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The map label for this gene is BglX [H]

Identifier: 20806741

GI number: 20806741

Start: 221759

End: 223339

Strand: Direct

Name: BglX [H]

Synonym: TTE0215

Alternate gene names: 20806741

Gene position: 221759-223339 (Clockwise)

Preceding gene: 20806740

Following gene: 20806742

Centisome position: 8.25

GC content: 36.12

Gene sequence:

>1581_bases
ATGAAGAAAATAGAAGAAATGACATTGGAAGAAAAAATAGGGCAAATGCTGATGATAGGTTTTCCGTCAGCTTTTTACGA
CGACCATATAAGAGAATTAGTAACAAGTTATAAAATAGGGAATGTAATACTCTTTTCAAGAAATGTGCAGGATGCAAGGC
AAGTAATGGATTTATGCACTGACATACAGAAAGAAGCAATAGAAAATACCGGAATACCAGCTTTTATCTCTATTGACCAG
GAAGGTGGCATGGTCACAAGGATTTACAGAGGAGCTACTTATTTTCCCGGCAATATGGCTATTGGTGCTACAAAAGACCC
AGAAAATGCTTATAAAGTGGGAGAAATAGCTGGAAGAGAATTAAGAACTATAGGCATAAACATAAATTTCGCTCCGGTTA
TGGATGTGAACAATAACCCTTTAAATCCAGTGATAGGAGTCAGGTCTTACGGTGAAAACCCAGAGGATGTGGCTAATTTT
GGAATAAACTACATAAAAGGGCTTCAAAAAGAAGGTGTGATAGCTACAGCTAAACATTTCCCAGGACATGGAGACACAAC
TGTGGATTCTCACCTTGATTTACCGAAAGTCAATCATGGCAAGGATAGGCTTTATGAAGTGGAATTATATCCGTTTAAGA
AAGCTGTAGAAAATGGCGTAGATGCGATAATGACAGCTCATATACTTTTTCCGGCTTTAGAAGATAGTAAAGTTCCTGCA
ACTCTTTCTTATAATATCCTTACCCGCATCTTAAGGGAAGAATTCAAGTTTGATGGGCTTGTTATAACTGACTGTATGGA
AATGAATGCGATAGCCAAATATTTTGGCACTCAAAAGGCAGCGTCAATGGCTATAAAAGCTGGAGCAGATATAGTACTTG
TTTCTCATACTAAAGAGCTACAGATAAAAGCTTTTGAAAATATAAAAGAAGCTGTAGAAAGAGGAGATATCTCTGTAGAA
AGAATAGATGAATCAGTAAGAAGGATACTCAAGATGAAAGAAAAATATGGACTTTTTGATAGGCCATATCCTGAGAAAGA
CAAATTTGACACCACAGTTGGAATTGAGAAACATAGAAAAATTGCAAAAGCCATAAGTTTAAAGAGCATAACAGTTGTTA
AAGATGAGAAAAAATTAATCCCTTTAAAGACGAAAAATGTACTGGCAATTTCTCCTGAAACAGTTCCGGTTTCTTTTGTA
GACGATGCTTTGATGGATAGGCTTTCTTTTGCAAGACAATTTACTTCTACATTTGGAGGAGTAGAAGAAACTATTTCTGT
AAATCCAAACAATAGAGAAATCTCAGTAATTCTAGAAAAGGCAAAGGATAAAGAAGTAGTTGTCGTAGGTACTTACAATG
CTAATTTAAATGAAGGTCAAGTAAAACTAGTAAACGCACTTTTGCAAGTGAACAAAAATGTAATTGTTGTAGCTTTGAGA
AATCCTTATGACCTTCAAAAGTTTGAAAATGTGCCTACTTATGTATGTGCTTACGAGTACACCCCATTGTCAGTAGAAAG
CGTGATAGGAGTCTTGACGGGGAAATATCCTGCTGAAGGAAAACTTCCTGTGACCATATGA

Upstream 100 bases:

>100_bases
ATGACGAGGATCAGTTATTGGACAAATGGAAAAGAGAAAGTGAAGAGTTTAAAAGATTGAAAGAGAGTTATCATTTCTAT
GAATAGAGAGGATTGAAAAA

Downstream 100 bases:

>100_bases
ATTTAGGGAAGTGAAGAAATGGAATATGTAGTTGGAATTGATGGAGGAGGCACAAAAAGCGCAATTTCTATCCTAGATTT
GCAGGGAAATGTCCTTTTTA

Product: Beta-glucosidase-related glycosidase

Products: NA

Alternate protein names: ORF1 [H]

Number of amino acids: Translated: 526; Mature: 526

Protein sequence:

>526_residues
MKKIEEMTLEEKIGQMLMIGFPSAFYDDHIRELVTSYKIGNVILFSRNVQDARQVMDLCTDIQKEAIENTGIPAFISIDQ
EGGMVTRIYRGATYFPGNMAIGATKDPENAYKVGEIAGRELRTIGININFAPVMDVNNNPLNPVIGVRSYGENPEDVANF
GINYIKGLQKEGVIATAKHFPGHGDTTVDSHLDLPKVNHGKDRLYEVELYPFKKAVENGVDAIMTAHILFPALEDSKVPA
TLSYNILTRILREEFKFDGLVITDCMEMNAIAKYFGTQKAASMAIKAGADIVLVSHTKELQIKAFENIKEAVERGDISVE
RIDESVRRILKMKEKYGLFDRPYPEKDKFDTTVGIEKHRKIAKAISLKSITVVKDEKKLIPLKTKNVLAISPETVPVSFV
DDALMDRLSFARQFTSTFGGVEETISVNPNNREISVILEKAKDKEVVVVGTYNANLNEGQVKLVNALLQVNKNVIVVALR
NPYDLQKFENVPTYVCAYEYTPLSVESVIGVLTGKYPAEGKLPVTI

Sequences:

>Translated_526_residues
MKKIEEMTLEEKIGQMLMIGFPSAFYDDHIRELVTSYKIGNVILFSRNVQDARQVMDLCTDIQKEAIENTGIPAFISIDQ
EGGMVTRIYRGATYFPGNMAIGATKDPENAYKVGEIAGRELRTIGININFAPVMDVNNNPLNPVIGVRSYGENPEDVANF
GINYIKGLQKEGVIATAKHFPGHGDTTVDSHLDLPKVNHGKDRLYEVELYPFKKAVENGVDAIMTAHILFPALEDSKVPA
TLSYNILTRILREEFKFDGLVITDCMEMNAIAKYFGTQKAASMAIKAGADIVLVSHTKELQIKAFENIKEAVERGDISVE
RIDESVRRILKMKEKYGLFDRPYPEKDKFDTTVGIEKHRKIAKAISLKSITVVKDEKKLIPLKTKNVLAISPETVPVSFV
DDALMDRLSFARQFTSTFGGVEETISVNPNNREISVILEKAKDKEVVVVGTYNANLNEGQVKLVNALLQVNKNVIVVALR
NPYDLQKFENVPTYVCAYEYTPLSVESVIGVLTGKYPAEGKLPVTI
>Mature_526_residues
MKKIEEMTLEEKIGQMLMIGFPSAFYDDHIRELVTSYKIGNVILFSRNVQDARQVMDLCTDIQKEAIENTGIPAFISIDQ
EGGMVTRIYRGATYFPGNMAIGATKDPENAYKVGEIAGRELRTIGININFAPVMDVNNNPLNPVIGVRSYGENPEDVANF
GINYIKGLQKEGVIATAKHFPGHGDTTVDSHLDLPKVNHGKDRLYEVELYPFKKAVENGVDAIMTAHILFPALEDSKVPA
TLSYNILTRILREEFKFDGLVITDCMEMNAIAKYFGTQKAASMAIKAGADIVLVSHTKELQIKAFENIKEAVERGDISVE
RIDESVRRILKMKEKYGLFDRPYPEKDKFDTTVGIEKHRKIAKAISLKSITVVKDEKKLIPLKTKNVLAISPETVPVSFV
DDALMDRLSFARQFTSTFGGVEETISVNPNNREISVILEKAKDKEVVVVGTYNANLNEGQVKLVNALLQVNKNVIVVALR
NPYDLQKFENVPTYVCAYEYTPLSVESVIGVLTGKYPAEGKLPVTI

Specific function: Hydrolyses the beta-1,4-glycosidic bond between N-acetylglucosamine and anhydro-N-acetylmuramic acid in cell wall degradation products following their importation into the cell during the process for recycling cell wall muropeptides [C]

COG id: COG1472

COG function: function code G; Beta-glucosidase-related glycosidases

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787350, Length=325, Percent_Identity=29.8461538461538, Blast_Score=148, Evalue=8e-37,
Organism=Escherichia coli, GI1788453, Length=402, Percent_Identity=24.3781094527363, Blast_Score=121, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019800
- InterPro:   IPR002772
- InterPro:   IPR001764
- InterPro:   IPR017853 [H]

Pfam domain/function: PF00933 Glyco_hydro_3; PF01915 Glyco_hydro_3_C [H]

EC number: 3.2.1.52

Molecular weight: Translated: 58590; Mature: 58590

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIEEMTLEEKIGQMLMIGFPSAFYDDHIRELVTSYKIGNVILFSRNVQDARQVMDLCT
CCHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHH
DIQKEAIENTGIPAFISIDQEGGMVTRIYRGATYFPGNMAIGATKDPENAYKVGEIAGRE
HHHHHHHHCCCCCEEEEEECCCCEEEEEECCCEECCCCEEECCCCCCHHHHHHHHHCCCE
LRTIGININFAPVMDVNNNPLNPVIGVRSYGENPEDVANFGINYIKGLQKEGVIATAKHF
EEEEEEEEEEEEEEECCCCCCCCEEECCCCCCCHHHHHHCCHHHHHCCCCCCEEEEECCC
PGHGDTTVDSHLDLPKVNHGKDRLYEVELYPFKKAVENGVDAIMTAHILFPALEDSKVPA
CCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHCCHHHHHHHHHHHCCCCCCCCCE
TLSYNILTRILREEFKFDGLVITDCMEMNAIAKYFGTQKAASMAIKAGADIVLVSHTKEL
EEEHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEECCCCC
QIKAFENIKEAVERGDISVERIDESVRRILKMKEKYGLFDRPYPEKDKFDTTVGIEKHRK
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHH
IAKAISLKSITVVKDEKKLIPLKTKNVLAISPETVPVSFVDDALMDRLSFARQFTSTFGG
HHHHHHHCEEEEEECCCCEECEECCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
VEETISVNPNNREISVILEKAKDKEVVVVGTYNANLNEGQVKLVNALLQVNKNVIVVALR
CCEEEEECCCCCEEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEEC
NPYDLQKFENVPTYVCAYEYTPLSVESVIGVLTGKYPAEGKLPVTI
CCCCHHHHCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKIEEMTLEEKIGQMLMIGFPSAFYDDHIRELVTSYKIGNVILFSRNVQDARQVMDLCT
CCHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHH
DIQKEAIENTGIPAFISIDQEGGMVTRIYRGATYFPGNMAIGATKDPENAYKVGEIAGRE
HHHHHHHHCCCCCEEEEEECCCCEEEEEECCCEECCCCEEECCCCCCHHHHHHHHHCCCE
LRTIGININFAPVMDVNNNPLNPVIGVRSYGENPEDVANFGINYIKGLQKEGVIATAKHF
EEEEEEEEEEEEEEECCCCCCCCEEECCCCCCCHHHHHHCCHHHHHCCCCCCEEEEECCC
PGHGDTTVDSHLDLPKVNHGKDRLYEVELYPFKKAVENGVDAIMTAHILFPALEDSKVPA
CCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHCCHHHHHHHHHHHCCCCCCCCCE
TLSYNILTRILREEFKFDGLVITDCMEMNAIAKYFGTQKAASMAIKAGADIVLVSHTKEL
EEEHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEECCCCC
QIKAFENIKEAVERGDISVERIDESVRRILKMKEKYGLFDRPYPEKDKFDTTVGIEKHRK
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHH
IAKAISLKSITVVKDEKKLIPLKTKNVLAISPETVPVSFVDDALMDRLSFARQFTSTFGG
HHHHHHHCEEEEEECCCCEECEECCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
VEETISVNPNNREISVILEKAKDKEVVVVGTYNANLNEGQVKLVNALLQVNKNVIVVALR
CCEEEEECCCCCEEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEEC
NPYDLQKFENVPTYVCAYEYTPLSVESVIGVLTGKYPAEGKLPVTI
CCCCHHHHCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8011666; 9274029; 9384377 [H]