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The map label for this gene is uvrD [H]

Identifier: 203284261

GI number: 203284261

Start: 365704

End: 367800

Strand: Reverse

Name: uvrD [H]

Synonym: BDU_343

Alternate gene names: 203284261

Gene position: 367800-365704 (Counterclockwise)

Preceding gene: 203284262

Following gene: 203284260

Centisome position: 39.48

GC content: 26.94

Gene sequence:

>2097_bases
TTGATGAGTGAAATACAAAAATTTCTTTTTAGTTTAAATTTTGATCAAAAGCAAATTGTCTTTGATGATACTACGAACCC
CATTCTTGTTTTGGCAGGGCCAGGCAGTGGTAAGACGAGGGTTATAACAGCTAAGTTTGCGCATTTAATAAATGAAGGTA
AAGTAAAACCAGAAGAAATTCTTGCTTTAACGTTTACAAATAAAGCAGCACGTGAAATGAATTTTAGATTGAATTCTCTT
TTTAATTTTGATAGGTCTTTACATATTCAAACTTTTCATTCTTTTGGTGCTTGGCTTTTGCGACTTTACTTTAAAGAATA
TGATGAAAATTATGATTCAAATTTTACAATTTGGGATGTTAATGATGTTGTTAAATTTGTTAAACAAATTGGACTTGCAT
CAAATATTGAATGTGCCAAGTATGTAACATCGTCAATATTGAAATATAAGGAGAATTATTCTTTACATAATAATTGTGGT
TTTGATGATAAAATTTATTCTGAGATTGAATTTTATGAACAGGAAAAATCTAAAAGTAATGCTTTTGATTTTGCCGATCT
TATTCTTAAATCTGTTTTGATGTTACAAAATTGCGAAAATATTAGAATGAGGGTGCAAAAAAGATTTAAGGCTATTTTTG
TGGATGAATATCAAGATACTAATTATTCACAGTTTTTGTTTTTAAGAGAACTGTATTATAAAGATGCATATTTTATGGTA
GTGGGAGATGAGGATCAGTCTATATATTCTTTTAGAGGTGCTAGAGTTGAAAATATTCTTGAATTTGAAAGAACATTTGA
TAATGTGTCTAAATATTATTTGGTCCAAAATTATCGTTCTAGTTTAAGTATTGTCAATGTTGCAAATAATATTATTTCAA
AAAATCAAAATAGGTATGATAAAGTAATAGCTACGGAAAATAAGATAGGTAAAAAAATAAAAATTTTTATATTTCAAAAT
CCTACAGAAGAAGCTGAGTATTTTGCAAATTTTCTTATTGAAAATAAGCTTGAAACAGCTGTTCTTTATAGATTTAATCA
TCAGTCTTTGCAATTTGAGAAGGCTTTTTTTAAAAATAATATTGCGCATAAAATATTAGGTTCAATTAGATTCTATGAAA
GAGAAGAAATTAAGGATGTCATATCTTTGTTAAGACTTTTTGTAAATAAAAAAGATAAAGTATCTTTTTTGAGAATAATA
AATAAGCCTGCAAGAGGTCTTGGTAAAACTACCATTGATAAGATAATTGCGACATTAAATGATACTGATGTGAATCTTGA
CTTAATGCTTGCAAGTAGAAAAGTTGTTAAAAATCTTAAAGGAAAAGCAAGAGATTCTCTTGATATATTTTTAAGTTTAT
ATGATGAATTAAAAGAAAATATACAAAAGGGTATATATGTAAATTTATCTGAATTTATTAAAGATGTTACAATGGGATTT
GGAATTTGGAATTATTATCAAAAATTTGATAAGGATGAGAAATCAAAAAATATTGATGAGCTTATTAGTAGTGGAGTTGA
ATATTCTGGTAGTTTTGAAGGTCTTGTGATATTTCTTGAAAATTCGTCTTTGTCTCCTTTAGTTCATGGAGATTTTGCAT
CTAGTGTGTTACTCTCTTCAATTCATGGCGTTAAAGGACTTGAGTTTGATAGGGTAATAATATCTGGTCTTGAGAAAGGT
TTATTACCTGCTGAGATTGAAGAATTGACAGCTGATAGATTAGAAGAAGAGAGGAGACTTTTTTATGTTGCTATTACTAG
GGCTAAATTCGAACTTTTTATTACAATAAATTTGCAAAGATTTTTTAGAGGAATATTAAAAAATACAGCTATATCAGTTT
TTTTTCAAGAGATCCATAAGGATAATTATGATATTGTTTTTGTTCCAGAATATTTGAAAGATAATTTTAAATTTTTTTTT
ACACGAAGTGATAATAAAAATTTTAATATTGGCGATTATATAATTTATAATGGTGAGAATGGCATTATTGTTGATAAGTG
GTACCAAGATGGTGGACCATTTCTTAAGATTAGTTTGAAAAATGGGAAAAAAGCTATTTTGAGTTCAAGTTATATTAAAA
AACTTTCTAAAATGTAG

Upstream 100 bases:

>100_bases
TTACTTCTGTTTTAAAAAGTTATGGAAGTGAAATGATAGCAATTCAATTTTTTTGGTTAAAAACACCAGGTGGTTTTGAT
CTTAAAGTTTCTGAAGTTTA

Downstream 100 bases:

>100_bases
AGGTGAAATTTGAAAAATATTCATTTAGAGAATAGTTTAAAATTGAGTTTATTAAAGTTAAGTGAAGATGAAAAGCAGCA
ATTTGTTATGAAATTTGAAA

Product: DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 698; Mature: 698

Protein sequence:

>698_residues
MMSEIQKFLFSLNFDQKQIVFDDTTNPILVLAGPGSGKTRVITAKFAHLINEGKVKPEEILALTFTNKAAREMNFRLNSL
FNFDRSLHIQTFHSFGAWLLRLYFKEYDENYDSNFTIWDVNDVVKFVKQIGLASNIECAKYVTSSILKYKENYSLHNNCG
FDDKIYSEIEFYEQEKSKSNAFDFADLILKSVLMLQNCENIRMRVQKRFKAIFVDEYQDTNYSQFLFLRELYYKDAYFMV
VGDEDQSIYSFRGARVENILEFERTFDNVSKYYLVQNYRSSLSIVNVANNIISKNQNRYDKVIATENKIGKKIKIFIFQN
PTEEAEYFANFLIENKLETAVLYRFNHQSLQFEKAFFKNNIAHKILGSIRFYEREEIKDVISLLRLFVNKKDKVSFLRII
NKPARGLGKTTIDKIIATLNDTDVNLDLMLASRKVVKNLKGKARDSLDIFLSLYDELKENIQKGIYVNLSEFIKDVTMGF
GIWNYYQKFDKDEKSKNIDELISSGVEYSGSFEGLVIFLENSSLSPLVHGDFASSVLLSSIHGVKGLEFDRVIISGLEKG
LLPAEIEELTADRLEEERRLFYVAITRAKFELFITINLQRFFRGILKNTAISVFFQEIHKDNYDIVFVPEYLKDNFKFFF
TRSDNKNFNIGDYIIYNGENGIIVDKWYQDGGPFLKISLKNGKKAILSSSYIKKLSKM

Sequences:

>Translated_698_residues
MMSEIQKFLFSLNFDQKQIVFDDTTNPILVLAGPGSGKTRVITAKFAHLINEGKVKPEEILALTFTNKAAREMNFRLNSL
FNFDRSLHIQTFHSFGAWLLRLYFKEYDENYDSNFTIWDVNDVVKFVKQIGLASNIECAKYVTSSILKYKENYSLHNNCG
FDDKIYSEIEFYEQEKSKSNAFDFADLILKSVLMLQNCENIRMRVQKRFKAIFVDEYQDTNYSQFLFLRELYYKDAYFMV
VGDEDQSIYSFRGARVENILEFERTFDNVSKYYLVQNYRSSLSIVNVANNIISKNQNRYDKVIATENKIGKKIKIFIFQN
PTEEAEYFANFLIENKLETAVLYRFNHQSLQFEKAFFKNNIAHKILGSIRFYEREEIKDVISLLRLFVNKKDKVSFLRII
NKPARGLGKTTIDKIIATLNDTDVNLDLMLASRKVVKNLKGKARDSLDIFLSLYDELKENIQKGIYVNLSEFIKDVTMGF
GIWNYYQKFDKDEKSKNIDELISSGVEYSGSFEGLVIFLENSSLSPLVHGDFASSVLLSSIHGVKGLEFDRVIISGLEKG
LLPAEIEELTADRLEEERRLFYVAITRAKFELFITINLQRFFRGILKNTAISVFFQEIHKDNYDIVFVPEYLKDNFKFFF
TRSDNKNFNIGDYIIYNGENGIIVDKWYQDGGPFLKISLKNGKKAILSSSYIKKLSKM
>Mature_698_residues
MMSEIQKFLFSLNFDQKQIVFDDTTNPILVLAGPGSGKTRVITAKFAHLINEGKVKPEEILALTFTNKAAREMNFRLNSL
FNFDRSLHIQTFHSFGAWLLRLYFKEYDENYDSNFTIWDVNDVVKFVKQIGLASNIECAKYVTSSILKYKENYSLHNNCG
FDDKIYSEIEFYEQEKSKSNAFDFADLILKSVLMLQNCENIRMRVQKRFKAIFVDEYQDTNYSQFLFLRELYYKDAYFMV
VGDEDQSIYSFRGARVENILEFERTFDNVSKYYLVQNYRSSLSIVNVANNIISKNQNRYDKVIATENKIGKKIKIFIFQN
PTEEAEYFANFLIENKLETAVLYRFNHQSLQFEKAFFKNNIAHKILGSIRFYEREEIKDVISLLRLFVNKKDKVSFLRII
NKPARGLGKTTIDKIIATLNDTDVNLDLMLASRKVVKNLKGKARDSLDIFLSLYDELKENIQKGIYVNLSEFIKDVTMGF
GIWNYYQKFDKDEKSKNIDELISSGVEYSGSFEGLVIFLENSSLSPLVHGDFASSVLLSSIHGVKGLEFDRVIISGLEKG
LLPAEIEELTADRLEEERRLFYVAITRAKFELFITINLQRFFRGILKNTAISVFFQEIHKDNYDIVFVPEYLKDNFKFFF
TRSDNKNFNIGDYIIYNGENGIIVDKWYQDGGPFLKISLKNGKKAILSSSYIKKLSKM

Specific function: Has both ATPase and helicase activities. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Involved in the post-incision events of nucleotide exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=625, Percent_Identity=33.76, Blast_Score=325, Evalue=6e-90,
Organism=Escherichia coli, GI48994965, Length=657, Percent_Identity=33.9421613394216, Blast_Score=293, Evalue=3e-80,
Organism=Escherichia coli, GI1789183, Length=165, Percent_Identity=26.6666666666667, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322369, Length=732, Percent_Identity=28.0054644808743, Blast_Score=187, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6324477, Length=284, Percent_Identity=26.056338028169, Blast_Score=78, Evalue=4e-15,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005753
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 81345; Mature: 81345

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSEIQKFLFSLNFDQKQIVFDDTTNPILVLAGPGSGKTRVITAKFAHLINEGKVKPEEI
CHHHHHHHHHHCCCCCCEEEEECCCCCEEEEECCCCCCEEEEHHHHHHHHHCCCCCHHHE
LALTFTNKAAREMNFRLNSLFNFDRSLHIQTFHSFGAWLLRLYFKEYDENYDSNFTIWDV
EEEEECCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEH
NDVVKFVKQIGLASNIECAKYVTSSILKYKENYSLHNNCGFDDKIYSEIEFYEQEKSKSN
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
AFDFADLILKSVLMLQNCENIRMRVQKRFKAIFVDEYQDTNYSQFLFLRELYYKDAYFMV
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHCCEEEEE
VGDEDQSIYSFRGARVENILEFERTFDNVSKYYLVQNYRSSLSIVNVANNIISKNQNRYD
ECCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
KVIATENKIGKKIKIFIFQNPTEEAEYFANFLIENKLETAVLYRFNHQSLQFEKAFFKNN
HHEECCCCCCCEEEEEEEECCCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHCC
IAHKILGSIRFYEREEIKDVISLLRLFVNKKDKVSFLRIINKPARGLGKTTIDKIIATLN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCC
DTDVNLDLMLASRKVVKNLKGKARDSLDIFLSLYDELKENIQKGIYVNLSEFIKDVTMGF
CCCCEEEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHH
GIWNYYQKFDKDEKSKNIDELISSGVEYSGSFEGLVIFLENSSLSPLVHGDFASSVLLSS
HHHHHHHHHCCCHHCCCHHHHHHCCCCCCCCCCEEEEEEECCCCCCEECCHHHHHHHHHH
IHGVKGLEFDRVIISGLEKGLLPAEIEELTADRLEEERRLFYVAITRAKFELFITINLQR
HCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCEEEEEEEEEEEEEEEEEEEHHH
FFRGILKNTAISVFFQEIHKDNYDIVFVPEYLKDNFKFFFTRSDNKNFNIGDYIIYNGEN
HHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHCCCEEEEEEECCCCCCCCCCEEEEECCC
GIIVDKWYQDGGPFLKISLKNGKKAILSSSYIKKLSKM
CEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMSEIQKFLFSLNFDQKQIVFDDTTNPILVLAGPGSGKTRVITAKFAHLINEGKVKPEEI
CHHHHHHHHHHCCCCCCEEEEECCCCCEEEEECCCCCCEEEEHHHHHHHHHCCCCCHHHE
LALTFTNKAAREMNFRLNSLFNFDRSLHIQTFHSFGAWLLRLYFKEYDENYDSNFTIWDV
EEEEECCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEH
NDVVKFVKQIGLASNIECAKYVTSSILKYKENYSLHNNCGFDDKIYSEIEFYEQEKSKSN
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
AFDFADLILKSVLMLQNCENIRMRVQKRFKAIFVDEYQDTNYSQFLFLRELYYKDAYFMV
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHCCEEEEE
VGDEDQSIYSFRGARVENILEFERTFDNVSKYYLVQNYRSSLSIVNVANNIISKNQNRYD
ECCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
KVIATENKIGKKIKIFIFQNPTEEAEYFANFLIENKLETAVLYRFNHQSLQFEKAFFKNN
HHEECCCCCCCEEEEEEEECCCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHCC
IAHKILGSIRFYEREEIKDVISLLRLFVNKKDKVSFLRIINKPARGLGKTTIDKIIATLN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCC
DTDVNLDLMLASRKVVKNLKGKARDSLDIFLSLYDELKENIQKGIYVNLSEFIKDVTMGF
CCCCEEEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHH
GIWNYYQKFDKDEKSKNIDELISSGVEYSGSFEGLVIFLENSSLSPLVHGDFASSVLLSS
HHHHHHHHHCCCHHCCCHHHHHHCCCCCCCCCCEEEEEEECCCCCCEECCHHHHHHHHHH
IHGVKGLEFDRVIISGLEKGLLPAEIEELTADRLEEERRLFYVAITRAKFELFITINLQR
HCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCEEEEEEEEEEEEEEEEEEEHHH
FFRGILKNTAISVFFQEIHKDNYDIVFVPEYLKDNFKFFFTRSDNKNFNIGDYIIYNGEN
HHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHCCCEEEEEEECCCCCCCCCCEEEEECCC
GIIVDKWYQDGGPFLKISLKNGKKAILSSSYIKKLSKM
CEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6379604; 2943729; 1379743; 9278503; 6324092; 2254268; 8419285 [H]