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The map label for this gene is nagB

Identifier: 20807502

GI number: 20807502

Start: 1025315

End: 1026076

Strand: Direct

Name: nagB

Synonym: TTE1023

Alternate gene names: 20807502

Gene position: 1025315-1026076 (Clockwise)

Preceding gene: 20807501

Following gene: 20807503

Centisome position: 38.12

GC content: 35.3

Gene sequence:

>762_bases
ATGAAAGTGATGATAGTAAGAGACTATGATGAAATGAGCAGAAGAGCTGCAGAAATTGTAAAAGAGCAAATTCTGAAAAA
ACCCGACACTGTTTTAGGGCTTGCCACAGGTTCTACTCCTTTGGGCATGTATAAGTATTTAATAGAAATGCATAAAAATG
GTGAAATTGATTTTTCAAATGTAATTACTTTTAATTTAGATGAGTACATAGGACTTTCTCCTGACCATCCGCAAAGCTAT
CATTATTTCATGTATGAAAATTTATTCAATCACATAAATATTAAAAAAGAAAATATACATATTCCAAATGGAGTCGCCGA
AGATTTAGAAGAGGAATGCAAAAGATATGAAAGGGAGATAAGGAGAATAGGCAGGATTGACCTGCAGATACTAGGTATTG
GGGTAAACGGCCATATAGGTTTTAATGAGCCAGATGAAAGCATTGAAACGAAAACCCATATAGTGACGTTGACGGAAGAG
ACTATAAATGCTAATAAGAGGTTTTTCAAAAGCATAGAGGAAGTACCAAGAAGAGCAATCACTATGGGATTGAGCAGTAT
TATGAAGGCAAGAAAAATTATGCTTTTAGCATCAGGAAGTAATAAAGCTAAGGCGATAAAGGAAACTTTAAAGGGGAGAT
TGACAACAAAAGTACCTTCTACTGTTTTAGCCCTACATCCTGATGTCACTATAATAATAGATAAAAGGGCAGCTTCTCTT
CTCACGGATGAGGAGCTAGCCAATGTGGAATTGATAGATTAG

Upstream 100 bases:

>100_bases
CTTTCTGCCTTCTAGACTTTATTATTTACACTTTCCAGCCCTTTTATGGTATAATAGAGCCGAGAAATGAAATAACAGAA
AATTAGTGGGGGTCAAGATG

Downstream 100 bases:

>100_bases
GGCGCAAGCCCTTTTTTATTTTGTATAGCTTTTTACTTTGGGGTATAATAGGTTAGAGGATTACATAAATTGGCTTAATA
GTGGTGAGAAAGATGATAAA

Product: glucosamine-6-phosphate deaminase

Products: NA

Alternate protein names: GlcN6P deaminase; GNPDA; Glucosamine-6-phosphate isomerase

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MKVMIVRDYDEMSRRAAEIVKEQILKKPDTVLGLATGSTPLGMYKYLIEMHKNGEIDFSNVITFNLDEYIGLSPDHPQSY
HYFMYENLFNHINIKKENIHIPNGVAEDLEEECKRYEREIRRIGRIDLQILGIGVNGHIGFNEPDESIETKTHIVTLTEE
TINANKRFFKSIEEVPRRAITMGLSSIMKARKIMLLASGSNKAKAIKETLKGRLTTKVPSTVLALHPDVTIIIDKRAASL
LTDEELANVELID

Sequences:

>Translated_253_residues
MKVMIVRDYDEMSRRAAEIVKEQILKKPDTVLGLATGSTPLGMYKYLIEMHKNGEIDFSNVITFNLDEYIGLSPDHPQSY
HYFMYENLFNHINIKKENIHIPNGVAEDLEEECKRYEREIRRIGRIDLQILGIGVNGHIGFNEPDESIETKTHIVTLTEE
TINANKRFFKSIEEVPRRAITMGLSSIMKARKIMLLASGSNKAKAIKETLKGRLTTKVPSTVLALHPDVTIIIDKRAASL
LTDEELANVELID
>Mature_253_residues
MKVMIVRDYDEMSRRAAEIVKEQILKKPDTVLGLATGSTPLGMYKYLIEMHKNGEIDFSNVITFNLDEYIGLSPDHPQSY
HYFMYENLFNHINIKKENIHIPNGVAEDLEEECKRYEREIRRIGRIDLQILGIGVNGHIGFNEPDESIETKTHIVTLTEE
TINANKRFFKSIEEVPRRAITMGLSSIMKARKIMLLASGSNKAKAIKETLKGRLTTKVPSTVLALHPDVTIIIDKRAASL
LTDEELANVELID

Specific function: Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion

COG id: COG0363

COG function: function code G; 6-phosphogluconolactonase/Glucosamine-6-phosphate isomerase/deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. NagB subfamily

Homologues:

Organism=Homo sapiens, GI13027378, Length=243, Percent_Identity=42.798353909465, Blast_Score=209, Evalue=2e-54,
Organism=Homo sapiens, GI19923881, Length=243, Percent_Identity=41.9753086419753, Blast_Score=206, Evalue=2e-53,
Organism=Escherichia coli, GI1786893, Length=206, Percent_Identity=44.6601941747573, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1789530, Length=229, Percent_Identity=33.6244541484716, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI48994958, Length=236, Percent_Identity=32.6271186440678, Blast_Score=111, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17554876, Length=242, Percent_Identity=45.0413223140496, Blast_Score=212, Evalue=1e-55,
Organism=Drosophila melanogaster, GI24581960, Length=243, Percent_Identity=41.9753086419753, Blast_Score=212, Evalue=2e-55,
Organism=Drosophila melanogaster, GI19920764, Length=243, Percent_Identity=41.9753086419753, Blast_Score=212, Evalue=2e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NAGB_THETN (Q8R5T0)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_622673.1
- ProteinModelPortal:   Q8R5T0
- SMR:   Q8R5T0
- GeneID:   995756
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE1023
- NMPDR:   fig|273068.3.peg.534
- HOGENOM:   HBG725991
- OMA:   HLSITMG
- ProtClustDB:   PRK00443
- BioCyc:   TTEN273068:TTE1023-MONOMER
- BRENDA:   3.5.99.6
- HAMAP:   MF_01241
- InterPro:   IPR006148
- InterPro:   IPR004547
- InterPro:   IPR018321
- PANTHER:   PTHR11280
- TIGRFAMs:   TIGR00502

Pfam domain/function: PF01182 Glucosamine_iso

EC number: =3.5.99.6

Molecular weight: Translated: 28782; Mature: 28782

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS01161 GLC_GALNAC_ISOMERASE

Important sites: ACT_SITE 67-67 ACT_SITE 136-136 ACT_SITE 138-138 ACT_SITE 143-143

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVMIVRDYDEMSRRAAEIVKEQILKKPDTVLGLATGSTPLGMYKYLIEMHKNGEIDFSN
CEEEEEECHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEHH
VITFNLDEYIGLSPDHPQSYHYFMYENLFNHINIKKENIHIPNGVAEDLEEECKRYEREI
EEEEECHHHCCCCCCCCCCCHHHHHHHHHHHCCEEECCEECCCCHHHHHHHHHHHHHHHH
RRIGRIDLQILGIGVNGHIGFNEPDESIETKTHIVTLTEETINANKRFFKSIEEVPRRAI
HHHCCCEEEEEEEEECCCCCCCCCCHHHCCEEEEEEEEHHHHCCHHHHHHHHHHHHHHHH
TMGLSSIMKARKIMLLASGSNKAKAIKETLKGRLTTKVPSTVLALHPDVTIIIDKRAASL
HHHHHHHHHHHEEEEEECCCCHHHHHHHHHHCCEECCCCCEEEEECCCEEEEECCCHHHH
LTDEELANVELID
HCHHHHCCEEECC
>Mature Secondary Structure
MKVMIVRDYDEMSRRAAEIVKEQILKKPDTVLGLATGSTPLGMYKYLIEMHKNGEIDFSN
CEEEEEECHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEHH
VITFNLDEYIGLSPDHPQSYHYFMYENLFNHINIKKENIHIPNGVAEDLEEECKRYEREI
EEEEECHHHCCCCCCCCCCCHHHHHHHHHHHCCEEECCEECCCCHHHHHHHHHHHHHHHH
RRIGRIDLQILGIGVNGHIGFNEPDESIETKTHIVTLTEETINANKRFFKSIEEVPRRAI
HHHCCCEEEEEEEEECCCCCCCCCCHHHCCEEEEEEEEHHHHCCHHHHHHHHHHHHHHHH
TMGLSSIMKARKIMLLASGSNKAKAIKETLKGRLTTKVPSTVLALHPDVTIIIDKRAASL
HHHHHHHHHHHEEEEEECCCCHHHHHHHHHHCCEECCCCCEEEEECCCEEEEECCCHHHH
LTDEELANVELID
HCHHHHCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336