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The map label for this gene is iadA [H]

Identifier: 20807319

GI number: 20807319

Start: 845348

End: 846526

Strand: Reverse

Name: iadA [H]

Synonym: TTE0837

Alternate gene names: 20807319

Gene position: 846526-845348 (Counterclockwise)

Preceding gene: 20807322

Following gene: 20807318

Centisome position: 31.48

GC content: 40.63

Gene sequence:

>1179_bases
ATGTTTCTTTTGCTAAAAGGATTAAACGTCTACTCCCCAGAACCAATAGGCAAAAAAGACATACTCATATGTTGCGAGAA
AGTCATAAAAATAGCCGATGAAATAAAACCTTTTAAAGAATTTGGAGATGTAGAAATAGTAGAAGAAGTAGAAGGCCTAA
TAGCTGTACCCGGCTTTATTGACCAGCACGTTCACATAGCAGGGGGAGGCGGAGAAGGTGGTCCTTCAACCAGAACTCCA
GAAATAACTCTATCAGATATAACAAAAGGCGGAATAACTACTGTGGTAGGGCTTTTGGGGACAGACGGAATAACGCGTGG
AATGGCTTCGCTTCTCGCAAAAGCAAGAGCACTAGAACAGGAGGGAATAACAACTTATATTTACACAGGAGCTTACGAGA
TACCTACGCGCACCCTCACAGGAAGTGTAAGGTCAGACCTCGTCTTGATTGACAAAGTCATCGGTACAGGTGAAATTGCG
ATTTCGGACCACAGGTCTGCACAGCCTACAGTAGAAGATTTAACAAAACTAGCAGCAGAAGCCAGAGTTGGAGGACTTCT
AGGTGGCAAACCCGGAATAGTGCACCTTCACGTGGGAGACGGTATAAGAGGCCTTTCTCCTATTTTTGAAATTGTAGAAA
AAACCGAAATACCTATTACCCAGTTTGTTCCAACTCACATAAACAGGGCATCTCATTTATTTGAACATGGCTTGAAATTT
ATAGAAATGGGAGGAACGATAGACCTTACCTCTGACATAAAACCTGACCCCCATTCGAAAACAGCTTTGACCCCAAAAGA
GGCGATTAAAAAAGTCCTGGAAAACAAGCTTCCAATAGAAAAAGTCACAATGAGCTCTGATAGCAACGGCAGCATACCTG
TGTTTGACGAAAACAAAAAACTGGTAAAAGTAATGGTAGGGAGCACAGATACACTCTACAGAGATTTAAGAGAAGCAATT
ATAGAAGGCATTGTCCCCCTTGAAAAAGGGATAAAAATAATAACAGAAAATGTGACAAAAGTATTGAACTTATACCCCAG
AAAAGGATGCATTTCCGAAAAATCTGACGCTGACATAGTCATACTGGACGAAGATTTAAATATCCATTCAGTCATAGCGA
GAGGCAAATTTATGATAAGAAAAGGAAAAATTGTAAAGAAGGGCTTTTTCGAAGAGTAA

Upstream 100 bases:

>100_bases
CGGGCTCTGGCAGGTTCCCGGCCACTTCTCTAATTTTTGCCACCGGTCTGTTATAATATTGATATACGGATGGAAAACTA
ATATGGAAAGGAGGATTATT

Downstream 100 bases:

>100_bases
AAGGAAAAGCAGCATTTTGCGCAAACTCAAGCGCAAAATGCTGCTAATTTTCTTAAAAGGTAGAAAGGTATTTCATCTCC
ATCCCACAAAATTTTATTCA

Product: isoaspartyl dipeptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MFLLLKGLNVYSPEPIGKKDILICCEKVIKIADEIKPFKEFGDVEIVEEVEGLIAVPGFIDQHVHIAGGGGEGGPSTRTP
EITLSDITKGGITTVVGLLGTDGITRGMASLLAKARALEQEGITTYIYTGAYEIPTRTLTGSVRSDLVLIDKVIGTGEIA
ISDHRSAQPTVEDLTKLAAEARVGGLLGGKPGIVHLHVGDGIRGLSPIFEIVEKTEIPITQFVPTHINRASHLFEHGLKF
IEMGGTIDLTSDIKPDPHSKTALTPKEAIKKVLENKLPIEKVTMSSDSNGSIPVFDENKKLVKVMVGSTDTLYRDLREAI
IEGIVPLEKGIKIITENVTKVLNLYPRKGCISEKSDADIVILDEDLNIHSVIARGKFMIRKGKIVKKGFFEE

Sequences:

>Translated_392_residues
MFLLLKGLNVYSPEPIGKKDILICCEKVIKIADEIKPFKEFGDVEIVEEVEGLIAVPGFIDQHVHIAGGGGEGGPSTRTP
EITLSDITKGGITTVVGLLGTDGITRGMASLLAKARALEQEGITTYIYTGAYEIPTRTLTGSVRSDLVLIDKVIGTGEIA
ISDHRSAQPTVEDLTKLAAEARVGGLLGGKPGIVHLHVGDGIRGLSPIFEIVEKTEIPITQFVPTHINRASHLFEHGLKF
IEMGGTIDLTSDIKPDPHSKTALTPKEAIKKVLENKLPIEKVTMSSDSNGSIPVFDENKKLVKVMVGSTDTLYRDLREAI
IEGIVPLEKGIKIITENVTKVLNLYPRKGCISEKSDADIVILDEDLNIHSVIARGKFMIRKGKIVKKGFFEE
>Mature_392_residues
MFLLLKGLNVYSPEPIGKKDILICCEKVIKIADEIKPFKEFGDVEIVEEVEGLIAVPGFIDQHVHIAGGGGEGGPSTRTP
EITLSDITKGGITTVVGLLGTDGITRGMASLLAKARALEQEGITTYIYTGAYEIPTRTLTGSVRSDLVLIDKVIGTGEIA
ISDHRSAQPTVEDLTKLAAEARVGGLLGGKPGIVHLHVGDGIRGLSPIFEIVEKTEIPITQFVPTHINRASHLFEHGLKF
IEMGGTIDLTSDIKPDPHSKTALTPKEAIKKVLENKLPIEKVTMSSDSNGSIPVFDENKKLVKVMVGSTDTLYRDLREAI
IEGIVPLEKGIKIITENVTKVLNLYPRKGCISEKSDADIVILDEDLNIHSVIARGKFMIRKGKIVKKGFFEE

Specific function: Catalyzes the hydrolytic cleavage of a subset of L- isoaspartyl (L-beta-aspartyl) dipeptides. Used to degrade proteins damaged by L-isoaspartyl residues formation. The best substrate for the enzyme reported thus far is iso-Asp-Leu [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M38 family [H]

Homologues:

Organism=Escherichia coli, GI1790784, Length=390, Percent_Identity=45.8974358974359, Blast_Score=341, Evalue=5e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR011059
- InterPro:   IPR010229 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: 3.4.19.- [C]

Molecular weight: Translated: 42456; Mature: 42456

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLLLKGLNVYSPEPIGKKDILICCEKVIKIADEIKPFKEFGDVEIVEEVEGLIAVPGFI
CEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCEEECCCCC
DQHVHIAGGGGEGGPSTRTPEITLSDITKGGITTVVGLLGTDGITRGMASLLAKARALEQ
CCEEEEECCCCCCCCCCCCCEEEHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
EGITTYIYTGAYEIPTRTLTGSVRSDLVLIDKVIGTGEIAISDHRSAQPTVEDLTKLAAE
CCCEEEEEECEEECCCHHCCCCHHHCEEEEEHHHCCCCEEECCCCCCCCCHHHHHHHHHH
ARVGGLLGGKPGIVHLHVGDGIRGLSPIFEIVEKTEIPITQFVPTHINRASHLFEHGLKF
HHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCE
IEMGGTIDLTSDIKPDPHSKTALTPKEAIKKVLENKLPIEKVTMSSDSNGSIPVFDENKK
EECCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEECCCE
LVKVMVGSTDTLYRDLREAIIEGIVPLEKGIKIITENVTKVLNLYPRKGCISEKSDADIV
EEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEE
ILDEDLNIHSVIARGKFMIRKGKIVKKGFFEE
EEECCCCHHHHHHCCEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MFLLLKGLNVYSPEPIGKKDILICCEKVIKIADEIKPFKEFGDVEIVEEVEGLIAVPGFI
CEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCEEECCCCC
DQHVHIAGGGGEGGPSTRTPEITLSDITKGGITTVVGLLGTDGITRGMASLLAKARALEQ
CCEEEEECCCCCCCCCCCCCEEEHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
EGITTYIYTGAYEIPTRTLTGSVRSDLVLIDKVIGTGEIAISDHRSAQPTVEDLTKLAAE
CCCEEEEEECEEECCCHHCCCCHHHCEEEEEHHHCCCCEEECCCCCCCCCHHHHHHHHHH
ARVGGLLGGKPGIVHLHVGDGIRGLSPIFEIVEKTEIPITQFVPTHINRASHLFEHGLKF
HHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCE
IEMGGTIDLTSDIKPDPHSKTALTPKEAIKKVLENKLPIEKVTMSSDSNGSIPVFDENKK
EECCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEECCCE
LVKVMVGSTDTLYRDLREAIIEGIVPLEKGIKIITENVTKVLNLYPRKGCISEKSDADIV
EEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEE
ILDEDLNIHSVIARGKFMIRKGKIVKKGFFEE
EEECCCCHHHHHHCCEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7876157; 7610040; 9278503 [H]