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The map label for this gene is rnz

Identifier: 203284649

GI number: 203284649

Start: 813429

End: 814388

Strand: Reverse

Name: rnz

Synonym: BDU_759

Alternate gene names: 203284649

Gene position: 814388-813429 (Counterclockwise)

Preceding gene: 203284650

Following gene: 203284648

Centisome position: 87.41

GC content: 26.25

Gene sequence:

>960_bases
TTGAATTTTAATATTAATATTCTTGGCACAGGAGGCACAAGACCATTACACAACAGATATTTAACATCTGTTTTAATTGA
ATACCACGGAGAAAGTATTCTTTTTGACTGTGGAGAAGCTACACAAATGTCTCTTAGGAAACAAAAAATATCATGGCAAA
AAATTAAAATGATTTGTATCACACATTTACACGCCGATCATATCACAGGACTACTTGGAATAGTAATGTTAATGGCACAA
AGTGGTGATACAAGAAAAGAACCTTTAACCATTATTGGCCCTATAGGTATCAAAAAATATTTAGAAACAAATATTGAACT
CTTAAGAGTACATAAAAATTATCAAATAATATATAAAGAAATAATAATTAATAAGACAGAACCTGTACTATATGAAGATA
AAAGAAAAAGAATTGAATATATAAAACTTAAACATTCAATAGATTGTATTGGATATTTATTTATAGAAAAAGATAAACCT
GGTAAATTTGACATACAAAAAGCAGAAAGCCTAAATATACCAAAAGGACCTATTAGAAAAAAACTACAAGAAGGATATGA
AGTAATGCTGAATGGAAGAAAAATAGTTCCTTCTGAAATATTAGGAGAAACAAAAAAAGGACTCAAATTTGCATATATTA
CAGATACGGCTTACTTTGAAGAATTAAGCACATATATTCAAAATTTCAATTTAGTAATTATTGAGAGTACATTTAAAGAT
GACTTAAAAGAAGAAGCTAAAAAAAAATTACATCTAACAGCAAAATTAGCAGCACAAATTACAAAAAAAGCAAAAGTATA
TCAAACTGGACTTATACATTTTAGTGAAAGATATACTTTAAATAAAGACCTATACGAATTATTAAATGAAGCACAACAAG
AATATCCAAATGGAAACATATTCTTAGCAAAAGATGGTATGAAACTTAAAGCAAATAAAGATAAATTTATTATAAAATAA

Upstream 100 bases:

>100_bases
AAAAAAATGCAAAATACCAATATAATCATGCACATTTTAAAAGCCAATTTTAACATGTATATCCATAATTTACTAAACAA
AAAGAATTGGGAGATTATTT

Downstream 100 bases:

>100_bases
TAATATCTAAGGAGATAACAAATGATCAATGTAAACAACATTACTAAAACATATGGTTCATTTACAGCTCTCTTAAATGT
TAGCTTCAAAGTTAACGAAG

Product: ribonuclease Z

Products: NA

Alternate protein names: RNase Z; tRNA 3 endonuclease; tRNase Z

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MNFNINILGTGGTRPLHNRYLTSVLIEYHGESILFDCGEATQMSLRKQKISWQKIKMICITHLHADHITGLLGIVMLMAQ
SGDTRKEPLTIIGPIGIKKYLETNIELLRVHKNYQIIYKEIIINKTEPVLYEDKRKRIEYIKLKHSIDCIGYLFIEKDKP
GKFDIQKAESLNIPKGPIRKKLQEGYEVMLNGRKIVPSEILGETKKGLKFAYITDTAYFEELSTYIQNFNLVIIESTFKD
DLKEEAKKKLHLTAKLAAQITKKAKVYQTGLIHFSERYTLNKDLYELLNEAQQEYPNGNIFLAKDGMKLKANKDKFIIK

Sequences:

>Translated_319_residues
MNFNINILGTGGTRPLHNRYLTSVLIEYHGESILFDCGEATQMSLRKQKISWQKIKMICITHLHADHITGLLGIVMLMAQ
SGDTRKEPLTIIGPIGIKKYLETNIELLRVHKNYQIIYKEIIINKTEPVLYEDKRKRIEYIKLKHSIDCIGYLFIEKDKP
GKFDIQKAESLNIPKGPIRKKLQEGYEVMLNGRKIVPSEILGETKKGLKFAYITDTAYFEELSTYIQNFNLVIIESTFKD
DLKEEAKKKLHLTAKLAAQITKKAKVYQTGLIHFSERYTLNKDLYELLNEAQQEYPNGNIFLAKDGMKLKANKDKFIIK
>Mature_319_residues
MNFNINILGTGGTRPLHNRYLTSVLIEYHGESILFDCGEATQMSLRKQKISWQKIKMICITHLHADHITGLLGIVMLMAQ
SGDTRKEPLTIIGPIGIKKYLETNIELLRVHKNYQIIYKEIIINKTEPVLYEDKRKRIEYIKLKHSIDCIGYLFIEKDKP
GKFDIQKAESLNIPKGPIRKKLQEGYEVMLNGRKIVPSEILGETKKGLKFAYITDTAYFEELSTYIQNFNLVIIESTFKD
DLKEEAKKKLHLTAKLAAQITKKAKVYQTGLIHFSERYTLNKDLYELLNEAQQEYPNGNIFLAKDGMKLKANKDKFIIK

Specific function: Zinc phosphodiesterase, which displays some tRNA 3'- processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA

COG id: COG1234

COG function: function code R; Metal-dependent hydrolases of the beta-lactamase superfamily III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase Z family

Homologues:

Organism=Homo sapiens, GI8922122, Length=318, Percent_Identity=29.559748427673, Blast_Score=149, Evalue=2e-36,
Organism=Escherichia coli, GI87082078, Length=294, Percent_Identity=28.9115646258503, Blast_Score=139, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNZ_BORDL (B5RMU7)

Other databases:

- EMBL:   CP000976
- RefSeq:   YP_002222389.1
- ProteinModelPortal:   B5RMU7
- EnsemblBacteria:   EBBORT00000001291
- GeneID:   6917985
- GenomeReviews:   CP000976_GR
- KEGG:   bdu:BDU_759
- GeneTree:   EBGT00050000006811
- HOGENOM:   HBG648406
- OMA:   HSTSTMA
- ProtClustDB:   PRK00055
- HAMAP:   MF_01818
- InterPro:   IPR001279
- InterPro:   IPR013471
- SMART:   SM00849
- TIGRFAMs:   TIGR02651

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.26.11

Molecular weight: Translated: 36857; Mature: 36857

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFNINILGTGGTRPLHNRYLTSVLIEYHGESILFDCGEATQMSLRKQKISWQKIKMICI
CCEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCHHHHHHHHH
THLHADHITGLLGIVMLMAQSGDTRKEPLTIIGPIGIKKYLETNIELLRVHKNYQIIYKE
HHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHCCEEEEEEECCHHEEEEH
IIINKTEPVLYEDKRKRIEYIKLKHSIDCIGYLFIEKDKPGKFDIQKAESLNIPKGPIRK
HHHCCCCCCEEHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHH
KLQEGYEVMLNGRKIVPSEILGETKKGLKFAYITDTAYFEELSTYIQNFNLVIIESTFKD
HHHCCHHEEECCCEECCHHHHCHHHHCCEEEEEECHHHHHHHHHHHHCCCEEEEECHHHH
DLKEEAKKKLHLTAKLAAQITKKAKVYQTGLIHFSERYTLNKDLYELLNEAQQEYPNGNI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCEECCHHHHHHHHHHHHHCCCCCE
FLAKDGMKLKANKDKFIIK
EEEECCEEEECCCCCEEEC
>Mature Secondary Structure
MNFNINILGTGGTRPLHNRYLTSVLIEYHGESILFDCGEATQMSLRKQKISWQKIKMICI
CCEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCHHHHHHHHH
THLHADHITGLLGIVMLMAQSGDTRKEPLTIIGPIGIKKYLETNIELLRVHKNYQIIYKE
HHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHCCEEEEEEECCHHEEEEH
IIINKTEPVLYEDKRKRIEYIKLKHSIDCIGYLFIEKDKPGKFDIQKAESLNIPKGPIRK
HHHCCCCCCEEHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHH
KLQEGYEVMLNGRKIVPSEILGETKKGLKFAYITDTAYFEELSTYIQNFNLVIIESTFKD
HHHCCHHEEECCCEECCHHHHCHHHHCCEEEEEECHHHHHHHHHHHHCCCEEEEECHHHH
DLKEEAKKKLHLTAKLAAQITKKAKVYQTGLIHFSERYTLNKDLYELLNEAQQEYPNGNI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCEECCHHHHHHHHHHHHHCCCCCE
FLAKDGMKLKANKDKFIIK
EEEECCEEEECCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA