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The map label for this gene is RfaG8 [C]

Identifier: 20807430

GI number: 20807430

Start: 952812

End: 953936

Strand: Direct

Name: RfaG8 [C]

Synonym: TTE0949

Alternate gene names: 20807430

Gene position: 952812-953936 (Clockwise)

Preceding gene: 20807429

Following gene: 20807431

Centisome position: 35.43

GC content: 37.24

Gene sequence:

>1125_bases
GTGGTTTTTATGGATAAAAGGAGAGTGTTATACATTGTGAGAAAGTCTGAAGGAGGGATGAAGAGGCATTTGCTCTCCCT
TCTTCGCTTTTTAGATAAAGAGAAGTATTGTTTAGGTGTTGTATGCTCGTTTGAAAAAGATACTATTGACTATCTCAAAA
ATCAGGGGATAGAGGTTTTTGAACTTGAAATTGGAGATGGCTTCAATGTAAAAGAGGACCTAAGTATTATTAAGAAAATA
AGGAATATAGTAAAAGAGTTTAAAGCTGACATTGTGCACATGCACGGTTTTAAGGCATCTTTTGTCGGCAGGATTGCCTG
CTTTAATTTGCCTGTAAAAACCATTGTGACGTTTCACAATTTTCCTGGCTACAACAATATGAGCGAAATGAAAAGAAGAG
CGCTTCTTTTTTTGATAAAGTTTTTAAATAAAAGGACTGATCAGTTTATCGCTGTATCTGAAGCCCTGAAAAAAGAGATG
ATGGCAAGCGAAAGGATAGAGGAAGACAGAATAGAAGTGATTTATAACTGTATTGACGAGACTCTTTATAAGATGGGAGA
ATTGGATTTAAGAAAGGAGTTTAATTTACCTGAAGACTCCTTTATTGTAGGAAGTATTGCCAGACTCATCCCCTCAAAAG
GAGTACAGGATTTGATAGAAGCTGCTCATCTAATAAAGGAGGCAGATGTTTTCTTTTTTGTGGCGGGGGATGGCCCCTAT
AGAAAAAGTTTGGAGGAAAAGATTAAGGAAAAAGGGCTTGAAAGCAGGTTTTTTTTGCTCGGCTTTAGAGATGACATACC
TTCATTTTTGAGAAATTTAGATGTTTTTGTACTTCCTTCCCATGAGGAAGGATTTGGAATATCTGTAATAGAAGCGATGA
ATGAAGGAGTGCCGGTTGTGGCTACAGCCGTTGGCGGAATACCTGAGATAATACAAGAAGGCGTAAATGGCATTTTGGTA
GAAAAAGGAAATATAGAATCACTTTCTAAAGCTATAAAGAGCCTTCTTAAAGATGCTCACCTTAAAGAGACGCTTTCTCT
TAAAGGCAAAGAGGCGGCGAAAAAATTTAGCTGTGAAGAGATGGTCAAAAGGGTTGAAGAATTGTATGAGAGAATAAAGG
GTTGA

Upstream 100 bases:

>100_bases
GGCTTTTAATGATTCTGGGGTGATAGCTGCTTCTACTATGTCGGTGTTTTTTGGACCTCTTTTTCTTCACCTTGTGATTG
ATAAATTAAAACGAGGATAG

Downstream 100 bases:

>100_bases
GGGAGTATGAGGCTTACCAGGACAAAAGAAAGGAAAAGGCAAATTAGAAGAAAAAGACTTGTAATATTTGCGCTTTTGTT
GTACATTGTTATTGTGATAA

Product: glycosyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MVFMDKRRVLYIVRKSEGGMKRHLLSLLRFLDKEKYCLGVVCSFEKDTIDYLKNQGIEVFELEIGDGFNVKEDLSIIKKI
RNIVKEFKADIVHMHGFKASFVGRIACFNLPVKTIVTFHNFPGYNNMSEMKRRALLFLIKFLNKRTDQFIAVSEALKKEM
MASERIEEDRIEVIYNCIDETLYKMGELDLRKEFNLPEDSFIVGSIARLIPSKGVQDLIEAAHLIKEADVFFFVAGDGPY
RKSLEEKIKEKGLESRFFLLGFRDDIPSFLRNLDVFVLPSHEEGFGISVIEAMNEGVPVVATAVGGIPEIIQEGVNGILV
EKGNIESLSKAIKSLLKDAHLKETLSLKGKEAAKKFSCEEMVKRVEELYERIKG

Sequences:

>Translated_374_residues
MVFMDKRRVLYIVRKSEGGMKRHLLSLLRFLDKEKYCLGVVCSFEKDTIDYLKNQGIEVFELEIGDGFNVKEDLSIIKKI
RNIVKEFKADIVHMHGFKASFVGRIACFNLPVKTIVTFHNFPGYNNMSEMKRRALLFLIKFLNKRTDQFIAVSEALKKEM
MASERIEEDRIEVIYNCIDETLYKMGELDLRKEFNLPEDSFIVGSIARLIPSKGVQDLIEAAHLIKEADVFFFVAGDGPY
RKSLEEKIKEKGLESRFFLLGFRDDIPSFLRNLDVFVLPSHEEGFGISVIEAMNEGVPVVATAVGGIPEIIQEGVNGILV
EKGNIESLSKAIKSLLKDAHLKETLSLKGKEAAKKFSCEEMVKRVEELYERIKG
>Mature_374_residues
MVFMDKRRVLYIVRKSEGGMKRHLLSLLRFLDKEKYCLGVVCSFEKDTIDYLKNQGIEVFELEIGDGFNVKEDLSIIKKI
RNIVKEFKADIVHMHGFKASFVGRIACFNLPVKTIVTFHNFPGYNNMSEMKRRALLFLIKFLNKRTDQFIAVSEALKKEM
MASERIEEDRIEVIYNCIDETLYKMGELDLRKEFNLPEDSFIVGSIARLIPSKGVQDLIEAAHLIKEADVFFFVAGDGPY
RKSLEEKIKEKGLESRFFLLGFRDDIPSFLRNLDVFVLPSHEEGFGISVIEAMNEGVPVVATAVGGIPEIIQEGVNGILV
EKGNIESLSKAIKSLLKDAHLKETLSLKGKEAAKKFSCEEMVKRVEELYERIKG

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Homo sapiens, GI11863130, Length=375, Percent_Identity=25.3333333333333, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1788357, Length=138, Percent_Identity=34.0579710144928, Blast_Score=72, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17532709, Length=374, Percent_Identity=25.1336898395722, Blast_Score=93, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI9755344, Length=370, Percent_Identity=25.9459459459459, Blast_Score=101, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24654571, Length=321, Percent_Identity=25.2336448598131, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 42679; Mature: 42679

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFMDKRRVLYIVRKSEGGMKRHLLSLLRFLDKEKYCLGVVCSFEKDTIDYLKNQGIEVF
CCEECCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHCCCCEEE
ELEIGDGFNVKEDLSIIKKIRNIVKEFKADIVHMHGFKASFVGRIACFNLPVKTIVTFHN
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHEECC
FPGYNNMSEMKRRALLFLIKFLNKRTDQFIAVSEALKKEMMASERIEEDRIEVIYNCIDE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLYKMGELDLRKEFNLPEDSFIVGSIARLIPSKGVQDLIEAAHLIKEADVFFFVAGDGPY
HHHHHCCHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEEEECCCHH
RKSLEEKIKEKGLESRFFLLGFRDDIPSFLRNLDVFVLPSHEEGFGISVIEAMNEGVPVV
HHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHCCCCCEE
ATAVGGIPEIIQEGVNGILVEKGNIESLSKAIKSLLKDAHLKETLSLKGKEAAKKFSCEE
HHHHCCHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHH
MVKRVEELYERIKG
HHHHHHHHHHHHCC
>Mature Secondary Structure
MVFMDKRRVLYIVRKSEGGMKRHLLSLLRFLDKEKYCLGVVCSFEKDTIDYLKNQGIEVF
CCEECCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHCCCCEEE
ELEIGDGFNVKEDLSIIKKIRNIVKEFKADIVHMHGFKASFVGRIACFNLPVKTIVTFHN
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHEECC
FPGYNNMSEMKRRALLFLIKFLNKRTDQFIAVSEALKKEMMASERIEEDRIEVIYNCIDE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLYKMGELDLRKEFNLPEDSFIVGSIARLIPSKGVQDLIEAAHLIKEADVFFFVAGDGPY
HHHHHCCHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEEEECCCHH
RKSLEEKIKEKGLESRFFLLGFRDDIPSFLRNLDVFVLPSHEEGFGISVIEAMNEGVPVV
HHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHCCCCCEE
ATAVGGIPEIIQEGVNGILVEKGNIESLSKAIKSLLKDAHLKETLSLKGKEAAKKFSCEE
HHHHCCHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHH
MVKRVEELYERIKG
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]