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The map label for this gene is fliF [H]

Identifier: 203284212

GI number: 203284212

Start: 314951

End: 316657

Strand: Reverse

Name: fliF [H]

Synonym: BDU_294

Alternate gene names: 203284212

Gene position: 316657-314951 (Counterclockwise)

Preceding gene: 203284213

Following gene: 203284211

Centisome position: 33.99

GC content: 31.17

Gene sequence:

>1707_bases
TTGAACAATTTTATTACTAAGTTTTTTGCATCAATAAGTAAAAAGATAAAAAAAGCCACTATGGTTCAAAAAGTGGCTTT
TGGGGTTATTGCTTTATTTATAATTTTGTCGCTTGTTTTTTTGGTGAGTTTTTCTACTAGAAAACAAGGTATTGCTCTTT
TTGGTATTTCGATTAAGGATCAGTACTTGTTAGATAAAATAGTGCAGAGATTGGATAGAGAGAATGCAAAGTATACTATT
ACTGCTGATGGAAGAATTTACTTAAGTGATGAAAATATGTCTAAGAGAATAAGAGCAATTCTTGTAAGAGAAGAGCTTAT
TCCTATGCATATGGATCCTTGGTCTCTTTTTGATATTGATAGATGGACTATTACTGATTTTGAGAGGAATATTAATCTTA
GAAGGTCAATTACAAGAGCTGTTGAGCAACATATTGTTGCTCTTGATGATGTTGATGCTGTTAGTATTAATCTTGTTATG
CCAGAAAAAGCTCTTTTTAAAGAATCACAAGAATCTGTGAGAGCGTCTGTTAGAATTACTCCTAAACCTGGTTCTGATAT
TGCAACTAATCGTCAAAAAGTAGAAGGTCTTGTTAAGTTAATTCAATATGCTGTTGAAGGTCTTGAAGCAGATAATATTG
TTATTGTTGATAATAAAGGAACCATTTTAAATGATTTTTCTAATTTAGATGGTGTTGATAGAATTGATTTAGCTGAGAAG
GAAAGAAAACTTAAATTAAAATATGAATCTATGCTTCGTGATGAGATTGATTTTGCTTTAAGTAAGGTTTTGTCTAGTGA
TAGATTTATGATTGCAAGGGTTAATGTTACATTAGATACTTCAAAGCAAACTACAGAATCTAAAGAGTATGCTCCTATTG
AGCTTGAACCTCAAGATCCAAAAGTTTCTTATAATACAAGAAAAGTGAGTGATTCTACATTAATTTCTTCTCAAGTACAT
AAGAGAGAGTATGAAGGACAAGGTTATAGTCCATGGGGTCCTCCTGGACAGGAAGGTAATACTCCTCCTGAATACCGAGA
TATGAGCGATATTATTGGAAAATCAAATGAGTTTAAAGAAATAAAAAATGTTGCTCTTAATGAAAAAAAATCTTTAAGTG
AAAAGGAACCTGCTAGAGTTTCTGGGATTTCGCTTGGAATTTTTATAGATGGTATTTGGGATTTTGTTTATGATGAGTCT
GGAAATTTCGTTATGGAAAATGGGATGCGAAAAAGGGAGTATAAGCCTATTTCTGATGAGACTTTAAAAAGTATAACTGA
TGTTTTGCAGAGTTCTTTTGAGTATAAACCAGAAAGAGGTGATTCAATTACTGTTAGAAATATTATCTTTGATAGATCAA
GTGAATTTAGAAAAATAGATGAAGATTATTTTGCAGGTGAAAAATTTAAATTTCTTATTTTTGTTGTAAGTATAATAATT
GCTTTATTGATATTAATATTTACAGTGTTTTTCATTATTTCTAGAGAACTTGAGAGAAGAAGACGTCTTAGAGAAGAAGA
ATTTGCAAGACAAGCACATTTAAGACGTCAACAGGCATTAATGGATGGTGATAATATTGGGATTGATGATGTTGTTGGTG
GATTTAGAGAAGAGGATGAACTTCAAAGTAATGCTGAAATTTTAGCTAGAGAGAAACCCGAAGATGTTGCTAAGCTTATT
AGGACATGGATTGTGAAGAATGTATAA

Upstream 100 bases:

>100_bases
GCTAAAGCTAATATGAATTTAAGTATTACAAAAGCTGTTGTTGAGAAAAGCATAAAGGCTTATCAAGATGTAATCAATAT
TCGTTAAGGAGCTATGCAGT

Downstream 100 bases:

>100_bases
AGGATAATAGTTTATATGGAAGATCAAAAAGAAAAAGAAATATTTGGTGTTTCTACTTTAACAGGAAAACAAAAGGCTGC
TATTTTATTAGTTTCTGTAG

Product: flagellar MS-ring protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 568; Mature: 568

Protein sequence:

>568_residues
MNNFITKFFASISKKIKKATMVQKVAFGVIALFIILSLVFLVSFSTRKQGIALFGISIKDQYLLDKIVQRLDRENAKYTI
TADGRIYLSDENMSKRIRAILVREELIPMHMDPWSLFDIDRWTITDFERNINLRRSITRAVEQHIVALDDVDAVSINLVM
PEKALFKESQESVRASVRITPKPGSDIATNRQKVEGLVKLIQYAVEGLEADNIVIVDNKGTILNDFSNLDGVDRIDLAEK
ERKLKLKYESMLRDEIDFALSKVLSSDRFMIARVNVTLDTSKQTTESKEYAPIELEPQDPKVSYNTRKVSDSTLISSQVH
KREYEGQGYSPWGPPGQEGNTPPEYRDMSDIIGKSNEFKEIKNVALNEKKSLSEKEPARVSGISLGIFIDGIWDFVYDES
GNFVMENGMRKREYKPISDETLKSITDVLQSSFEYKPERGDSITVRNIIFDRSSEFRKIDEDYFAGEKFKFLIFVVSIII
ALLILIFTVFFIISRELERRRRLREEEFARQAHLRRQQALMDGDNIGIDDVVGGFREEDELQSNAEILAREKPEDVAKLI
RTWIVKNV

Sequences:

>Translated_568_residues
MNNFITKFFASISKKIKKATMVQKVAFGVIALFIILSLVFLVSFSTRKQGIALFGISIKDQYLLDKIVQRLDRENAKYTI
TADGRIYLSDENMSKRIRAILVREELIPMHMDPWSLFDIDRWTITDFERNINLRRSITRAVEQHIVALDDVDAVSINLVM
PEKALFKESQESVRASVRITPKPGSDIATNRQKVEGLVKLIQYAVEGLEADNIVIVDNKGTILNDFSNLDGVDRIDLAEK
ERKLKLKYESMLRDEIDFALSKVLSSDRFMIARVNVTLDTSKQTTESKEYAPIELEPQDPKVSYNTRKVSDSTLISSQVH
KREYEGQGYSPWGPPGQEGNTPPEYRDMSDIIGKSNEFKEIKNVALNEKKSLSEKEPARVSGISLGIFIDGIWDFVYDES
GNFVMENGMRKREYKPISDETLKSITDVLQSSFEYKPERGDSITVRNIIFDRSSEFRKIDEDYFAGEKFKFLIFVVSIII
ALLILIFTVFFIISRELERRRRLREEEFARQAHLRRQQALMDGDNIGIDDVVGGFREEDELQSNAEILAREKPEDVAKLI
RTWIVKNV
>Mature_568_residues
MNNFITKFFASISKKIKKATMVQKVAFGVIALFIILSLVFLVSFSTRKQGIALFGISIKDQYLLDKIVQRLDRENAKYTI
TADGRIYLSDENMSKRIRAILVREELIPMHMDPWSLFDIDRWTITDFERNINLRRSITRAVEQHIVALDDVDAVSINLVM
PEKALFKESQESVRASVRITPKPGSDIATNRQKVEGLVKLIQYAVEGLEADNIVIVDNKGTILNDFSNLDGVDRIDLAEK
ERKLKLKYESMLRDEIDFALSKVLSSDRFMIARVNVTLDTSKQTTESKEYAPIELEPQDPKVSYNTRKVSDSTLISSQVH
KREYEGQGYSPWGPPGQEGNTPPEYRDMSDIIGKSNEFKEIKNVALNEKKSLSEKEPARVSGISLGIFIDGIWDFVYDES
GNFVMENGMRKREYKPISDETLKSITDVLQSSFEYKPERGDSITVRNIIFDRSSEFRKIDEDYFAGEKFKFLIFVVSIII
ALLILIFTVFFIISRELERRRRLREEEFARQAHLRRQQALMDGDNIGIDDVVGGFREEDELQSNAEILAREKPEDVAKLI
RTWIVKNV

Specific function: The M ring may be actively involved in energy transduction [H]

COG id: COG1766

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway lipoprotein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein. Bacterial flagellum basal body (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliF family [H]

Homologues:

Organism=Escherichia coli, GI1788248, Length=413, Percent_Identity=25.4237288135593, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013556
- InterPro:   IPR000067
- InterPro:   IPR006182 [H]

Pfam domain/function: PF01514 YscJ_FliF; PF08345 YscJ_FliF_C [H]

EC number: NA

Molecular weight: Translated: 65294; Mature: 65294

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNFITKFFASISKKIKKATMVQKVAFGVIALFIILSLVFLVSFSTRKQGIALFGISIKD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCH
QYLLDKIVQRLDRENAKYTITADGRIYLSDENMSKRIRAILVREELIPMHMDPWSLFDID
HHHHHHHHHHHCCCCCCEEEEECCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCEEECC
RWTITDFERNINLRRSITRAVEQHIVALDDVDAVSINLVMPEKALFKESQESVRASVRIT
CEEECHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHEEEEEEC
PKPGSDIATNRQKVEGLVKLIQYAVEGLEADNIVIVDNKGTILNDFSNLDGVDRIDLAEK
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEECCCCCCCCCCCCCHHHH
ERKLKLKYESMLRDEIDFALSKVLSSDRFMIARVNVTLDTSKQTTESKEYAPIELEPQDP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCCCCEEECCCCC
KVSYNTRKVSDSTLISSQVHKREYEGQGYSPWGPPGQEGNTPPEYRDMSDIIGKSNEFKE
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHH
IKNVALNEKKSLSEKEPARVSGISLGIFIDGIWDFVYDESGNFVMENGMRKREYKPISDE
HHHHHHCCHHCCCCCCCCEECCEEEEEEHHHHHHHHCCCCCCEEECCCCCHHCCCCCCHH
TLKSITDVLQSSFEYKPERGDSITVRNIIFDRSSEFRKIDEDYFAGEKFKFLIFVVSIII
HHHHHHHHHHHHCCCCCCCCCEEEEEHEEECCCHHHHHCCHHHHCCCHHHHHHHHHHHHH
ALLILIFTVFFIISRELERRRRLREEEFARQAHLRRQQALMDGDNIGIDDVVGGFREEDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHH
LQSNAEILAREKPEDVAKLIRTWIVKNV
HHCCHHHHHCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNNFITKFFASISKKIKKATMVQKVAFGVIALFIILSLVFLVSFSTRKQGIALFGISIKD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCH
QYLLDKIVQRLDRENAKYTITADGRIYLSDENMSKRIRAILVREELIPMHMDPWSLFDID
HHHHHHHHHHHCCCCCCEEEEECCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCEEECC
RWTITDFERNINLRRSITRAVEQHIVALDDVDAVSINLVMPEKALFKESQESVRASVRIT
CEEECHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHEEEEEEC
PKPGSDIATNRQKVEGLVKLIQYAVEGLEADNIVIVDNKGTILNDFSNLDGVDRIDLAEK
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEECCCCCCCCCCCCCHHHH
ERKLKLKYESMLRDEIDFALSKVLSSDRFMIARVNVTLDTSKQTTESKEYAPIELEPQDP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCCCCEEECCCCC
KVSYNTRKVSDSTLISSQVHKREYEGQGYSPWGPPGQEGNTPPEYRDMSDIIGKSNEFKE
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHH
IKNVALNEKKSLSEKEPARVSGISLGIFIDGIWDFVYDESGNFVMENGMRKREYKPISDE
HHHHHHCCHHCCCCCCCCEECCEEEEEEHHHHHHHHCCCCCCEEECCCCCHHCCCCCCHH
TLKSITDVLQSSFEYKPERGDSITVRNIIFDRSSEFRKIDEDYFAGEKFKFLIFVVSIII
HHHHHHHHHHHHCCCCCCCCCEEEEEHEEECCCHHHHHCCHHHHCCCHHHHHHHHHHHHH
ALLILIFTVFFIISRELERRRRLREEEFARQAHLRRQQALMDGDNIGIDDVVGGFREEDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHH
LQSNAEILAREKPEDVAKLIRTWIVKNV
HHCCHHHHHCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]