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The map label for this gene is RsuA [H]

Identifier: 20806585

GI number: 20806585

Start: 59729

End: 60451

Strand: Reverse

Name: RsuA [H]

Synonym: TTE0052

Alternate gene names: 20806585

Gene position: 60451-59729 (Counterclockwise)

Preceding gene: 20806605

Following gene: 20806582

Centisome position: 2.25

GC content: 35.27

Gene sequence:

>723_bases
ATGGCAAAAATGAGATTAGACAAATTGCTATCAAATATGGGAATAGGTACAAGAAAAGAAGTGAAAAAGTACATAAAAGA
CGGTTTAGTAGAGGTAAATGGAGAGATAGTATTAGACCCTTCTAAAACCGTAGATACAGAAAAAGATGAGGTCTTATTCG
ATGGGGAAAAAGTAGAATACAAAGAATTTATATACGTGATGATGAATAAACCCCAAGGAGTCATCAGTGCAACCCACGAC
AAATCCGAAGAAACCGTGATAGACCTCCTTCCAGAGGAAATTGCTTTAAGAAAAGTCTTTCCTGTAGGAAGGCTTGATAA
AGACACGGAAGGCCTACTTATAATAACAAACGACGGAGAGCTTGCCCACAAACTCCTTTCTCCAAAGAAAAAAGTGGTAA
AAAAATACTATGCCGAAGTATTAGGAAAAATAACTGAAAAAGACGCAGAAAAATTTAAAGAAGGAGTAATACTTGAAGAT
GGATACAGGACAATGCCATCGCAGTTAGAAATAATTGAATCTTCCGATATTTCCAAAGTGTATGTTTACCTCACAGAAGG
AAAATACCACCAGGTAAAGAGAATGTTTGAAGCAATAGGAAAGAAAGTATTATACCTTAAAAGGCTTTGTATGGGAAAGC
TCCAGTTAGATGAAAATTTAGAGCCTGGAGAATGGAGAGAATTGACAGAAGAAGAAGTAAATTTATTAAAGGAAACTATA
TAA

Upstream 100 bases:

>100_bases
TTAAGCCAGTTTAAATGCTGGAAATTTACTTGAAAAGTAAAGTTAATTAAAGAAAAGAATATGTGGTATAATTAAAAAGC
AACCAAAAGGAGGGTTTGTT

Downstream 100 bases:

>100_bases
AGCCCCCGTCTACTGTAATCACTTGACCTGTTATAAAATTCGCCTTATCAGAAAGCAAAAACAAGACTGCGTTTGCCACG
TCCTCAGGTTTTCCTATGCT

Product: 16S rRNA uridine-516 pseudouridylate synthase family protein

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MAKMRLDKLLSNMGIGTRKEVKKYIKDGLVEVNGEIVLDPSKTVDTEKDEVLFDGEKVEYKEFIYVMMNKPQGVISATHD
KSEETVIDLLPEEIALRKVFPVGRLDKDTEGLLIITNDGELAHKLLSPKKKVVKKYYAEVLGKITEKDAEKFKEGVILED
GYRTMPSQLEIIESSDISKVYVYLTEGKYHQVKRMFEAIGKKVLYLKRLCMGKLQLDENLEPGEWRELTEEEVNLLKETI

Sequences:

>Translated_240_residues
MAKMRLDKLLSNMGIGTRKEVKKYIKDGLVEVNGEIVLDPSKTVDTEKDEVLFDGEKVEYKEFIYVMMNKPQGVISATHD
KSEETVIDLLPEEIALRKVFPVGRLDKDTEGLLIITNDGELAHKLLSPKKKVVKKYYAEVLGKITEKDAEKFKEGVILED
GYRTMPSQLEIIESSDISKVYVYLTEGKYHQVKRMFEAIGKKVLYLKRLCMGKLQLDENLEPGEWRELTEEEVNLLKETI
>Mature_239_residues
AKMRLDKLLSNMGIGTRKEVKKYIKDGLVEVNGEIVLDPSKTVDTEKDEVLFDGEKVEYKEFIYVMMNKPQGVISATHDK
SEETVIDLLPEEIALRKVFPVGRLDKDTEGLLIITNDGELAHKLLSPKKKVVKKYYAEVLGKITEKDAEKFKEGVILEDG
YRTMPSQLEIIESSDISKVYVYLTEGKYHQVKRMFEAIGKKVLYLKRLCMGKLQLDENLEPGEWRELTEEEVNLLKETI

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=232, Percent_Identity=38.3620689655172, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1787524, Length=246, Percent_Identity=33.3333333333333, Blast_Score=121, Evalue=4e-29,
Organism=Escherichia coli, GI1790453, Length=246, Percent_Identity=31.7073170731707, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI87081838, Length=181, Percent_Identity=35.9116022099448, Blast_Score=103, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 27561; Mature: 27430

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKMRLDKLLSNMGIGTRKEVKKYIKDGLVEVNGEIVLDPSKTVDTEKDEVLFDGEKVEY
CCCHHHHHHHHHCCCCCHHHHHHHHHCCCEEECCEEEECCCCCCCCCCCCEEECCCCCCH
KEFIYVMMNKPQGVISATHDKSEETVIDLLPEEIALRKVFPVGRLDKDTEGLLIITNDGE
HHEEEEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHH
LAHKLLSPKKKVVKKYYAEVLGKITEKDAEKFKEGVILEDGYRTMPSQLEIIESSDISKV
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHCCCCCCEE
YVYLTEGKYHQVKRMFEAIGKKVLYLKRLCMGKLQLDENLEPGEWRELTEEEVNLLKETI
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKMRLDKLLSNMGIGTRKEVKKYIKDGLVEVNGEIVLDPSKTVDTEKDEVLFDGEKVEY
CCHHHHHHHHHCCCCCHHHHHHHHHCCCEEECCEEEECCCCCCCCCCCCEEECCCCCCH
KEFIYVMMNKPQGVISATHDKSEETVIDLLPEEIALRKVFPVGRLDKDTEGLLIITNDGE
HHEEEEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHH
LAHKLLSPKKKVVKKYYAEVLGKITEKDAEKFKEGVILEDGYRTMPSQLEIIESSDISKV
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHCCCCCCEE
YVYLTEGKYHQVKRMFEAIGKKVLYLKRLCMGKLQLDENLEPGEWRELTEEEVNLLKETI
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]