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The map label for this gene is PpsA2 [H]

Identifier: 20807461

GI number: 20807461

Start: 982503

End: 985130

Strand: Direct

Name: PpsA2 [H]

Synonym: TTE0981

Alternate gene names: 20807461

Gene position: 982503-985130 (Clockwise)

Preceding gene: 20807460

Following gene: 20807462

Centisome position: 36.53

GC content: 43.46

Gene sequence:

>2628_bases
ATGACTAAAAAATATGTGTATTTTTTCAATGAAGGCGACGCCTCAATGAGAGACCTCTTGGGAGGAAAAGGTGCAAATTT
AGCCGAAATGACGAGATTGGGATTACCTGTTCCGCCAGGGTTTACAGTTACCACAGAGGCTTGTACAAGATACTATGAAG
ATGGGGAGACAATTGCACCAGAGATAGTTGAGCAGATTCATGAGTACATGAAGAAGTTAGAAGAGGTTACTGGGAAAAAA
TTTGGAGACCCAAATAACCCGCTTCTCGTATCTGTCAGGTCCGGCGCAAGGGTTTCAATGCCTGGAATGATGGATACCAT
TTTAAACCTTGGCTTGAACGACGAGACCGTAGAGGGCCTTGCAAGAGCAACTAACAACGAGAGATTTGCTTATGACAGCT
ACAGAAGGTTCATCCAGATGTTCTCCGACGTAGTTATGGGGATTGACAAGAACAAATTTGAAGCTATTTTAGATGAGATC
AAGGAAAAAAGAGGTGCTAAGTTTGACACAGACCTTACAGCAGAGGATTTAAAAGAAGTTGTAAAGAGATTCAAAGAATT
ATATAAACAGGAAAAGGGAGTAGACTTCCCACAGGACCCTCAAGAGCAGCTTTTGGAAGCTGTGAAGGCTGTTTTCAGGT
CCTGGAACAACCCGAGAGCTATAGTCTACAGAAGGTTAAATGATATACCGAGCGACTGGGGAACAGCAGTAAACGTGCAA
TCAATGGTATTTGGCAACATGGGTAACGATTCTGGTACTGGCGTTGCATTCACAAGAAATCCCGCAACTGGTGAAAAAGC
TCTATTTGGTGAATTCTTGATGAACGCTCAGGGCGAAGACGTGGTTGCAGGTATAAGAACGCCTCAACCAATTTCTACCT
TGAAAGAGACAATGCCAGAAGTTTATAACCAGTTTGTAGAAATTGCTGAGAAGCTGGAGAAACACTACAAAGACATGCAG
GATATAGAGTTTACCATTGAAAGAGGAAAACTCTACATGCTACAGACAAGAAATGGTAAGAGAACTGCTCAGGCCGCTAT
AAAGATAGCTGTTGACCTTGTAAGAGAAGGCCTAATTGACGAAAAGACAGCTGTATTAAGAGTAGATCCAAAACAGCTAG
ACCAGCTCTTGCATCCTACTTTTGAGCCAGAAGCGTTAAAGGCTGCCAAACCTGTTGCCAAAGGATTGCCTGCATCTCCT
GGTGCTGCTTCAGGAAAGGTGTACTTTACAGCTGATGAAGCAGTTGCTGCTGCAAAATCGGGAGAAAAGGTAATACTTGT
AAGGACAGAGACCTCTCCTGACGATATTGAAGGTATGGCCTCCGCAGAAGGAATACTCACAACTCGCGGTGGAATGACAT
CTCACGCTGCTGTTGTAGCAAGAGGAATGGGTACTCCCTGCGTAGTTGGATGTGGAGATGCGAGAATAGACGAGCAGGCG
AAGGTAATGAGAATTGGCGATATAGTTGTGCGCGAAGGAGACTACATATCAATAGATGGTAGCACTGGCAATGTGTACAT
CGGAGAAATAAAGACAGTGACACCTGAAATAAGCGGAGACTTTGCTATACTGATGGGCTGGGCAGACAAGTACAGAAGAC
TGAGAGTCAAAGCAAATGCGGACACGCCAAGAGATGCAAAGATTGCTCTTGAATTTGGCGCAGAGGGAATTGGACTTGCT
AGGACAGAGCACATGTTCTTTGATGAAGAAAGAATTCCTGCTATGAGAGAGATGATTATAGCCAAGACAGAGGAGCAGAG
GAGAAAGGCATTAGAGAAGATACTGCCTATGCAGAGGTCAGATTTTGAACAGCTGTTTGAGATAATGGGCGAATATCCTG
TTACTATAAGGCTTTTGGACCCACCACTTCACGAATTCTTGCCACACCTTGACGAGGAAATAAGGGAACTTGCTGAAGAC
ATGGGCGTTCCATTCGAAGAATTAAAGGCTACAGTAGAGAGCCTGAAAGAGTTCAATCCAATGCTTGGACACAGAGGATG
TAGATTGGCCGTAACATATCCTGAGATAGCTGAAATGCAGACTAGAGCTATAATAGAGGCTGCAATAAATGTGAAGAAGA
GAACCGGCAAAGACGTCAAACCCCAGATAATGATACCACTTGTGGGCGAATTGAAAGAGTTCAAGTACTTGAAAGACATA
ATTGTAAAAGTAGCAGATGAAATAATAAAACAAAGTGGTATTGAACTAAAATACCTTGTAGGTACAATGGTAGAAGTGCC
AAGAGCAGCATTAACTGCAGATAAGATCGCTAAAGAAGCTGAATTCTTCTCCTTTGGTACAAACGACTTAACTCAGATGA
CCTTCGGATTCTCAAGGGATGACGCTGGTAAATTCTTGGAGACTTACTATGAGAAAAAGATATACGAATTTGACCCATTT
GAAAAGTTAGATCAGGAAGGTGTAGGCAAACTTGTAGAGATGGGCACAAAGCTTGGCAAGCAGACGAGACCAGACCTCGA
AGTAGGAATATGCGGAGAGCACGGTGGAGACCCATCTTCCATAGAGTTCTGCCACAGAGTGGGATTAGACTATGTATCTG
CTTCACCTTACAGAGTGCCTATTGCAAGATTGGCAGCTGCACAGGCAGCTATAAAATACGGAAATTAA

Upstream 100 bases:

>100_bases
TGATGCAAAAGTTAGGCTGCACAGTGATAGATGTCACCAATAAGGCTGTAGAGGAGACAGCTAACATAATATTAAACGTG
TTAAGAGGAGGAGAGAGTAC

Downstream 100 bases:

>100_bases
CTTACAAAGGCAGCTTTTGCGCTGCCTTTGTATTGTTTTTTCAGAGAACATGTTATAATATTAAGTAAAAATGGCAATTT
AATAATGGAGGGATTAAAAT

Product: pyruvate phosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 875; Mature: 874

Protein sequence:

>875_residues
MTKKYVYFFNEGDASMRDLLGGKGANLAEMTRLGLPVPPGFTVTTEACTRYYEDGETIAPEIVEQIHEYMKKLEEVTGKK
FGDPNNPLLVSVRSGARVSMPGMMDTILNLGLNDETVEGLARATNNERFAYDSYRRFIQMFSDVVMGIDKNKFEAILDEI
KEKRGAKFDTDLTAEDLKEVVKRFKELYKQEKGVDFPQDPQEQLLEAVKAVFRSWNNPRAIVYRRLNDIPSDWGTAVNVQ
SMVFGNMGNDSGTGVAFTRNPATGEKALFGEFLMNAQGEDVVAGIRTPQPISTLKETMPEVYNQFVEIAEKLEKHYKDMQ
DIEFTIERGKLYMLQTRNGKRTAQAAIKIAVDLVREGLIDEKTAVLRVDPKQLDQLLHPTFEPEALKAAKPVAKGLPASP
GAASGKVYFTADEAVAAAKSGEKVILVRTETSPDDIEGMASAEGILTTRGGMTSHAAVVARGMGTPCVVGCGDARIDEQA
KVMRIGDIVVREGDYISIDGSTGNVYIGEIKTVTPEISGDFAILMGWADKYRRLRVKANADTPRDAKIALEFGAEGIGLA
RTEHMFFDEERIPAMREMIIAKTEEQRRKALEKILPMQRSDFEQLFEIMGEYPVTIRLLDPPLHEFLPHLDEEIRELAED
MGVPFEELKATVESLKEFNPMLGHRGCRLAVTYPEIAEMQTRAIIEAAINVKKRTGKDVKPQIMIPLVGELKEFKYLKDI
IVKVADEIIKQSGIELKYLVGTMVEVPRAALTADKIAKEAEFFSFGTNDLTQMTFGFSRDDAGKFLETYYEKKIYEFDPF
EKLDQEGVGKLVEMGTKLGKQTRPDLEVGICGEHGGDPSSIEFCHRVGLDYVSASPYRVPIARLAAAQAAIKYGN

Sequences:

>Translated_875_residues
MTKKYVYFFNEGDASMRDLLGGKGANLAEMTRLGLPVPPGFTVTTEACTRYYEDGETIAPEIVEQIHEYMKKLEEVTGKK
FGDPNNPLLVSVRSGARVSMPGMMDTILNLGLNDETVEGLARATNNERFAYDSYRRFIQMFSDVVMGIDKNKFEAILDEI
KEKRGAKFDTDLTAEDLKEVVKRFKELYKQEKGVDFPQDPQEQLLEAVKAVFRSWNNPRAIVYRRLNDIPSDWGTAVNVQ
SMVFGNMGNDSGTGVAFTRNPATGEKALFGEFLMNAQGEDVVAGIRTPQPISTLKETMPEVYNQFVEIAEKLEKHYKDMQ
DIEFTIERGKLYMLQTRNGKRTAQAAIKIAVDLVREGLIDEKTAVLRVDPKQLDQLLHPTFEPEALKAAKPVAKGLPASP
GAASGKVYFTADEAVAAAKSGEKVILVRTETSPDDIEGMASAEGILTTRGGMTSHAAVVARGMGTPCVVGCGDARIDEQA
KVMRIGDIVVREGDYISIDGSTGNVYIGEIKTVTPEISGDFAILMGWADKYRRLRVKANADTPRDAKIALEFGAEGIGLA
RTEHMFFDEERIPAMREMIIAKTEEQRRKALEKILPMQRSDFEQLFEIMGEYPVTIRLLDPPLHEFLPHLDEEIRELAED
MGVPFEELKATVESLKEFNPMLGHRGCRLAVTYPEIAEMQTRAIIEAAINVKKRTGKDVKPQIMIPLVGELKEFKYLKDI
IVKVADEIIKQSGIELKYLVGTMVEVPRAALTADKIAKEAEFFSFGTNDLTQMTFGFSRDDAGKFLETYYEKKIYEFDPF
EKLDQEGVGKLVEMGTKLGKQTRPDLEVGICGEHGGDPSSIEFCHRVGLDYVSASPYRVPIARLAAAQAAIKYGN
>Mature_874_residues
TKKYVYFFNEGDASMRDLLGGKGANLAEMTRLGLPVPPGFTVTTEACTRYYEDGETIAPEIVEQIHEYMKKLEEVTGKKF
GDPNNPLLVSVRSGARVSMPGMMDTILNLGLNDETVEGLARATNNERFAYDSYRRFIQMFSDVVMGIDKNKFEAILDEIK
EKRGAKFDTDLTAEDLKEVVKRFKELYKQEKGVDFPQDPQEQLLEAVKAVFRSWNNPRAIVYRRLNDIPSDWGTAVNVQS
MVFGNMGNDSGTGVAFTRNPATGEKALFGEFLMNAQGEDVVAGIRTPQPISTLKETMPEVYNQFVEIAEKLEKHYKDMQD
IEFTIERGKLYMLQTRNGKRTAQAAIKIAVDLVREGLIDEKTAVLRVDPKQLDQLLHPTFEPEALKAAKPVAKGLPASPG
AASGKVYFTADEAVAAAKSGEKVILVRTETSPDDIEGMASAEGILTTRGGMTSHAAVVARGMGTPCVVGCGDARIDEQAK
VMRIGDIVVREGDYISIDGSTGNVYIGEIKTVTPEISGDFAILMGWADKYRRLRVKANADTPRDAKIALEFGAEGIGLAR
TEHMFFDEERIPAMREMIIAKTEEQRRKALEKILPMQRSDFEQLFEIMGEYPVTIRLLDPPLHEFLPHLDEEIRELAEDM
GVPFEELKATVESLKEFNPMLGHRGCRLAVTYPEIAEMQTRAIIEAAINVKKRTGKDVKPQIMIPLVGELKEFKYLKDII
VKVADEIIKQSGIELKYLVGTMVEVPRAALTADKIAKEAEFFSFGTNDLTQMTFGFSRDDAGKFLETYYEKKIYEFDPFE
KLDQEGVGKLVEMGTKLGKQTRPDLEVGICGEHGGDPSSIEFCHRVGLDYVSASPYRVPIARLAAAQAAIKYGN

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate. In E.histolytica and C.symbiosus, PPDK functions in the direction of ATP synthesis [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=460, Percent_Identity=28.2608695652174, Blast_Score=135, Evalue=9e-33,
Organism=Escherichia coli, GI1787994, Length=917, Percent_Identity=23.5550708833152, Blast_Score=110, Evalue=4e-25,
Organism=Escherichia coli, GI1788726, Length=466, Percent_Identity=25.5364806866953, Blast_Score=107, Evalue=4e-24,
Organism=Escherichia coli, GI48994992, Length=440, Percent_Identity=23.1818181818182, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1789193, Length=479, Percent_Identity=24.0083507306889, Blast_Score=99, Evalue=1e-21,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 97442; Mature: 97311

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKYVYFFNEGDASMRDLLGGKGANLAEMTRLGLPVPPGFTVTTEACTRYYEDGETIAP
CCCEEEEEEECCCHHHHHHHCCCCCCHHHHHHCCCCCCCCCEEEHHHHHHHHHCCCCHHH
EIVEQIHEYMKKLEEVTGKKFGDPNNPLLVSVRSGARVSMPGMMDTILNLGLNDETVEGL
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEECCHHHHHHHHCCCCHHHHHHH
ARATNNERFAYDSYRRFIQMFSDVVMGIDKNKFEAILDEIKEKRGAKFDTDLTAEDLKEV
HHHCCCCCEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
VKRFKELYKQEKGVDFPQDPQEQLLEAVKAVFRSWNNPRAIVYRRLNDIPSDWGTAVNVQ
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCEEEH
SMVFGNMGNDSGTGVAFTRNPATGEKALFGEFLMNAQGEDVVAGIRTPQPISTLKETMPE
HHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHH
VYNQFVEIAEKLEKHYKDMQDIEFTIERGKLYMLQTRNGKRTAQAAIKIAVDLVREGLID
HHHHHHHHHHHHHHHHCCHHHHHEEEECCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCC
EKTAVLRVDPKQLDQLLHPTFEPEALKAAKPVAKGLPASPGAASGKVYFTADEAVAAAKS
CCCEEEEECHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHHCC
GEKVILVRTETSPDDIEGMASAEGILTTRGGMTSHAAVVARGMGTPCVVGCGDARIDEQA
CCEEEEEEECCCCHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCCCEEEECCCCCCCCHH
KVMRIGDIVVREGDYISIDGSTGNVYIGEIKTVTPEISGDFAILMGWADKYRRLRVKANA
HHEEECCEEEECCCEEEEECCCCCEEEEEEEEECCCCCCCEEEEEECHHHCEEEEEEECC
DTPRDAKIALEFGAEGIGLARTEHMFFDEERIPAMREMIIAKTEEQRRKALEKILPMQRS
CCCCCCEEEEEECCCCCCCEEHHHHHCCHHCCHHHHHHHHHCCHHHHHHHHHHHCCCCCC
DFEQLFEIMGEYPVTIRLLDPPLHEFLPHLDEEIRELAEDMGVPFEELKATVESLKEFNP
CHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCH
MLGHRGCRLAVTYPEIAEMQTRAIIEAAINVKKRTGKDVKPQIMIPLVGELKEFKYLKDI
HHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHH
IVKVADEIIKQSGIELKYLVGTMVEVPRAALTADKIAKEAEFFSFGTNDLTQMTFGFSRD
HHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCC
DAGKFLETYYEKKIYEFDPFEKLDQEGVGKLVEMGTKLGKQTRPDLEVGICGEHGGDPSS
HHHHHHHHHHHHHCCCCCCHHHHCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHH
IEFCHRVGLDYVSASPYRVPIARLAAAQAAIKYGN
HHHHHHCCCHHCCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TKKYVYFFNEGDASMRDLLGGKGANLAEMTRLGLPVPPGFTVTTEACTRYYEDGETIAP
CCEEEEEEECCCHHHHHHHCCCCCCHHHHHHCCCCCCCCCEEEHHHHHHHHHCCCCHHH
EIVEQIHEYMKKLEEVTGKKFGDPNNPLLVSVRSGARVSMPGMMDTILNLGLNDETVEGL
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEECCHHHHHHHHCCCCHHHHHHH
ARATNNERFAYDSYRRFIQMFSDVVMGIDKNKFEAILDEIKEKRGAKFDTDLTAEDLKEV
HHHCCCCCEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
VKRFKELYKQEKGVDFPQDPQEQLLEAVKAVFRSWNNPRAIVYRRLNDIPSDWGTAVNVQ
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCEEEH
SMVFGNMGNDSGTGVAFTRNPATGEKALFGEFLMNAQGEDVVAGIRTPQPISTLKETMPE
HHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHH
VYNQFVEIAEKLEKHYKDMQDIEFTIERGKLYMLQTRNGKRTAQAAIKIAVDLVREGLID
HHHHHHHHHHHHHHHHCCHHHHHEEEECCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCC
EKTAVLRVDPKQLDQLLHPTFEPEALKAAKPVAKGLPASPGAASGKVYFTADEAVAAAKS
CCCEEEEECHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHHCC
GEKVILVRTETSPDDIEGMASAEGILTTRGGMTSHAAVVARGMGTPCVVGCGDARIDEQA
CCEEEEEEECCCCHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCCCEEEECCCCCCCCHH
KVMRIGDIVVREGDYISIDGSTGNVYIGEIKTVTPEISGDFAILMGWADKYRRLRVKANA
HHEEECCEEEECCCEEEEECCCCCEEEEEEEEECCCCCCCEEEEEECHHHCEEEEEEECC
DTPRDAKIALEFGAEGIGLARTEHMFFDEERIPAMREMIIAKTEEQRRKALEKILPMQRS
CCCCCCEEEEEECCCCCCCEEHHHHHCCHHCCHHHHHHHHHCCHHHHHHHHHHHCCCCCC
DFEQLFEIMGEYPVTIRLLDPPLHEFLPHLDEEIRELAEDMGVPFEELKATVESLKEFNP
CHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCH
MLGHRGCRLAVTYPEIAEMQTRAIIEAAINVKKRTGKDVKPQIMIPLVGELKEFKYLKDI
HHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHH
IVKVADEIIKQSGIELKYLVGTMVEVPRAALTADKIAKEAEFFSFGTNDLTQMTFGFSRD
HHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCC
DAGKFLETYYEKKIYEFDPFEKLDQEGVGKLVEMGTKLGKQTRPDLEVGICGEHGGDPSS
HHHHHHHHHHHHHCCCCCCHHHHCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHH
IEFCHRVGLDYVSASPYRVPIARLAAAQAAIKYGN
HHHHHHCCCHHCCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2176881; 6257292; 11695893; 11468288; 8610096; 9753432 [H]