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The map label for this gene is NapF2 [H]

Identifier: 20807368

GI number: 20807368

Start: 897654

End: 898949

Strand: Direct

Name: NapF2 [H]

Synonym: TTE0887

Alternate gene names: 20807368

Gene position: 897654-898949 (Clockwise)

Preceding gene: 20807367

Following gene: 20807369

Centisome position: 33.38

GC content: 38.73

Gene sequence:

>1296_bases
ATGACTTATTTTCATTCTGTGACTTTGGATAAGGACAGGTGCAGAGGATGCACCAACTGCATAAAGAGGTGTCCCACGGA
GGCCATAAGGGTAAGAGAAGGGAAGGCCAGGATTATAAATGAAAGATGTATTGACTGTGGAGAATGCATAAGAGTCTGCC
CATATCATGCCAAACTGGCTGTTACGGATAGTTTGGAAATTATGAAAAATTTCAGGTATAAAATCGCTCTTCCTGCTCCT
TCTCTATACGGGCAGTTTAAAGACTTGTCTATCAACCAGATATTGAGTGCTCTTTTAGATATTGGATTTGATGAGGTGTT
TGAAGTAGCTTATGCTGCAGAGATTGTTTCTAAATTTACAAGAGAAGCTTTACAGAAGGGGAATCTGAAAAAACCGGTAA
TTTCTTCTGCATGTCCTGCTGTTGTAAGGCTCATACAGATTAGGTTTCCATCTCTTATTGACCATCTTCTTAATATATGT
TCTCCTATGGACACAGCTGCAATAATTGCAAAAAAACAGGCAGTGAAGAAAACAGGGCTTAAAGAAGAAGAAATAGGAGT
TTTTTTTATATCTCCCTGTGCTGCTAAAGTGACAAGCATAAGAAATCCAGTAGGCCTAGAGAAATCCAAGATTGATGGAG
CTTTTTCAATGAAGGAAATATACGGGAAAATACTTGAAAAAGCGAAAACTACTGTAGTAAGAGATTTGAGCAAAGCTACC
ATGATAGGAGTAGGATGGGCTAATTCAGGAGGAGAAGCTTTTGGTACTTTTACAGAAAATAGCATATATGTAGATGGGAT
ACACAATGTCATTGATGTCTTAGAAGAGATAGAACTTGGGAAATTGAGTGATTTAGATTTTTTTGAGGGGCTAGCGTGCA
TAGGCGGATGTATAGGAGGACCTCTGACAGTTGAGAATCCTTTTGTTGCTAAAAATAGAATAAGGAAGTTGGCTGAAAGT
CTGCCAAAGAGGGAACAACCTCCCGTAGATGAAGACCTTGTAAGTTTTGAAGAGGTGAAGTGGATAAAGAAAATAGAAAA
AAGTGAAGTGATGAAGCTAGACAAAGATATTTCTAAAGCACTGGAAATGATGAAAGAAATAGACAGAACTCACAAGACTT
TGCCAGGTTTAGACTGCGGATCTTGCGGGTCTCCCAGTTGCAGGGCTTTAGCAGAGGACATTGTAAAAGGCTGTGCTACA
GAAAATGACTGTATCTTCATTTTGAAGGATAAAATAAAAGCCTTAGCGGAAGAGTTAAATGATCTAGCGGGGAAAAAACC
ACCTGTTATTAAGTAA

Upstream 100 bases:

>100_bases
ACTTCCCAATATAAAGGCAAACTCGGATTTTATGGATATAAAAAGCAGTAAAGATGGCACTGTTGTAGTGGCAGAAATAT
TGTTTTAAGAGGTGTGTAAG

Downstream 100 bases:

>100_bases
AAAGGAGAGGAGAATGTATGGGTATTAAAGTAGAAAATCTTCTTAAAAAAGGTTTTTCATTAGTAGCGGGAGAGAAGGGG
ATCGATAGAGAAATTGAGGG

Product: ferredoxin 2

Products: NA

Alternate protein names: Fe hydrogenlyase [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MTYFHSVTLDKDRCRGCTNCIKRCPTEAIRVREGKARIINERCIDCGECIRVCPYHAKLAVTDSLEIMKNFRYKIALPAP
SLYGQFKDLSINQILSALLDIGFDEVFEVAYAAEIVSKFTREALQKGNLKKPVISSACPAVVRLIQIRFPSLIDHLLNIC
SPMDTAAIIAKKQAVKKTGLKEEEIGVFFISPCAAKVTSIRNPVGLEKSKIDGAFSMKEIYGKILEKAKTTVVRDLSKAT
MIGVGWANSGGEAFGTFTENSIYVDGIHNVIDVLEEIELGKLSDLDFFEGLACIGGCIGGPLTVENPFVAKNRIRKLAES
LPKREQPPVDEDLVSFEEVKWIKKIEKSEVMKLDKDISKALEMMKEIDRTHKTLPGLDCGSCGSPSCRALAEDIVKGCAT
ENDCIFILKDKIKALAEELNDLAGKKPPVIK

Sequences:

>Translated_431_residues
MTYFHSVTLDKDRCRGCTNCIKRCPTEAIRVREGKARIINERCIDCGECIRVCPYHAKLAVTDSLEIMKNFRYKIALPAP
SLYGQFKDLSINQILSALLDIGFDEVFEVAYAAEIVSKFTREALQKGNLKKPVISSACPAVVRLIQIRFPSLIDHLLNIC
SPMDTAAIIAKKQAVKKTGLKEEEIGVFFISPCAAKVTSIRNPVGLEKSKIDGAFSMKEIYGKILEKAKTTVVRDLSKAT
MIGVGWANSGGEAFGTFTENSIYVDGIHNVIDVLEEIELGKLSDLDFFEGLACIGGCIGGPLTVENPFVAKNRIRKLAES
LPKREQPPVDEDLVSFEEVKWIKKIEKSEVMKLDKDISKALEMMKEIDRTHKTLPGLDCGSCGSPSCRALAEDIVKGCAT
ENDCIFILKDKIKALAEELNDLAGKKPPVIK
>Mature_430_residues
TYFHSVTLDKDRCRGCTNCIKRCPTEAIRVREGKARIINERCIDCGECIRVCPYHAKLAVTDSLEIMKNFRYKIALPAPS
LYGQFKDLSINQILSALLDIGFDEVFEVAYAAEIVSKFTREALQKGNLKKPVISSACPAVVRLIQIRFPSLIDHLLNICS
PMDTAAIIAKKQAVKKTGLKEEEIGVFFISPCAAKVTSIRNPVGLEKSKIDGAFSMKEIYGKILEKAKTTVVRDLSKATM
IGVGWANSGGEAFGTFTENSIYVDGIHNVIDVLEEIELGKLSDLDFFEGLACIGGCIGGPLTVENPFVAKNRIRKLAESL
PKREQPPVDEDLVSFEEVKWIKKIEKSEVMKLDKDISKALEMMKEIDRTHKTLPGLDCGSCGSPSCRALAEDIVKGCATE
NDCIFILKDKIKALAEELNDLAGKKPPVIK

Specific function: May be involved in hydrogen uptake for the reduction of sulfate to hydrogen sulfide in an electron transport chain. Cytochrome c3 is likely to be the physiological electron carrier for the enzyme [H]

COG id: COG4624

COG function: function code R; Iron only hydrogenase large subunit, C-terminal domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR009016
- InterPro:   IPR004108
- InterPro:   IPR013352 [H]

Pfam domain/function: PF02906 Fe_hyd_lg_C; PF00037 Fer4 [H]

EC number: =1.12.7.2 [H]

Molecular weight: Translated: 47568; Mature: 47437

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
4.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYFHSVTLDKDRCRGCTNCIKRCPTEAIRVREGKARIINERCIDCGECIRVCPYHAKLA
CCEEEEECCCHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHCCHHHHHHHCCCCCEEE
VTDSLEIMKNFRYKIALPAPSLYGQFKDLSINQILSALLDIGFDEVFEVAYAAEIVSKFT
EHHHHHHHHCCCEEEEECCHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
REALQKGNLKKPVISSACPAVVRLIQIRFPSLIDHLLNICSPMDTAAIIAKKQAVKKTGL
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
KEEEIGVFFISPCAAKVTSIRNPVGLEKSKIDGAFSMKEIYGKILEKAKTTVVRDLSKAT
CHHHCCEEEECHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MIGVGWANSGGEAFGTFTENSIYVDGIHNVIDVLEEIELGKLSDLDFFEGLACIGGCIGG
EEEEEECCCCCCEEEEECCCCEEECHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCC
PLTVENPFVAKNRIRKLAESLPKREQPPVDEDLVSFEEVKWIKKIEKSEVMKLDKDISKA
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEMMKEIDRTHKTLPGLDCGSCGSPSCRALAEDIVKGCATENDCIFILKDKIKALAEELN
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHH
DLAGKKPPVIK
HHCCCCCCCCC
>Mature Secondary Structure 
TYFHSVTLDKDRCRGCTNCIKRCPTEAIRVREGKARIINERCIDCGECIRVCPYHAKLA
CEEEEECCCHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHCCHHHHHHHCCCCCEEE
VTDSLEIMKNFRYKIALPAPSLYGQFKDLSINQILSALLDIGFDEVFEVAYAAEIVSKFT
EHHHHHHHHCCCEEEEECCHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
REALQKGNLKKPVISSACPAVVRLIQIRFPSLIDHLLNICSPMDTAAIIAKKQAVKKTGL
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
KEEEIGVFFISPCAAKVTSIRNPVGLEKSKIDGAFSMKEIYGKILEKAKTTVVRDLSKAT
CHHHCCEEEECHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MIGVGWANSGGEAFGTFTENSIYVDGIHNVIDVLEEIELGKLSDLDFFEGLACIGGCIGG
EEEEEECCCCCCEEEEECCCCEEECHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCC
PLTVENPFVAKNRIRKLAESLPKREQPPVDEDLVSFEEVKWIKKIEKSEVMKLDKDISKA
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEMMKEIDRTHKTLPGLDCGSCGSPSCRALAEDIVKGCATENDCIFILKDKIKALAEELN
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHH
DLAGKKPPVIK
HHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3888621; 3322275; 11456963; 10368269; 11456758 [H]