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The map label for this gene is ArgF

Identifier: 20807033

GI number: 20807033

Start: 539545

End: 540486

Strand: Direct

Name: ArgF

Synonym: TTE0532

Alternate gene names: 20807033

Gene position: 539545-540486 (Clockwise)

Preceding gene: 20807032

Following gene: 20807034

Centisome position: 20.06

GC content: 42.25

Gene sequence:

>942_bases
ATGGCATTCAATTTAAAGGGTAGAAGTCTTCTTACTTTAAAAGAGTACACTCCACAGGAGATAAGGTATCTTTTGGACAT
TGCGAAACAGGTAAAGGCGGAGAGAAAGGCTGGAATAGTGCATCAGAGGTTTGTAGGTAAGACTATTGCTTTGATATTTG
AAAAAAGGTCAACGAGAACTCGTTGTGCTTTTGAGACTGCCTTTGGAGAAGAAGGAGGGCATCCGGTATTTCTGTCTACA
GATGACATACAGTTAGGGGCAAAAGAGTCAATTGAGGACACCGCAAGAGTTTTAGGCAGAATGTTTGATGCGATAGAGTT
CAGAGGTTTCAAGCAGGAGACAGTGGAGATCCTAGCTAAGTATGCCGGGGTCCCTGTATACAATGGTTTGACTGATGAGT
ATCATCCAACTCAGGTGTTAGCCGATTTGATGACAATAGAAGAGGAATTTGGCAGTTTAAAGGGAAGAAAACTTGTATTT
GTAGGAGACGGAAGAAACAATATGGCTAATACTCTGGCTATAGGTTGTGCAAAAATGGGCATGCACTATGTGATAAATTC
ACCAAAGGAGCTCTGGCCTTCGGAGTCCTTTATAAAGGAAATTAAAGATATGGCCGCAGAAAATGGAGGAACTTTCGAAC
TCACAAGTGTACCTGGAGAAGGTCTTGAAGGAGCAGATGCCATCTATACTGACGTGTGGGCTTCAATGGGAGAAGAAGCA
AAGGCAGAGGAAAGGGAAAGGCTTTTAAGGCCATATCAGGTGAATGAAGAAATGATGAAAAAGACAGGAAGAGATGACAC
GATATTCTTGCATTGCTTACCGGCGGTAAAAGGGCAGGAAGTCACTTTTGAGGTTATAGAAGGCAAGCAATCAAGAGTTT
GGGACCAAGCAGAGAATAGAAAACACACCATTAAGGCTGTTCTGATTGCTACTTTGCTTTAA

Upstream 100 bases:

>100_bases
GTCTATGCCTCTTTTAAGAGAGGAAATCTAAAAAAGCTTGACTAAAGGATTGAGGATAAGATAAAATATTTTAAGGCAAA
TTTACAAAAGGAGGATGGCT

Downstream 100 bases:

>100_bases
TTTAAACTTAGGGGAGAAAGGTATGAGAGAGAGAGTTGTTGTGGCTTTAGGAGGCAACGCCCTGCAGGATGTGAACACCC
CTCCTACTGCAGAGGCGCAG

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MAFNLKGRSLLTLKEYTPQEIRYLLDIAKQVKAERKAGIVHQRFVGKTIALIFEKRSTRTRCAFETAFGEEGGHPVFLST
DDIQLGAKESIEDTARVLGRMFDAIEFRGFKQETVEILAKYAGVPVYNGLTDEYHPTQVLADLMTIEEEFGSLKGRKLVF
VGDGRNNMANTLAIGCAKMGMHYVINSPKELWPSESFIKEIKDMAAENGGTFELTSVPGEGLEGADAIYTDVWASMGEEA
KAEERERLLRPYQVNEEMMKKTGRDDTIFLHCLPAVKGQEVTFEVIEGKQSRVWDQAENRKHTIKAVLIATLL

Sequences:

>Translated_313_residues
MAFNLKGRSLLTLKEYTPQEIRYLLDIAKQVKAERKAGIVHQRFVGKTIALIFEKRSTRTRCAFETAFGEEGGHPVFLST
DDIQLGAKESIEDTARVLGRMFDAIEFRGFKQETVEILAKYAGVPVYNGLTDEYHPTQVLADLMTIEEEFGSLKGRKLVF
VGDGRNNMANTLAIGCAKMGMHYVINSPKELWPSESFIKEIKDMAAENGGTFELTSVPGEGLEGADAIYTDVWASMGEEA
KAEERERLLRPYQVNEEMMKKTGRDDTIFLHCLPAVKGQEVTFEVIEGKQSRVWDQAENRKHTIKAVLIATLL
>Mature_312_residues
AFNLKGRSLLTLKEYTPQEIRYLLDIAKQVKAERKAGIVHQRFVGKTIALIFEKRSTRTRCAFETAFGEEGGHPVFLSTD
DIQLGAKESIEDTARVLGRMFDAIEFRGFKQETVEILAKYAGVPVYNGLTDEYHPTQVLADLMTIEEEFGSLKGRKLVFV
GDGRNNMANTLAIGCAKMGMHYVINSPKELWPSESFIKEIKDMAAENGGTFELTSVPGEGLEGADAIYTDVWASMGEEAK
AEERERLLRPYQVNEEMMKKTGRDDTIFLHCLPAVKGQEVTFEVIEGKQSRVWDQAENRKHTIKAVLIATLL

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI38788445, Length=309, Percent_Identity=45.3074433656958, Blast_Score=257, Evalue=8e-69,
Organism=Homo sapiens, GI18105007, Length=322, Percent_Identity=26.7080745341615, Blast_Score=90, Evalue=2e-18,
Organism=Escherichia coli, GI1790703, Length=326, Percent_Identity=48.4662576687117, Blast_Score=306, Evalue=8e-85,
Organism=Escherichia coli, GI1786469, Length=330, Percent_Identity=49.0909090909091, Blast_Score=304, Evalue=6e-84,
Organism=Escherichia coli, GI48994908, Length=362, Percent_Identity=25.9668508287293, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI2367364, Length=237, Percent_Identity=30.379746835443, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI193204318, Length=320, Percent_Identity=25, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6322373, Length=308, Percent_Identity=35.7142857142857, Blast_Score=189, Evalue=5e-49,
Organism=Saccharomyces cerevisiae, GI6322331, Length=280, Percent_Identity=29.2857142857143, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24642586, Length=324, Percent_Identity=26.5432098765432, Blast_Score=82, Evalue=3e-16,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): OTC_THETN (Q8RCA4)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_622204.1
- ProteinModelPortal:   Q8RCA4
- SMR:   Q8RCA4
- GeneID:   996044
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE0532
- NMPDR:   fig|273068.3.peg.979
- HOGENOM:   HBG579429
- OMA:   NHETVES
- ProtClustDB:   PRK02102
- BioCyc:   TTEN273068:TTE0532-MONOMER
- BRENDA:   2.1.3.3
- HAMAP:   MF_01109
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292
- PRINTS:   PR00100
- PRINTS:   PR00102
- TIGRFAMs:   TIGR00658

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.3

Molecular weight: Translated: 35149; Mature: 35018

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: BINDING 108-108 BINDING 135-135

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFNLKGRSLLTLKEYTPQEIRYLLDIAKQVKAERKAGIVHQRFVGKTIALIFEKRSTRT
CEECCCCCEEEEEHHCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCH
RCAFETAFGEEGGHPVFLSTDDIQLGAKESIEDTARVLGRMFDAIEFRGFKQETVEILAK
HHHHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
YAGVPVYNGLTDEYHPTQVLADLMTIEEEFGSLKGRKLVFVGDGRNNMANTLAIGCAKMG
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHC
MHYVINSPKELWPSESFIKEIKDMAAENGGTFELTSVPGEGLEGADAIYTDVWASMGEEA
HHHEECCCHHHCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCHH
KAEERERLLRPYQVNEEMMKKTGRDDTIFLHCLPAVKGQEVTFEVIEGKQSRVWDQAENR
HHHHHHHHCCCCCCCHHHHHHCCCCCEEEEEEECCCCCCCEEEHHHCCCHHHHHHHHHCH
KHTIKAVLIATLL
HHHHHHHHHHHCC
>Mature Secondary Structure 
AFNLKGRSLLTLKEYTPQEIRYLLDIAKQVKAERKAGIVHQRFVGKTIALIFEKRSTRT
EECCCCCEEEEEHHCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCH
RCAFETAFGEEGGHPVFLSTDDIQLGAKESIEDTARVLGRMFDAIEFRGFKQETVEILAK
HHHHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
YAGVPVYNGLTDEYHPTQVLADLMTIEEEFGSLKGRKLVFVGDGRNNMANTLAIGCAKMG
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHC
MHYVINSPKELWPSESFIKEIKDMAAENGGTFELTSVPGEGLEGADAIYTDVWASMGEEA
HHHEECCCHHHCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCHH
KAEERERLLRPYQVNEEMMKKTGRDDTIFLHCLPAVKGQEVTFEVIEGKQSRVWDQAENR
HHHHHHHHCCCCCCCHHHHHHCCCCCEEEEEEECCCCCCCEEEHHHCCCHHHHHHHHHCH
KHTIKAVLIATLL
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336