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Identifier: 20807100

GI number: 20807100

Start: 607685

End: 609685

Strand: Direct

Name: Lig

Synonym: TTE0605

Alternate gene names: 20807100

Gene position: 607685-609685 (Clockwise)

Preceding gene: 20807099

Following gene: 20807101

Centisome position: 22.6

GC content: 42.03

Gene sequence:

>2001_bases
ATGGATAGGGAAGAGGCAAGAAAGAGGATAGAGGAGTTAAGGGAAAAAATAAACTATCACAACTACAGGTATTATGTGTT
GGACCAGCCAGAAATTTCAGACTATGAGTATGATATGCTTATGAGAGAACTCATAGAATTGGAAGAAAAATATCCTGAGT
TTAAGACTCCAGACTCTCCCTCTCAGAGGGTAGGAGGAGAGCCACTTGATGAGTTTGAGCCTTTTACTCACATTGTTCCA
ATGTTGAGCTTGGCCAATGCTTTTACGGCAGAAGAGATTAAGGAATTTGACAGAAGGGTAAAAGAGGCGGTAGGGGAAGT
AGAGTACGTAGTAGAGCCTAAAATTGACGGTTTGTCTGTGGAGCTGGTGTATGAAAATGGCATGTTCACAGTGGGATCTA
CTAGAGGAGACGGAATTGTGGGGGAGAATGTCACTCCGAATTTGAAGACCATAAAGTCCATTCCTTTAAGGCTTAAAGAT
TCTGTGAACCTGGTTGTAAGAGGAGAAGTTTTTATGCCAAAAGCTTCTTTTGCCAAGCTCAACGAAGAGAGAGCCGAAAG
AGGAGAATCCCTTTTTGCAAATCCCAGGAATGCTGCAGCTGGTTCTGTGAGGCAGTTAGACCCTAAAGTCACTGCAAAAA
GGGACCTAGACATTTTCATTTTTAACCTTCAGAGGATTGAAGGAAGAGATTTCAAGACTCATGTGGAAGCCTTAGAATTT
TTGAAAGAACAGGGATTTAAAGTAATTCCTCTTATTAAAAAATGCACTACAATCGAAGAAGTCATCAAAGCTATAGAAGA
GCTAGGGGAGATGAAAGATTCTTTGCCGTACGATATAGACGGCGCAGTGATAAAAGTGAATGAACTTGATAAAAGGGAGA
TTTTGGGGCAGACTGCTAAAGACTACAGATGGGCTATAGCATTTAAATATCCTGCAGAGATGAAGAAAACAAAAATAGTG
GATATAGTGGTACAAGTGGGTAGGACTGGAGCCTTAACTCCTACGGCTGTCCTCGAGCCTGTTGTTATTTCTGGTTCTGT
GGTGAGCAGAGCTACTCTTCACAATGAAGATTACATAAAGGAGAAGGACATACGAATAGGGGATACTGTGCTTGTGCACA
AAGCAGGAGGCATAATTCCAGAGGTAGTGGAAGTGGTTAAAGAAGAGAGAACAGGCGATGAAAAGGCATTTGTCATGCCT
GACAAATGCCCTGAGTGCGGCGCTTTGGCTGTAAGGCTTCCTGGAGAGGCAGTGAGAAGATGTACAGGCCTTAACTGTCC
TGCTCAGATTGCGAGAAGGATAATCCACTTTGCTTCAAAGGATGCTATGGACATAGAAGGATTGGGCCCTGCTATAATAA
GCCAGCTGCTTTCAAAAGGGCTTATACAAAATGTAGCAGACCTTTACTACCTCAAGTACAGGGATTTAATTACTTTGGAG
AGGATGGGAGATAAGTCTGCTAGAAATTTGCTGGAAGCTATTGAAAAGAGCAAGAAAAGAGACTTAGACAGGTTGCTTTT
TGGACTTGGGATAAACTTGATAGGCAGTAAAGCGGCACAGGTGATTGCAGAGCATTTCAAGAGCATGGACAACATAATGA
AAGCAAAATACGAGGATTTTCTTGAACTTCCGGATATTGGACCCAAAATGGCAAGAAGTATAGTTACTTTCTTTTCTGAA
GAGCAAAACAGAAGAGTGGTTGAAAGGTTGAAAGAAGCGGGAGTCAATATGGAGAAGCTCTCCACAGGTAAAGTGAGCAA
TATATTTGAAGGCAAGATTTTTGTGCTGACAGGAGCGCTTAAAAATTACACTAGAGATGAGGCTGCAAGACTTATTGTTG
AGAGAGGGGGCAAAGTTACTAACTCTGTGAGCAAAAAGACCGACTATCTAATTGTGGGTGCTGACCCTGGCTCTAAGCTT
AAAAAAGCACAGGAACTTGGAGTTAAGATAATTAACGAAGAGCAGTTTGAAGCCATGCTAAAAGGCGATATCCAACCGTA
G

Upstream 100 bases:

>100_bases
GGGGAAGAGATTACAGTAGCCTTTCCGAACGTTGGTATAAAAAAGCTTGATTTGAGATTTGCTCCTATTAAGGCAATTTC
TTAAAACCGGAGGGGAATTT

Downstream 100 bases:

>100_bases
ATTTTTGTGGTAAACTATAAGGGTGAAGAAATAAAGAGAAAGGTGTGGTAAAATGGCAATAAGCAAAAGCGAAGTAGAAT
ACATTGCTAAGCTTGCCAGG

Product: NAD-dependent DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 666; Mature: 666

Protein sequence:

>666_residues
MDREEARKRIEELREKINYHNYRYYVLDQPEISDYEYDMLMRELIELEEKYPEFKTPDSPSQRVGGEPLDEFEPFTHIVP
MLSLANAFTAEEIKEFDRRVKEAVGEVEYVVEPKIDGLSVELVYENGMFTVGSTRGDGIVGENVTPNLKTIKSIPLRLKD
SVNLVVRGEVFMPKASFAKLNEERAERGESLFANPRNAAAGSVRQLDPKVTAKRDLDIFIFNLQRIEGRDFKTHVEALEF
LKEQGFKVIPLIKKCTTIEEVIKAIEELGEMKDSLPYDIDGAVIKVNELDKREILGQTAKDYRWAIAFKYPAEMKKTKIV
DIVVQVGRTGALTPTAVLEPVVISGSVVSRATLHNEDYIKEKDIRIGDTVLVHKAGGIIPEVVEVVKEERTGDEKAFVMP
DKCPECGALAVRLPGEAVRRCTGLNCPAQIARRIIHFASKDAMDIEGLGPAIISQLLSKGLIQNVADLYYLKYRDLITLE
RMGDKSARNLLEAIEKSKKRDLDRLLFGLGINLIGSKAAQVIAEHFKSMDNIMKAKYEDFLELPDIGPKMARSIVTFFSE
EQNRRVVERLKEAGVNMEKLSTGKVSNIFEGKIFVLTGALKNYTRDEAARLIVERGGKVTNSVSKKTDYLIVGADPGSKL
KKAQELGVKIINEEQFEAMLKGDIQP

Sequences:

>Translated_666_residues
MDREEARKRIEELREKINYHNYRYYVLDQPEISDYEYDMLMRELIELEEKYPEFKTPDSPSQRVGGEPLDEFEPFTHIVP
MLSLANAFTAEEIKEFDRRVKEAVGEVEYVVEPKIDGLSVELVYENGMFTVGSTRGDGIVGENVTPNLKTIKSIPLRLKD
SVNLVVRGEVFMPKASFAKLNEERAERGESLFANPRNAAAGSVRQLDPKVTAKRDLDIFIFNLQRIEGRDFKTHVEALEF
LKEQGFKVIPLIKKCTTIEEVIKAIEELGEMKDSLPYDIDGAVIKVNELDKREILGQTAKDYRWAIAFKYPAEMKKTKIV
DIVVQVGRTGALTPTAVLEPVVISGSVVSRATLHNEDYIKEKDIRIGDTVLVHKAGGIIPEVVEVVKEERTGDEKAFVMP
DKCPECGALAVRLPGEAVRRCTGLNCPAQIARRIIHFASKDAMDIEGLGPAIISQLLSKGLIQNVADLYYLKYRDLITLE
RMGDKSARNLLEAIEKSKKRDLDRLLFGLGINLIGSKAAQVIAEHFKSMDNIMKAKYEDFLELPDIGPKMARSIVTFFSE
EQNRRVVERLKEAGVNMEKLSTGKVSNIFEGKIFVLTGALKNYTRDEAARLIVERGGKVTNSVSKKTDYLIVGADPGSKL
KKAQELGVKIINEEQFEAMLKGDIQP
>Mature_666_residues
MDREEARKRIEELREKINYHNYRYYVLDQPEISDYEYDMLMRELIELEEKYPEFKTPDSPSQRVGGEPLDEFEPFTHIVP
MLSLANAFTAEEIKEFDRRVKEAVGEVEYVVEPKIDGLSVELVYENGMFTVGSTRGDGIVGENVTPNLKTIKSIPLRLKD
SVNLVVRGEVFMPKASFAKLNEERAERGESLFANPRNAAAGSVRQLDPKVTAKRDLDIFIFNLQRIEGRDFKTHVEALEF
LKEQGFKVIPLIKKCTTIEEVIKAIEELGEMKDSLPYDIDGAVIKVNELDKREILGQTAKDYRWAIAFKYPAEMKKTKIV
DIVVQVGRTGALTPTAVLEPVVISGSVVSRATLHNEDYIKEKDIRIGDTVLVHKAGGIIPEVVEVVKEERTGDEKAFVMP
DKCPECGALAVRLPGEAVRRCTGLNCPAQIARRIIHFASKDAMDIEGLGPAIISQLLSKGLIQNVADLYYLKYRDLITLE
RMGDKSARNLLEAIEKSKKRDLDRLLFGLGINLIGSKAAQVIAEHFKSMDNIMKAKYEDFLELPDIGPKMARSIVTFFSE
EQNRRVVERLKEAGVNMEKLSTGKVSNIFEGKIFVLTGALKNYTRDEAARLIVERGGKVTNSVSKKTDYLIVGADPGSKL
KKAQELGVKIINEEQFEAMLKGDIQP

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Homo sapiens, GI32528306, Length=69, Percent_Identity=57.9710144927536, Blast_Score=74, Evalue=5e-13,
Organism=Escherichia coli, GI1788750, Length=668, Percent_Identity=45.6586826347305, Blast_Score=578, Evalue=1e-166,
Organism=Escherichia coli, GI87082305, Length=572, Percent_Identity=22.5524475524476, Blast_Score=114, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_THETN (Q8RC42)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_622271.1
- HSSP:   O87703
- ProteinModelPortal:   Q8RC42
- GeneID:   996408
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE0605
- NMPDR:   fig|273068.3.peg.151
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   CLSK900859
- BioCyc:   TTEN273068:TTE0605-MONOMER
- BRENDA:   6.5.1.2
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 74964; Mature: 74964

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 114-114 BINDING 112-112 BINDING 135-135 BINDING 169-169 BINDING 285-285 BINDING 309-309

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDREEARKRIEELREKINYHNYRYYVLDQPEISDYEYDMLMRELIELEEKYPEFKTPDSP
CCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
SQRVGGEPLDEFEPFTHIVPMLSLANAFTAEEIKEFDRRVKEAVGEVEYVVEPKIDGLSV
HHHCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCEEE
ELVYENGMFTVGSTRGDGIVGENVTPNLKTIKSIPLRLKDSVNLVVRGEVFMPKASFAKL
EEEEECCEEEECCCCCCCCCCCCCCCCHHHHHHCCCEECCCCCEEEECCEECCCHHHHHH
NEERAERGESLFANPRNAAAGSVRQLDPKVTAKRDLDIFIFNLQRIEGRDFKTHVEALEF
HHHHHHCCCHHCCCCCCCCCCCHHHCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHH
LKEQGFKVIPLIKKCTTIEEVIKAIEELGEMKDSLPYDIDGAVIKVNELDKREILGQTAK
HHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHCCCCC
DYRWAIAFKYPAEMKKTKIVDIVVQVGRTGALTPTAVLEPVVISGSVVSRATLHNEDYIK
CCEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCEECCCHHHHHHHCCCCCCC
EKDIRIGDTVLVHKAGGIIPEVVEVVKEERTGDEKAFVMPDKCPECGALAVRLPGEAVRR
CCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCCCEEEECCHHHHHH
CTGLNCPAQIARRIIHFASKDAMDIEGLGPAIISQLLSKGLIQNVADLYYLKYRDLITLE
HCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMGDKSARNLLEAIEKSKKRDLDRLLFGLGINLIGSKAAQVIAEHFKSMDNIMKAKYEDF
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LELPDIGPKMARSIVTFFSEEQNRRVVERLKEAGVNMEKLSTGKVSNIFEGKIFVLTGAL
HCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCEEEEEECHH
KNYTRDEAARLIVERGGKVTNSVSKKTDYLIVGADPGSKLKKAQELGVKIINEEQFEAML
HHCCHHHHHHHHHHCCCCHHHHHHCCCCEEEEECCCCHHHHHHHHCCCEEECHHHHHHHH
KGDIQP
HCCCCC
>Mature Secondary Structure
MDREEARKRIEELREKINYHNYRYYVLDQPEISDYEYDMLMRELIELEEKYPEFKTPDSP
CCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
SQRVGGEPLDEFEPFTHIVPMLSLANAFTAEEIKEFDRRVKEAVGEVEYVVEPKIDGLSV
HHHCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCEEE
ELVYENGMFTVGSTRGDGIVGENVTPNLKTIKSIPLRLKDSVNLVVRGEVFMPKASFAKL
EEEEECCEEEECCCCCCCCCCCCCCCCHHHHHHCCCEECCCCCEEEECCEECCCHHHHHH
NEERAERGESLFANPRNAAAGSVRQLDPKVTAKRDLDIFIFNLQRIEGRDFKTHVEALEF
HHHHHHCCCHHCCCCCCCCCCCHHHCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHH
LKEQGFKVIPLIKKCTTIEEVIKAIEELGEMKDSLPYDIDGAVIKVNELDKREILGQTAK
HHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHCCCCC
DYRWAIAFKYPAEMKKTKIVDIVVQVGRTGALTPTAVLEPVVISGSVVSRATLHNEDYIK
CCEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCEECCCHHHHHHHCCCCCCC
EKDIRIGDTVLVHKAGGIIPEVVEVVKEERTGDEKAFVMPDKCPECGALAVRLPGEAVRR
CCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCCCEEEECCHHHHHH
CTGLNCPAQIARRIIHFASKDAMDIEGLGPAIISQLLSKGLIQNVADLYYLKYRDLITLE
HCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMGDKSARNLLEAIEKSKKRDLDRLLFGLGINLIGSKAAQVIAEHFKSMDNIMKAKYEDF
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LELPDIGPKMARSIVTFFSEEQNRRVVERLKEAGVNMEKLSTGKVSNIFEGKIFVLTGAL
HCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCEEEEEECHH
KNYTRDEAARLIVERGGKVTNSVSKKTDYLIVGADPGSKLKKAQELGVKIINEEQFEAML
HHCCHHHHHHHHHHCCCCHHHHHHCCCCEEEEECCCCHHHHHHHHCCCEEECHHHHHHHH
KGDIQP
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336