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The map label for this gene is WecB2 [C]

Identifier: 20807152

GI number: 20807152

Start: 663418

End: 664509

Strand: Direct

Name: WecB2 [C]

Synonym: TTE0661

Alternate gene names: 20807152

Gene position: 663418-664509 (Clockwise)

Preceding gene: 20807151

Following gene: 20807153

Centisome position: 24.67

GC content: 34.25

Gene sequence:

>1092_bases
ATGAAAATAGTAACTATTATCGGTACTAGGCCGCAATTTATTAAGGCTGCTACAGTCTCGAGAAAGCTAAAAGATTTCAA
TGATATGAAAGAAATTATTGTACATACAGGACAGCATTATGATGATAATATGAGTGATATATTCTTCAAAGAACTTGAAA
TTCCTCAGCCTGATTACTATCTTGGTATAGGATCAGGTAGTCATGGAGTACAAACAGCTCATATGCTTGAAAAAATTGAA
GATGTTTTAATAAAAGAAAAGCCTGATGTAGTATTAATTTATGGTGATACAAATTCTACACTTGCAGGAACACTCGCTGC
ATCGAAGCTCCAGATTCCTGTTGCTCATGTAGAGGCTGGGCTTCGCTCTTTTAATCGAAAAATGCCAGAAGAAATTAACC
GTATAGTTGCAGATCATGTATCTGATTTACTTTTTGCTCCTACAAAAGTGGCAGTAAATAATTTAATTAATGAAGGGATA
GCTGAAGATAGGATATATTATGTTGGAGATGTGATGTTTGATGCTGCTATATTTTACGGAGAAAAAGCAGGACAGCAGAG
CAAAATATTAGAAACTTTGGATTTAAAACCCAAGGAATACATCCTTTCTACAATCCATAGGGCTTATAATACTGACATTC
CTGAAAGATTGAAAACAATTTTTAATGCGCTTTGTAAAATAGGGAAGGATATCACGATTGTTATGCCACTTCATCCACGT
ACAAAAGCTGCATTATTAAGAGAGGGACTCTATGAGAAAGTATCGGAAAAACTGAAAATAATAGAGCCAGTTGGGTATCT
TGATATGGTTAAATTAGAAAAAAATGCAAAGCTCATAATTACTGATTCTGGAGGAGTGCAAAAAGAAGCATTTTTTTATA
AGGTACTATGTGTTACATTAAGAGAAGAAACAGAATGGGTAGAACTTGTGGATTTGGGATGGAATTATCTTGTACCTCCA
GATTGTGAAAAATTTATTGAAGAAAGTATAAGAAAAGTTTTGAATGCTCCACATGGTTTAGATGCAGAGCCTTATGGAGA
TGGGAAGGCGGCAGAAAAAATAGTTAAGGTGTTAAGAAAATATCTGTTGTAA

Upstream 100 bases:

>100_bases
TTGAGAAAATAGAAAAAGAATTTAATTTATCGAAAATTGTAGAAGAGTTACTATATTTGATGGGTAATAATTAAAATGGA
TTTTGAGGGAGTAAGGAATT

Downstream 100 bases:

>100_bases
GGTAAGATTTAACATAATGAGGTAAGGTGATAATCTTTGAGTGGTAAAAATATTTGGATATTAAATCATTACGCAATTAG
TCCTGACATGGCAGGTGGAA

Product: UDP-N-acetylglucosamine 2-epimerase

Products: UDP-N-acetyl-D-mannosamine

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MKIVTIIGTRPQFIKAATVSRKLKDFNDMKEIIVHTGQHYDDNMSDIFFKELEIPQPDYYLGIGSGSHGVQTAHMLEKIE
DVLIKEKPDVVLIYGDTNSTLAGTLAASKLQIPVAHVEAGLRSFNRKMPEEINRIVADHVSDLLFAPTKVAVNNLINEGI
AEDRIYYVGDVMFDAAIFYGEKAGQQSKILETLDLKPKEYILSTIHRAYNTDIPERLKTIFNALCKIGKDITIVMPLHPR
TKAALLREGLYEKVSEKLKIIEPVGYLDMVKLEKNAKLIITDSGGVQKEAFFYKVLCVTLREETEWVELVDLGWNYLVPP
DCEKFIEESIRKVLNAPHGLDAEPYGDGKAAEKIVKVLRKYLL

Sequences:

>Translated_363_residues
MKIVTIIGTRPQFIKAATVSRKLKDFNDMKEIIVHTGQHYDDNMSDIFFKELEIPQPDYYLGIGSGSHGVQTAHMLEKIE
DVLIKEKPDVVLIYGDTNSTLAGTLAASKLQIPVAHVEAGLRSFNRKMPEEINRIVADHVSDLLFAPTKVAVNNLINEGI
AEDRIYYVGDVMFDAAIFYGEKAGQQSKILETLDLKPKEYILSTIHRAYNTDIPERLKTIFNALCKIGKDITIVMPLHPR
TKAALLREGLYEKVSEKLKIIEPVGYLDMVKLEKNAKLIITDSGGVQKEAFFYKVLCVTLREETEWVELVDLGWNYLVPP
DCEKFIEESIRKVLNAPHGLDAEPYGDGKAAEKIVKVLRKYLL
>Mature_363_residues
MKIVTIIGTRPQFIKAATVSRKLKDFNDMKEIIVHTGQHYDDNMSDIFFKELEIPQPDYYLGIGSGSHGVQTAHMLEKIE
DVLIKEKPDVVLIYGDTNSTLAGTLAASKLQIPVAHVEAGLRSFNRKMPEEINRIVADHVSDLLFAPTKVAVNNLINEGI
AEDRIYYVGDVMFDAAIFYGEKAGQQSKILETLDLKPKEYILSTIHRAYNTDIPERLKTIFNALCKIGKDITIVMPLHPR
TKAALLREGLYEKVSEKLKIIEPVGYLDMVKLEKNAKLIITDSGGVQKEAFFYKVLCVTLREETEWVELVDLGWNYLVPP
DCEKFIEESIRKVLNAPHGLDAEPYGDGKAAEKIVKVLRKYLL

Specific function: Catalyzes The Reversible Epimerization At C-2 Of UDP-N- Acetylglucosamine (UDP-Glcnac) And Thereby Provides Bacteria With UDP-N-Acetylmannosamine (UDP-Mannac), The Activated Donor Of Mannac Residues. Also Involved In Bacteriophage N4 Adsorption. [C]

COG id: COG0381

COG function: function code M; UDP-N-acetylglucosamine 2-epimerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDP-N-acetylglucosamine 2-epimerase family [H]

Homologues:

Organism=Escherichia coli, GI48994967, Length=387, Percent_Identity=34.1085271317829, Blast_Score=157, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003331 [H]

Pfam domain/function: PF02350 Epimerase_2 [H]

EC number: 5.1.3.14

Molecular weight: Translated: 40963; Mature: 40963

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVTIIGTRPQFIKAATVSRKLKDFNDMKEIIVHTGQHYDDNMSDIFFKELEIPQPDYY
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCEE
LGIGSGSHGVQTAHMLEKIEDVLIKEKPDVVLIYGDTNSTLAGTLAASKLQIPVAHVEAG
EEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHCCHHHHHHHH
LRSFNRKMPEEINRIVADHVSDLLFAPTKVAVNNLINEGIAEDRIYYVGDVMFDAAIFYG
HHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHC
EKAGQQSKILETLDLKPKEYILSTIHRAYNTDIPERLKTIFNALCKIGKDITIVMPLHPR
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEECCCC
TKAALLREGLYEKVSEKLKIIEPVGYLDMVKLEKNAKLIITDSGGVQKEAFFYKVLCVTL
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEECCCCEEEEECCCCCCHHHHHHHHHHHHH
REETEWVELVDLGWNYLVPPDCEKFIEESIRKVLNAPHGLDAEPYGDGKAAEKIVKVLRK
HCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
YLL
HCC
>Mature Secondary Structure
MKIVTIIGTRPQFIKAATVSRKLKDFNDMKEIIVHTGQHYDDNMSDIFFKELEIPQPDYY
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCEE
LGIGSGSHGVQTAHMLEKIEDVLIKEKPDVVLIYGDTNSTLAGTLAASKLQIPVAHVEAG
EEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHCCHHHHHHHH
LRSFNRKMPEEINRIVADHVSDLLFAPTKVAVNNLINEGIAEDRIYYVGDVMFDAAIFYG
HHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHC
EKAGQQSKILETLDLKPKEYILSTIHRAYNTDIPERLKTIFNALCKIGKDITIVMPLHPR
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEECCCC
TKAALLREGLYEKVSEKLKIIEPVGYLDMVKLEKNAKLIITDSGGVQKEAFFYKVLCVTL
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEECCCCEEEEECCCCCCHHHHHHHHHHHHH
REETEWVELVDLGWNYLVPPDCEKFIEESIRKVLNAPHGLDAEPYGDGKAAEKIVKVLRK
HCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
YLL
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: UDP-N-acetylglucosamine [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.63 {UDP-N-acetylglucosamine}} 1 {UDP-N-acetylmannosamine}} 0.5 {UDP-N-acetylmannosamine}} [C]

Substrates: UDP-N-acetyl-D-glucosamine

Specific reaction: UDP-N-acetyl-D-glucosamine = UDP-N-acetyl-D-mannosamine

General reaction: Epimerization [C]

Inhibitor: PCMB [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA