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The map label for this gene is tyrS

Identifier: 203284283

GI number: 203284283

Start: 392567

End: 393787

Strand: Reverse

Name: tyrS

Synonym: BDU_365

Alternate gene names: 203284283

Gene position: 393787-392567 (Counterclockwise)

Preceding gene: 203284284

Following gene: 203284282

Centisome position: 42.27

GC content: 29.89

Gene sequence:

>1221_bases
ATGAATTTATCCCTAAAGGTTTTAGATAAGAGAGGATTTTTAAAACAGTGTACTAATTTGGAAGAGTTAAGTACATTGAT
GGATAGAGAAAAAATAGTTTTTTATGTTGGAGTTGATGCTACTTCTGCTTCTTTGCATATTGGACATTTAATTCCTTTTA
TGGTAATGCTTCATCTTCAAAGACAGGGTCATATTCCAATTATTTTAATTGGTGGTGGAACTACAAAAATAGGAGATCCT
TCTGGTAAGGATTCAATGCGCAAAATTTTATTAAAAGAAGATATAGATGAGAATGTTAAAACAATAAGTTCTCAATTGCT
TAAAATAATAGATCTTAGTAATGGTGGGTATATTCTTAATAATGCCGAATGGCTTGATGGCATTAATTATATTGAATTTT
TAAGGGAAGTTGGTATTTATTTTTCTGTTAATCGTATGTTAAGTTTTGAAACTTATAAGAGAAGATTAAAAGATGGTCTT
TCATTTATTGAATTTAATTATCAACTTTTACAGTCATATGATTTTTATATGTTAAGTCGTATGAAAAATTGTAAACTTCA
AATTGGTGGTGATGATCAGTGGGGAAATATTGTCTCAGGTGTTGATTTGGTTAATAGAAAATCGGGAAACAAAGTGTTTG
GGCTTACATTGCCGCTTATTACAAGAAGTGATGGTAAGAAAATGGGTAAATCAGAAAAAGGAGCTGTTTATCTTGATTCA
GAGCTTTATAGTGTTTATGATTTTTATCAGTATTTTAGAAACATTCCAGATTTAGATGTTAAGAAATTTTTATATTTATT
TACTTTTTTAGAAGAAGAAGAGATTGAACGTATTGCAAGTGTTAAGGGACAATTGTTAAATAATGCAAAAGAGATTTTGG
CATTTGAGATAACAAAAATCGTTCATGGTAAAGATGAGGCTTTAAAAGCATCCTCAGCAGCTAAAGCAGCATTTAAAGGA
GGTGACGGGAGAGCTGATATTCCTTTTTTTAAATTGGAATTGACTAATTTAGAGGAGAGTATTTTATTAGTTGACTTAAT
GGTTTTAGCAAAGGTTGTATCGAGTAAGTCAGAGGCTAGGCGACTTATTGATTCTGGTGGAGTTTATATAGATAAAGTAA
GGGTAGGAGATCAAAATTATTGCCTTTGTAAGGACAATTTTATCAATGGTGAAATTGAACTTAAAATAGGCAAGAAAAAG
ATTTTGAGAATTGTTTTATAA

Upstream 100 bases:

>100_bases
TTAAGAGATAGAGATACTATGACTCAAACAAGAATTTCTATTGATGATTTGTATTCATATGTTAAAACGGGAATTCTAAA
TTACAAGGGAGTTTAAATAA

Downstream 100 bases:

>100_bases
TTGGTGTTTCATGTATAACAGAAGAGCTTTAAATCATTTGTTTTTTAAATCGTTTCTGTTTTTTGTGGAACGTAAACATC
TTTTTTTTACCGAAGAACAT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MNLSLKVLDKRGFLKQCTNLEELSTLMDREKIVFYVGVDATSASLHIGHLIPFMVMLHLQRQGHIPIILIGGGTTKIGDP
SGKDSMRKILLKEDIDENVKTISSQLLKIIDLSNGGYILNNAEWLDGINYIEFLREVGIYFSVNRMLSFETYKRRLKDGL
SFIEFNYQLLQSYDFYMLSRMKNCKLQIGGDDQWGNIVSGVDLVNRKSGNKVFGLTLPLITRSDGKKMGKSEKGAVYLDS
ELYSVYDFYQYFRNIPDLDVKKFLYLFTFLEEEEIERIASVKGQLLNNAKEILAFEITKIVHGKDEALKASSAAKAAFKG
GDGRADIPFFKLELTNLEESILLVDLMVLAKVVSSKSEARRLIDSGGVYIDKVRVGDQNYCLCKDNFINGEIELKIGKKK
ILRIVL

Sequences:

>Translated_406_residues
MNLSLKVLDKRGFLKQCTNLEELSTLMDREKIVFYVGVDATSASLHIGHLIPFMVMLHLQRQGHIPIILIGGGTTKIGDP
SGKDSMRKILLKEDIDENVKTISSQLLKIIDLSNGGYILNNAEWLDGINYIEFLREVGIYFSVNRMLSFETYKRRLKDGL
SFIEFNYQLLQSYDFYMLSRMKNCKLQIGGDDQWGNIVSGVDLVNRKSGNKVFGLTLPLITRSDGKKMGKSEKGAVYLDS
ELYSVYDFYQYFRNIPDLDVKKFLYLFTFLEEEEIERIASVKGQLLNNAKEILAFEITKIVHGKDEALKASSAAKAAFKG
GDGRADIPFFKLELTNLEESILLVDLMVLAKVVSSKSEARRLIDSGGVYIDKVRVGDQNYCLCKDNFINGEIELKIGKKK
ILRIVL
>Mature_406_residues
MNLSLKVLDKRGFLKQCTNLEELSTLMDREKIVFYVGVDATSASLHIGHLIPFMVMLHLQRQGHIPIILIGGGTTKIGDP
SGKDSMRKILLKEDIDENVKTISSQLLKIIDLSNGGYILNNAEWLDGINYIEFLREVGIYFSVNRMLSFETYKRRLKDGL
SFIEFNYQLLQSYDFYMLSRMKNCKLQIGGDDQWGNIVSGVDLVNRKSGNKVFGLTLPLITRSDGKKMGKSEKGAVYLDS
ELYSVYDFYQYFRNIPDLDVKKFLYLFTFLEEEEIERIASVKGQLLNNAKEILAFEITKIVHGKDEALKASSAAKAAFKG
GDGRADIPFFKLELTNLEESILLVDLMVLAKVVSSKSEARRLIDSGGVYIDKVRVGDQNYCLCKDNFINGEIELKIGKKK
ILRIVL

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=420, Percent_Identity=34.5238095238095, Blast_Score=214, Evalue=2e-55,
Organism=Escherichia coli, GI1787925, Length=416, Percent_Identity=37.5, Blast_Score=279, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI25145802, Length=304, Percent_Identity=39.4736842105263, Blast_Score=188, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI25145805, Length=242, Percent_Identity=37.603305785124, Blast_Score=139, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6325160, Length=437, Percent_Identity=35.9267734553776, Blast_Score=246, Evalue=5e-66,
Organism=Drosophila melanogaster, GI20130383, Length=426, Percent_Identity=34.9765258215962, Blast_Score=244, Evalue=5e-65,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_BORDL (B5RLQ9)

Other databases:

- EMBL:   CP000976
- RefSeq:   YP_002222023.1
- EnsemblBacteria:   EBBORT00000001526
- GeneID:   6918449
- GenomeReviews:   CP000976_GR
- KEGG:   bdu:BDU_365
- GeneTree:   EBGT00050000006942
- HOGENOM:   HBG288125
- OMA:   WINQDDR
- ProtClustDB:   PRK05912
- GO:   GO:0005737
- HAMAP:   MF_02006
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 45997; Mature: 45997

Theoretical pI: Translated: 8.51; Mature: 8.51

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 35-35 BINDING 167-167 BINDING 171-171 BINDING 230-230

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLSLKVLDKRGFLKQCTNLEELSTLMDREKIVFYVGVDATSASLHIGHLIPFMVMLHLQ
CCCEEEEECCCCHHHHHCCHHHHHHHHCCCEEEEEEECCCCCCCEEHHHHHHHHHHHHHH
RQGHIPIILIGGGTTKIGDPSGKDSMRKILLKEDIDENVKTISSQLLKIIDLSNGGYILN
CCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEC
NAEWLDGINYIEFLREVGIYFSVNRMLSFETYKRRLKDGLSFIEFNYQLLQSYDFYMLSR
CCHHHCCCHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCHHHHHH
MKNCKLQIGGDDQWGNIVSGVDLVNRKSGNKVFGLTLPLITRSDGKKMGKSEKGAVYLDS
CCCCEEEECCCCCCCHHHHHHHHHCCCCCCEEEEEEEEEEECCCCHHCCCCCCCEEEECH
ELYSVYDFYQYFRNIPDLDVKKFLYLFTFLEEEEIERIASVKGQLLNNAKEILAFEITKI
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VHGKDEALKASSAAKAAFKGGDGRADIPFFKLELTNLEESILLVDLMVLAKVVSSKSEAR
HCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCHHHHH
RLIDSGGVYIDKVRVGDQNYCLCKDNFINGEIELKIGKKKILRIVL
HHHHCCCEEEEEEEECCCCEEEEECCCCCCEEEEEECHHHHHEECC
>Mature Secondary Structure
MNLSLKVLDKRGFLKQCTNLEELSTLMDREKIVFYVGVDATSASLHIGHLIPFMVMLHLQ
CCCEEEEECCCCHHHHHCCHHHHHHHHCCCEEEEEEECCCCCCCEEHHHHHHHHHHHHHH
RQGHIPIILIGGGTTKIGDPSGKDSMRKILLKEDIDENVKTISSQLLKIIDLSNGGYILN
CCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEC
NAEWLDGINYIEFLREVGIYFSVNRMLSFETYKRRLKDGLSFIEFNYQLLQSYDFYMLSR
CCHHHCCCHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCHHHHHH
MKNCKLQIGGDDQWGNIVSGVDLVNRKSGNKVFGLTLPLITRSDGKKMGKSEKGAVYLDS
CCCCEEEECCCCCCCHHHHHHHHHCCCCCCEEEEEEEEEEECCCCHHCCCCCCCEEEECH
ELYSVYDFYQYFRNIPDLDVKKFLYLFTFLEEEEIERIASVKGQLLNNAKEILAFEITKI
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VHGKDEALKASSAAKAAFKGGDGRADIPFFKLELTNLEESILLVDLMVLAKVVSSKSEAR
HCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCHHHHH
RLIDSGGVYIDKVRVGDQNYCLCKDNFINGEIELKIGKKKILRIVL
HHHHCCCEEEEEEEECCCCEEEEECCCCCCEEEEEECHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA