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The map label for this gene is CobS

Identifier: 20095107

GI number: 20095107

Start: 1671733

End: 1672458

Strand: Reverse

Name: CobS

Synonym: MK1671

Alternate gene names: 20095107

Gene position: 1672458-1671733 (Counterclockwise)

Preceding gene: 20095108

Following gene: 20095106

Centisome position: 98.67

GC content: 61.43

Gene sequence:

>726_bases
GTGATTCGGGTGGAGTTCCTGAAAGTCTTCAGATTCTTGACCGTCCTTCCGATCGGAGAACACCCGAAGAGTCCGCGTGA
GATCGGCGAGCAGGCATGGTTAGGGCTTCCAGCGGTCGGACTAGTCAGCGGGTTGCTGGCGGGGGTCGTGGCGTGGGCCT
TCGCCGGAACACCGGTTAGAGGTTGCTTGGTAGTTTTGACGCTGCTCGTACTCGAGGGGGCACAGCACTTCGATGGCCTC
GTAGATGTGGGCGACGCGCTGATGGCGGGCGTGATCTCCGAAGAAGGTGCCACGAAGGCTATGCGTGATCCGAGGGTAGG
AGTGGGGGGCTTAGCGATTGGGTCTATGGCACTCCTCCTAGCGGTCGCGTCGTTCGGGTGGATACCCTTCGAGGTGCTGG
TACCCATCGAGGTGTTCTCACGTTTCACCGTACTCCCTATGGCGGCGGTGGGTGAGCCCGCGCCCGCCAGCTACTCCGGT
AGGGTGTTCACGGAGTACGTGGACGCCGATCAGGTATTGCTGGGAGGAATCCTATCAACGGTCGTGTCATTGCCGTTTTC
ACCCGTAGCCACCCTAACATGTGCGGTCTGCTCGGCGGTGGTCGCTTGGACCTGCCTGGAGGCGGCACGCCGTACCATAA
GGGGCGTGAACGGTGACTTCCTGGGTGCGTCAATATGGGTGTCACGGGTCCTGAGCGCCGTTTGCCTAAGCTCCCTGCCC
TGGTGA

Upstream 100 bases:

>100_bases
AGCCCGGAATCCTGAAGGAAGTCGATGAGAGATGGGTTATGCTGGATGACGTCCTTGCAGGTCTCGTTGCCGGTGCCATG
ACCGCCGCGTTCCGCAGGGG

Downstream 100 bases:

>100_bases
TGGCCGGTGGTCGGGCGAAGCGCATGGGTGGGGTGGAGAAGCCGGCCGTAGAAGTGGCGGGTAAACCGCTGCTCGTGTGG
GTGCTCGAGGCACTTCAGGA

Product: cobalamin-5-phosphate synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MIRVEFLKVFRFLTVLPIGEHPKSPREIGEQAWLGLPAVGLVSGLLAGVVAWAFAGTPVRGCLVVLTLLVLEGAQHFDGL
VDVGDALMAGVISEEGATKAMRDPRVGVGGLAIGSMALLLAVASFGWIPFEVLVPIEVFSRFTVLPMAAVGEPAPASYSG
RVFTEYVDADQVLLGGILSTVVSLPFSPVATLTCAVCSAVVAWTCLEAARRTIRGVNGDFLGASIWVSRVLSAVCLSSLP
W

Sequences:

>Translated_241_residues
MIRVEFLKVFRFLTVLPIGEHPKSPREIGEQAWLGLPAVGLVSGLLAGVVAWAFAGTPVRGCLVVLTLLVLEGAQHFDGL
VDVGDALMAGVISEEGATKAMRDPRVGVGGLAIGSMALLLAVASFGWIPFEVLVPIEVFSRFTVLPMAAVGEPAPASYSG
RVFTEYVDADQVLLGGILSTVVSLPFSPVATLTCAVCSAVVAWTCLEAARRTIRGVNGDFLGASIWVSRVLSAVCLSSLP
W
>Mature_241_residues
MIRVEFLKVFRFLTVLPIGEHPKSPREIGEQAWLGLPAVGLVSGLLAGVVAWAFAGTPVRGCLVVLTLLVLEGAQHFDGL
VDVGDALMAGVISEEGATKAMRDPRVGVGGLAIGSMALLLAVASFGWIPFEVLVPIEVFSRFTVLPMAAVGEPAPASYSG
RVFTEYVDADQVLLGGILSTVVSLPFSPVATLTCAVCSAVVAWTCLEAARRTIRGVNGDFLGASIWVSRVLSAVCLSSLP
W

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin)

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBS_METKA (Q8TUT2)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614954.1
- ProteinModelPortal:   Q8TUT2
- GeneID:   1478266
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK1671
- NMPDR:   fig|190192.1.peg.1667
- BioCyc:   MKAN190192:MK1671-MONOMER
- HAMAP:   MF_00719
- InterPro:   IPR003805

Pfam domain/function: PF02654 CobS

EC number: 2.7.8.26

Molecular weight: Translated: 25264; Mature: 25264

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xdd7ac24)-; HASH(0xf2c9c84)-; HASH(0xdb0cd18)-; HASH(0xf02491c)-;

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRVEFLKVFRFLTVLPIGEHPKSPREIGEQAWLGLPAVGLVSGLLAGVVAWAFAGTPVR
CCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHH
GCLVVLTLLVLEGAQHFDGLVDVGDALMAGVISEEGATKAMRDPRVGVGGLAIGSMALLL
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHHHH
AVASFGWIPFEVLVPIEVFSRFTVLPMAAVGEPAPASYSGRVFTEYVDADQVLLGGILST
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHH
VVSLPFSPVATLTCAVCSAVVAWTCLEAARRTIRGVNGDFLGASIWVSRVLSAVCLSSLP
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
W
H
>Mature Secondary Structure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H

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11930014