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The map label for this gene is AceF [H]

Identifier: 20806714

GI number: 20806714

Start: 191694

End: 192938

Strand: Direct

Name: AceF [H]

Synonym: TTE0188

Alternate gene names: 20806714

Gene position: 191694-192938 (Clockwise)

Preceding gene: 20806713

Following gene: 20806715

Centisome position: 7.13

GC content: 34.7

Gene sequence:

>1245_bases
ATGGCAAATGTAAAACTTATGCCTAAGTTAGGTATGACTATGACAGCTGGCAAAGTAGTTAGATGGTTGAAAAAAGAAGG
TGAAAAAGTAGAAGCAGGAGAGCCTCTTTTGGAAATAGAAACTGACAAAGTTACAATGGAAGAAGAAGCAGGTTACACAG
GGACTCTTTTAAAAATTCTTGTAAGAGAGGGAGAAGAAGTTCCTATAAATCAGCCTATAGCTATAATAGGAGGAGAAGGA
GAAGACATAGAAGAGATACTAAAAACGTTAAAAGTCTCTGAGGGAGTTGAAGAAGAGAAAAAAGAAGAGAAGATAAAAGT
AAAAATAGAGGAGAAGCCAGAAGAGGTGAAAAGAGAAGAAATATCTAAACCAAGAGCTACACCGGCAGCAAGGAAAATAG
CAAGGGAACATGGGATAGACCTGTCAGAAGTAATAGGCTCTGGAGCTCACGGCAGAATTCATAGGAAAGATGTAGAAGAA
TATATTAGAAAGCGTACTGAAATAGTGCCTTCACAAGTAACAGTAGAACAAAAAGTAGAGAAAAAAGAAGAAATACCCTC
TTATAGAGTTATTCCTTTCACAGGAATGAGAAAAATAATAGCAGAAAAAATGCAAAAAAGTATAAATACAGCTCCTCATT
TTTATGTTACTATGGAAGTAAAGATGAGAGAAATTTTAAAATTGAGAGAGACATTAAATAGTAAATTGAAAGAGGATGAA
GCAAAAATTAGCCTTAATACCCTGCTTATGAAAGCTGCTGGAATAGCAATAAAAGATTATCCGATATTTAATTCTTATGT
AGAGGAAGGCCAGATAATTTTAAGGAATGAAATAAATATAGGATTGGCGGTAGCTTTGGATGAAGGACTAATTGTACCTG
TTATAAGGGAAGTAGACAAAAAAGGATTGAAAGAAATAGCAAGGGAAGAAAAAGCACTTATTCAGAAAGCAAGAGAAGGA
AAATTAACACCTGATGAGTATACTGGAGGAAGTTTCACAATCTCTAATTTAGGTATGTTTGATGTAGTAAGATTTGCAGC
TATAATTAATCCACCGGAAGTAGCAATTTTAGCTGTAGGGAAGATAAGAGAGATTCCCGTTGTAGAAGAAGGACAAATCG
AAATAGAGCCGATAATGGAAATGACTTTATCTTCAGATCATAGAGTAATAGACGGTGCTCTCGCGGCTAAATTTTTGAGA
AGGATAAAAGAAATTTTAGAAGATCCCTTACAATTTATGTTGTAA

Upstream 100 bases:

>100_bases
CCAATTCCTTACAATCCCAAGTTAGAGAGAGCGGCTGTACCTCAAGTAGAAGACATAATAGAGGCAGTAAAAGCTATATT
TGGCAAGGAGTGATTGAAGG

Downstream 100 bases:

>100_bases
GAAAAGGCAGGGATGTGAGTTTTATGTACACTTACATGAAAGGAATGCTTTACTGCCTTTACTGCAAAAAGGAGACTGAA
CATACGATAGTCTATGAAGG

Product: dihydrolipoamide acyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MANVKLMPKLGMTMTAGKVVRWLKKEGEKVEAGEPLLEIETDKVTMEEEAGYTGTLLKILVREGEEVPINQPIAIIGGEG
EDIEEILKTLKVSEGVEEEKKEEKIKVKIEEKPEEVKREEISKPRATPAARKIAREHGIDLSEVIGSGAHGRIHRKDVEE
YIRKRTEIVPSQVTVEQKVEKKEEIPSYRVIPFTGMRKIIAEKMQKSINTAPHFYVTMEVKMREILKLRETLNSKLKEDE
AKISLNTLLMKAAGIAIKDYPIFNSYVEEGQIILRNEINIGLAVALDEGLIVPVIREVDKKGLKEIAREEKALIQKAREG
KLTPDEYTGGSFTISNLGMFDVVRFAAIINPPEVAILAVGKIREIPVVEEGQIEIEPIMEMTLSSDHRVIDGALAAKFLR
RIKEILEDPLQFML

Sequences:

>Translated_414_residues
MANVKLMPKLGMTMTAGKVVRWLKKEGEKVEAGEPLLEIETDKVTMEEEAGYTGTLLKILVREGEEVPINQPIAIIGGEG
EDIEEILKTLKVSEGVEEEKKEEKIKVKIEEKPEEVKREEISKPRATPAARKIAREHGIDLSEVIGSGAHGRIHRKDVEE
YIRKRTEIVPSQVTVEQKVEKKEEIPSYRVIPFTGMRKIIAEKMQKSINTAPHFYVTMEVKMREILKLRETLNSKLKEDE
AKISLNTLLMKAAGIAIKDYPIFNSYVEEGQIILRNEINIGLAVALDEGLIVPVIREVDKKGLKEIAREEKALIQKAREG
KLTPDEYTGGSFTISNLGMFDVVRFAAIINPPEVAILAVGKIREIPVVEEGQIEIEPIMEMTLSSDHRVIDGALAAKFLR
RIKEILEDPLQFML
>Mature_413_residues
ANVKLMPKLGMTMTAGKVVRWLKKEGEKVEAGEPLLEIETDKVTMEEEAGYTGTLLKILVREGEEVPINQPIAIIGGEGE
DIEEILKTLKVSEGVEEEKKEEKIKVKIEEKPEEVKREEISKPRATPAARKIAREHGIDLSEVIGSGAHGRIHRKDVEEY
IRKRTEIVPSQVTVEQKVEKKEEIPSYRVIPFTGMRKIIAEKMQKSINTAPHFYVTMEVKMREILKLRETLNSKLKEDEA
KISLNTLLMKAAGIAIKDYPIFNSYVEEGQIILRNEINIGLAVALDEGLIVPVIREVDKKGLKEIAREEKALIQKAREGK
LTPDEYTGGSFTISNLGMFDVVRFAAIINPPEVAILAVGKIREIPVVEEGQIEIEPIMEMTLSSDHRVIDGALAAKFLRR
IKEILEDPLQFML

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=434, Percent_Identity=36.1751152073733, Blast_Score=256, Evalue=3e-68,
Organism=Homo sapiens, GI203098753, Length=448, Percent_Identity=36.1607142857143, Blast_Score=244, Evalue=1e-64,
Organism=Homo sapiens, GI203098816, Length=448, Percent_Identity=36.1607142857143, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI110671329, Length=413, Percent_Identity=32.4455205811138, Blast_Score=182, Evalue=6e-46,
Organism=Homo sapiens, GI19923748, Length=222, Percent_Identity=36.036036036036, Blast_Score=145, Evalue=8e-35,
Organism=Homo sapiens, GI260898739, Length=142, Percent_Identity=49.2957746478873, Blast_Score=138, Evalue=1e-32,
Organism=Escherichia coli, GI1786946, Length=412, Percent_Identity=33.9805825242718, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1786305, Length=405, Percent_Identity=33.5802469135802, Blast_Score=173, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17560088, Length=436, Percent_Identity=38.302752293578, Blast_Score=251, Evalue=7e-67,
Organism=Caenorhabditis elegans, GI25146366, Length=417, Percent_Identity=32.1342925659472, Blast_Score=171, Evalue=9e-43,
Organism=Caenorhabditis elegans, GI17538894, Length=301, Percent_Identity=34.5514950166113, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17537937, Length=409, Percent_Identity=28.6063569682152, Blast_Score=156, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6324258, Length=446, Percent_Identity=33.6322869955157, Blast_Score=242, Evalue=7e-65,
Organism=Saccharomyces cerevisiae, GI6320352, Length=419, Percent_Identity=29.1169451073986, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6321632, Length=297, Percent_Identity=28.2828282828283, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI20129315, Length=293, Percent_Identity=35.4948805460751, Blast_Score=193, Evalue=2e-49,
Organism=Drosophila melanogaster, GI24582497, Length=293, Percent_Identity=35.4948805460751, Blast_Score=193, Evalue=2e-49,
Organism=Drosophila melanogaster, GI18859875, Length=416, Percent_Identity=28.3653846153846, Blast_Score=178, Evalue=6e-45,
Organism=Drosophila melanogaster, GI24645909, Length=215, Percent_Identity=36.2790697674419, Blast_Score=144, Evalue=2e-34,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 46616; Mature: 46485

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVKLMPKLGMTMTAGKVVRWLKKEGEKVEAGEPLLEIETDKVTMEEEAGYTGTLLKIL
CCCCEECCCCCCCHHHHHHHHHHHHCCCEECCCCCEEEEECCCEEEHHCCCCHHHHHHHH
VREGEEVPINQPIAIIGGEGEDIEEILKTLKVSEGVEEEKKEEKIKVKIEEKPEEVKREE
HHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCHHHHHHHCEEEEEECCCHHHHHHHH
ISKPRATPAARKIAREHGIDLSEVIGSGAHGRIHRKDVEEYIRKRTEIVPSQVTVEQKVE
HCCCCCCHHHHHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
KKEEIPSYRVIPFTGMRKIIAEKMQKSINTAPHFYVTMEVKMREILKLRETLNSKLKEDE
HHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHH
AKISLNTLLMKAAGIAIKDYPIFNSYVEEGQIILRNEINIGLAVALDEGLIVPVIREVDK
HHHHHHHHHHHHCCCEEECCCCHHHHHHCCCEEEEECCCEEEEEEECCCCHHHHHHHHHH
KGLKEIAREEKALIQKAREGKLTPDEYTGGSFTISNLGMFDVVRFAAIINPPEVAILAVG
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCEEEEEEC
KIREIPVVEEGQIEIEPIMEMTLSSDHRVIDGALAAKFLRRIKEILEDPLQFML
CCCCCCCCCCCCEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ANVKLMPKLGMTMTAGKVVRWLKKEGEKVEAGEPLLEIETDKVTMEEEAGYTGTLLKIL
CCCEECCCCCCCHHHHHHHHHHHHCCCEECCCCCEEEEECCCEEEHHCCCCHHHHHHHH
VREGEEVPINQPIAIIGGEGEDIEEILKTLKVSEGVEEEKKEEKIKVKIEEKPEEVKREE
HHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCHHHHHHHCEEEEEECCCHHHHHHHH
ISKPRATPAARKIAREHGIDLSEVIGSGAHGRIHRKDVEEYIRKRTEIVPSQVTVEQKVE
HCCCCCCHHHHHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
KKEEIPSYRVIPFTGMRKIIAEKMQKSINTAPHFYVTMEVKMREILKLRETLNSKLKEDE
HHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHH
AKISLNTLLMKAAGIAIKDYPIFNSYVEEGQIILRNEINIGLAVALDEGLIVPVIREVDK
HHHHHHHHHHHHCCCEEECCCCHHHHHHCCCEEEEECCCEEEEEEECCCCHHHHHHHHHH
KGLKEIAREEKALIQKAREGKLTPDEYTGGSFTISNLGMFDVVRFAAIINPPEVAILAVG
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCEEEEEEC
KIREIPVVEEGQIEIEPIMEMTLSSDHRVIDGALAAKFLRRIKEILEDPLQFML
CCCCCCCCCCCCEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA