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The map label for this gene is murF [H]

Identifier: 203284225

GI number: 203284225

Start: 326914

End: 328311

Strand: Reverse

Name: murF [H]

Synonym: BDU_307

Alternate gene names: 203284225

Gene position: 328311-326914 (Counterclockwise)

Preceding gene: 203284226

Following gene: 203284224

Centisome position: 35.24

GC content: 26.82

Gene sequence:

>1398_bases
TTGGTGCATATAAAAATTAAAGATATTTTAGATTCTCTAAAGGATGTTAAATTTATTGGTGATGTTAGTAATGTGCAAAA
AATTGTATCATTTTATTCACTAGATAGTCGTGAGATAAATGTAGAGAATAGTGAAGTTAGTCTTTATTTTGCTTATAAAG
GTGATAGGGTAGATGGATTTTTTTTTGTTAAATATTTAATTGATATTGGTGTTAAATGTTTTGTATGTTCTAAAGATCGG
GAACTTTTGTGTATTGAATATTTAAAAAAAGATAAGGATTTAATTTTTTTGCTAACTACTAATGTTGTAACTTGTCTTCA
AAATTTAGCAGCTCACTTTATTAAAAAAACACGTTTTAAAAGAATAGCTATTACAGGTAGTAATGGAAAGACTACAACGA
AAGAGATGCTTTACAGTATATTGTCAGAGAGATATAAAACTTGCAAAACTTGGGGAAATTTAAATTCAGATATTGGATTG
CCTTTGAGTATTTTAAGAACCAAAGGAGATGAAGAGTATGCTGTTTTTGAAGTAGGAATTAGTTATGTTGGAGAGATGAA
TATCATTGCTGAGATATTAAATCCTGAAATTGTTATTGTAACAAATGTTAGTTATGCTCATATGCAAGCTTTTGAGGATT
TAAAAATTGTTGCTTATGAGAAGAGTAAAATTATTACAAAAGATACCAAAATGTTAATTTTAAATGAAAGTTGTCCTCAG
CATGTATATCTAGAAGAAATAGCCAAATCTGTAAACCCAAATATCAATATCTTTTATTTTGAATTTCATAATCTTCAAAT
TAGATCATTTTCATTTGTAAATGATAAATTTTCTTATGATTTTACTTATAAAGGATGTGATTATTCCATTTTATTACCCG
GGAAACATAATATTTGCAATGCAATATCTTGTATTAATTTAGCTTTATTTCTTGGACTAAGTGAAAATGAAATTCGTAAT
GGTTTGTTACATGCTGATTTTCAAAAGGGTAGAGCAGAACTTGTAAGAATAAAAGATTATTTGGTTTTAAATGATTCTTA
TAATGGTAATTTAAGCTCATTTATGGCTTTGAAAGAAATGATTTTAGATCTTGAGATTAAAGATAGAAAAATCATTGTTC
TTGGGTCTTTTAAAGAACTTGGAAAATTTGCATATGAGGTTCATAAAACAGCTATAGAAGAAGTTGTTTTAATGAATTTT
GATAAGGTTTTTTTAATTGGTGAAGAATTTCAAGCAGTTAAGAGAGTTGATAGTCTATCCTCAAATAGTTGTTTATTTTA
TTTTAATAATTTTGAGAGTTTTATTGATTGTTTTGTAAATGCTTTAGAATCAGGGTGTTTTATTGCGATTAAAGGGTCAA
GGACAAATAGACTTGAGAGAGTGCTTGACTATCTTTGA

Upstream 100 bases:

>100_bases
TATTGATAGAATAGAAGATCTTAGTCAAAATTATTTAGATTTAGAAAAAAAAGATAATAAAGATATTAATATTATTAAAC
GATAATATCTATGAGGATAT

Downstream 100 bases:

>100_bases
ATTAATGGTGGAAGAGAGGATTTATGTTTTGTTTTTTAGGACTTAGATTATTGAAATATATTACTTTTAGAACCGCTTAT
GCTACTATTTTTGCATTTTT

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate-D-alanyl-D-alanine ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MVHIKIKDILDSLKDVKFIGDVSNVQKIVSFYSLDSREINVENSEVSLYFAYKGDRVDGFFFVKYLIDIGVKCFVCSKDR
ELLCIEYLKKDKDLIFLLTTNVVTCLQNLAAHFIKKTRFKRIAITGSNGKTTTKEMLYSILSERYKTCKTWGNLNSDIGL
PLSILRTKGDEEYAVFEVGISYVGEMNIIAEILNPEIVIVTNVSYAHMQAFEDLKIVAYEKSKIITKDTKMLILNESCPQ
HVYLEEIAKSVNPNINIFYFEFHNLQIRSFSFVNDKFSYDFTYKGCDYSILLPGKHNICNAISCINLALFLGLSENEIRN
GLLHADFQKGRAELVRIKDYLVLNDSYNGNLSSFMALKEMILDLEIKDRKIIVLGSFKELGKFAYEVHKTAIEEVVLMNF
DKVFLIGEEFQAVKRVDSLSSNSCLFYFNNFESFIDCFVNALESGCFIAIKGSRTNRLERVLDYL

Sequences:

>Translated_465_residues
MVHIKIKDILDSLKDVKFIGDVSNVQKIVSFYSLDSREINVENSEVSLYFAYKGDRVDGFFFVKYLIDIGVKCFVCSKDR
ELLCIEYLKKDKDLIFLLTTNVVTCLQNLAAHFIKKTRFKRIAITGSNGKTTTKEMLYSILSERYKTCKTWGNLNSDIGL
PLSILRTKGDEEYAVFEVGISYVGEMNIIAEILNPEIVIVTNVSYAHMQAFEDLKIVAYEKSKIITKDTKMLILNESCPQ
HVYLEEIAKSVNPNINIFYFEFHNLQIRSFSFVNDKFSYDFTYKGCDYSILLPGKHNICNAISCINLALFLGLSENEIRN
GLLHADFQKGRAELVRIKDYLVLNDSYNGNLSSFMALKEMILDLEIKDRKIIVLGSFKELGKFAYEVHKTAIEEVVLMNF
DKVFLIGEEFQAVKRVDSLSSNSCLFYFNNFESFIDCFVNALESGCFIAIKGSRTNRLERVLDYL
>Mature_465_residues
MVHIKIKDILDSLKDVKFIGDVSNVQKIVSFYSLDSREINVENSEVSLYFAYKGDRVDGFFFVKYLIDIGVKCFVCSKDR
ELLCIEYLKKDKDLIFLLTTNVVTCLQNLAAHFIKKTRFKRIAITGSNGKTTTKEMLYSILSERYKTCKTWGNLNSDIGL
PLSILRTKGDEEYAVFEVGISYVGEMNIIAEILNPEIVIVTNVSYAHMQAFEDLKIVAYEKSKIITKDTKMLILNESCPQ
HVYLEEIAKSVNPNINIFYFEFHNLQIRSFSFVNDKFSYDFTYKGCDYSILLPGKHNICNAISCINLALFLGLSENEIRN
GLLHADFQKGRAELVRIKDYLVLNDSYNGNLSSFMALKEMILDLEIKDRKIIVLGSFKELGKFAYEVHKTAIEEVVLMNF
DKVFLIGEEFQAVKRVDSLSSNSCLFYFNNFESFIDCFVNALESGCFIAIKGSRTNRLERVLDYL

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=475, Percent_Identity=25.8947368421053, Blast_Score=149, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR005863 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 53350; Mature: 53350

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVHIKIKDILDSLKDVKFIGDVSNVQKIVSFYSLDSREINVENSEVSLYFAYKGDRVDGF
CEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEECCEEEEEEEEECCCCCHH
FFVKYLIDIGVKCFVCSKDRELLCIEYLKKDKDLIFLLTTNVVTCLQNLAAHFIKKTRFK
HHHHHHHHCCCEEEEECCCCCEEEHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCEE
RIAITGSNGKTTTKEMLYSILSERYKTCKTWGNLNSDIGLPLSILRTKGDEEYAVFEVGI
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCEEEEEECH
SYVGEMNIIAEILNPEIVIVTNVSYAHMQAFEDLKIVAYEKSKIITKDTKMLILNESCPQ
HHHCCHHHHHHHCCCCEEEEECCCHHHHHHHCCCEEEEEECCEEEECCCEEEEEECCCCC
HVYLEEIAKSVNPNINIFYFEFHNLQIRSFSFVNDKFSYDFTYKGCDYSILLPGKHNICN
HHHHHHHHHHCCCCEEEEEEEEECEEEEEEEEECCCEEEEEEECCCCEEEEECCCCCHHH
AISCINLALFLGLSENEIRNGLLHADFQKGRAELVRIKDYLVLNDSYNGNLSSFMALKEM
HHHHHHHHHHCCCCHHHHHCCCEECCHHCCHHHEEEEEEEEEEECCCCCCHHHHHHHHHH
ILDLEIKDRKIIVLGSFKELGKFAYEVHKTAIEEVVLMNFDKVFLIGEEFQAVKRVDSLS
HHCEEECCCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHCCC
SNSCLFYFNNFESFIDCFVNALESGCFIAIKGSRTNRLERVLDYL
CCCEEEEECCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCC
>Mature Secondary Structure
MVHIKIKDILDSLKDVKFIGDVSNVQKIVSFYSLDSREINVENSEVSLYFAYKGDRVDGF
CEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEECCEEEEEEEEECCCCCHH
FFVKYLIDIGVKCFVCSKDRELLCIEYLKKDKDLIFLLTTNVVTCLQNLAAHFIKKTRFK
HHHHHHHHCCCEEEEECCCCCEEEHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCEE
RIAITGSNGKTTTKEMLYSILSERYKTCKTWGNLNSDIGLPLSILRTKGDEEYAVFEVGI
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCEEEEEECH
SYVGEMNIIAEILNPEIVIVTNVSYAHMQAFEDLKIVAYEKSKIITKDTKMLILNESCPQ
HHHCCHHHHHHHCCCCEEEEECCCHHHHHHHCCCEEEEEECCEEEECCCEEEEEECCCCC
HVYLEEIAKSVNPNINIFYFEFHNLQIRSFSFVNDKFSYDFTYKGCDYSILLPGKHNICN
HHHHHHHHHHCCCCEEEEEEEEECEEEEEEEEECCCEEEEEEECCCCEEEEECCCCCHHH
AISCINLALFLGLSENEIRNGLLHADFQKGRAELVRIKDYLVLNDSYNGNLSSFMALKEM
HHHHHHHHHHCCCCHHHHHCCCEECCHHCCHHHEEEEEEEEEEECCCCCCHHHHHHHHHH
ILDLEIKDRKIIVLGSFKELGKFAYEVHKTAIEEVVLMNFDKVFLIGEEFQAVKRVDSLS
HHCEEECCCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHCCC
SNSCLFYFNNFESFIDCFVNALESGCFIAIKGSRTNRLERVLDYL
CCCEEEEECCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]