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The map label for this gene is PurR [H]

Identifier: 20806586

GI number: 20806586

Start: 60661

End: 61719

Strand: Direct

Name: PurR [H]

Synonym: TTE0053

Alternate gene names: 20806586

Gene position: 60661-61719 (Clockwise)

Preceding gene: 20806584

Following gene: 20806587

Centisome position: 2.26

GC content: 35.51

Gene sequence:

>1059_bases
ATGAGAAAACGATTATCAGAGGTGTTGAGCTTGACCACCACAAGACGCAAAAGAGTCACAATGAAGGATATAGCAGAGAA
ATTAAATTTATCAATTAACGCTGTATCTTTAGCTCTGAATGACAAGGTAGGGGTGAGCGAAGAGACAAGGAAGTTAGTTA
TAAAAACTGCAGAAGAGATGGGGTATTTTGAGGAAAATCCATCGTTTTTATCAAAAAGCCATTTAAAAAACATATGTCTC
ATAATAGAAGAGAGAAATTTTAAAGATACTAATTTTTACACTAAGGTCATATGGGGAATTGAAAATGAAGCAAAGGCTAA
TGGATACGACGTTTTAGTTAATTTTATGAAAAAAGATTTATTTGAAATTCCATCTTGCATAGAAAACAGGAAGGTTTCGG
GGATTTTGGTAGTGGGGACTATAAAAGACGAATTTCTTGCAGAAATTTTAAGCTACAATATTCCGACGGTTTTAGTAGAC
CACGCTTCTTTTATGTTTAGCACTGATGCCGTTCTTACACAGAATATTCCGGGGAGCTATAGAGGGACGAAGTACTTAAT
AGAAAAAGGGCATAAAAAAATTGGTTTTTTTGGAGAAAAAGACTTTTCTTTAAGCTTTAGAGAAAGATGGCTGGGATTTA
AGGAAGCCATGAGAGACGCTGGTTTACCTGTAAATGAAGACTACTGCGTTACTGAGGATGTTGAAAGGCACGTGCTTAGT
AAAAATTATGAGGAGGTTGCAAATAGATTAAAAAAATTAAAGGAATTTCCTACAGCTTGGGTTTGCAGTAATGACAGCAA
TGCTATAACTTTGTATAATGCCCTAAATATAATGGGTATAAAGGTTCCTAAAGATGTGTCTATTGTTGGCTTTGATGATA
TAGACATGTGCAATATTGTGACGCCGCCTTTGACTACTATACACATAAACAAAGAGCTTATGGGTATAAAAGCTGTAAAA
AGGCTTTTGTGGCGCATGGACAATCCTAAAGAATTCCATGACCACATAAGAATGGAGGTGGGATTTGTAGAAAGACAATC
TGTTAGAGAATTGCTGTAA

Upstream 100 bases:

>100_bases
TGACAAATTAATGGATGATTTCGGATAAATTCAAGTAAAATGTCGAATAATTAAAGTAAATTTTACTTGATAATTAAATT
TAAATGGAGTATAATTTTAT

Downstream 100 bases:

>100_bases
TTTAAATTCAATTAGGAAAGAAGAGGGGGAAAAACAATGAATAGCGTGCTTAAAAAATTTATTGCGATTTTTGTAATTGT
TTTCTTTACAGCTGGGATTT

Product: transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MRKRLSEVLSLTTTRRKRVTMKDIAEKLNLSINAVSLALNDKVGVSEETRKLVIKTAEEMGYFEENPSFLSKSHLKNICL
IIEERNFKDTNFYTKVIWGIENEAKANGYDVLVNFMKKDLFEIPSCIENRKVSGILVVGTIKDEFLAEILSYNIPTVLVD
HASFMFSTDAVLTQNIPGSYRGTKYLIEKGHKKIGFFGEKDFSLSFRERWLGFKEAMRDAGLPVNEDYCVTEDVERHVLS
KNYEEVANRLKKLKEFPTAWVCSNDSNAITLYNALNIMGIKVPKDVSIVGFDDIDMCNIVTPPLTTIHINKELMGIKAVK
RLLWRMDNPKEFHDHIRMEVGFVERQSVRELL

Sequences:

>Translated_352_residues
MRKRLSEVLSLTTTRRKRVTMKDIAEKLNLSINAVSLALNDKVGVSEETRKLVIKTAEEMGYFEENPSFLSKSHLKNICL
IIEERNFKDTNFYTKVIWGIENEAKANGYDVLVNFMKKDLFEIPSCIENRKVSGILVVGTIKDEFLAEILSYNIPTVLVD
HASFMFSTDAVLTQNIPGSYRGTKYLIEKGHKKIGFFGEKDFSLSFRERWLGFKEAMRDAGLPVNEDYCVTEDVERHVLS
KNYEEVANRLKKLKEFPTAWVCSNDSNAITLYNALNIMGIKVPKDVSIVGFDDIDMCNIVTPPLTTIHINKELMGIKAVK
RLLWRMDNPKEFHDHIRMEVGFVERQSVRELL
>Mature_352_residues
MRKRLSEVLSLTTTRRKRVTMKDIAEKLNLSINAVSLALNDKVGVSEETRKLVIKTAEEMGYFEENPSFLSKSHLKNICL
IIEERNFKDTNFYTKVIWGIENEAKANGYDVLVNFMKKDLFEIPSCIENRKVSGILVVGTIKDEFLAEILSYNIPTVLVD
HASFMFSTDAVLTQNIPGSYRGTKYLIEKGHKKIGFFGEKDFSLSFRERWLGFKEAMRDAGLPVNEDYCVTEDVERHVLS
KNYEEVANRLKKLKEFPTAWVCSNDSNAITLYNALNIMGIKVPKDVSIVGFDDIDMCNIVTPPLTTIHINKELMGIKAVK
RLLWRMDNPKEFHDHIRMEVGFVERQSVRELL

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=345, Percent_Identity=30.4347826086957, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1790194, Length=336, Percent_Identity=29.4642857142857, Blast_Score=137, Evalue=9e-34,
Organism=Escherichia coli, GI1790369, Length=342, Percent_Identity=30.4093567251462, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1789202, Length=294, Percent_Identity=24.8299319727891, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787580, Length=319, Percent_Identity=25.0783699059561, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1786540, Length=344, Percent_Identity=24.7093023255814, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1789456, Length=309, Percent_Identity=26.2135922330097, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789068, Length=328, Percent_Identity=25.3048780487805, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787906, Length=332, Percent_Identity=24.3975903614458, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI48994940, Length=328, Percent_Identity=26.219512195122, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788474, Length=295, Percent_Identity=25.0847457627119, Blast_Score=78, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 40431; Mature: 40431

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKRLSEVLSLTTTRRKRVTMKDIAEKLNLSINAVSLALNDKVGVSEETRKLVIKTAEEM
CHHHHHHHHHHHHHHHHCCCHHHHHHHHCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHC
GYFEENPSFLSKSHLKNICLIIEERNFKDTNFYTKVIWGIENEAKANGYDVLVNFMKKDL
CCCCCCCHHHHHHHHCCEEEEEECCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHH
FEIPSCIENRKVSGILVVGTIKDEFLAEILSYNIPTVLVDHASFMFSTDAVLTQNIPGSY
HHHHHHHHCCCCCEEEEEECCCHHHHHHHHHCCCCEEEEECHHHHEECCCCEECCCCCCC
RGTKYLIEKGHKKIGFFGEKDFSLSFRERWLGFKEAMRDAGLPVNEDYCVTEDVERHVLS
CCHHHHHHCCCCEECCCCCCCCCHHHHHHHCCHHHHHHHCCCCCCCCCCCCHHHHHHHHC
KNYEEVANRLKKLKEFPTAWVCSNDSNAITLYNALNIMGIKVPKDVSIVGFDDIDMCNIV
CCHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEEEEEEEEECCCCEEEEECCCCCCCCCC
TPPLTTIHINKELMGIKAVKRLLWRMDNPKEFHDHIRMEVGFVERQSVRELL
CCCCEEEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHC
>Mature Secondary Structure
MRKRLSEVLSLTTTRRKRVTMKDIAEKLNLSINAVSLALNDKVGVSEETRKLVIKTAEEM
CHHHHHHHHHHHHHHHHCCCHHHHHHHHCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHC
GYFEENPSFLSKSHLKNICLIIEERNFKDTNFYTKVIWGIENEAKANGYDVLVNFMKKDL
CCCCCCCHHHHHHHHCCEEEEEECCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHH
FEIPSCIENRKVSGILVVGTIKDEFLAEILSYNIPTVLVDHASFMFSTDAVLTQNIPGSY
HHHHHHHHCCCCCEEEEEECCCHHHHHHHHHCCCCEEEEECHHHHEECCCCEECCCCCCC
RGTKYLIEKGHKKIGFFGEKDFSLSFRERWLGFKEAMRDAGLPVNEDYCVTEDVERHVLS
CCHHHHHHCCCCEECCCCCCCCCHHHHHHHCCHHHHHHHCCCCCCCCCCCCHHHHHHHHC
KNYEEVANRLKKLKEFPTAWVCSNDSNAITLYNALNIMGIKVPKDVSIVGFDDIDMCNIV
CCHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEEEEEEEEECCCCEEEEECCCCCCCCCC
TPPLTTIHINKELMGIKAVKRLLWRMDNPKEFHDHIRMEVGFVERQSVRELL
CCCCEEEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]