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The map label for this gene is yciL [C]

Identifier: 203284055

GI number: 203284055

Start: 136861

End: 137607

Strand: Reverse

Name: yciL [C]

Synonym: BDU_133

Alternate gene names: 203284055

Gene position: 137607-136861 (Counterclockwise)

Preceding gene: 203284056

Following gene: 203284054

Centisome position: 14.77

GC content: 28.92

Gene sequence:

>747_bases
TTGACGAATATAAACAGCAAAGGGCTTAGAGTACATGTTTTTTTGGCAGAAAAAGGTATAGGTTCTAGGAGGTTTTGCGA
AGAGCTTATAAGAAAAAATCTTATCAAAGTAAATGGCATTTTTGCTAAGCTTGGAGATAAAGTTTTCTTGGGTGATAGAG
TGGAGTATGGAAAACAAGTGTTTGTTTTTCAAGATTCTAAAGTTGTAAATAAAATTTATATTGCTCTTAATAAACCTATA
AATTACCTTTGTTCAAATTTTGATCCAATGGGAAGAAAATTGGCAATATCTTTGGTTCAGCCTTTATTTAGAGAACGTTT
ATTTTCAATTGGCAGGCTTGATTTTAAAAGTTCTGGTCTTTTGCTTTTTACTAATGATGGTCGGTTTGCAAATAGTATTG
TGCATCCAAAGAATGAAATAGAAAAGGAATATATTATTGAATCAAAAAGGGTTGTTAGTGAAGATTTGCTTGTTAATTTT
AAGAATGGAATAAAAGTTGATAATGAACTTTTTAAGTTAAAGTCTTATGTTATGCTTGGTAATAATACAGCCAGGTTAAT
TTTAACAGAAGGTAAAAATAGAGAAATTCGTAAAGTTTTTTTAAGTAGGAATATCTTTTTAAAGAAAGTGCATAGAGTTA
GGATAGGTAATATTACATTGAGTAATTTGAAAGAAGGACAAATTAAGGTTTTGTCTTTAGCTAAAATTAATAAATTAAAA
ACTCAGTTTTTGGGGGCATTGATGTGA

Upstream 100 bases:

>100_bases
AGATTTTGTTATTTTTTAATCGTTCTTGAATATAAAATGTCAAATGTTATAGATTTGGTTTATGTAAATAATGAATTAAT
TGTAATAAAGGGAAGAGGTA

Downstream 100 bases:

>100_bases
TAATAGCAATTGATGGACCTTCAGCTTCAGGAAAAAGTTCTGTTTCAAAAGCATTGAGTATGAAATTGGGTTTTAAATTT
ATTAGTTCTGGTTATTTGTA

Product: pseudouridylate synthase

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MTNINSKGLRVHVFLAEKGIGSRRFCEELIRKNLIKVNGIFAKLGDKVFLGDRVEYGKQVFVFQDSKVVNKIYIALNKPI
NYLCSNFDPMGRKLAISLVQPLFRERLFSIGRLDFKSSGLLLFTNDGRFANSIVHPKNEIEKEYIIESKRVVSEDLLVNF
KNGIKVDNELFKLKSYVMLGNNTARLILTEGKNREIRKVFLSRNIFLKKVHRVRIGNITLSNLKEGQIKVLSLAKINKLK
TQFLGALM

Sequences:

>Translated_248_residues
MTNINSKGLRVHVFLAEKGIGSRRFCEELIRKNLIKVNGIFAKLGDKVFLGDRVEYGKQVFVFQDSKVVNKIYIALNKPI
NYLCSNFDPMGRKLAISLVQPLFRERLFSIGRLDFKSSGLLLFTNDGRFANSIVHPKNEIEKEYIIESKRVVSEDLLVNF
KNGIKVDNELFKLKSYVMLGNNTARLILTEGKNREIRKVFLSRNIFLKKVHRVRIGNITLSNLKEGQIKVLSLAKINKLK
TQFLGALM
>Mature_247_residues
TNINSKGLRVHVFLAEKGIGSRRFCEELIRKNLIKVNGIFAKLGDKVFLGDRVEYGKQVFVFQDSKVVNKIYIALNKPIN
YLCSNFDPMGRKLAISLVQPLFRERLFSIGRLDFKSSGLLLFTNDGRFANSIVHPKNEIEKEYIIESKRVVSEDLLVNFK
NGIKVDNELFKLKSYVMLGNNTARLILTEGKNREIRKVFLSRNIFLKKVHRVRIGNITLSNLKEGQIKVLSLAKINKLKT
QFLGALM

Specific function: Unknown

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=235, Percent_Identity=33.1914893617021, Blast_Score=124, Evalue=5e-30,
Organism=Escherichia coli, GI1788510, Length=232, Percent_Identity=29.3103448275862, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1790453, Length=224, Percent_Identity=29.9107142857143, Blast_Score=99, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70

Molecular weight: Translated: 28421; Mature: 28290

Theoretical pI: Translated: 10.88; Mature: 10.88

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNINSKGLRVHVFLAEKGIGSRRFCEELIRKNLIKVNGIFAKLGDKVFLGDRVEYGKQV
CCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCEEHHHHHHHCCCEEEECCCCCCCCEE
FVFQDSKVVNKIYIALNKPINYLCSNFDPMGRKLAISLVQPLFRERLFSIGRLDFKSSGL
EEEECCHHEEEEEEEECCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
LLFTNDGRFANSIVHPKNEIEKEYIIESKRVVSEDLLVNFKNGIKVDNELFKLKSYVMLG
EEEECCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHEEEEC
NNTARLILTEGKNREIRKVFLSRNIFLKKVHRVRIGNITLSNLKEGQIKVLSLAKINKLK
CCCEEEEEECCCCHHHHHHHHHCCHHHHHHHHEEECCEEECCCCCCCEEEEEHHHHHHHH
TQFLGALM
HHHHHHCC
>Mature Secondary Structure 
TNINSKGLRVHVFLAEKGIGSRRFCEELIRKNLIKVNGIFAKLGDKVFLGDRVEYGKQV
CCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCEEHHHHHHHCCCEEEECCCCCCCCEE
FVFQDSKVVNKIYIALNKPINYLCSNFDPMGRKLAISLVQPLFRERLFSIGRLDFKSSGL
EEEECCHHEEEEEEEECCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
LLFTNDGRFANSIVHPKNEIEKEYIIESKRVVSEDLLVNFKNGIKVDNELFKLKSYVMLG
EEEECCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHEEEEC
NNTARLILTEGKNREIRKVFLSRNIFLKKVHRVRIGNITLSNLKEGQIKVLSLAKINKLK
CCCEEEEEECCCCHHHHHHHHHCCHHHHHHHHEEECCEEECCCCCCCEEEEEHHHHHHHH
TQFLGALM
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]