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The map label for this gene is gltX

Identifier: 203284285

GI number: 203284285

Start: 395144

End: 396664

Strand: Reverse

Name: gltX

Synonym: BDU_367

Alternate gene names: 203284285

Gene position: 396664-395144 (Counterclockwise)

Preceding gene: 203284286

Following gene: 203284284

Centisome position: 42.58

GC content: 30.51

Gene sequence:

>1521_bases
ATGATATTTCAAAAAAGGACATTTTTTATTAAAAGAGGTTGTGTTTTGAATATAAGAGTTAGATATGCTCCTTCTCCAAC
TGGTTTGCAGCATATTGGAGGAATTAGGACAGCTTTATTTAATTATTTTTTTGCCAAATCCTTTAATGGAAAATTTTTAC
TTAGAATTGAAGATACAGATCAGACTAGATATTATAAGGAAGCAGAAGAAGATTTGTATCAAAGTTTAGCATGGCTTGGT
ATTGATTTTGATGAAGGGCCTACTTGTGGAGGTTCTTATTCTCCTTATATTCAATCACAAAGAACAGAGATATATAGGAA
ATATGCTAAAGAGTTGATTGAATCGGGAAATGCTTATTATTGTTATTGTTCACCTGATAGATTAGAGAGAATTAGAAAAA
TTCAAACTATTAACAAGATGGCACCAGGTTATGATAGACATTGCAGGCACTTGAACAAAGATGAAATTAAAGATGCTTTA
AGTTTAGGCATTAGTCCTGTTATTAGATTTAAAATTCCTTTTGATGGTGAAACTTCTTTTAATGATATTTTGCTTGGCAA
AATTACATGGGCCAATAAGGATATTAGTCCTGATCCTGTAATTTTGAAATCTGATGGATTTCCAACATATCATCTTGCAA
ATGTTGTTGATGATCATTTAATGGAAATTTCACATGTATTAAGAGCCCAGGAGTGGATTTCTTCAGGACCTTTGCATGTG
CTTCTTTATAATGCTTTTAGATGGAATCCTCCTATTTATTGTCATCTTCCAATGGTAATGGGAAGTGATGGACAAAAATT
AAGTAAGAGGCATGGTGCTACAGCTTTAAAGCAATTTATTGATGATGGATATCTTCCTGAGGCTATTATTAATTATGTTA
CTTTGCTTGGTTGGTCTTATGATGGCAAGAGTGAGTTTTTTACCAAAAATGAACTTCAAAAATTATTTTCTATTGATAAA
ATCAGTAAATCACCAGCTGTTTTTGATTATAACAAATTAGATTTTTTTAATAGTCATTACATTAGGACAAAAGAAGATCA
TGAATTAGCCGAACTCTTATTGCCTTTCTTGCAAAAGGCAGGTTATATTAAAAAGGATAGTAATTCTTGTGACAAAGAAA
AATTATTGCTTTTGGTCCCGTTAATAAAGCCAAGAATTAGAAAACTTGGTGATGCTGTTGGCATGCTTAGGTTTTTTTAT
ACAAATATTTCAACTTGGAATTTAAATGAATTTTTGGGGAAGAAAAAAACAGTAAGAGATATTTATTTATTGTTAGAAAA
AATTAAACCAGTATTAGAAGGTTTTGAAACAAGGATATTGTCCGAGAATGAACAAATTTTTTATAATTTTGCTAAGGAAA
ATGATCTAAAAATAGGAGAGGTTTTGCTTCCAATTAGGATTGCAGTTCTTGGAAGTAAGGTATCACCACCTCTTTTTGAT
TCTTTGCAATTACTTGGTAAAGTTACGGTCTTTGATAGAATAAATAAAGCACAGGATTTTTTAAAAAAGTATGAATTATA
G

Upstream 100 bases:

>100_bases
GAAATTAGGAATAAGTATTTAAAACGTAAAGATAGACTTAATATTAAACATACAAAATTACTTGGTTCAAATTTATTTTT
TGTAGTGTTTTATTATATTT

Downstream 100 bases:

>100_bases
ATTAAGTTTTTAGGATGTTTGATATGGCTAAAATAGAAGATATTATTTCACTTGCTAAGAGGCGAGGTTTTGTATTTCAA
TCCTCAGAAGTTTATGGGGG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MIFQKRTFFIKRGCVLNIRVRYAPSPTGLQHIGGIRTALFNYFFAKSFNGKFLLRIEDTDQTRYYKEAEEDLYQSLAWLG
IDFDEGPTCGGSYSPYIQSQRTEIYRKYAKELIESGNAYYCYCSPDRLERIRKIQTINKMAPGYDRHCRHLNKDEIKDAL
SLGISPVIRFKIPFDGETSFNDILLGKITWANKDISPDPVILKSDGFPTYHLANVVDDHLMEISHVLRAQEWISSGPLHV
LLYNAFRWNPPIYCHLPMVMGSDGQKLSKRHGATALKQFIDDGYLPEAIINYVTLLGWSYDGKSEFFTKNELQKLFSIDK
ISKSPAVFDYNKLDFFNSHYIRTKEDHELAELLLPFLQKAGYIKKDSNSCDKEKLLLLVPLIKPRIRKLGDAVGMLRFFY
TNISTWNLNEFLGKKKTVRDIYLLLEKIKPVLEGFETRILSENEQIFYNFAKENDLKIGEVLLPIRIAVLGSKVSPPLFD
SLQLLGKVTVFDRINKAQDFLKKYEL

Sequences:

>Translated_506_residues
MIFQKRTFFIKRGCVLNIRVRYAPSPTGLQHIGGIRTALFNYFFAKSFNGKFLLRIEDTDQTRYYKEAEEDLYQSLAWLG
IDFDEGPTCGGSYSPYIQSQRTEIYRKYAKELIESGNAYYCYCSPDRLERIRKIQTINKMAPGYDRHCRHLNKDEIKDAL
SLGISPVIRFKIPFDGETSFNDILLGKITWANKDISPDPVILKSDGFPTYHLANVVDDHLMEISHVLRAQEWISSGPLHV
LLYNAFRWNPPIYCHLPMVMGSDGQKLSKRHGATALKQFIDDGYLPEAIINYVTLLGWSYDGKSEFFTKNELQKLFSIDK
ISKSPAVFDYNKLDFFNSHYIRTKEDHELAELLLPFLQKAGYIKKDSNSCDKEKLLLLVPLIKPRIRKLGDAVGMLRFFY
TNISTWNLNEFLGKKKTVRDIYLLLEKIKPVLEGFETRILSENEQIFYNFAKENDLKIGEVLLPIRIAVLGSKVSPPLFD
SLQLLGKVTVFDRINKAQDFLKKYEL
>Mature_506_residues
MIFQKRTFFIKRGCVLNIRVRYAPSPTGLQHIGGIRTALFNYFFAKSFNGKFLLRIEDTDQTRYYKEAEEDLYQSLAWLG
IDFDEGPTCGGSYSPYIQSQRTEIYRKYAKELIESGNAYYCYCSPDRLERIRKIQTINKMAPGYDRHCRHLNKDEIKDAL
SLGISPVIRFKIPFDGETSFNDILLGKITWANKDISPDPVILKSDGFPTYHLANVVDDHLMEISHVLRAQEWISSGPLHV
LLYNAFRWNPPIYCHLPMVMGSDGQKLSKRHGATALKQFIDDGYLPEAIINYVTLLGWSYDGKSEFFTKNELQKLFSIDK
ISKSPAVFDYNKLDFFNSHYIRTKEDHELAELLLPFLQKAGYIKKDSNSCDKEKLLLLVPLIKPRIRKLGDAVGMLRFFY
TNISTWNLNEFLGKKKTVRDIYLLLEKIKPVLEGFETRILSENEQIFYNFAKENDLKIGEVLLPIRIAVLGSKVSPPLFD
SLQLLGKVTVFDRINKAQDFLKKYEL

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=487, Percent_Identity=36.3449691991786, Blast_Score=303, Evalue=2e-82,
Organism=Homo sapiens, GI62241042, Length=218, Percent_Identity=27.0642201834862, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1788743, Length=491, Percent_Identity=35.8452138492872, Blast_Score=323, Evalue=1e-89,
Organism=Escherichia coli, GI226510925, Length=256, Percent_Identity=34.765625, Blast_Score=132, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI71993394, Length=505, Percent_Identity=35.8415841584158, Blast_Score=292, Evalue=2e-79,
Organism=Saccharomyces cerevisiae, GI6324540, Length=510, Percent_Identity=35.4901960784314, Blast_Score=296, Evalue=4e-81,
Organism=Saccharomyces cerevisiae, GI6324742, Length=311, Percent_Identity=27.0096463022508, Blast_Score=74, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6321192, Length=245, Percent_Identity=23.6734693877551, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24665321, Length=495, Percent_Identity=37.7777777777778, Blast_Score=337, Evalue=1e-92,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_BORDL (B5RLR1)

Other databases:

- EMBL:   CP000976
- RefSeq:   YP_002222025.1
- EnsemblBacteria:   EBBORT00000001456
- GeneID:   6918451
- GenomeReviews:   CP000976_GR
- KEGG:   bdu:BDU_367
- GeneTree:   EBGT00050000007156
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- ProtClustDB:   PRK01406
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 58556; Mature: 58556

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 269-269

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFQKRTFFIKRGCVLNIRVRYAPSPTGLQHIGGIRTALFNYFFAKSFNGKFLLRIEDTD
CCCCCCCCEEECCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
QTRYYKEAEEDLYQSLAWLGIDFDEGPTCGGSYSPYIQSQRTEIYRKYAKELIESGNAYY
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEE
CYCSPDRLERIRKIQTINKMAPGYDRHCRHLNKDEIKDALSLGISPVIRFKIPFDGETSF
EEECHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHCCCCEEEEEEECCCCCCCC
NDILLGKITWANKDISPDPVILKSDGFPTYHLANVVDDHLMEISHVLRAQEWISSGPLHV
CCEEEEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LLYNAFRWNPPIYCHLPMVMGSDGQKLSKRHGATALKQFIDDGYLPEAIINYVTLLGWSY
EEEECCCCCCCEEEEECEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
DGKSEFFTKNELQKLFSIDKISKSPAVFDYNKLDFFNSHYIRTKEDHELAELLLPFLQKA
CCCCCCCCHHHHHHHHHHHHCCCCCCEEECCHHCCCCCCCCCCCCHHHHHHHHHHHHHHC
GYIKKDSNSCDKEKLLLLVPLIKPRIRKLGDAVGMLRFFYTNISTWNLNEFLGKKKTVRD
CCCCCCCCCCCCHHEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHH
IYLLLEKIKPVLEGFETRILSENEQIFYNFAKENDLKIGEVLLPIRIAVLGSKVSPPLFD
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHEECCCCCCCCHHH
SLQLLGKVTVFDRINKAQDFLKKYEL
HHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIFQKRTFFIKRGCVLNIRVRYAPSPTGLQHIGGIRTALFNYFFAKSFNGKFLLRIEDTD
CCCCCCCCEEECCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
QTRYYKEAEEDLYQSLAWLGIDFDEGPTCGGSYSPYIQSQRTEIYRKYAKELIESGNAYY
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEE
CYCSPDRLERIRKIQTINKMAPGYDRHCRHLNKDEIKDALSLGISPVIRFKIPFDGETSF
EEECHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHCCCCEEEEEEECCCCCCCC
NDILLGKITWANKDISPDPVILKSDGFPTYHLANVVDDHLMEISHVLRAQEWISSGPLHV
CCEEEEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LLYNAFRWNPPIYCHLPMVMGSDGQKLSKRHGATALKQFIDDGYLPEAIINYVTLLGWSY
EEEECCCCCCCEEEEECEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
DGKSEFFTKNELQKLFSIDKISKSPAVFDYNKLDFFNSHYIRTKEDHELAELLLPFLQKA
CCCCCCCCHHHHHHHHHHHHCCCCCCEEECCHHCCCCCCCCCCCCHHHHHHHHHHHHHHC
GYIKKDSNSCDKEKLLLLVPLIKPRIRKLGDAVGMLRFFYTNISTWNLNEFLGKKKTVRD
CCCCCCCCCCCCHHEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHH
IYLLLEKIKPVLEGFETRILSENEQIFYNFAKENDLKIGEVLLPIRIAVLGSKVSPPLFD
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHEECCCCCCCCHHH
SLQLLGKVTVFDRINKAQDFLKKYEL
HHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA