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The map label for this gene is FolC [H]

Identifier: 20807265

GI number: 20807265

Start: 782921

End: 784216

Strand: Direct

Name: FolC [H]

Synonym: TTE0783

Alternate gene names: 20807265

Gene position: 782921-784216 (Clockwise)

Preceding gene: 20807264

Following gene: 20807266

Centisome position: 29.11

GC content: 36.42

Gene sequence:

>1296_bases
ATGGGGCAAATGACATATGAAGAGGCTATCCAGTACATTCACAGTACTTACAAGTTCGGCATGAAATTAGGACTTGAAAA
TATAAAAAGGCTTTTAGGATATATGGGGAATCCTGAAAAAAATCTCAAAATTATCCATGTAGCAGGGACCAATGGAAAAG
GGTCCACTTCTTCTTTTATAAGCAGCATTTTAAAAGAAGCTGGCTATAAAGTGGCTCTTTATACTTCCCCTTACCTGGAA
GAGTTTGAAGAGAGGATGAAAATAAATGGAGAAAATATTTCCAGGGAAAAGCTCGTTTACTATGTAGAGTACATAAAGCC
AATAATTGCAAGGATGGTACAGGAAGGATATAATCATCCAACAGAGTTTGAAGTCATCACTGCCATTGCTTTTAAATACT
TTTCCGATGAAGAAGTAGACTTTGTGGTGCTGGAAGTAGGACTGGGGGGGAGATTTGATGCCACAAATGCTATTTCTTCT
TCACTCGTTTCAGTTATAACTTCTATTGATTATGACCATATGGACAAGCTTGGAAATACCTTAGGCGATATAGCCTATGA
AAAAGCAGGGATAATAAAGAAGAAAGGAGTTGTAGTAAGTTTTTACCAGCAAGAAGAGGCCTTAAAAGTGATTAGTGATG
CCTGTGAGGTAAGAGAAGCCTATTTAACTATCCTGGATAAAAATAATATAGTGATAAAAGAGCAAAATTCAGATTTTCAG
GTTTTTGATTATAAAAATTTTAAGGATTTGAAGATAACTCTTCTGGGTACTCATCAGATTTATAATGCTTCTCTTGCGGT
GGAAGTCATTCAAAAACTTAAGAATATTTATGGATATCAGATAAGCGAAGAGGCAATAAAAAGAGGCTTAATGAATGCGA
AGTGGCCTGGGAGATTAGAAGTCATGAAAAAAAGGCCGTACGTAGTAATAGACGGTGCACACAATCCGCAGGGGATGACA
GTACTGAAAGAAAGCCTGAAATTATTTAATTATAGGAGATTAATTTTGGTTATTGGGATGCTTAAAGATAAAGAAGTAGA
TAAAATGCTTAATATCATAACTCCTGTGGCAGATGTAATTATAACTACTACTCCGCTAAGCGATAGAGCCTGTGGGGCAA
AAGACCTTGCCAGCAAAATTTCTAGAAATAGTGTGTTCGCAGTAGACCAAATTGACAAGGCAGTGAATGAAGCACTAAAA
ATGGCAGGTGAAGAGGATATGGTCCTTTTTTGTGGATCTCTTTACATGATAGGCCATGTGAGGAAATTGTTAAGAGGAGT
ACTAAATAAAAAATGA

Upstream 100 bases:

>100_bases
GCTTTATAGAAGAGGGGAGGAGAAAAAAGCAGTTCAAAATAGGCGATGAATATGTAGATGAAGTTTTAATGGGACTATTT
TTATAAACTTAAAATAGGGG

Downstream 100 bases:

>100_bases
CTTTAATATAATGAAAGGTGTAGAGCCCGTGAAGAGAAGGTGGTTGTTATGGAAATTACAGGTAACACATTGCTGCAGGA
AATAGATAAAGCACTAAAGG

Product: folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MGQMTYEEAIQYIHSTYKFGMKLGLENIKRLLGYMGNPEKNLKIIHVAGTNGKGSTSSFISSILKEAGYKVALYTSPYLE
EFEERMKINGENISREKLVYYVEYIKPIIARMVQEGYNHPTEFEVITAIAFKYFSDEEVDFVVLEVGLGGRFDATNAISS
SLVSVITSIDYDHMDKLGNTLGDIAYEKAGIIKKKGVVVSFYQQEEALKVISDACEVREAYLTILDKNNIVIKEQNSDFQ
VFDYKNFKDLKITLLGTHQIYNASLAVEVIQKLKNIYGYQISEEAIKRGLMNAKWPGRLEVMKKRPYVVIDGAHNPQGMT
VLKESLKLFNYRRLILVIGMLKDKEVDKMLNIITPVADVIITTTPLSDRACGAKDLASKISRNSVFAVDQIDKAVNEALK
MAGEEDMVLFCGSLYMIGHVRKLLRGVLNKK

Sequences:

>Translated_431_residues
MGQMTYEEAIQYIHSTYKFGMKLGLENIKRLLGYMGNPEKNLKIIHVAGTNGKGSTSSFISSILKEAGYKVALYTSPYLE
EFEERMKINGENISREKLVYYVEYIKPIIARMVQEGYNHPTEFEVITAIAFKYFSDEEVDFVVLEVGLGGRFDATNAISS
SLVSVITSIDYDHMDKLGNTLGDIAYEKAGIIKKKGVVVSFYQQEEALKVISDACEVREAYLTILDKNNIVIKEQNSDFQ
VFDYKNFKDLKITLLGTHQIYNASLAVEVIQKLKNIYGYQISEEAIKRGLMNAKWPGRLEVMKKRPYVVIDGAHNPQGMT
VLKESLKLFNYRRLILVIGMLKDKEVDKMLNIITPVADVIITTTPLSDRACGAKDLASKISRNSVFAVDQIDKAVNEALK
MAGEEDMVLFCGSLYMIGHVRKLLRGVLNKK
>Mature_430_residues
GQMTYEEAIQYIHSTYKFGMKLGLENIKRLLGYMGNPEKNLKIIHVAGTNGKGSTSSFISSILKEAGYKVALYTSPYLEE
FEERMKINGENISREKLVYYVEYIKPIIARMVQEGYNHPTEFEVITAIAFKYFSDEEVDFVVLEVGLGGRFDATNAISSS
LVSVITSIDYDHMDKLGNTLGDIAYEKAGIIKKKGVVVSFYQQEEALKVISDACEVREAYLTILDKNNIVIKEQNSDFQV
FDYKNFKDLKITLLGTHQIYNASLAVEVIQKLKNIYGYQISEEAIKRGLMNAKWPGRLEVMKKRPYVVIDGAHNPQGMTV
LKESLKLFNYRRLILVIGMLKDKEVDKMLNIITPVADVIITTTPLSDRACGAKDLASKISRNSVFAVDQIDKAVNEALKM
AGEEDMVLFCGSLYMIGHVRKLLRGVLNKK

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=302, Percent_Identity=30.794701986755, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI66932984, Length=302, Percent_Identity=30.794701986755, Blast_Score=146, Evalue=4e-35,
Organism=Escherichia coli, GI1788654, Length=417, Percent_Identity=31.4148681055156, Blast_Score=180, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17553150, Length=443, Percent_Identity=27.313769751693, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17553148, Length=443, Percent_Identity=27.313769751693, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI71984923, Length=437, Percent_Identity=27.2311212814645, Blast_Score=162, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6323760, Length=360, Percent_Identity=32.2222222222222, Blast_Score=177, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6324815, Length=390, Percent_Identity=31.025641025641, Blast_Score=159, Evalue=8e-40,
Organism=Saccharomyces cerevisiae, GI6322718, Length=290, Percent_Identity=27.5862068965517, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24641571, Length=293, Percent_Identity=30.7167235494881, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24581568, Length=173, Percent_Identity=33.5260115606936, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 48637; Mature: 48506

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: PS00589 PTS_HPR_SER ; PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQMTYEEAIQYIHSTYKFGMKLGLENIKRLLGYMGNPEKNLKIIHVAGTNGKGSTSSFI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHH
SSILKEAGYKVALYTSPYLEEFEERMKINGENISREKLVYYVEYIKPIIARMVQEGYNHP
HHHHHHCCCEEEEEECHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
TEFEVITAIAFKYFSDEEVDFVVLEVGLGGRFDATNAISSSLVSVITSIDYDHMDKLGNT
CHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHH
LGDIAYEKAGIIKKKGVVVSFYQQEEALKVISDACEVREAYLTILDKNNIVIKEQNSDFQ
HHHHHHHHCCCEEECCEEEEEHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEECCCCEE
VFDYKNFKDLKITLLGTHQIYNASLAVEVIQKLKNIYGYQISEEAIKRGLMNAKWPGRLE
EEECCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCHHH
VMKKRPYVVIDGAHNPQGMTVLKESLKLFNYRRLILVIGMLKDKEVDKMLNIITPVADVI
HHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
ITTTPLSDRACGAKDLASKISRNSVFAVDQIDKAVNEALKMAGEEDMVLFCGSLYMIGHV
EECCCCCCCCCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHH
RKLLRGVLNKK
HHHHHHHHCCC
>Mature Secondary Structure 
GQMTYEEAIQYIHSTYKFGMKLGLENIKRLLGYMGNPEKNLKIIHVAGTNGKGSTSSFI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHH
SSILKEAGYKVALYTSPYLEEFEERMKINGENISREKLVYYVEYIKPIIARMVQEGYNHP
HHHHHHCCCEEEEEECHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
TEFEVITAIAFKYFSDEEVDFVVLEVGLGGRFDATNAISSSLVSVITSIDYDHMDKLGNT
CHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHH
LGDIAYEKAGIIKKKGVVVSFYQQEEALKVISDACEVREAYLTILDKNNIVIKEQNSDFQ
HHHHHHHHCCCEEECCEEEEEHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEECCCCEE
VFDYKNFKDLKITLLGTHQIYNASLAVEVIQKLKNIYGYQISEEAIKRGLMNAKWPGRLE
EEECCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCHHH
VMKKRPYVVIDGAHNPQGMTVLKESLKLFNYRRLILVIGMLKDKEVDKMLNIITPVADVI
HHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
ITTTPLSDRACGAKDLASKISRNSVFAVDQIDKAVNEALKMAGEEDMVLFCGSLYMIGHV
EECCCCCCCCCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHH
RKLLRGVLNKK
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]