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The map label for this gene is NadE [H]

Identifier: 20807133

GI number: 20807133

Start: 643030

End: 644661

Strand: Reverse

Name: NadE [H]

Synonym: TTE0640

Alternate gene names: 20807133

Gene position: 644661-643030 (Counterclockwise)

Preceding gene: 20807134

Following gene: 20807132

Centisome position: 23.97

GC content: 36.15

Gene sequence:

>1632_bases
ATGAAAATTGCACTGGCACAGATAAACCCTGTTGTTGGAGATATAAAACATAACTGTGAAAAAATAGTAAAATATATTGA
AAAGGCAAAAAAAGAAAAGGCGGATTTGGTTGTATTTCCTGAACTGTCGACTGTTGGCTATCCTCCTAAAGACTTTCTTT
TCGTGAAAGACTTCATAAAAACAAATGAAGAGATGATAAATAAAATAATACTGCCGGCAACTGATGAAATTGCCGTAATA
CTAGGTACTGTGAGAAAAGATGAAAATAAAAATTTGTATAATTCTGCCTTTTTTGTTTACAACAGAAAAATAATAGAAAT
ATTTGATAAAACGCTCCTTCCCAATTATGACGTATTTGATGAGAAAAGATATTTTTCTCCTTCATCACAGATAAAAACAG
TTGAATTTAAGGGCGTAAAAGTAGCAGTAAATATATGTGAGGATGCATGGAAAGGCTATGTGTTTGAGCCAAGCGCAAAC
TATTCTGTTGATGTTTTGGAAGAGCAATTTAAACTAAAACCGGATATTTTTATAAACATCTCTGCCTCTCCCTATCATTT
GGGCAAACACAGGACAAGGCTTGGAATAATGAAAGAGAAAACTGATAAATACGGCATTCCTTTTGTGTACGTCAACCAGG
TAGGAGCAAATGATGAACTCATTTTCGATGGAAATAGTTTCGTATTAAATGAAAAAGGAAAAGAGGTAATAAGGCTAAAA
GGATTTGAAGAGGATTTTAAAGTAGTAGAGTTGCAAAATTTGGAGGACTTCGAAGAGCTGCCAGAACTTAAAGAAGATAT
ATCTTGGATATATAAGGCTCTTGTAATTGGAGTTAGGGATTACTTTCATAAATTAGGATTTAAAAAAGCGGTTCTGGGAC
TAAGCGGTGGAATTGATTCTGCAGTAGTGGCAGTAATAGCCTCTGAAGCCCTGGGAAAAGAAAATGTATTGGCAATTTCT
ATGCCTTCAAGGTATTCTTCTGAAGGAAGTGTAAAAGATGCAGAAATCTTGGCCAAAAACTTAGGCATAGACTTTAGAGT
GATTCCTATTGAGCCTGTTTTCAAAAGCTATTTATCTGTCTTCAACAAAGATGGAAATGCTTTAGGAGATGTGGCAGAGG
AAAATGTACAGGCTAGAATACGCGGAAATTATCTCATGTTCTTTGCAAACAGGGAAGGATACGTAGTGCTTACAACAGGC
AATAAGTCAGAGTTGGCAATGGGTTATTGCACTTTGTACGGAGATATGAGTGGAAGTCTAGCAGTGATTGGCGACTTATA
TAAAACTCAAGTATATGAGTTGGCGAGGTATATAAATAGAGAAAAAGAGATAATACCAGTTTCAATAATTAAAAAAGTTC
CTTCGGCAGAGCTAAGGCCAAACCAAAAAGATGAAGACTCTCTTCCCCCTTACAGAATACTGGACCAAGTGTTGAAGCTT
TATCTAGAGGAAAATGCTTCTGTGGAGGAAATAGCAGAAAAAGGCTTTGACAAAGACCTGGTAAGGCACATCATAAATAC
TGTAAATAAAAATGAATACAAGAGAAAACAGGCCCCTCCTGTACTTAGAGTCTCACCTAAAGCCTTTGGCACTGGCAGGA
GAATGCCAATCGTACAAAATTTCAAAGCGTAG

Upstream 100 bases:

>100_bases
TTGAAAAAAAGCTCATTCGCTGGAGATAGAAAGAGATGCTTTTTATTTTAACATGTGCTAAAATATGTTTGTAATACATC
GTACGTAGAGGTGAAAAGAA

Downstream 100 bases:

>100_bases
GGGGAGATAATTTTGTCAAAGCAACTTCAAAGCGATATAGTGCTGACTCTTGTGACAATGATATGGGGTTCTACTTTTAT
AATAGTGAAAAATGCCATTC

Product: NAD synthase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 543; Mature: 543

Protein sequence:

>543_residues
MKIALAQINPVVGDIKHNCEKIVKYIEKAKKEKADLVVFPELSTVGYPPKDFLFVKDFIKTNEEMINKIILPATDEIAVI
LGTVRKDENKNLYNSAFFVYNRKIIEIFDKTLLPNYDVFDEKRYFSPSSQIKTVEFKGVKVAVNICEDAWKGYVFEPSAN
YSVDVLEEQFKLKPDIFINISASPYHLGKHRTRLGIMKEKTDKYGIPFVYVNQVGANDELIFDGNSFVLNEKGKEVIRLK
GFEEDFKVVELQNLEDFEELPELKEDISWIYKALVIGVRDYFHKLGFKKAVLGLSGGIDSAVVAVIASEALGKENVLAIS
MPSRYSSEGSVKDAEILAKNLGIDFRVIPIEPVFKSYLSVFNKDGNALGDVAEENVQARIRGNYLMFFANREGYVVLTTG
NKSELAMGYCTLYGDMSGSLAVIGDLYKTQVYELARYINREKEIIPVSIIKKVPSAELRPNQKDEDSLPPYRILDQVLKL
YLEENASVEEIAEKGFDKDLVRHIINTVNKNEYKRKQAPPVLRVSPKAFGTGRRMPIVQNFKA

Sequences:

>Translated_543_residues
MKIALAQINPVVGDIKHNCEKIVKYIEKAKKEKADLVVFPELSTVGYPPKDFLFVKDFIKTNEEMINKIILPATDEIAVI
LGTVRKDENKNLYNSAFFVYNRKIIEIFDKTLLPNYDVFDEKRYFSPSSQIKTVEFKGVKVAVNICEDAWKGYVFEPSAN
YSVDVLEEQFKLKPDIFINISASPYHLGKHRTRLGIMKEKTDKYGIPFVYVNQVGANDELIFDGNSFVLNEKGKEVIRLK
GFEEDFKVVELQNLEDFEELPELKEDISWIYKALVIGVRDYFHKLGFKKAVLGLSGGIDSAVVAVIASEALGKENVLAIS
MPSRYSSEGSVKDAEILAKNLGIDFRVIPIEPVFKSYLSVFNKDGNALGDVAEENVQARIRGNYLMFFANREGYVVLTTG
NKSELAMGYCTLYGDMSGSLAVIGDLYKTQVYELARYINREKEIIPVSIIKKVPSAELRPNQKDEDSLPPYRILDQVLKL
YLEENASVEEIAEKGFDKDLVRHIINTVNKNEYKRKQAPPVLRVSPKAFGTGRRMPIVQNFKA
>Mature_543_residues
MKIALAQINPVVGDIKHNCEKIVKYIEKAKKEKADLVVFPELSTVGYPPKDFLFVKDFIKTNEEMINKIILPATDEIAVI
LGTVRKDENKNLYNSAFFVYNRKIIEIFDKTLLPNYDVFDEKRYFSPSSQIKTVEFKGVKVAVNICEDAWKGYVFEPSAN
YSVDVLEEQFKLKPDIFINISASPYHLGKHRTRLGIMKEKTDKYGIPFVYVNQVGANDELIFDGNSFVLNEKGKEVIRLK
GFEEDFKVVELQNLEDFEELPELKEDISWIYKALVIGVRDYFHKLGFKKAVLGLSGGIDSAVVAVIASEALGKENVLAIS
MPSRYSSEGSVKDAEILAKNLGIDFRVIPIEPVFKSYLSVFNKDGNALGDVAEENVQARIRGNYLMFFANREGYVVLTTG
NKSELAMGYCTLYGDMSGSLAVIGDLYKTQVYELARYINREKEIIPVSIIKKVPSAELRPNQKDEDSLPPYRILDQVLKL
YLEENASVEEIAEKGFDKDLVRHIINTVNKNEYKRKQAPPVLRVSPKAFGTGRRMPIVQNFKA

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI17538756, Length=663, Percent_Identity=23.5294117647059, Blast_Score=95, Evalue=1e-19,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 61564; Mature: 61564

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIALAQINPVVGDIKHNCEKIVKYIEKAKKEKADLVVFPELSTVGYPPKDFLFVKDFIK
CEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHCCCCCHHHHHHHHHHH
TNEEMINKIILPATDEIAVILGTVRKDENKNLYNSAFFVYNRKIIEIFDKTLLPNYDVFD
CCHHHHHHEECCCCCCEEEEEEECCCCCCCCHHHCEEEEECCHHHHHHHHHHCCCCCCCC
EKRYFSPSSQIKTVEFKGVKVAVNICEDAWKGYVFEPSANYSVDVLEEQFKLKPDIFINI
CCCCCCCCCCEEEEEECCEEEEEEEHHHHHCCEEECCCCCCCHHHHHHHHCCCCCEEEEE
SASPYHLGKHRTRLGIMKEKTDKYGIPFVYVNQVGANDELIFDGNSFVLNEKGKEVIRLK
CCCCEECCCHHHHHCCHHHCCCCCCCCEEEEEECCCCCCEEECCCEEEECCCCCEEEEEC
GFEEDFKVVELQNLEDFEELPELKEDISWIYKALVIGVRDYFHKLGFKKAVLGLSGGIDS
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHH
AVVAVIASEALGKENVLAISMPSRYSSEGSVKDAEILAKNLGIDFRVIPIEPVFKSYLSV
HHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHH
FNKDGNALGDVAEENVQARIRGNYLMFFANREGYVVLTTGNKSELAMGYCTLYGDMSGSL
HCCCCCHHHHHHHHCCEEEEECCEEEEEECCCCEEEEEECCCCCEEEEEEEEEECCCCCC
AVIGDLYKTQVYELARYINREKEIIPVSIIKKVPSAELRPNQKDEDSLPPYRILDQVLKL
HHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
YLEENASVEEIAEKGFDKDLVRHIINTVNKNEYKRKQAPPVLRVSPKAFGTGRRMPIVQN
HHHCCCCHHHHHHCCCCHHHHHHHHHHCCCCHHHHHCCCCEEEECCHHCCCCCCCCCCCC
FKA
CCC
>Mature Secondary Structure
MKIALAQINPVVGDIKHNCEKIVKYIEKAKKEKADLVVFPELSTVGYPPKDFLFVKDFIK
CEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHCCCCCHHHHHHHHHHH
TNEEMINKIILPATDEIAVILGTVRKDENKNLYNSAFFVYNRKIIEIFDKTLLPNYDVFD
CCHHHHHHEECCCCCCEEEEEEECCCCCCCCHHHCEEEEECCHHHHHHHHHHCCCCCCCC
EKRYFSPSSQIKTVEFKGVKVAVNICEDAWKGYVFEPSANYSVDVLEEQFKLKPDIFINI
CCCCCCCCCCEEEEEECCEEEEEEEHHHHHCCEEECCCCCCCHHHHHHHHCCCCCEEEEE
SASPYHLGKHRTRLGIMKEKTDKYGIPFVYVNQVGANDELIFDGNSFVLNEKGKEVIRLK
CCCCEECCCHHHHHCCHHHCCCCCCCCEEEEEECCCCCCEEECCCEEEECCCCCEEEEEC
GFEEDFKVVELQNLEDFEELPELKEDISWIYKALVIGVRDYFHKLGFKKAVLGLSGGIDS
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHH
AVVAVIASEALGKENVLAISMPSRYSSEGSVKDAEILAKNLGIDFRVIPIEPVFKSYLSV
HHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHH
FNKDGNALGDVAEENVQARIRGNYLMFFANREGYVVLTTGNKSELAMGYCTLYGDMSGSL
HCCCCCHHHHHHHHCCEEEEECCEEEEEECCCCEEEEEECCCCCEEEEEEEEEECCCCCC
AVIGDLYKTQVYELARYINREKEIIPVSIIKKVPSAELRPNQKDEDSLPPYRILDQVLKL
HHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
YLEENASVEEIAEKGFDKDLVRHIINTVNKNEYKRKQAPPVLRVSPKAFGTGRRMPIVQN
HHHCCCCHHHHHHCCCCHHHHHHHHHHCCCCHHHHHCCCCEEEECCHHCCCCCCCCCCCC
FKA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]