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The map label for this gene is alaS [H]

Identifier: 203284140

GI number: 203284140

Start: 235600

End: 237378

Strand: Reverse

Name: alaS [H]

Synonym: BDU_221

Alternate gene names: 203284140

Gene position: 237378-235600 (Counterclockwise)

Preceding gene: 203284141

Following gene: 203284139

Centisome position: 25.48

GC content: 30.75

Gene sequence:

>1779_bases
GTGAAACTTGATGAGCTACGTGCAAAATATATAGACTTTTTTAAAAGTAAGGGACATTGTGAGATTGTAGGTAAATCTTT
GATTCCTGATAATGATTCTACGGTTCTTTTTAATACGGCTGGTATGCAGCCCCTTGTACCTTATTTGCTTGGAGAGATTC
ATCCATCTGGAGATATGTTAGTTGATGTTCAAAAATGTTTAAGAACAGGAGATATTGATGAGGTTGGAGATTTAAGTCAT
TTGACTTTTTTTGAGATGCTTGGAAATTGGTCTCTTGGTGCTTATTTTAAAGAACTTTCAGTAGAATATAGTTTTGAGTT
TTTAACTTCACCTCATTATTTAAATATTTCAAAAGATAAACTTTATGTTAGTGTATTTGAAGGTGATAAAGATATTCCTA
GAGATGTAAAAACAGCTGATATTTGGGAAAAACTTGGAATTTCTAAAGATAGAATATTTTATCTCTCAAGAGAGCATAAT
TTTTGGGGTCCTGTTGGGAATGTAGGTCCTTGTGGTCCAGATACCGAGATATTTGTTGATACAGGAATAGATAAATGTTC
TAATACGTGTGATATTACTTGTTCTTGTGGCAAATATTTTGAGATTTGGAATAATGTTTTTATGCAATATAACAAGGATA
ATAATGGTAATTATGAAGAATTAAAGAGGAAATGTGTAGATACAGGCATGGGTATTGAGAGAACAATTACATTTTTACAG
GGAAAATCTTCAGTTTATGATACTGATGCATTTAGACCTATAATCAAACAGATAGAAAAAATTTCTGGTAAGGTTTATGG
GGATAATTTGGAAGATGATAGAGCTATTAGAATCATTGCTGATCATATTAAGGCCAGTTGTTTTATTTTAGCTGATAATT
TTTCAGTTCTTCCATCTAATGTGGGACAAGGTTATGTTTTAAGAAGGCTTATTAGAAGAGCTATTAGATATGCAAAAAAA
CTTGGTATGGAATCCCATTGTTTAGCAGATCTTGTTGATTCTGTTGAAGACATTTATAAATCTTTTTATAAAGAGATAAC
AGAAAAAAAAGATTTTATTAAGAATGAATTGAATATAGAAGAAGAAAAATTTTTTAAGACTTTGCGTTATGGAGAACAAG
AATTTGTTAAATTGATCCATCAACTATCATCAAAATTAATTCCAGGTGATATTTCTTTTAAACTTTATGATACTTATGGT
TTCCCTTATGAAATTACAGAAGAACTTGCAATTGAATATGGGTTTAGTATGGATAGGGAGGGTTTTGAAGAACATTTTAA
AAAACATCAAGAAGTGTCTAAGAAGGGAAGTGATAAGGTATTTAAGGGTGGACTTGCAGATTGTTCATATGAGACTACTA
AATTGCATACAGCTACTCATTTACTTCATAAGGCTCTTCAATTGGTGCTAGGTGATCATGTAAGACAAAAGGGTAGTAAT
ATTACTTCTCAGAGATTAAGATTTGATTTTAGTCATCCTTGTAAAATGACGGATGATGAGATAAAAAAGGTTGAAGATAT
TGTTAATTTACAAATAAAAAATAAATTGTCTGTAAAGAAATCTATAATGAGTTTGGAGGATGCTTTGGTTAAAGGGGCTA
TGGCTTTATTTGGTGAAAAATATGAAGATATTGTAAGTGTATATGAAATAGATGGTTTTTCAATTGAAGTTTGTGGTGGT
CCTCATGTTAATAATACCAGTGAGCTTGGCACTTTTAAGATACAAAAAGAACAAGCTTCTTCATCAGGAATAAGGAGAAT
AAGGGCAATTTTGATATGA

Upstream 100 bases:

>100_bases
AAGATATAAAGGCAATGACTACGTCTTTTGTTAATTATATTAAAGAGTTAACTTTGAAAGGTGAGTTGATTATTTATAGA
AAGAATGAGGAGTTTGTTTA

Downstream 100 bases:

>100_bases
TAATAGAAAAATTATGGAGGATACTTTATGTTTAAGCATTTGTGTGATTATTTATTAACTCTTCATCCTATTTTTTTAGG
GTTTTTGGGTTCTGTTTTTA

Product: alanyl-tRNA synthetase

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 592; Mature: 592

Protein sequence:

>592_residues
MKLDELRAKYIDFFKSKGHCEIVGKSLIPDNDSTVLFNTAGMQPLVPYLLGEIHPSGDMLVDVQKCLRTGDIDEVGDLSH
LTFFEMLGNWSLGAYFKELSVEYSFEFLTSPHYLNISKDKLYVSVFEGDKDIPRDVKTADIWEKLGISKDRIFYLSREHN
FWGPVGNVGPCGPDTEIFVDTGIDKCSNTCDITCSCGKYFEIWNNVFMQYNKDNNGNYEELKRKCVDTGMGIERTITFLQ
GKSSVYDTDAFRPIIKQIEKISGKVYGDNLEDDRAIRIIADHIKASCFILADNFSVLPSNVGQGYVLRRLIRRAIRYAKK
LGMESHCLADLVDSVEDIYKSFYKEITEKKDFIKNELNIEEEKFFKTLRYGEQEFVKLIHQLSSKLIPGDISFKLYDTYG
FPYEITEELAIEYGFSMDREGFEEHFKKHQEVSKKGSDKVFKGGLADCSYETTKLHTATHLLHKALQLVLGDHVRQKGSN
ITSQRLRFDFSHPCKMTDDEIKKVEDIVNLQIKNKLSVKKSIMSLEDALVKGAMALFGEKYEDIVSVYEIDGFSIEVCGG
PHVNNTSELGTFKIQKEQASSSGIRRIRAILI

Sequences:

>Translated_592_residues
MKLDELRAKYIDFFKSKGHCEIVGKSLIPDNDSTVLFNTAGMQPLVPYLLGEIHPSGDMLVDVQKCLRTGDIDEVGDLSH
LTFFEMLGNWSLGAYFKELSVEYSFEFLTSPHYLNISKDKLYVSVFEGDKDIPRDVKTADIWEKLGISKDRIFYLSREHN
FWGPVGNVGPCGPDTEIFVDTGIDKCSNTCDITCSCGKYFEIWNNVFMQYNKDNNGNYEELKRKCVDTGMGIERTITFLQ
GKSSVYDTDAFRPIIKQIEKISGKVYGDNLEDDRAIRIIADHIKASCFILADNFSVLPSNVGQGYVLRRLIRRAIRYAKK
LGMESHCLADLVDSVEDIYKSFYKEITEKKDFIKNELNIEEEKFFKTLRYGEQEFVKLIHQLSSKLIPGDISFKLYDTYG
FPYEITEELAIEYGFSMDREGFEEHFKKHQEVSKKGSDKVFKGGLADCSYETTKLHTATHLLHKALQLVLGDHVRQKGSN
ITSQRLRFDFSHPCKMTDDEIKKVEDIVNLQIKNKLSVKKSIMSLEDALVKGAMALFGEKYEDIVSVYEIDGFSIEVCGG
PHVNNTSELGTFKIQKEQASSSGIRRIRAILI
>Mature_592_residues
MKLDELRAKYIDFFKSKGHCEIVGKSLIPDNDSTVLFNTAGMQPLVPYLLGEIHPSGDMLVDVQKCLRTGDIDEVGDLSH
LTFFEMLGNWSLGAYFKELSVEYSFEFLTSPHYLNISKDKLYVSVFEGDKDIPRDVKTADIWEKLGISKDRIFYLSREHN
FWGPVGNVGPCGPDTEIFVDTGIDKCSNTCDITCSCGKYFEIWNNVFMQYNKDNNGNYEELKRKCVDTGMGIERTITFLQ
GKSSVYDTDAFRPIIKQIEKISGKVYGDNLEDDRAIRIIADHIKASCFILADNFSVLPSNVGQGYVLRRLIRRAIRYAKK
LGMESHCLADLVDSVEDIYKSFYKEITEKKDFIKNELNIEEEKFFKTLRYGEQEFVKLIHQLSSKLIPGDISFKLYDTYG
FPYEITEELAIEYGFSMDREGFEEHFKKHQEVSKKGSDKVFKGGLADCSYETTKLHTATHLLHKALQLVLGDHVRQKGSN
ITSQRLRFDFSHPCKMTDDEIKKVEDIVNLQIKNKLSVKKSIMSLEDALVKGAMALFGEKYEDIVSVYEIDGFSIEVCGG
PHVNNTSELGTFKIQKEQASSSGIRRIRAILI

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG0013

COG function: function code J; Alanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI109148542, Length=447, Percent_Identity=40.4921700223714, Blast_Score=303, Evalue=2e-82,
Organism=Homo sapiens, GI38569417, Length=461, Percent_Identity=33.6225596529284, Blast_Score=256, Evalue=3e-68,
Organism=Escherichia coli, GI1789048, Length=452, Percent_Identity=37.8318584070796, Blast_Score=305, Evalue=5e-84,
Organism=Caenorhabditis elegans, GI17506981, Length=462, Percent_Identity=39.3939393939394, Blast_Score=314, Evalue=7e-86,
Organism=Caenorhabditis elegans, GI17536681, Length=446, Percent_Identity=37.8923766816144, Blast_Score=282, Evalue=3e-76,
Organism=Saccharomyces cerevisiae, GI6324911, Length=463, Percent_Identity=38.8768898488121, Blast_Score=294, Evalue=2e-80,
Organism=Drosophila melanogaster, GI24582809, Length=458, Percent_Identity=38.4279475982533, Blast_Score=302, Evalue=3e-82,
Organism=Drosophila melanogaster, GI45552267, Length=458, Percent_Identity=38.4279475982533, Blast_Score=302, Evalue=3e-82,
Organism=Drosophila melanogaster, GI24658214, Length=510, Percent_Identity=32.9411764705882, Blast_Score=222, Evalue=5e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 67530; Mature: 67530

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLDELRAKYIDFFKSKGHCEIVGKSLIPDNDSTVLFNTAGMQPLVPYLLGEIHPSGDML
CCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCHH
VDVQKCLRTGDIDEVGDLSHLTFFEMLGNWSLGAYFKELSVEYSFEFLTSPHYLNISKDK
HHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHEEEEEEECCCCEEEECCCE
LYVSVFEGDKDIPRDVKTADIWEKLGISKDRIFYLSREHNFWGPVGNVGPCGPDTEIFVD
EEEEEECCCCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEEE
TGIDKCSNTCDITCSCGKYFEIWNNVFMQYNKDNNGNYEELKRKCVDTGMGIERTITFLQ
CCCHHCCCCCEEEECCCHHHHHHHHHHEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHC
GKSSVYDTDAFRPIIKQIEKISGKVYGDNLEDDRAIRIIADHIKASCFILADNFSVLPSN
CCCCCCCCHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHCCEEEEEECCCEECCCC
VGQGYVLRRLIRRAIRYAKKLGMESHCLADLVDSVEDIYKSFYKEITEKKDFIKNELNIE
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EEKFFKTLRYGEQEFVKLIHQLSSKLIPGDISFKLYDTYGFPYEITEELAIEYGFSMDRE
HHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCHH
GFEEHFKKHQEVSKKGSDKVFKGGLADCSYETTKLHTATHLLHKALQLVLGDHVRQKGSN
HHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ITSQRLRFDFSHPCKMTDDEIKKVEDIVNLQIKNKLSVKKSIMSLEDALVKGAMALFGEK
CCHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YEDIVSVYEIDGFSIEVCGGPHVNNTSELGTFKIQKEQASSSGIRRIRAILI
HHHHHHHHEECCCEEEEECCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLDELRAKYIDFFKSKGHCEIVGKSLIPDNDSTVLFNTAGMQPLVPYLLGEIHPSGDML
CCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCHH
VDVQKCLRTGDIDEVGDLSHLTFFEMLGNWSLGAYFKELSVEYSFEFLTSPHYLNISKDK
HHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHEEEEEEECCCCEEEECCCE
LYVSVFEGDKDIPRDVKTADIWEKLGISKDRIFYLSREHNFWGPVGNVGPCGPDTEIFVD
EEEEEECCCCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEEE
TGIDKCSNTCDITCSCGKYFEIWNNVFMQYNKDNNGNYEELKRKCVDTGMGIERTITFLQ
CCCHHCCCCCEEEECCCHHHHHHHHHHEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHC
GKSSVYDTDAFRPIIKQIEKISGKVYGDNLEDDRAIRIIADHIKASCFILADNFSVLPSN
CCCCCCCCHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHCCEEEEEECCCEECCCC
VGQGYVLRRLIRRAIRYAKKLGMESHCLADLVDSVEDIYKSFYKEITEKKDFIKNELNIE
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EEKFFKTLRYGEQEFVKLIHQLSSKLIPGDISFKLYDTYGFPYEITEELAIEYGFSMDRE
HHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCHH
GFEEHFKKHQEVSKKGSDKVFKGGLADCSYETTKLHTATHLLHKALQLVLGDHVRQKGSN
HHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ITSQRLRFDFSHPCKMTDDEIKKVEDIVNLQIKNKLSVKKSIMSLEDALVKGAMALFGEK
CCHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YEDIVSVYEIDGFSIEVCGGPHVNNTSELGTFKIQKEQASSSGIRRIRAILI
HHHHHHHHEECCCEEEEECCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA