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The map label for this gene is HolB [H]

Identifier: 20806626

GI number: 20806626

Start: 98846

End: 99808

Strand: Direct

Name: HolB [H]

Synonym: TTE0097

Alternate gene names: 20806626

Gene position: 98846-99808 (Clockwise)

Preceding gene: 20806625

Following gene: 20806627

Centisome position: 3.68

GC content: 33.33

Gene sequence:

>963_bases
GTGTATGGCCACGAAGAGATTCTAAAAGTATTTCGCAATATAATAAATCAGAATAAAATTTCTAATGCCTACTTATTTGT
TGGGGAAGAAGGTTTAGGAAAGAGATTTTTGGCTGAATATTTTGCAATGATGGTAAACTGCAAAGGTAATGATAAGCCTT
GTTTTAAGTGTACCTCTTGTGTAAAAATGCTTAAGAAAAGTCATCCAGATGTGTTTATAATAGAACCAGAGGGAGATTCT
ATAAAAGTAGAAACTGTAAGGTATATAAACGCTGAAATAAATATAAAGCCTTATGAGTCTTTTAAAAAGGTATTTATAAT
TGACAGGGCTGATAAAATGACTCCTGCAGCTCAGAACGCTTTTCTTAAAACCTTAGAAGAACCGCCTTTATACGGCCTTT
TCATACTAATCTCACCTCAAATAGAAGCTCTTTTGCCTACAGTAGTGTCCCGCTGCAATGTGGTAAGATTTAAAAGAGAA
AGAAAAGAAGTGATTAAAGAATACCTGATTGGTGAAAAAGGCGTCAGCGAAGAAGAGGCAGAAGTTCTGGCACATCTCGC
GGATGGCAATTTTGGTGAGGCGGACAAACTGGTGGACGAAAATTATAAAAAGCTTCGCAGCAATGTGCTTTCTGAGATTG
AGAAAATTTTTGAGAAAAAAGGTTTTGAAGTGCTTAATGAGTACAGCTTTTTTGAGGAAAATAAGGAACAGATTGAGGAG
GTTTTAAAAATATTTCTCTCTTATTTAAGAGATGTGTTGGTCTTTAAAACAACCAAAGATTTGAAATTTATAAAGAATGT
GGATTATATAGATTCTATAGAAAAATTGGAATCTAAGTTGACCTTTAAGAGATTAAATAATATAATCAATAGAATAGAGC
AGTTTAATTCCCAGCTTAAAAGCAATGTAAACTATCAGTTGGCTGTAGAGAATTTACTTTTAGATATTTTAGGGGGTCAA
TAA

Upstream 100 bases:

>100_bases
TTTCTGAAAGAGTCTAAAAAGTTAGAACTCCTAAAAATGATTGATGATATAAGGGGTTTGTTAATAGACATTTACTCGTA
AGGTGATGAAGTTGCTTAGG

Downstream 100 bases:

>100_bases
CGTTGGTTACAGTCGTAGGAGTTAGATTTAAGAAGGCAGGCAAGATATACTATTTTGACCCGGGAGATTTGCCTATAAAA
GTTGGCGATAAGGTGATTGT

Product: DNA replication ATPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MYGHEEILKVFRNIINQNKISNAYLFVGEEGLGKRFLAEYFAMMVNCKGNDKPCFKCTSCVKMLKKSHPDVFIIEPEGDS
IKVETVRYINAEINIKPYESFKKVFIIDRADKMTPAAQNAFLKTLEEPPLYGLFILISPQIEALLPTVVSRCNVVRFKRE
RKEVIKEYLIGEKGVSEEEAEVLAHLADGNFGEADKLVDENYKKLRSNVLSEIEKIFEKKGFEVLNEYSFFEENKEQIEE
VLKIFLSYLRDVLVFKTTKDLKFIKNVDYIDSIEKLESKLTFKRLNNIINRIEQFNSQLKSNVNYQLAVENLLLDILGGQ

Sequences:

>Translated_320_residues
MYGHEEILKVFRNIINQNKISNAYLFVGEEGLGKRFLAEYFAMMVNCKGNDKPCFKCTSCVKMLKKSHPDVFIIEPEGDS
IKVETVRYINAEINIKPYESFKKVFIIDRADKMTPAAQNAFLKTLEEPPLYGLFILISPQIEALLPTVVSRCNVVRFKRE
RKEVIKEYLIGEKGVSEEEAEVLAHLADGNFGEADKLVDENYKKLRSNVLSEIEKIFEKKGFEVLNEYSFFEENKEQIEE
VLKIFLSYLRDVLVFKTTKDLKFIKNVDYIDSIEKLESKLTFKRLNNIINRIEQFNSQLKSNVNYQLAVENLLLDILGGQ
>Mature_320_residues
MYGHEEILKVFRNIINQNKISNAYLFVGEEGLGKRFLAEYFAMMVNCKGNDKPCFKCTSCVKMLKKSHPDVFIIEPEGDS
IKVETVRYINAEINIKPYESFKKVFIIDRADKMTPAAQNAFLKTLEEPPLYGLFILISPQIEALLPTVVSRCNVVRFKRE
RKEVIKEYLIGEKGVSEEEAEVLAHLADGNFGEADKLVDENYKKLRSNVLSEIEKIFEKKGFEVLNEYSFFEENKEQIEE
VLKIFLSYLRDVLVFKTTKDLKFIKNVDYIDSIEKLESKLTFKRLNNIINRIEQFNSQLKSNVNYQLAVENLLLDILGGQ

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=205, Percent_Identity=30.2439024390244, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787341, Length=302, Percent_Identity=26.8211920529801, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004622 [H]

Pfam domain/function: NA

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 37085; Mature: 37085

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYGHEEILKVFRNIINQNKISNAYLFVGEEGLGKRFLAEYFAMMVNCKGNDKPCFKCTSC
CCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHH
VKMLKKSHPDVFIIEPEGDSIKVETVRYINAEINIKPYESFKKVFIIDRADKMTPAAQNA
HHHHHCCCCCEEEEECCCCEEEEEEEEEEECEEEECCHHHHCEEEEEECCCCCCHHHHHH
FLKTLEEPPLYGLFILISPQIEALLPTVVSRCNVVRFKRERKEVIKEYLIGEKGVSEEEA
HHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
EVLAHLADGNFGEADKLVDENYKKLRSNVLSEIEKIFEKKGFEVLNEYSFFEENKEQIEE
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHH
VLKIFLSYLRDVLVFKTTKDLKFIKNVDYIDSIEKLESKLTFKRLNNIINRIEQFNSQLK
HHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SNVNYQLAVENLLLDILGGQ
HCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYGHEEILKVFRNIINQNKISNAYLFVGEEGLGKRFLAEYFAMMVNCKGNDKPCFKCTSC
CCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHH
VKMLKKSHPDVFIIEPEGDSIKVETVRYINAEINIKPYESFKKVFIIDRADKMTPAAQNA
HHHHHCCCCCEEEEECCCCEEEEEEEEEEECEEEECCHHHHCEEEEEECCCCCCHHHHHH
FLKTLEEPPLYGLFILISPQIEALLPTVVSRCNVVRFKRERKEVIKEYLIGEKGVSEEEA
HHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
EVLAHLADGNFGEADKLVDENYKKLRSNVLSEIEKIFEKKGFEVLNEYSFFEENKEQIEE
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHH
VLKIFLSYLRDVLVFKTTKDLKFIKNVDYIDSIEKLESKLTFKRLNNIINRIEQFNSQLK
HHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SNVNYQLAVENLLLDILGGQ
HCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]