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The map label for this gene is grpE [H]

Identifier: 203284428

GI number: 203284428

Start: 544748

End: 545296

Strand: Reverse

Name: grpE [H]

Synonym: BDU_521

Alternate gene names: 203284428

Gene position: 545296-544748 (Counterclockwise)

Preceding gene: 203284431

Following gene: 203284427

Centisome position: 58.53

GC content: 26.05

Gene sequence:

>549_bases
GTGGACGAAAAGGAAAAATGTGAAGACCCCGAAAAAATTAAAGAAAATGAAAATGAAACATTCCAAAGTGAAGATAACCC
TAACATGGAGAAAAAGATTATTGAACTTGAAAATGAAATTTCCAATCTAAAAGATTTATATTTAAGAAAACAAGCAGAAT
TTGAAAATTTTAGAAAAAGACTTGAAAAAGATAAAGAAAACTTTATTAAATTTGCAAATGAAAACATAATGAAAGATATA
ATTACTTTCCTTGACAATTTAGAGAGAGCAATAGATTCATCAAAGCAGTCAAAAGATTTTGATACCCTATTATCAGGAAT
TAGTATGATTGAAAATGAAATACTCTCAAGCTTTGACAAAAAATATAATCTAAAAAAATTTGGAAAACTAGGAGATGAAT
TTGATCCAAGTCAACATGAAGCAATAAGTATAGAAGAAAAAGAACAAATTATAAATCCTCAAATTGTAGAAGTATATCAA
AAAGGATACTGCTACAATAATCGAGTATTAAGAACTGCAAAAGTTAAAGTTGCACAAAGTAAAAATTAA

Upstream 100 bases:

>100_bases
AAGATAATTAAAATGAATCAAATTTAAAAAAATTTTCATAAAGAAAAAATTTGTAAAATATTTTTTTTATAGATAAAATG
CTTTATAAAAGGAGGCTAAA

Downstream 100 bases:

>100_bases
TTAGAATAATTAGAAATCAAAAAATAACAAAAGGAGGCCTTATATGGGAAAGATTATAGGAATTGATCTTGGAACAACAA
ATTCATGTGTAGCCATAATG

Product: chaperone protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MDEKEKCEDPEKIKENENETFQSEDNPNMEKKIIELENEISNLKDLYLRKQAEFENFRKRLEKDKENFIKFANENIMKDI
ITFLDNLERAIDSSKQSKDFDTLLSGISMIENEILSSFDKKYNLKKFGKLGDEFDPSQHEAISIEEKEQIINPQIVEVYQ
KGYCYNNRVLRTAKVKVAQSKN

Sequences:

>Translated_182_residues
MDEKEKCEDPEKIKENENETFQSEDNPNMEKKIIELENEISNLKDLYLRKQAEFENFRKRLEKDKENFIKFANENIMKDI
ITFLDNLERAIDSSKQSKDFDTLLSGISMIENEILSSFDKKYNLKKFGKLGDEFDPSQHEAISIEEKEQIINPQIVEVYQ
KGYCYNNRVLRTAKVKVAQSKN
>Mature_182_residues
MDEKEKCEDPEKIKENENETFQSEDNPNMEKKIIELENEISNLKDLYLRKQAEFENFRKRLEKDKENFIKFANENIMKDI
ITFLDNLERAIDSSKQSKDFDTLLSGISMIENEILSSFDKKYNLKKFGKLGDEFDPSQHEAISIEEKEQIINPQIVEVYQ
KGYCYNNRVLRTAKVKVAQSKN

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI40255109, Length=183, Percent_Identity=29.5081967213115, Blast_Score=75, Evalue=3e-14,
Organism=Homo sapiens, GI24308295, Length=181, Percent_Identity=29.8342541436464, Blast_Score=65, Evalue=4e-11,
Organism=Escherichia coli, GI1788967, Length=182, Percent_Identity=29.6703296703297, Blast_Score=88, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6324806, Length=158, Percent_Identity=31.0126582278481, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24653432, Length=177, Percent_Identity=30.5084745762712, Blast_Score=71, Evalue=4e-13,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 21516; Mature: 21516

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEKEKCEDPEKIKENENETFQSEDNPNMEKKIIELENEISNLKDLYLRKQAEFENFRKR
CCCCCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEKDKENFIKFANENIMKDIITFLDNLERAIDSSKQSKDFDTLLSGISMIENEILSSFDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
KYNLKKFGKLGDEFDPSQHEAISIEEKEQIINPQIVEVYQKGYCYNNRVLRTAKVKVAQS
HCCHHHHHCCCCCCCCCCCCEECHHHHHHHHCHHHHHHHHHCCCCCCCCHHHHHHHHHCC
KN
CC
>Mature Secondary Structure
MDEKEKCEDPEKIKENENETFQSEDNPNMEKKIIELENEISNLKDLYLRKQAEFENFRKR
CCCCCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEKDKENFIKFANENIMKDIITFLDNLERAIDSSKQSKDFDTLLSGISMIENEILSSFDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
KYNLKKFGKLGDEFDPSQHEAISIEEKEQIINPQIVEVYQKGYCYNNRVLRTAKVKVAQS
HCCHHHHHCCCCCCCCCCCCEECHHHHHHHHCHHHHHHHHHCCCCCCCCHHHHHHHHHCC
KN
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA