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The map label for this gene is spoU [C]

Identifier: 203283978

GI number: 203283978

Start: 59553

End: 60209

Strand: Reverse

Name: spoU [C]

Synonym: BDU_56

Alternate gene names: 203283978

Gene position: 60209-59553 (Counterclockwise)

Preceding gene: 203283980

Following gene: 203283975

Centisome position: 6.46

GC content: 28.16

Gene sequence:

>657_bases
ATGGTCAGTGATACATTGAAAAGAATAGAAATATTGGCTGAATTTATAACTCGAAGAAGACGAGAGAGAATAGATGAAGT
ATTAAATAATCGTACGAATTATTTGACACTTGTACTTGAAGATATTTTTCAATCTCAAAATGCAAGTGCTACAATGCGTA
CAATTGAGATTTTAGGACTTAGTAATATTCATATTATTGAGACTAATAATAAGTATACTTTAAATCCAGGTGTTGTTCTT
GGAGCTTCAAAGTGGATAGGTATTAATAAATATAAATGTGTAAAAGTTGCATTTGAACATTTAAAAGCTAATGGGTATAA
AATAGTAGCTACATCATTAGATAATGAAGCCATATCTCTTGAAGATTTTCCAATTGATACTAAAATGGCAGTATTTTTTG
GAACAGAGTTAACGGGACTTAGCCATACAATTTTGCAAAATGCTGATTTGCATTTAAAAATACCAATGTATGGATTTACA
CAAAGTTATAATCTCTCTGTAGCTGTAGCTATAGTCTTTTATTCTTTAATTAATCGTTTAAGAAGTAGTTCTATTGATTA
TTTGCTCAATGAAGATGAAAAGTTAAACTTAAAGCTTGAATATTATAGGAAAATTATAAAAAGGTATAAGGTCATTGAGA
AATTGCAGTTTTTCTAA

Upstream 100 bases:

>100_bases
TCTTCAATTTTTACTTCCACATTTAATATTATATAGAAAATTAATGATTTTGTAATATTTTAATTTGATTTTATTGGTTT
TTTATTTAGAATAATATGTT

Downstream 100 bases:

>100_bases
TTTTTAGTTTGTTTAGGAAACAATATATAAAGGTATCAATTATCATTATTGCAATGGTAAAGATCCATGAACTTTTTACC
ATATTAATGTTATTTCCAGG

Product: spoU protein

Products: S-adenosyl-L-homocysteine; tRNATyr Containing 2'-O-methylguanosine [C]

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MVSDTLKRIEILAEFITRRRRERIDEVLNNRTNYLTLVLEDIFQSQNASATMRTIEILGLSNIHIIETNNKYTLNPGVVL
GASKWIGINKYKCVKVAFEHLKANGYKIVATSLDNEAISLEDFPIDTKMAVFFGTELTGLSHTILQNADLHLKIPMYGFT
QSYNLSVAVAIVFYSLINRLRSSSIDYLLNEDEKLNLKLEYYRKIIKRYKVIEKLQFF

Sequences:

>Translated_218_residues
MVSDTLKRIEILAEFITRRRRERIDEVLNNRTNYLTLVLEDIFQSQNASATMRTIEILGLSNIHIIETNNKYTLNPGVVL
GASKWIGINKYKCVKVAFEHLKANGYKIVATSLDNEAISLEDFPIDTKMAVFFGTELTGLSHTILQNADLHLKIPMYGFT
QSYNLSVAVAIVFYSLINRLRSSSIDYLLNEDEKLNLKLEYYRKIIKRYKVIEKLQFF
>Mature_218_residues
MVSDTLKRIEILAEFITRRRRERIDEVLNNRTNYLTLVLEDIFQSQNASATMRTIEILGLSNIHIIETNNKYTLNPGVVL
GASKWIGINKYKCVKVAFEHLKANGYKIVATSLDNEAISLEDFPIDTKMAVFFGTELTGLSHTILQNADLHLKIPMYGFT
QSYNLSVAVAIVFYSLINRLRSSSIDYLLNEDEKLNLKLEYYRKIIKRYKVIEKLQFF

Specific function: Specifically Methylates Guanosine-18 In Various tRNAs. [C]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=186, Percent_Identity=30.1075268817204, Blast_Score=99, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.34 [C]

Molecular weight: Translated: 25194; Mature: 25194

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSDTLKRIEILAEFITRRRRERIDEVLNNRTNYLTLVLEDIFQSQNASATMRTIEILGL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCCCCHHEEEEEEEEC
SNIHIIETNNKYTLNPGVVLGASKWIGINKYKCVKVAFEHLKANGYKIVATSLDNEAISL
CCEEEEECCCEEEECCCEEEECCCEECCCCHHHHHHHHHHHCCCCEEEEEEECCCCEEEE
EDFPIDTKMAVFFGTELTGLSHTILQNADLHLKIPMYGFTQSYNLSVAVAIVFYSLINRL
ECCCCCCEEEEEECCCCCCCHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHH
RSSSIDYLLNEDEKLNLKLEYYRKIIKRYKVIEKLQFF
HHCCCCEEECCCCEEEEEHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVSDTLKRIEILAEFITRRRRERIDEVLNNRTNYLTLVLEDIFQSQNASATMRTIEILGL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCCCCHHEEEEEEEEC
SNIHIIETNNKYTLNPGVVLGASKWIGINKYKCVKVAFEHLKANGYKIVATSLDNEAISL
CCEEEEECCCEEEECCCEEEECCCEECCCCHHHHHHHHHHHCCCCEEEEEEECCCCEEEE
EDFPIDTKMAVFFGTELTGLSHTILQNADLHLKIPMYGFTQSYNLSVAVAIVFYSLINRL
ECCCCCCEEEEEECCCCCCCHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHH
RSSSIDYLLNEDEKLNLKLEYYRKIIKRYKVIEKLQFF
HHCCCCEEECCCCEEEEEHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; tRNATyr [C]

Specific reaction: S-Adenosyl-L-methionine + tRNATyr = S-adenosyl-L-homocysteine + tRNATyr Containing 2'-O-methylguanosine [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]