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The map label for this gene is gph [C]

Identifier: 203284574

GI number: 203284574

Start: 725691

End: 726377

Strand: Reverse

Name: gph [C]

Synonym: BDU_679

Alternate gene names: 203284574

Gene position: 726377-725691 (Counterclockwise)

Preceding gene: 203284580

Following gene: 203284573

Centisome position: 77.96

GC content: 28.97

Gene sequence:

>687_bases
ATGACTTTCAAGGGGATTTACAATATGAAAATCAAAGCTTGCATTTTTGACATGGATGGAACTTTAATAAACAGCATTAT
GGATATTGCATCATCAATGAACCTTGCTTTAAAAAAATTAGGATATAGAGAAATTAAAACAGAAGAATTTAATACCCTAG
TTGGAAGAGGATATTCCAAATTAATAGAAAACACCCTAAAACATCTAAATATAGATCTAAATGACAAGCACCTTAATGAC
AATCTTTATCAAGAATTTGTAAAAGCATACAATCAAAATTTATATTTTCACACAAAAACATATGATGGAATACCAGAACT
TTTGAAAAAACTAAACTCACTTAAAATCCCTATTGGAATTTTAAGTAATAAAAACCATGAAGAATTACTAATAATCACAA
AAGACATATTTAAAGATATCAAATTTTTTGAAGTCAGAGGTTACTCATCAAAATTCGAAGCAAAACCAGATCCTGAAAAT
GCCCTTGATATGATTATAGAACTCAATCTTATGCCACAAGAAATAGCATATATTGGTGATAGTGACGTTGATATGTTAAC
TGCTCAAAATGCTGGTTTTTTGCCCATAGGAGTCTCATGGGGATTTAGAACAATACAAGAATTAAAAGAAAACGGAGCAA
AATACATATTAAATAATCCATTTGAACTCCTAGACATACTCAAATGA

Upstream 100 bases:

>100_bases
GGAATCTTTAGAAAAAAAACAAAAATAATGTAAAATATTATAAATACTATAATAAAATAAACAAAAAATAGAAATAATGA
TAAAATGAAGCAAATGATAT

Downstream 100 bases:

>100_bases
ACCTAAAAATATATTTTCCACATCTTTACCACTACTTATTTAATCATGAAAGTATAAAAGGATTGTCGGTTTTTGAAAAA
GAAGTTGAAATAATCAAATA

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MTFKGIYNMKIKACIFDMDGTLINSIMDIASSMNLALKKLGYREIKTEEFNTLVGRGYSKLIENTLKHLNIDLNDKHLND
NLYQEFVKAYNQNLYFHTKTYDGIPELLKKLNSLKIPIGILSNKNHEELLIITKDIFKDIKFFEVRGYSSKFEAKPDPEN
ALDMIIELNLMPQEIAYIGDSDVDMLTAQNAGFLPIGVSWGFRTIQELKENGAKYILNNPFELLDILK

Sequences:

>Translated_228_residues
MTFKGIYNMKIKACIFDMDGTLINSIMDIASSMNLALKKLGYREIKTEEFNTLVGRGYSKLIENTLKHLNIDLNDKHLND
NLYQEFVKAYNQNLYFHTKTYDGIPELLKKLNSLKIPIGILSNKNHEELLIITKDIFKDIKFFEVRGYSSKFEAKPDPEN
ALDMIIELNLMPQEIAYIGDSDVDMLTAQNAGFLPIGVSWGFRTIQELKENGAKYILNNPFELLDILK
>Mature_227_residues
TFKGIYNMKIKACIFDMDGTLINSIMDIASSMNLALKKLGYREIKTEEFNTLVGRGYSKLIENTLKHLNIDLNDKHLNDN
LYQEFVKAYNQNLYFHTKTYDGIPELLKKLNSLKIPIGILSNKNHEELLIITKDIFKDIKFFEVRGYSSKFEAKPDPENA
LDMIIELNLMPQEIAYIGDSDVDMLTAQNAGFLPIGVSWGFRTIQELKENGAKYILNNPFELLDILK

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=210, Percent_Identity=24.7619047619048, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 26212; Mature: 26081

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFKGIYNMKIKACIFDMDGTLINSIMDIASSMNLALKKLGYREIKTEEFNTLVGRGYSK
CCCCCEECCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHH
LIENTLKHLNIDLNDKHLNDNLYQEFVKAYNQNLYFHTKTYDGIPELLKKLNSLKIPIGI
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCCEEEE
LSNKNHEELLIITKDIFKDIKFFEVRGYSSKFEAKPDPENALDMIIELNLMPQEIAYIGD
ECCCCCCEEEEEEHHHHHCHHEEEEECCCCCCCCCCCCHHHHEEHHEECCCHHHHHHCCC
SDVDMLTAQNAGFLPIGVSWGFRTIQELKENGAKYILNNPFELLDILK
CCCEEEEECCCCCEEEECCHHHHHHHHHHHCCCEEEECCCHHHHHHCC
>Mature Secondary Structure 
TFKGIYNMKIKACIFDMDGTLINSIMDIASSMNLALKKLGYREIKTEEFNTLVGRGYSK
CCCCEECCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHH
LIENTLKHLNIDLNDKHLNDNLYQEFVKAYNQNLYFHTKTYDGIPELLKKLNSLKIPIGI
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCCEEEE
LSNKNHEELLIITKDIFKDIKFFEVRGYSSKFEAKPDPENALDMIIELNLMPQEIAYIGD
ECCCCCCEEEEEEHHHHHCHHEEEEECCCCCCCCCCCCHHHHEEHHEECCCHHHHHHCCC
SDVDMLTAQNAGFLPIGVSWGFRTIQELKENGAKYILNNPFELLDILK
CCCEEEEECCCCCEEEECCHHHHHHHHHHHCCCEEEECCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782 [H]