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The map label for this gene is AtoA [H]

Identifier: 20807208

GI number: 20807208

Start: 723116

End: 723781

Strand: Direct

Name: AtoA [H]

Synonym: TTE0721

Alternate gene names: 20807208

Gene position: 723116-723781 (Clockwise)

Preceding gene: 20807207

Following gene: 20807209

Centisome position: 26.89

GC content: 43.54

Gene sequence:

>666_bases
ATGATAACTGATCAGGAACTGGCAAAGCAGATAATAGCAAAAAGAATTGCAAAAGAGCTCAAACATGGAGAGCTGGTGAA
TTTGGGAATTGGTCTTCCCACTCTAGTAGCCAATTACATTCCCGAAGGGGTGCACGTCATATTCCAATCAGAGAATGGAA
TTACAGGTATGGGACCTATGCCAGAGCCCGGAAAAGAGGATGTAAATATAATTAATGCCGGCGGGCAGTATGTGACAGTT
ATGCCTGGCGCAGCGTTTTTTGACAGCGCATTTTCCTTTGCACTTATAAGAGGAGGACATGTGGACGTTACCGTGCTGGG
GGCATTGGAGGTAGATGAAGAGGGAAATTTAGCTAACTGGATAATACCTGGAAAGTTAGTTCCTGGCATGGGCGGAGCAA
TGGACCTTGCTACAGGAGCCAAAAGAGTAATTGTGGCTATGCAGCATACTGCTAAAGGGCAGCCAAAGATTTTGAAAAAA
TGCCGCCTTCCTCTCACGGCCAAAGGCAAAGTGAGTCTTATAGTTACAGAGCTTGCAGTGATAGAAGTGACAAAAGAGGG
ACTGCTTCTCAAAGAAATTCACAGGGATACATCTATTGAAGAGGTCAAGTCTTTGACAGAGGCAAATTTAATTATTCCAG
ATGATGTAAAATATATGGATGTGTAA

Upstream 100 bases:

>100_bases
GTTGAGGCAGAAAAAATAGTCAAAGTAGGAGAAATAAAGCCAGAAGATGTCATGACGCCAGGAGTATTAGTGGATTATAT
CGTAAAAGGAGGGGAAGGCG

Downstream 100 bases:

>100_bases
AATTCGAAGAAGGAGGAGGATATGCTTATGATGGGATGCAAATACGGTTCTCATAGAGTGATTGAGCCAAAAGGGGTTTT
ACCTCAAGCCGCTTATAAAA

Product: Acyl-CoA:acetate CoA transferase beta subunit

Products: NA

Alternate protein names: Coat B; Acetoacetyl-CoA:acetate/butyrate:CoA transferase subunit B [H]

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MITDQELAKQIIAKRIAKELKHGELVNLGIGLPTLVANYIPEGVHVIFQSENGITGMGPMPEPGKEDVNIINAGGQYVTV
MPGAAFFDSAFSFALIRGGHVDVTVLGALEVDEEGNLANWIIPGKLVPGMGGAMDLATGAKRVIVAMQHTAKGQPKILKK
CRLPLTAKGKVSLIVTELAVIEVTKEGLLLKEIHRDTSIEEVKSLTEANLIIPDDVKYMDV

Sequences:

>Translated_221_residues
MITDQELAKQIIAKRIAKELKHGELVNLGIGLPTLVANYIPEGVHVIFQSENGITGMGPMPEPGKEDVNIINAGGQYVTV
MPGAAFFDSAFSFALIRGGHVDVTVLGALEVDEEGNLANWIIPGKLVPGMGGAMDLATGAKRVIVAMQHTAKGQPKILKK
CRLPLTAKGKVSLIVTELAVIEVTKEGLLLKEIHRDTSIEEVKSLTEANLIIPDDVKYMDV
>Mature_221_residues
MITDQELAKQIIAKRIAKELKHGELVNLGIGLPTLVANYIPEGVHVIFQSENGITGMGPMPEPGKEDVNIINAGGQYVTV
MPGAAFFDSAFSFALIRGGHVDVTVLGALEVDEEGNLANWIIPGKLVPGMGGAMDLATGAKRVIVAMQHTAKGQPKILKK
CRLPLTAKGKVSLIVTELAVIEVTKEGLLLKEIHRDTSIEEVKSLTEANLIIPDDVKYMDV

Specific function: Involved in solventogenic switch which allows C.acetobutylicum to uptake acid and produce solvents. Acts mainly to detoxify the medium by removing the acetate and butyrate excreted earlier in the fermentation [H]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

Organism=Homo sapiens, GI4557817, Length=207, Percent_Identity=49.7584541062802, Blast_Score=203, Evalue=1e-52,
Organism=Homo sapiens, GI11545841, Length=218, Percent_Identity=46.7889908256881, Blast_Score=195, Evalue=2e-50,
Organism=Escherichia coli, GI1788552, Length=211, Percent_Identity=55.9241706161137, Blast_Score=247, Evalue=5e-67,
Organism=Caenorhabditis elegans, GI17531517, Length=215, Percent_Identity=52.5581395348837, Blast_Score=221, Evalue=2e-58,
Organism=Drosophila melanogaster, GI21358325, Length=217, Percent_Identity=50.6912442396313, Blast_Score=216, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24655794, Length=217, Percent_Identity=50.6912442396313, Blast_Score=216, Evalue=1e-56,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.9 [H]

Molecular weight: Translated: 23670; Mature: 23670

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITDQELAKQIIAKRIAKELKHGELVNLGIGLPTLVANYIPEGVHVIFQSENGITGMGPM
CCCHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCCCEEEEEECCCCCCCCCCC
PEPGKEDVNIINAGGQYVTVMPGAAFFDSAFSFALIRGGHVDVTVLGALEVDEEGNLANW
CCCCCCCCEEEECCCCEEEECCCHHHHHHHHHEEEEECCCEEEEEEEEEEECCCCCEEEE
IIPGKLVPGMGGAMDLATGAKRVIVAMQHTAKGQPKILKKCRLPLTAKGKVSLIVTELAV
EECCCCCCCCCCCHHHHCCCCEEEEEEECCCCCCHHHHHHCCCCCCCCCCEEEEEEEHEE
IEVTKEGLLLKEIHRDTSIEEVKSLTEANLIIPDDVKYMDV
EEECCCCHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEECC
>Mature Secondary Structure
MITDQELAKQIIAKRIAKELKHGELVNLGIGLPTLVANYIPEGVHVIFQSENGITGMGPM
CCCHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCCCEEEEEECCCCCCCCCCC
PEPGKEDVNIINAGGQYVTVMPGAAFFDSAFSFALIRGGHVDVTVLGALEVDEEGNLANW
CCCCCCCCEEEECCCCEEEECCCHHHHHHHHHEEEEECCCEEEEEEEEEEECCCCCEEEE
IIPGKLVPGMGGAMDLATGAKRVIVAMQHTAKGQPKILKKCRLPLTAKGKVSLIVTELAV
EECCCCCCCCCCCHHHHCCCCEEEEEEECCCCCCHHHHHHCCCCCCCCCCEEEEEEEHEE
IEVTKEGLLLKEIHRDTSIEEVKSLTEANLIIPDDVKYMDV
EEECCCCHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2254264; 8226639; 8423010; 11466286; 2383002 [H]