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The map label for this gene is purN [H]

Identifier: 20807086

GI number: 20807086

Start: 592516

End: 593139

Strand: Direct

Name: purN [H]

Synonym: TTE0591

Alternate gene names: 20807086

Gene position: 592516-593139 (Clockwise)

Preceding gene: 20807085

Following gene: 20807087

Centisome position: 22.03

GC content: 39.1

Gene sequence:

>624_bases
ATGAGGCTTGTGGTAATGGCATCGGGTAATGGCACAGATCTGCAGTCAATAATCGATGCCATTGAAGCTGGTTATATAAA
AGCTCAGATAATCGGAGTGGTGAGCGATAAAAAAGAAGCCTATGCGCTTGAAAGAGCTAAAAAACACGGCATTCCTGCTT
ACTGCCTTCGAAAAAAGGAATTAAAAGAAAATTTCTTTAAAGAGCTTTTGTCACTTCTTGAAAGCCTAAATCCCGACGGG
ATAATTCTTGCAGGTTTTTTAACAATTTTAAGCGAAGAGATTGTGGAAAGGTTCCCAAATAAAATAATAAATATTCATCC
CTCTCTTATCCCTGCCTTTTGTGGAAAAGGATTTTATGGAATGAGAGTTCATCAAGCGGTTTATGATTATGGCGTTAAAT
ATACTGGATGTACAGTGCACTTTGTGGACAAGGGCACAGATACAGGGCCTATCATTCTCCAGGAAGTGGTAAAGATAGAG
GAGCATGATACCCCCGAAAGTATTGCCAAAAAAGTTTTGGAAGTAGAGCACAAAGTCCTGCCTTATGCGGTTAAACTTTT
TGTTGAAGGGAAATTAAAAGTGGAAGGTAGAAGAGTGAAAGTACTTGACAAGGAGGATTTGTGA

Upstream 100 bases:

>100_bases
ATAAAGAAGAAGTGGAGAAAGCTCTAAAAAGGCTTAAAGAAGTGGGAGAGACAGCTTTTGTGATTGGAGAGATTGTGGAG
GGAGAAGGCGGTGTGATTTT

Downstream 100 bases:

>100_bases
TGTCAAGGAGAGCGTTGATAAGTGTTTCAAAAAAAGATGGAATAGTGGAATTTGCGAAAAAGCTTGAAGACCTGGGTTAT
GAGATAATATCTACTGGCGG

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MRLVVMASGNGTDLQSIIDAIEAGYIKAQIIGVVSDKKEAYALERAKKHGIPAYCLRKKELKENFFKELLSLLESLNPDG
IILAGFLTILSEEIVERFPNKIINIHPSLIPAFCGKGFYGMRVHQAVYDYGVKYTGCTVHFVDKGTDTGPIILQEVVKIE
EHDTPESIAKKVLEVEHKVLPYAVKLFVEGKLKVEGRRVKVLDKEDL

Sequences:

>Translated_207_residues
MRLVVMASGNGTDLQSIIDAIEAGYIKAQIIGVVSDKKEAYALERAKKHGIPAYCLRKKELKENFFKELLSLLESLNPDG
IILAGFLTILSEEIVERFPNKIINIHPSLIPAFCGKGFYGMRVHQAVYDYGVKYTGCTVHFVDKGTDTGPIILQEVVKIE
EHDTPESIAKKVLEVEHKVLPYAVKLFVEGKLKVEGRRVKVLDKEDL
>Mature_207_residues
MRLVVMASGNGTDLQSIIDAIEAGYIKAQIIGVVSDKKEAYALERAKKHGIPAYCLRKKELKENFFKELLSLLESLNPDG
IILAGFLTILSEEIVERFPNKIINIHPSLIPAFCGKGFYGMRVHQAVYDYGVKYTGCTVHFVDKGTDTGPIILQEVVKIE
EHDTPESIAKKVLEVEHKVLPYAVKLFVEGKLKVEGRRVKVLDKEDL

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=195, Percent_Identity=37.9487179487179, Blast_Score=135, Evalue=3e-32,
Organism=Homo sapiens, GI209869995, Length=195, Percent_Identity=37.9487179487179, Blast_Score=135, Evalue=3e-32,
Organism=Homo sapiens, GI209869993, Length=195, Percent_Identity=37.9487179487179, Blast_Score=135, Evalue=3e-32,
Organism=Escherichia coli, GI1788846, Length=196, Percent_Identity=36.2244897959184, Blast_Score=139, Evalue=1e-34,
Organism=Escherichia coli, GI1787483, Length=202, Percent_Identity=31.1881188118812, Blast_Score=102, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17567511, Length=184, Percent_Identity=35.3260869565217, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6320616, Length=196, Percent_Identity=32.1428571428571, Blast_Score=82, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24582400, Length=193, Percent_Identity=37.3056994818653, Blast_Score=132, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23154; Mature: 23154

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLVVMASGNGTDLQSIIDAIEAGYIKAQIIGVVSDKKEAYALERAKKHGIPAYCLRKKE
CEEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHH
LKENFFKELLSLLESLNPDGIILAGFLTILSEEIVERFPNKIINIHPSLIPAFCGKGFYG
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHEECCHHHHHHHHCCCCCH
MRVHQAVYDYGVKYTGCTVHFVDKGTDTGPIILQEVVKIEEHDTPESIAKKVLEVEHKVL
HHHHHHHHHCCCEECCEEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
PYAVKLFVEGKLKVEGRRVKVLDKEDL
HHHHHEEECCEEEECCCEEEEECCCCC
>Mature Secondary Structure
MRLVVMASGNGTDLQSIIDAIEAGYIKAQIIGVVSDKKEAYALERAKKHGIPAYCLRKKE
CEEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHH
LKENFFKELLSLLESLNPDGIILAGFLTILSEEIVERFPNKIINIHPSLIPAFCGKGFYG
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHEECCHHHHHHHHCCCCCH
MRVHQAVYDYGVKYTGCTVHFVDKGTDTGPIILQEVVKIEEHDTPESIAKKVLEVEHKVL
HHHHHHHHHCCCEECCEEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
PYAVKLFVEGKLKVEGRRVKVLDKEDL
HHHHHEEECCEEEECCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]