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The map label for this gene is rsmH

Identifier: 203284227

GI number: 203284227

Start: 328601

End: 329494

Strand: Reverse

Name: rsmH

Synonym: BDU_309

Alternate gene names: 203284227

Gene position: 329494-328601 (Counterclockwise)

Preceding gene: 203284228

Following gene: 203284226

Centisome position: 35.37

GC content: 28.08

Gene sequence:

>894_bases
ATGGGTGACATTTTTCATATTCCTGTGCTTCTTGAGGATGTTATTAATCTTGTAGAAACTATATATTTAGATGATGGATT
TGTTTTTGTTGATTGTACTCTTGGAGAAGGTGGTCATTCAAAAGCTGTACTTAGTAAATATCAAAATATTAATGTTATTG
GAATTGAAAGAGATGATGTTGTTTTGAATAGGGCAAAAGAATCTCTTGTAGAATTTAGTAAAAGAATTTCCTATTCTAAT
ACTTGGTTTGATGATTTTTTTTGTAAATATTCTTTACATCGTCGATTTAATTTTATTTTAGCTGATCTTGGTATTTCTAT
GTTTCATTACAAAGTTGGTGGTAGAGGATTTTCTTTTTTGGAAGATGAAAAATTAGATATGAGGCTTTTTCCTTCTGATG
GGGGTATTAGTGCTTATGATATTGTTAATACTTTTGATAAAGAAATGCTTGAAAATTTGATTTATGAATTGGGTGGTGAA
TATTATTCTAGGAGAATTGTTAAGGCTATCTTAGAGTATAGAAAAATTAATAAGATTCAAACCTCAAGAGAACTCCAGAG
AATAATTTGTAAAGCGTATCCTAATGTAAAATTTAAAATAAATCCAGCAACAAAAACTTTCCAAGCATTAAGAATTTATG
TTAATGATGAGTTGGATCGTTTAAAGAGAAGTTTGCCATTATGGATAGCAAATTTGTCAAAGAATGGAATTTTGGCCATT
ATTACATTTCATTCTTTGGAAGATAAAATTGTAAAAGAATTTTTTAAAGGTTTGACTAAAGATCAGTATTGCATACTTAC
TAAAAAACCTATAACCTCAAGTTTTAAAGAAAAACAGTACAATAATGCATCAAGAAGTGCTAAGCTAAGGGCTGTAAAAA
AATTATATGAGTAA

Upstream 100 bases:

>100_bases
ATAGATAATCTTGAATTTACTAATGGTAGTAATGAGATTAAAATCAAATATGGGGATGTTTATAATGTCGAAAATAGTTT
AACTTACAGGTTTACACTTA

Downstream 100 bases:

>100_bases
GATTAAAAGATTTGAAATTAGTTTTTACTGTTTATTAATTTTGCTATTAACAATTATAGTATGTTTAAATATTTATTTTA
ATTTTAGATATGTTGTAAAG

Product: S-adenosyl-methyltransferase

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MGDIFHIPVLLEDVINLVETIYLDDGFVFVDCTLGEGGHSKAVLSKYQNINVIGIERDDVVLNRAKESLVEFSKRISYSN
TWFDDFFCKYSLHRRFNFILADLGISMFHYKVGGRGFSFLEDEKLDMRLFPSDGGISAYDIVNTFDKEMLENLIYELGGE
YYSRRIVKAILEYRKINKIQTSRELQRIICKAYPNVKFKINPATKTFQALRIYVNDELDRLKRSLPLWIANLSKNGILAI
ITFHSLEDKIVKEFFKGLTKDQYCILTKKPITSSFKEKQYNNASRSAKLRAVKKLYE

Sequences:

>Translated_297_residues
MGDIFHIPVLLEDVINLVETIYLDDGFVFVDCTLGEGGHSKAVLSKYQNINVIGIERDDVVLNRAKESLVEFSKRISYSN
TWFDDFFCKYSLHRRFNFILADLGISMFHYKVGGRGFSFLEDEKLDMRLFPSDGGISAYDIVNTFDKEMLENLIYELGGE
YYSRRIVKAILEYRKINKIQTSRELQRIICKAYPNVKFKINPATKTFQALRIYVNDELDRLKRSLPLWIANLSKNGILAI
ITFHSLEDKIVKEFFKGLTKDQYCILTKKPITSSFKEKQYNNASRSAKLRAVKKLYE
>Mature_296_residues
GDIFHIPVLLEDVINLVETIYLDDGFVFVDCTLGEGGHSKAVLSKYQNINVIGIERDDVVLNRAKESLVEFSKRISYSNT
WFDDFFCKYSLHRRFNFILADLGISMFHYKVGGRGFSFLEDEKLDMRLFPSDGGISAYDIVNTFDKEMLENLIYELGGEY
YSRRIVKAILEYRKINKIQTSRELQRIICKAYPNVKFKINPATKTFQALRIYVNDELDRLKRSLPLWIANLSKNGILAII
TFHSLEDKIVKEFFKGLTKDQYCILTKKPITSSFKEKQYNNASRSAKLRAVKKLYE

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=343, Percent_Identity=30.3206997084548, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI165377202, Length=205, Percent_Identity=29.7560975609756, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1786270, Length=292, Percent_Identity=32.5342465753425, Blast_Score=139, Evalue=3e-34,
Organism=Drosophila melanogaster, GI62472493, Length=331, Percent_Identity=29.9093655589124, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI28571637, Length=331, Percent_Identity=29.9093655589124, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_BORDL (B5RLD2)

Other databases:

- EMBL:   CP000976
- RefSeq:   YP_002221967.1
- ProteinModelPortal:   B5RLD2
- EnsemblBacteria:   EBBORT00000001471
- GeneID:   6918393
- GenomeReviews:   CP000976_GR
- KEGG:   bdu:BDU_309
- GeneTree:   EBGT00050000007203
- HOGENOM:   HBG302779
- OMA:   RPINSTL
- ProtClustDB:   CLSK507503
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 34518; Mature: 34387

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: NA

Important sites: BINDING 56-56 BINDING 87-87 BINDING 102-102 BINDING 109-109

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDIFHIPVLLEDVINLVETIYLDDGFVFVDCTLGEGGHSKAVLSKYQNINVIGIERDDV
CCCEEEHHHHHHHHHHHHHHHEECCCEEEEEEEECCCCCHHHHHHHHCCCEEEEECCCHH
VLNRAKESLVEFSKRISYSNTWFDDFFCKYSLHRRFNFILADLGISMFHYKVGGRGFSFL
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHEEEEECCCCCCCC
EDEKLDMRLFPSDGGISAYDIVNTFDKEMLENLIYELGGEYYSRRIVKAILEYRKINKIQ
CCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
TSRELQRIICKAYPNVKFKINPATKTFQALRIYVNDELDRLKRSLPLWIANLSKNGILAI
HHHHHHHHHHHHCCCCEEEECCCHHHHHHHHEEECHHHHHHHHHCCEEEEECCCCCEEEE
ITFHSLEDKIVKEFFKGLTKDQYCILTKKPITSSFKEKQYNNASRSAKLRAVKKLYE
EEECCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GDIFHIPVLLEDVINLVETIYLDDGFVFVDCTLGEGGHSKAVLSKYQNINVIGIERDDV
CCEEEHHHHHHHHHHHHHHHEECCCEEEEEEEECCCCCHHHHHHHHCCCEEEEECCCHH
VLNRAKESLVEFSKRISYSNTWFDDFFCKYSLHRRFNFILADLGISMFHYKVGGRGFSFL
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHEEEEECCCCCCCC
EDEKLDMRLFPSDGGISAYDIVNTFDKEMLENLIYELGGEYYSRRIVKAILEYRKINKIQ
CCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
TSRELQRIICKAYPNVKFKINPATKTFQALRIYVNDELDRLKRSLPLWIANLSKNGILAI
HHHHHHHHHHHHCCCCEEEECCCHHHHHHHHEEECHHHHHHHHHCCEEEEECCCCCEEEE
ITFHSLEDKIVKEFFKGLTKDQYCILTKKPITSSFKEKQYNNASRSAKLRAVKKLYE
EEECCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA