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The map label for this gene is ftsJ

Identifier: 203284234

GI number: 203284234

Start: 335223

End: 335804

Strand: Reverse

Name: ftsJ

Synonym: BDU_316

Alternate gene names: 203284234

Gene position: 335804-335223 (Counterclockwise)

Preceding gene: 203284237

Following gene: 203284233

Centisome position: 36.04

GC content: 27.84

Gene sequence:

>582_bases
ATGTATAATGTTTTTGATGAATATTCACAAAAAGCTAAAAATGAGGGGTATCTTGCTAGATCTGTTTATAAATTGATTGA
AATTGATAAAAAATTTTCTTTGTTCTCTTCTGGTAATATATTAGATATTGGAGCATCTCCTGGCAGTTTTTCTCAATATG
CTTATGCTAATCTTAAGAATGGAGTGCTTGTTGCAGTTGACCTTAATGATGTAAATCTTAATTTTACTAGTAATTTTTAT
TTTATTAAGGGCAATATATATCTTGATGAGGTTTATCAGAAAATCAAAATTTTTTCACCTTATAGTTTGATTGTAAGCGA
TGCAGCTCCTAGTACTACTGGTAATAGATTAGTTGATACTAGTAATTCTTTTAATCTAAATATCAGAATATTTGAGTTAG
CTTGTGAGAGTTTGATGAGAGGTGGAAATTTGTTAATTAAGGTTTTTCAAGGTGGTGAGGAAGAACAGATTTTTTATAAA
CTTAAGAGTTGTTTTAGAGTTGTTAAAAAAGTGAGACCTAAAGCTGTTCGTAAAAATTCCTTTGAAATTTATTTTTTAGC
TAAAGATTTTGCTAAATTATAG

Upstream 100 bases:

>100_bases
ATTATTGATATTTTTTTCGATTTTATCTAAAAATGATTTATTTTGCATAATTATAATTATAATAAAAAGTATGATGATTA
AGTGTGTTTAAGAGAGTTAT

Downstream 100 bases:

>100_bases
AGTTATTTTTAAATTGTTTTTTGTTGAGGATTGTTAAAGTATGTTTATAAAGGATAAAATTATGGAAAAAAAATCTCATT
TACAAGTTGCATGTTTTAAA

Product: ribosomal RNA methyltransferase

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 193; Mature: 193

Protein sequence:

>193_residues
MYNVFDEYSQKAKNEGYLARSVYKLIEIDKKFSLFSSGNILDIGASPGSFSQYAYANLKNGVLVAVDLNDVNLNFTSNFY
FIKGNIYLDEVYQKIKIFSPYSLIVSDAAPSTTGNRLVDTSNSFNLNIRIFELACESLMRGGNLLIKVFQGGEEEQIFYK
LKSCFRVVKKVRPKAVRKNSFEIYFLAKDFAKL

Sequences:

>Translated_193_residues
MYNVFDEYSQKAKNEGYLARSVYKLIEIDKKFSLFSSGNILDIGASPGSFSQYAYANLKNGVLVAVDLNDVNLNFTSNFY
FIKGNIYLDEVYQKIKIFSPYSLIVSDAAPSTTGNRLVDTSNSFNLNIRIFELACESLMRGGNLLIKVFQGGEEEQIFYK
LKSCFRVVKKVRPKAVRKNSFEIYFLAKDFAKL
>Mature_193_residues
MYNVFDEYSQKAKNEGYLARSVYKLIEIDKKFSLFSSGNILDIGASPGSFSQYAYANLKNGVLVAVDLNDVNLNFTSNFY
FIKGNIYLDEVYQKIKIFSPYSLIVSDAAPSTTGNRLVDTSNSFNLNIRIFELACESLMRGGNLLIKVFQGGEEEQIFYK
LKSCFRVVKKVRPKAVRKNSFEIYFLAKDFAKL

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI7019377, Length=201, Percent_Identity=27.8606965174129, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI194097365, Length=191, Percent_Identity=27.2251308900524, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI7110661, Length=192, Percent_Identity=30.7291666666667, Blast_Score=83, Evalue=1e-16,
Organism=Homo sapiens, GI29029591, Length=192, Percent_Identity=30.7291666666667, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI29029589, Length=192, Percent_Identity=30.7291666666667, Blast_Score=83, Evalue=2e-16,
Organism=Escherichia coli, GI1789569, Length=188, Percent_Identity=30.8510638297872, Blast_Score=93, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17553474, Length=195, Percent_Identity=32.8205128205128, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17554650, Length=194, Percent_Identity=28.8659793814433, Blast_Score=78, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71987550, Length=193, Percent_Identity=26.4248704663212, Blast_Score=70, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17553860, Length=193, Percent_Identity=26.4248704663212, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI71987561, Length=193, Percent_Identity=26.4248704663212, Blast_Score=70, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6319796, Length=192, Percent_Identity=26.5625, Blast_Score=74, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6319535, Length=197, Percent_Identity=25.8883248730964, Blast_Score=72, Evalue=6e-14,
Organism=Drosophila melanogaster, GI21356387, Length=205, Percent_Identity=29.2682926829268, Blast_Score=88, Evalue=3e-18,
Organism=Drosophila melanogaster, GI18859957, Length=192, Percent_Identity=26.5625, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648639, Length=193, Percent_Identity=25.9067357512953, Blast_Score=70, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_BORDL (B5RLD9)

Other databases:

- EMBL:   CP000976
- RefSeq:   YP_002221974.1
- EnsemblBacteria:   EBBORT00000001440
- GeneID:   6918400
- GenomeReviews:   CP000976_GR
- KEGG:   bdu:BDU_316
- GeneTree:   EBGT00050000007024
- HOGENOM:   HBG398270
- OMA:   NYRCRSA
- ProtClustDB:   CLSK507509
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 21956; Mature: 21956

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: NA

Important sites: ACT_SITE 147-147 BINDING 48-48 BINDING 50-50 BINDING 67-67 BINDING 85-85 BINDING 107-107

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYNVFDEYSQKAKNEGYLARSVYKLIEIDKKFSLFSSGNILDIGASPGSFSQYAYANLKN
CCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEECCCCEEEECCCCCCCCCEEEEECCC
GVLVAVDLNDVNLNFTSNFYFIKGNIYLDEVYQKIKIFSPYSLIVSDAAPSTTGNRLVDT
CEEEEEEECCEEEEEECCEEEEECCEEHHHHHHHHEECCCCEEEEECCCCCCCCCEEEEC
SNSFNLNIRIFELACESLMRGGNLLIKVFQGGEEEQIFYKLKSCFRVVKKVRPKAVRKNS
CCCEEEEEEEHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHCCCC
FEIYFLAKDFAKL
EEEEEEECCHHCC
>Mature Secondary Structure
MYNVFDEYSQKAKNEGYLARSVYKLIEIDKKFSLFSSGNILDIGASPGSFSQYAYANLKN
CCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEECCCCEEEECCCCCCCCCEEEEECCC
GVLVAVDLNDVNLNFTSNFYFIKGNIYLDEVYQKIKIFSPYSLIVSDAAPSTTGNRLVDT
CEEEEEEECCEEEEEECCEEEEECCEEHHHHHHHHEECCCCEEEEECCCCCCCCCEEEEC
SNSFNLNIRIFELACESLMRGGNLLIKVFQGGEEEQIFYKLKSCFRVVKKVRPKAVRKNS
CCCEEEEEEEHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHCCCC
FEIYFLAKDFAKL
EEEEEEECCHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA