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The map label for this gene is PolA [H]

Identifier: 20807355

GI number: 20807355

Start: 882689

End: 885304

Strand: Direct

Name: PolA [H]

Synonym: TTE0874

Alternate gene names: 20807355

Gene position: 882689-885304 (Clockwise)

Preceding gene: 20807354

Following gene: 20807356

Centisome position: 32.82

GC content: 37.73

Gene sequence:

>2616_bases
ATGGCAAAGTTTCTGTTAATTGATGGTAGCAGTCTGATGTACAGGGCTTATTATGCCTTACCGATGCTTACTACCAGTGA
GGGATTACATACAAATGCATTGTACGGCTTTACTATGATGTTAATAAAATTGCTGGAGGAAGAAAAACCTGACTATGTGG
CAATCGCTTTTGACAAAAAAGCTCCGACTTTCAGGCATAAAGAGTTTAAGGAGTACAAAGCCACCAGACAGGCTATGCCA
GAAGAACTTTCTGAACAGGTAGAGCTTTTAAAAGAAGTAATTGATGGCTTTAACATAAAGACTTTAGAATTGGAAGGATA
TGAAGCAGATGACATAATAGGAACAGTTTCAAAGATGGCAGAGGAAAAAGGGATGGAAGTGGTAATTGTCACGGGAGACA
GAGACGCTCTCCAACTTATTTCCCCCAGGGTAAAGGTGAAAATATCTAAAAAGGGCATAACGCAGATGGAAGAGTTTGAT
GAAAAAGCGGTATTAGAAAGGTATGAAATAACTCCGCAGCAGTTTGTGGATTTAAAGGGATTGATGGGGGACAAGTCGGA
CAATATTCCTGGAATACCTAATATAGGGGAAAAGACTGCTATCAAGCTTTTGAAGGAATTTGGCTCGGTGGAAAATCTTC
TTGAAAATATATCAAAATTGTCAGGAAAGATTAAAGAGACAATAGAAGCAAATAGAGAACTGGCTGTCATGAGCAAAAGA
TTAGCCACTATAAATAGGAATATTCCTCTTGAGATTGACTTTGAAGAGTATAGATTAAAAGAGTTTAATTACAAAAAATT
ATGGGACCTTTTTAACAAGCTTGAATTTTATAGCCTTCTAGACAGCATCAAAAAGGAAGGAGAAGAGGTCATTCCAACTG
TTTCAATAAGAAAGTGGAAAAAAGTTGACATTAGAGAAGTGCCAAGGCTTTTAGAAAGTGCAAAAAATGTTGCCTTTTAT
CCTTTGATCTATGAGGGGGATGTAAAAAAGATAGCATTTTCTTTTGATGGTACTACCCTTTATTCAGATGTTGAAAGGTT
AGAAGACTTAAGAAAAATATTTGAAAGAGAAGATGCTGAATTTGTTTCCCATGAGATAAAAGACTTCCTAGTGAAACTTT
CTTACAAAGGCATAGAATGTAAGAGTAAATATCTGGATACTGCTATAATGGCTTATCTTCTCAACCCTTCTGAGTCCAAT
TACGATTTGGAGAGGGTATTGAAAAGATACCTTAAAGTGGATATATTGTCCCATGAGGAAGTATTTGGGAAAGGAAAAGA
CAGGAGAAGGTATGAAGAAGTAAGCGAAGAAGTAATGGCAGAGTACATATGTGGAAGGTGCAGCCACTTATTTGAGTTAA
AAGATAAGCTTATGGCTTTTATTGAAGAGATGGACATGAAGAAGCTTTTGCTGGAAATAGAGATGCCTCTTGTGGAAGTT
TTAAAATCAATGGAAGTGGATGGATTTACACTGGACAGAAATGTTTTAAAGGAGCTTTCCGAAAAGATAGATGAGAGGAT
TTCTGAAATATTGGATAAAATTTACAAAGAAGCAGGATACCAGTTCAATGTAAATTCCCCAAAGCAGTTGGGAGAATTTC
TCTTTGAAAAGCTCAACCTACCCGTAATTAAAAAGACAAAGACAGGTTACTCGACTGATTCCGAAGTCTTAGAGCAGCTT
ACAGCTTATAATGACATTGTAAGTGAGATAATAGAGTACAGACAGCTTACCAAGCTCAAGTCTACTTACATAGAGGGTTT
TTTGCCCTTGATGGACGAAAATGACAGAGTGCATTCTAATTTCAAACAGATGGTGGCGGCGACTGGCAGGATAAGCAGCA
CAGAGCCTAATCTTCAGAATATACCTGTGAGGGAGGAGTTTGGAAGACAGATAAGGAGGGCTTTTATACCGAGGACAAAA
GACGGCTATATCGTCTCTGCAGACTATTCACAGATTGAATTGAGGGTTTTGGCACATCTTTCTGAAGATGAAAAGTTGAT
AGAAGCTTTTATGAACAATGAAGACATACACTTGAGGACGGCTTCCGAAGTCTTTAAAGTGCCTCCTCAAAATGTCACTC
CAGAGATGAGAAGAGCAGCTAAAGTAGTAAATTTTGGGATAATATATGGAATAAGCGATTATGGCTTGGCGAAAGACTTG
AAGATTTCGAGGAAAGAAGCTAAGGAATATATTGACAATTATTTTGAAAAGTATAAGGGAGTAAAAGAGTATATAGAAAA
GGTGATTCGATTTGCCAAGGAAAATGGATATGTGACTACTATAATGAACAGGAGGAGGTACATTCCTGAAATAAATTCGA
GGAATTTCACACAGCGCTCACAAGCGGAAAGGCTGGCTATGAATACGCCAATCCAAGGCAGTGCTGCCGACATAATAAAA
ATGGCTATGGTAAAAGTTTATCAAGAATTTAAGAGGTTGAATTTAAAGTCCAGGCTCATATTGCAGGTCCATGACGAGCT
TGTTGTTGATACTTATAAGGACGAGCTGGAGTTAGTGAAAGAAATACTCAAAGAAAACATGGAGAATGTGATAAAGCTGA
AAGTTCCGCTGGTTGTTGAAATTGGGGTGGGTCCTAACTGGTTTTTGGCTAAGTGA

Upstream 100 bases:

>100_bases
AGGGTATATTTATTTTAAGAGAAGATGACAATTTACATCTTCTTTTTTTTGCGGTATATTAAAATACATAAAGGAGAAAA
AATTAAACGGGAGTGGTTTA

Downstream 100 bases:

>100_bases
GGTGGAAATATGAGAGTAGTTGGGCTCACGGGAGGCATCGGTTCAGGGAAAAGCACGGTGTCTGGAATTTTGGCAAAACT
CGGGGCTAAGATAATAGATG

Product: DNA polymerase I

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 871; Mature: 870

Protein sequence:

>871_residues
MAKFLLIDGSSLMYRAYYALPMLTTSEGLHTNALYGFTMMLIKLLEEEKPDYVAIAFDKKAPTFRHKEFKEYKATRQAMP
EELSEQVELLKEVIDGFNIKTLELEGYEADDIIGTVSKMAEEKGMEVVIVTGDRDALQLISPRVKVKISKKGITQMEEFD
EKAVLERYEITPQQFVDLKGLMGDKSDNIPGIPNIGEKTAIKLLKEFGSVENLLENISKLSGKIKETIEANRELAVMSKR
LATINRNIPLEIDFEEYRLKEFNYKKLWDLFNKLEFYSLLDSIKKEGEEVIPTVSIRKWKKVDIREVPRLLESAKNVAFY
PLIYEGDVKKIAFSFDGTTLYSDVERLEDLRKIFEREDAEFVSHEIKDFLVKLSYKGIECKSKYLDTAIMAYLLNPSESN
YDLERVLKRYLKVDILSHEEVFGKGKDRRRYEEVSEEVMAEYICGRCSHLFELKDKLMAFIEEMDMKKLLLEIEMPLVEV
LKSMEVDGFTLDRNVLKELSEKIDERISEILDKIYKEAGYQFNVNSPKQLGEFLFEKLNLPVIKKTKTGYSTDSEVLEQL
TAYNDIVSEIIEYRQLTKLKSTYIEGFLPLMDENDRVHSNFKQMVAATGRISSTEPNLQNIPVREEFGRQIRRAFIPRTK
DGYIVSADYSQIELRVLAHLSEDEKLIEAFMNNEDIHLRTASEVFKVPPQNVTPEMRRAAKVVNFGIIYGISDYGLAKDL
KISRKEAKEYIDNYFEKYKGVKEYIEKVIRFAKENGYVTTIMNRRRYIPEINSRNFTQRSQAERLAMNTPIQGSAADIIK
MAMVKVYQEFKRLNLKSRLILQVHDELVVDTYKDELELVKEILKENMENVIKLKVPLVVEIGVGPNWFLAK

Sequences:

>Translated_871_residues
MAKFLLIDGSSLMYRAYYALPMLTTSEGLHTNALYGFTMMLIKLLEEEKPDYVAIAFDKKAPTFRHKEFKEYKATRQAMP
EELSEQVELLKEVIDGFNIKTLELEGYEADDIIGTVSKMAEEKGMEVVIVTGDRDALQLISPRVKVKISKKGITQMEEFD
EKAVLERYEITPQQFVDLKGLMGDKSDNIPGIPNIGEKTAIKLLKEFGSVENLLENISKLSGKIKETIEANRELAVMSKR
LATINRNIPLEIDFEEYRLKEFNYKKLWDLFNKLEFYSLLDSIKKEGEEVIPTVSIRKWKKVDIREVPRLLESAKNVAFY
PLIYEGDVKKIAFSFDGTTLYSDVERLEDLRKIFEREDAEFVSHEIKDFLVKLSYKGIECKSKYLDTAIMAYLLNPSESN
YDLERVLKRYLKVDILSHEEVFGKGKDRRRYEEVSEEVMAEYICGRCSHLFELKDKLMAFIEEMDMKKLLLEIEMPLVEV
LKSMEVDGFTLDRNVLKELSEKIDERISEILDKIYKEAGYQFNVNSPKQLGEFLFEKLNLPVIKKTKTGYSTDSEVLEQL
TAYNDIVSEIIEYRQLTKLKSTYIEGFLPLMDENDRVHSNFKQMVAATGRISSTEPNLQNIPVREEFGRQIRRAFIPRTK
DGYIVSADYSQIELRVLAHLSEDEKLIEAFMNNEDIHLRTASEVFKVPPQNVTPEMRRAAKVVNFGIIYGISDYGLAKDL
KISRKEAKEYIDNYFEKYKGVKEYIEKVIRFAKENGYVTTIMNRRRYIPEINSRNFTQRSQAERLAMNTPIQGSAADIIK
MAMVKVYQEFKRLNLKSRLILQVHDELVVDTYKDELELVKEILKENMENVIKLKVPLVVEIGVGPNWFLAK
>Mature_870_residues
AKFLLIDGSSLMYRAYYALPMLTTSEGLHTNALYGFTMMLIKLLEEEKPDYVAIAFDKKAPTFRHKEFKEYKATRQAMPE
ELSEQVELLKEVIDGFNIKTLELEGYEADDIIGTVSKMAEEKGMEVVIVTGDRDALQLISPRVKVKISKKGITQMEEFDE
KAVLERYEITPQQFVDLKGLMGDKSDNIPGIPNIGEKTAIKLLKEFGSVENLLENISKLSGKIKETIEANRELAVMSKRL
ATINRNIPLEIDFEEYRLKEFNYKKLWDLFNKLEFYSLLDSIKKEGEEVIPTVSIRKWKKVDIREVPRLLESAKNVAFYP
LIYEGDVKKIAFSFDGTTLYSDVERLEDLRKIFEREDAEFVSHEIKDFLVKLSYKGIECKSKYLDTAIMAYLLNPSESNY
DLERVLKRYLKVDILSHEEVFGKGKDRRRYEEVSEEVMAEYICGRCSHLFELKDKLMAFIEEMDMKKLLLEIEMPLVEVL
KSMEVDGFTLDRNVLKELSEKIDERISEILDKIYKEAGYQFNVNSPKQLGEFLFEKLNLPVIKKTKTGYSTDSEVLEQLT
AYNDIVSEIIEYRQLTKLKSTYIEGFLPLMDENDRVHSNFKQMVAATGRISSTEPNLQNIPVREEFGRQIRRAFIPRTKD
GYIVSADYSQIELRVLAHLSEDEKLIEAFMNNEDIHLRTASEVFKVPPQNVTPEMRRAAKVVNFGIIYGISDYGLAKDLK
ISRKEAKEYIDNYFEKYKGVKEYIEKVIRFAKENGYVTTIMNRRRYIPEINSRNFTQRSQAERLAMNTPIQGSAADIIKM
AMVKVYQEFKRLNLKSRLILQVHDELVVDTYKDELELVKEILKENMENVIKLKVPLVVEIGVGPNWFLAK

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity [H]

COG id: COG0749

COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Homo sapiens, GI112420958, Length=515, Percent_Identity=29.3203883495146, Blast_Score=235, Evalue=1e-61,
Organism=Homo sapiens, GI139394648, Length=646, Percent_Identity=25.6965944272446, Blast_Score=220, Evalue=6e-57,
Organism=Escherichia coli, GI1790294, Length=923, Percent_Identity=39.3282773564464, Blast_Score=596, Evalue=1e-171,
Organism=Escherichia coli, GI226510970, Length=221, Percent_Identity=28.0542986425339, Blast_Score=120, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI86563272, Length=419, Percent_Identity=29.3556085918854, Blast_Score=180, Evalue=2e-45,
Organism=Drosophila melanogaster, GI17933644, Length=584, Percent_Identity=30.4794520547945, Blast_Score=252, Evalue=7e-67,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR003583
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 101135; Mature: 101004

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00447 DNA_POLYMERASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKFLLIDGSSLMYRAYYALPMLTTSEGLHTNALYGFTMMLIKLLEEEKPDYVAIAFDKK
CCEEEEECCCHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
APTFRHKEFKEYKATRQAMPEELSEQVELLKEVIDGFNIKTLELEGYEADDIIGTVSKMA
CCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHH
EEKGMEVVIVTGDRDALQLISPRVKVKISKKGITQMEEFDEKAVLERYEITPQQFVDLKG
HHCCCEEEEEECCCHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
LMGDKSDNIPGIPNIGEKTAIKLLKEFGSVENLLENISKLSGKIKETIEANRELAVMSKR
HCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LATINRNIPLEIDFEEYRLKEFNYKKLWDLFNKLEFYSLLDSIKKEGEEVIPTVSIRKWK
HHHHCCCCCEEECHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHC
KVDIREVPRLLESAKNVAFYPLIYEGDVKKIAFSFDGTTLYSDVERLEDLRKIFEREDAE
CCCHHHHHHHHHHCCCCEEEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCHHH
FVSHEIKDFLVKLSYKGIECKSKYLDTAIMAYLLNPSESNYDLERVLKRYLKVDILSHEE
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHH
VFGKGKDRRRYEEVSEEVMAEYICGRCSHLFELKDKLMAFIEEMDMKKLLLEIEMPLVEV
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKSMEVDGFTLDRNVLKELSEKIDERISEILDKIYKEAGYQFNVNSPKQLGEFLFEKLNL
HHHCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHCCC
PVIKKTKTGYSTDSEVLEQLTAYNDIVSEIIEYRQLTKLKSTYIEGFLPLMDENDRVHSN
CEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
FKQMVAATGRISSTEPNLQNIPVREEFGRQIRRAFIPRTKDGYIVSADYSQIELRVLAHL
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHC
SEDEKLIEAFMNNEDIHLRTASEVFKVPPQNVTPEMRRAAKVVNFGIIYGISDYGLAKDL
CCHHHHHHHHHCCCCEEEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHEEECCCCCCCHHH
KISRKEAKEYIDNYFEKYKGVKEYIEKVIRFAKENGYVTTIMNRRRYIPEINSRNFTQRS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHCCCCCCCCCCCCCHHHH
QAERLAMNTPIQGSAADIIKMAMVKVYQEFKRLNLKSRLILQVHDELVVDTYKDELELVK
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EILKENMENVIKLKVPLVVEIGVGPNWFLAK
HHHHHHHHHHEEEEECEEEEECCCCCCCCCC
>Mature Secondary Structure 
AKFLLIDGSSLMYRAYYALPMLTTSEGLHTNALYGFTMMLIKLLEEEKPDYVAIAFDKK
CEEEEECCCHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
APTFRHKEFKEYKATRQAMPEELSEQVELLKEVIDGFNIKTLELEGYEADDIIGTVSKMA
CCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHH
EEKGMEVVIVTGDRDALQLISPRVKVKISKKGITQMEEFDEKAVLERYEITPQQFVDLKG
HHCCCEEEEEECCCHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
LMGDKSDNIPGIPNIGEKTAIKLLKEFGSVENLLENISKLSGKIKETIEANRELAVMSKR
HCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LATINRNIPLEIDFEEYRLKEFNYKKLWDLFNKLEFYSLLDSIKKEGEEVIPTVSIRKWK
HHHHCCCCCEEECHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHC
KVDIREVPRLLESAKNVAFYPLIYEGDVKKIAFSFDGTTLYSDVERLEDLRKIFEREDAE
CCCHHHHHHHHHHCCCCEEEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCHHH
FVSHEIKDFLVKLSYKGIECKSKYLDTAIMAYLLNPSESNYDLERVLKRYLKVDILSHEE
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHH
VFGKGKDRRRYEEVSEEVMAEYICGRCSHLFELKDKLMAFIEEMDMKKLLLEIEMPLVEV
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKSMEVDGFTLDRNVLKELSEKIDERISEILDKIYKEAGYQFNVNSPKQLGEFLFEKLNL
HHHCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHCCC
PVIKKTKTGYSTDSEVLEQLTAYNDIVSEIIEYRQLTKLKSTYIEGFLPLMDENDRVHSN
CEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
FKQMVAATGRISSTEPNLQNIPVREEFGRQIRRAFIPRTKDGYIVSADYSQIELRVLAHL
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHC
SEDEKLIEAFMNNEDIHLRTASEVFKVPPQNVTPEMRRAAKVVNFGIIYGISDYGLAKDL
CCHHHHHHHHHCCCCEEEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHEEECCCCCCCHHH
KISRKEAKEYIDNYFEKYKGVKEYIEKVIRFAKENGYVTTIMNRRRYIPEINSRNFTQRS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHCCCCCCCCCCCCCHHHH
QAERLAMNTPIQGSAADIIKMAMVKVYQEFKRLNLKSRLILQVHDELVVDTYKDELELVK
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EILKENMENVIKLKVPLVVEIGVGPNWFLAK
HHHHHHHHHHEEEEECEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]