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The map label for this gene is SbcD [H]

Identifier: 20806786

GI number: 20806786

Start: 274773

End: 275975

Strand: Direct

Name: SbcD [H]

Synonym: TTE0265

Alternate gene names: 20806786

Gene position: 274773-275975 (Clockwise)

Preceding gene: 20806785

Following gene: 20806787

Centisome position: 10.22

GC content: 41.23

Gene sequence:

>1203_bases
ATGCGGATTTTACACACTTCAGATTGGCATTTAGGGAAAACTCTCGAAAATTTTTCAAGGCTTGCCGAACAAGAAAAATT
TTTAGATGATTTTGTAAAAATAGCTGAAGAGAATGACGTGGATTTGGTGATAATAGCTGGAGATGTCTATGACTCTTCAA
ACCCTCCTGCAAAGGCAGAAATGCTGTTTTACAATACTTTAAAAAGACTCGCCAATGGAGAAAGAGTTATTTTGGCAATT
GCAGGCAATCACGATAACCCGGAAAGGCTTTCTGCCGCAAGCCCTCTTGCCTATGAACAGGGAGTAATACTTTTGGGGTC
TACTAAAACTATTGTGCCAACAGGAGACTTTGGCAGGTTTAAGATTCACAATTCAGGGGAAGGCTTTTTTGAGGTAGAGA
TAAAAGGGGAAAGGGCTGTTGTGATAGCCCTTCCCTACCCCAGCGAGAAAAGGCTTAATGAGATTTTTTCTGAAAGCCTG
GAGGAGGAAGAGAGGCAGAAGAGCTATTCTGAAAGAGTCGGCAGGCTTTTTGAAGAGCTTTCAAAAAATTACAGGGAAGA
TACTATAAACATAGCTGTTTCGCACATTTTTGTGGCAGGGGGAGAGGAAGCAGGCTCAGAAAGGCCTATTCAGCTTGGAG
GAAGTTTTACTGTGGAGTTAAGGCATCTTCCCGAAAAGGCTCAGTACATTGCTTTGGGGCACCTTCACAAGCCTCAAAGG
GTTTCTGATGTACTGCCTGTGTACTATTCAGGTTCTCCTCTTCAGTACAGCAAGAGCGAGATGAATCAATCAAAATGCGC
ATATCTGGTGGATTTAAAAGCGGGAGAGAAAGCTTACGTAAAAGAAATTTACTTTAAAAATTATAAGCCCATTGAGGTTT
TCAGGTGCGATGGGATAGATGAAGCTCTAAAAATTTGTGAAGAGTATAAGGACAAGGATGTGTGGGCTTATTTTGAAATA
AAAACAGAATCTCCTCTTCCTTCCTCTCAGATAAGGGAAATGAAAAGGCTTATGCCTGATATAGTAGAGATAAGGCCAAT
CCTTCCGGAAGAAGAGGATGTCTTTGAAGAAATCGAGGTAGAGGATAAAGACATAAGAGAGCTGTTTCAGGAGTTTTACC
TAAAAGAGAAGAAAGTTCCTCCTTCAGAAGAGATGGTGGAACTTTTTATGAGCATAATTGGGAAAGAGGATGAGGGAGCA
TGA

Upstream 100 bases:

>100_bases
TATGGAAAGGCTTTAGAAGAAATCACGGGAAAGAAGGTAAAAGAGAAGTACATCTACCTCTTTTTTAATGATACTATCAT
AAAGTACTAAGGGTGATAAA

Downstream 100 bases:

>100_bases
AGCCTATTAGACTTAAAATTTCTGGCGTTAACAGCTTTACAGAAGAGCAAGTCATAAATTTTGAACTTCTCACTGAAAAA
GGGCTTTTTGGAATATTTGG

Product: DNA repair exonuclease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MRILHTSDWHLGKTLENFSRLAEQEKFLDDFVKIAEENDVDLVIIAGDVYDSSNPPAKAEMLFYNTLKRLANGERVILAI
AGNHDNPERLSAASPLAYEQGVILLGSTKTIVPTGDFGRFKIHNSGEGFFEVEIKGERAVVIALPYPSEKRLNEIFSESL
EEEERQKSYSERVGRLFEELSKNYREDTINIAVSHIFVAGGEEAGSERPIQLGGSFTVELRHLPEKAQYIALGHLHKPQR
VSDVLPVYYSGSPLQYSKSEMNQSKCAYLVDLKAGEKAYVKEIYFKNYKPIEVFRCDGIDEALKICEEYKDKDVWAYFEI
KTESPLPSSQIREMKRLMPDIVEIRPILPEEEDVFEEIEVEDKDIRELFQEFYLKEKKVPPSEEMVELFMSIIGKEDEGA

Sequences:

>Translated_400_residues
MRILHTSDWHLGKTLENFSRLAEQEKFLDDFVKIAEENDVDLVIIAGDVYDSSNPPAKAEMLFYNTLKRLANGERVILAI
AGNHDNPERLSAASPLAYEQGVILLGSTKTIVPTGDFGRFKIHNSGEGFFEVEIKGERAVVIALPYPSEKRLNEIFSESL
EEEERQKSYSERVGRLFEELSKNYREDTINIAVSHIFVAGGEEAGSERPIQLGGSFTVELRHLPEKAQYIALGHLHKPQR
VSDVLPVYYSGSPLQYSKSEMNQSKCAYLVDLKAGEKAYVKEIYFKNYKPIEVFRCDGIDEALKICEEYKDKDVWAYFEI
KTESPLPSSQIREMKRLMPDIVEIRPILPEEEDVFEEIEVEDKDIRELFQEFYLKEKKVPPSEEMVELFMSIIGKEDEGA
>Mature_400_residues
MRILHTSDWHLGKTLENFSRLAEQEKFLDDFVKIAEENDVDLVIIAGDVYDSSNPPAKAEMLFYNTLKRLANGERVILAI
AGNHDNPERLSAASPLAYEQGVILLGSTKTIVPTGDFGRFKIHNSGEGFFEVEIKGERAVVIALPYPSEKRLNEIFSESL
EEEERQKSYSERVGRLFEELSKNYREDTINIAVSHIFVAGGEEAGSERPIQLGGSFTVELRHLPEKAQYIALGHLHKPQR
VSDVLPVYYSGSPLQYSKSEMNQSKCAYLVDLKAGEKAYVKEIYFKNYKPIEVFRCDGIDEALKICEEYKDKDVWAYFEI
KTESPLPSSQIREMKRLMPDIVEIRPILPEEEDVFEEIEVEDKDIRELFQEFYLKEKKVPPSEEMVELFMSIIGKEDEGA

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=367, Percent_Identity=26.9754768392371, Blast_Score=121, Evalue=8e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 45779; Mature: 45779

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILHTSDWHLGKTLENFSRLAEQEKFLDDFVKIAEENDVDLVIIAGDVYDSSNPPAKAE
CEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHH
MLFYNTLKRLANGERVILAIAGNHDNPERLSAASPLAYEQGVILLGSTKTIVPTGDFGRF
HHHHHHHHHHCCCCEEEEEEECCCCCHHHHHCCCCCCHHCCEEEEECCEEEEECCCCCEE
KIHNSGEGFFEVEIKGERAVVIALPYPSEKRLNEIFSESLEEEERQKSYSERVGRLFEEL
EEECCCCCEEEEEEECCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SKNYREDTINIAVSHIFVAGGEEAGSERPIQLGGSFTVELRHLPEKAQYIALGHLHKPQR
HHHHHHHHHEEEEEEEEEECCCCCCCCCCEEECCEEEEEEECCCCHHCEEEECCCCCCHH
VSDVLPVYYSGSPLQYSKSEMNQSKCAYLVDLKAGEKAYVKEIYFKNYKPIEVFRCDGID
HHHCCCEEECCCCCCCCHHHCCCCCCEEEEEECCCCHHHHHHHHHCCCCCEEEEEECCHH
EALKICEEYKDKDVWAYFEIKTESPLPSSQIREMKRLMPDIVEIRPILPEEEDVFEEIEV
HHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCC
EDKDIRELFQEFYLKEKKVPPSEEMVELFMSIIGKEDEGA
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRILHTSDWHLGKTLENFSRLAEQEKFLDDFVKIAEENDVDLVIIAGDVYDSSNPPAKAE
CEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHH
MLFYNTLKRLANGERVILAIAGNHDNPERLSAASPLAYEQGVILLGSTKTIVPTGDFGRF
HHHHHHHHHHCCCCEEEEEEECCCCCHHHHHCCCCCCHHCCEEEEECCEEEEECCCCCEE
KIHNSGEGFFEVEIKGERAVVIALPYPSEKRLNEIFSESLEEEERQKSYSERVGRLFEEL
EEECCCCCEEEEEEECCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SKNYREDTINIAVSHIFVAGGEEAGSERPIQLGGSFTVELRHLPEKAQYIALGHLHKPQR
HHHHHHHHHEEEEEEEEEECCCCCCCCCCEEECCEEEEEEECCCCHHCEEEECCCCCCHH
VSDVLPVYYSGSPLQYSKSEMNQSKCAYLVDLKAGEKAYVKEIYFKNYKPIEVFRCDGID
HHHCCCEEECCCCCCCCHHHCCCCCCEEEEEECCCCHHHHHHHHHCCCCCEEEEEECCHH
EALKICEEYKDKDVWAYFEIKTESPLPSSQIREMKRLMPDIVEIRPILPEEEDVFEEIEV
HHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCC
EDKDIRELFQEFYLKEKKVPPSEEMVELFMSIIGKEDEGA
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377; 10568751; 1646786; 8493111 [H]