLTLKSIAILRLLNPDANIPATTALGTLDPLGRQKGLMCGANIVMPNVNRLEYKLKYELYPGKICINEDATKC
RGCIESIIVSVGRKVGQGKGQSRHYKRASVS

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=229, Percent_Identity=26.2008733624454, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 39966; Mature: 39966

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVKEILEKAYHESNLTKDEIKLLLMAEGEDKDLLFKTADRVRKEHVGDEVFLRGLIEFS
CCHHHHHHHHHHHCCCCHHHEEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHH
SYCKNDCFYCGLRRSNQNAQRYRMQEDEIVEVAKRAYQMGYRTVVLQSGEDMYYTKDRLC
HHHCCCCEEEECCCCCCCHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCCCEECHHHHH
SIIKKMKRVVDVAITLSIGERSYDEYKVFKDAGADRFLMRFETSNEKLYRKYHPGMSFEN
HHHHHHHHHHHEEEEEEECCCCHHHHHHHHHCCCCEEEEEEECCCHHHHHHHCCCCCHHH
RIECLKWLKKLGYELGTGFLIGLPGQTLDDLAQDIILVKELDADMIGIGPFIPHPQTPLK
HHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCC
DAKEGSVDLTLKSIAILRLLNPDANIPATTALGTLDPLGRQKGLMCGANIVMPNVNRLEY
CCCCCCEEEEHHHHHHHHEECCCCCCCCHHHCCCCCCCCCCCCCEECCEEECCCCCEEEE
KLKYELYPGKICINEDATKCRGCIESIIVSVGRKVGQGKGQSRHYKRASVS
EEEEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKVKEILEKAYHESNLTKDEIKLLLMAEGEDKDLLFKTADRVRKEHVGDEVFLRGLIEFS
CCHHHHHHHHHHHCCCCHHHEEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHH
SYCKNDCFYCGLRRSNQNAQRYRMQEDEIVEVAKRAYQMGYRTVVLQSGEDMYYTKDRLC
HHHCCCCEEEECCCCCCCHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCCCEECHHHHH
SIIKKMKRVVDVAITLSIGERSYDEYKVFKDAGADRFLMRFETSNEKLYRKYHPGMSFEN
HHHHHHHHHHHEEEEEEECCCCHHHHHHHHHCCCCEEEEEEECCCHHHHHHHCCCCCHHH
RIECLKWLKKLGYELGTGFLIGLPGQTLDDLAQDIILVKELDADMIGIGPFIPHPQTPLK
HHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCC
DAKEGSVDLTLKSIAILRLLNPDANIPATTALGTLDPLGRQKGLMCGANIVMPNVNRLEY
CCCCCCEEEEHHHHHHHHEECCCCCCCCHHHCCCCCCCCCCCCCEECCEEECCCCCEEEE
KLKYELYPGKICINEDATKCRGCIESIIVSVGRKVGQGKGQSRHYKRASVS
EEEEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA