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The map label for this gene is CheB

Identifier: 20807514

GI number: 20807514

Start: 1041694

End: 1042797

Strand: Direct

Name: CheB

Synonym: TTE1035

Alternate gene names: 20807514

Gene position: 1041694-1042797 (Clockwise)

Preceding gene: 20807513

Following gene: 20807515

Centisome position: 38.73

GC content: 39.13

Gene sequence:

>1104_bases
ATGAAGAAAAAAATTCGCGTACTCGTAGTGGATGACTCTGCTTTTATGCGGAGGTGTTTAAAGGATATACTTGAAAATGA
GGAAGACATGGAAGTAATTGATACCGCTCGTGATGGCAATGAGGCAGTAAAGAAGGCAGTAGAACTTCGCCCTGATGTGA
TAACTCTTGATATAAATATGCCAGTAATGGATGGGCTTACTGCGCTGCAGTACATAATGTCTCTTGCGCCATGTCCTGTT
GTAATTATTTCATCTCTTTCTACAGAAGGAGCTTTAACCACATTTGAAGCTCTCGAACTTGGAGCTGTTGATTTTGTGGC
AAAACCAGGAGGCACAGTTTCGCTGGGAATAAAGCAGCTTGCAGACGAAATTGTATCAAAAGTGAGGATAGCTGCCTTAT
CTAATAAGGAGCTACTAAGTTCTTCAAGAAACTTTAAAAAGTTATTGAAACGTAGAAATCAGGAAGTAACTAGGAAAGTA
TATGAAAATAAAATTTCTAAAAAAGAAATTGTGGTAGTAATTGGAGTTTCTACAGGGGGACCGAAAACATTGATGGAAAT
TTTACCTTATCTTCCTTCAGATTTTCCGGCAGCTGTGTTGGTAGTTCAGCACATGCCGCCAGGTTTTACCCAATCTTTTG
CTCAACGTCTAGATCAAAGCTGCAATTTAAAGGTAAAGGAAGCGGAAGACAAAGCGTTTATAGAACCTGGAACAGTTATC
ATTGCAAAAGGTGGATGGCACCTTGTGGTGGAAAGAGATTCAAGAAGTTCTAAGCTTATTACTAGATTAACTCAGAAGCC
TGAAGAGACACTTTATAAGCCTTCTATTAATGTTACCATGAAATCGGTACTTGAAAATGTAGATGGCCGGAATATTATAG
GTATACTTTTGACGGGTATGGGTGACGACGGTGCAGATATGATGGTTGAAATAAGAAAACGAGGTGGATTAACTATTGCA
GAATCACAAGAAACTGCTATAGTATATGGTATGCCAAGAGCAGCTGTAGAGCGTGGTGGAGCTGAGATAGTGGCACCTGC
TTATAAGATAAGTGATATCTTGTTAAAGAAGGTGAATGAGTATGCAAGAACCTCAGAATATTAA

Upstream 100 bases:

>100_bases
CTGTGGAAGAGGTGTTGTCAGGAAAGGAGAAAAAAGAACTAATGGCAATAGAGGAAGCAGATAATTGACGCAAGGGTTTG
ATAGGAGAGAAGTGTTAATT

Downstream 100 bases:

>100_bases
TGAATTAATCAGGCTAATGAAGAACGGCAGAAATGAAAGCGAACGCATCGCGGCAGCACTCGATTTGGGGAATTTTAAAA
GCAAGGAGGTAGTAAAAGCC

Product: methylesterase CheB/methylase CheR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MKKKIRVLVVDDSAFMRRCLKDILENEEDMEVIDTARDGNEAVKKAVELRPDVITLDINMPVMDGLTALQYIMSLAPCPV
VIISSLSTEGALTTFEALELGAVDFVAKPGGTVSLGIKQLADEIVSKVRIAALSNKELLSSSRNFKKLLKRRNQEVTRKV
YENKISKKEIVVVIGVSTGGPKTLMEILPYLPSDFPAAVLVVQHMPPGFTQSFAQRLDQSCNLKVKEAEDKAFIEPGTVI
IAKGGWHLVVERDSRSSKLITRLTQKPEETLYKPSINVTMKSVLENVDGRNIIGILLTGMGDDGADMMVEIRKRGGLTIA
ESQETAIVYGMPRAAVERGGAEIVAPAYKISDILLKKVNEYARTSEY

Sequences:

>Translated_367_residues
MKKKIRVLVVDDSAFMRRCLKDILENEEDMEVIDTARDGNEAVKKAVELRPDVITLDINMPVMDGLTALQYIMSLAPCPV
VIISSLSTEGALTTFEALELGAVDFVAKPGGTVSLGIKQLADEIVSKVRIAALSNKELLSSSRNFKKLLKRRNQEVTRKV
YENKISKKEIVVVIGVSTGGPKTLMEILPYLPSDFPAAVLVVQHMPPGFTQSFAQRLDQSCNLKVKEAEDKAFIEPGTVI
IAKGGWHLVVERDSRSSKLITRLTQKPEETLYKPSINVTMKSVLENVDGRNIIGILLTGMGDDGADMMVEIRKRGGLTIA
ESQETAIVYGMPRAAVERGGAEIVAPAYKISDILLKKVNEYARTSEY
>Mature_367_residues
MKKKIRVLVVDDSAFMRRCLKDILENEEDMEVIDTARDGNEAVKKAVELRPDVITLDINMPVMDGLTALQYIMSLAPCPV
VIISSLSTEGALTTFEALELGAVDFVAKPGGTVSLGIKQLADEIVSKVRIAALSNKELLSSSRNFKKLLKRRNQEVTRKV
YENKISKKEIVVVIGVSTGGPKTLMEILPYLPSDFPAAVLVVQHMPPGFTQSFAQRLDQSCNLKVKEAEDKAFIEPGTVI
IAKGGWHLVVERDSRSSKLITRLTQKPEETLYKPSINVTMKSVLENVDGRNIIGILLTGMGDDGADMMVEIRKRGGLTIA
ESQETAIVYGMPRAAVERGGAEIVAPAYKISDILLKKVNEYARTSEY

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=359, Percent_Identity=39.2757660167131, Blast_Score=247, Evalue=1e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB_THETN (Q8RAZ3)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_622685.1
- ProteinModelPortal:   Q8RAZ3
- SMR:   Q8RAZ3
- GeneID:   995741
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE1035
- NMPDR:   fig|273068.3.peg.546
- HOGENOM:   HBG705324
- OMA:   CQIEVIE
- ProtClustDB:   CLSK569149
- BioCyc:   TTEN273068:TTE1035-MONOMER
- BRENDA:   3.1.1.61
- GO:   GO:0005737
- GO:   GO:0006935
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- PIRSF:   PIRSF000876
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 40351; Mature: 40351

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY; PS00120 LIPASE_SER

Important sites: ACT_SITE 177-177 ACT_SITE 204-204 ACT_SITE 303-303

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKIRVLVVDDSAFMRRCLKDILENEEDMEVIDTARDGNEAVKKAVELRPDVITLDINM
CCCEEEEEEECCHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHCCCCEEEEEECC
PVMDGLTALQYIMSLAPCPVVIISSLSTEGALTTFEALELGAVDFVAKPGGTVSLGIKQL
CHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCEEECCCCEEEHHHHHH
ADEIVSKVRIAALSNKELLSSSRNFKKLLKRRNQEVTRKVYENKISKKEIVVVIGVSTGG
HHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCC
PKTLMEILPYLPSDFPAAVLVVQHMPPGFTQSFAQRLDQSCNLKVKEAEDKAFIEPGTVI
HHHHHHHHHCCCCCCCHHEEEEECCCCCHHHHHHHHHCHHCCEEEEECCCCEEECCCEEE
IAKGGWHLVVERDSRSSKLITRLTQKPEETLYKPSINVTMKSVLENVDGRNIIGILLTGM
EECCCEEEEEECCCCHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHCCCCCEEEEEEEECC
GDDGADMMVEIRKRGGLTIAESQETAIVYGMPRAAVERGGAEIVAPAYKISDILLKKVNE
CCCCHHHHHHHHHCCCCEEECCCCCEEEEECCHHHHHCCCCEEEECHHHHHHHHHHHHHH
YARTSEY
HHHCCCC
>Mature Secondary Structure
MKKKIRVLVVDDSAFMRRCLKDILENEEDMEVIDTARDGNEAVKKAVELRPDVITLDINM
CCCEEEEEEECCHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHCCCCEEEEEECC
PVMDGLTALQYIMSLAPCPVVIISSLSTEGALTTFEALELGAVDFVAKPGGTVSLGIKQL
CHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCEEECCCCEEEHHHHHH
ADEIVSKVRIAALSNKELLSSSRNFKKLLKRRNQEVTRKVYENKISKKEIVVVIGVSTGG
HHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCC
PKTLMEILPYLPSDFPAAVLVVQHMPPGFTQSFAQRLDQSCNLKVKEAEDKAFIEPGTVI
HHHHHHHHHCCCCCCCHHEEEEECCCCCHHHHHHHHHCHHCCEEEEECCCCEEECCCEEE
IAKGGWHLVVERDSRSSKLITRLTQKPEETLYKPSINVTMKSVLENVDGRNIIGILLTGM
EECCCEEEEEECCCCHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHCCCCCEEEEEEEECC
GDDGADMMVEIRKRGGLTIAESQETAIVYGMPRAAVERGGAEIVAPAYKISDILLKKVNE
CCCCHHHHHHHHHCCCCEEECCCCCEEEEECCHHHHHCCCCEEEECHHHHHHHHHHHHHH
YARTSEY
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336