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The map label for this gene is LplA [H]

Identifier: 20806818

GI number: 20806818

Start: 313277

End: 314257

Strand: Direct

Name: LplA [H]

Synonym: TTE0297

Alternate gene names: 20806818

Gene position: 313277-314257 (Clockwise)

Preceding gene: 20806813

Following gene: 20806819

Centisome position: 11.65

GC content: 36.19

Gene sequence:

>981_bases
ATGCTGTATATTTATAACAAAAACACAAATCCCTATTTCAACTTGGCAGCTGAAGAATACGTTTTAAAGGAATTCAAAGA
CGAATGCTTTATGCTCTGGCGCAATGAACCCAGCATAATAGTTGGCAAAAATCAAAATACTCTGGCAGAGATAAATTTGG
ACTATGTAAGAGAACACAAAATTCCCGTTGTCAGAAGGCTATCAGGGGGAGGGGCGGTTTTTCACGACTTAGGCAACTTA
AACTTTACGTTTATTGTAAATGCAGATGAGGGTGGATTTACAGATTTTAAAAGGTTTGCACAGCCTATTATAGACGTTCT
TAGAAAATTAGGAGTGGAAGCAGAGTTTTCCGGCAGAAATGATATAACCATTGATGGGAAAAAGATATCTGGAAATGCCC
AGTATTATTACAAGAATAGGATTCTGCACCACGGAACTTTGCTCTTTTCATCAAATATTACAGACCTGTCAGCTGCTTTA
AAGGTGAGGCCTGTTAAGTTTGAAGATAAGGGAGTTAAGTCTGTATCCAAAAGAGTGGCGAATATAAGCGAATATTTAAA
AGAGCCTATCACTATAGAGGAATTTATAAATTTAATAATGGAACACATAAAGGAGCAAACTGGCGGAGGAGAGATGTACG
AATTTACAGAAGAGGATTTAAAAAAGATAAACAAATTAGTAGAAGAGAAATACGGCACTTGGGAGTGGAATTTCGGGTCT
TCTCCCGACTATAAAATGAAGCATGAGAAAAAATTCCCCGGCGGTACTGTAGAAGTCAATCTAAATGTAGAAAAGGGTAT
AATAAAAGATGTAAAAATATACGGAGATTTCTTTGGGAAGTACGACATTTCTGAAGTGGAGGATTTATTAAAAGGGGTAA
AGCACTCTGAGGAAGAGGTGAGGAAGGCTCTTTCAAAGATAAATCTTCAAGATTATATGGCAAATATAACTATTGATAAT
TTGATTGAGGTGATGTTTTAA

Upstream 100 bases:

>100_bases
TCAATTTTTTTTAAAATTATGCTATACTTTTACCATCATTATATATTATCCATAGACTTTAAGTCAACAATCGTTAAAAT
TGAGACGAGGGGTGGAGTGT

Downstream 100 bases:

>100_bases
AAATTTTTTCTTTTTTTACCTCATAGTGTAAAATTATCGTAGGATTTTTTGAGTGAAAAAAACAAGAGACAACCCCTGTG
ATAAAATAGATTGTGGAAAA

Product: lipoate-protein ligase A

Products: NA

Alternate protein names: Lipoate--protein ligase [H]

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MLYIYNKNTNPYFNLAAEEYVLKEFKDECFMLWRNEPSIIVGKNQNTLAEINLDYVREHKIPVVRRLSGGGAVFHDLGNL
NFTFIVNADEGGFTDFKRFAQPIIDVLRKLGVEAEFSGRNDITIDGKKISGNAQYYYKNRILHHGTLLFSSNITDLSAAL
KVRPVKFEDKGVKSVSKRVANISEYLKEPITIEEFINLIMEHIKEQTGGGEMYEFTEEDLKKINKLVEEKYGTWEWNFGS
SPDYKMKHEKKFPGGTVEVNLNVEKGIIKDVKIYGDFFGKYDISEVEDLLKGVKHSEEEVRKALSKINLQDYMANITIDN
LIEVMF

Sequences:

>Translated_326_residues
MLYIYNKNTNPYFNLAAEEYVLKEFKDECFMLWRNEPSIIVGKNQNTLAEINLDYVREHKIPVVRRLSGGGAVFHDLGNL
NFTFIVNADEGGFTDFKRFAQPIIDVLRKLGVEAEFSGRNDITIDGKKISGNAQYYYKNRILHHGTLLFSSNITDLSAAL
KVRPVKFEDKGVKSVSKRVANISEYLKEPITIEEFINLIMEHIKEQTGGGEMYEFTEEDLKKINKLVEEKYGTWEWNFGS
SPDYKMKHEKKFPGGTVEVNLNVEKGIIKDVKIYGDFFGKYDISEVEDLLKGVKHSEEEVRKALSKINLQDYMANITIDN
LIEVMF
>Mature_326_residues
MLYIYNKNTNPYFNLAAEEYVLKEFKDECFMLWRNEPSIIVGKNQNTLAEINLDYVREHKIPVVRRLSGGGAVFHDLGNL
NFTFIVNADEGGFTDFKRFAQPIIDVLRKLGVEAEFSGRNDITIDGKKISGNAQYYYKNRILHHGTLLFSSNITDLSAAL
KVRPVKFEDKGVKSVSKRVANISEYLKEPITIEEFINLIMEHIKEQTGGGEMYEFTEEDLKKINKLVEEKYGTWEWNFGS
SPDYKMKHEKKFPGGTVEVNLNVEKGIIKDVKIYGDFFGKYDISEVEDLLKGVKHSEEEVRKALSKINLQDYMANITIDN
LIEVMF

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes [H]

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

Organism=Homo sapiens, GI7706252, Length=245, Percent_Identity=31.8367346938775, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI21729884, Length=245, Percent_Identity=31.8367346938775, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI21729882, Length=245, Percent_Identity=31.8367346938775, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI21729880, Length=245, Percent_Identity=31.8367346938775, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI21729878, Length=245, Percent_Identity=31.8367346938775, Blast_Score=112, Evalue=6e-25,
Organism=Escherichia coli, GI1790846, Length=298, Percent_Identity=34.2281879194631, Blast_Score=169, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI71981909, Length=256, Percent_Identity=29.6875, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6322415, Length=284, Percent_Identity=30.9859154929577, Blast_Score=117, Evalue=2e-27,
Organism=Drosophila melanogaster, GI28573464, Length=331, Percent_Identity=26.2839879154079, Blast_Score=122, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 37543; Mature: 37543

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYIYNKNTNPYFNLAAEEYVLKEFKDECFMLWRNEPSIIVGKNQNTLAEINLDYVREHK
CEEEEECCCCCEEEEHHHHHHHHHHHHHEEEEECCCCCEEEECCCCEEEEEEHHHHHHCC
IPVVRRLSGGGAVFHDLGNLNFTFIVNADEGGFTDFKRFAQPIIDVLRKLGVEAEFSGRN
CCEEEEECCCCEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCC
DITIDGKKISGNAQYYYKNRILHHGTLLFSSNITDLSAALKVRPVKFEDKGVKSVSKRVA
CEEEECEEECCCEEEEEECEEEECCEEEEECCCHHHHHHHEEECEEECCCHHHHHHHHHH
NISEYLKEPITIEEFINLIMEHIKEQTGGGEMYEFTEEDLKKINKLVEEKYGTWEWNFGS
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCC
SPDYKMKHEKKFPGGTVEVNLNVEKGIIKDVKIYGDFFGKYDISEVEDLLKGVKHSEEEV
CCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHH
RKALSKINLQDYMANITIDNLIEVMF
HHHHHHCCHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure
MLYIYNKNTNPYFNLAAEEYVLKEFKDECFMLWRNEPSIIVGKNQNTLAEINLDYVREHK
CEEEEECCCCCEEEEHHHHHHHHHHHHHEEEEECCCCCEEEECCCCEEEEEEHHHHHHCC
IPVVRRLSGGGAVFHDLGNLNFTFIVNADEGGFTDFKRFAQPIIDVLRKLGVEAEFSGRN
CCEEEEECCCCEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCC
DITIDGKKISGNAQYYYKNRILHHGTLLFSSNITDLSAALKVRPVKFEDKGVKSVSKRVA
CEEEECEEECCCEEEEEECEEEECCEEEEECCCHHHHHHHEEECEEECCCHHHHHHHHHH
NISEYLKEPITIEEFINLIMEHIKEQTGGGEMYEFTEEDLKKINKLVEEKYGTWEWNFGS
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCC
SPDYKMKHEKKFPGGTVEVNLNVEKGIIKDVKIYGDFFGKYDISEVEDLLKGVKHSEEEV
CCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHH
RKALSKINLQDYMANITIDNLIEVMF
HHHHHHCCHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]