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The map label for this gene is TpiA

Identifier: 20095100

GI number: 20095100

Start: 1666501

End: 1667181

Strand: Reverse

Name: TpiA

Synonym: MK1664

Alternate gene names: 20095100

Gene position: 1667181-1666501 (Counterclockwise)

Preceding gene: 20095101

Following gene: 20095099

Centisome position: 98.36

GC content: 63.73

Gene sequence:

>681_bases
ATGTTGCGTGTACCTCCTGTTATCGTGAATTTCAAGGCGTACTCGGAAGCGGTCGGTGAGAACGCACTACGACTGGCTCG
AGTGGCCGCGGAAGTGTCGGAAGAAACCGGAGTCGAGGTCGGGATCTGTCCTCCTCATGTCGACCTGCGCGACGTGGTTA
GGGAGGTAGGCGACGAAGTCACGGTCCTAGCGCAGGCAGTTGATGCGGCCGAGCCCGGCGGGAGGACCGGGCACGTGACT
CCCGAGATGGTGGTCGAAGCCGGCGCCGACGGGACGCTTCTGAACCACTCGGAACGCCGGATGCTGTTGGAGGATCTTAA
GGACGTCTGCCGAGCGTGCATAAACGAGGGTCTCCTCACGATCGTGTGTGCGAGCGACGCGCTCGCCGCCCGTGCGGCCG
GGGCCCTCTCGCCTCACGCGGTGGCCGTGGAGCCGCCGGAGCTCATCGGTACGGGGACCCCGGTGTCGAAGGCCGACCCC
GAGGTAGTCGAGCGATCGGTCGAGGTCGTGAAGGAGGTCTCGGAGGAGACGGCCGTACTGTGCGGGGCCGGGATCACCGA
TGGGTCCGATGTGCGTGCCGCCGTGGAATTGGGTGCTGATGGCGTCTTAGTAGCTTCTGGAGTGGTATTAGCGGACGATC
CTAAGGAGGCGTTATTAGACCTCATCTCCGGCCTAGAATAG

Upstream 100 bases:

>100_bases
TCGAACGCGAGTTGAACTCCGTTCCCGGCGTAGTTGAATGCGGCGTATTCCCCGGGGACAAGGTAGATAGGATCATCGTC
GGGAGGTCGTAGGGGGTGAG

Downstream 100 bases:

>100_bases
GGGATGATGGGCATTGGATCGCATACGTAAAATTATGCGGAGGGAGGCCGAGGTAATAGAGTATGCGTGCAACAATCTGC
CCGAAGAAGATGTTAAAAAA

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MLRVPPVIVNFKAYSEAVGENALRLARVAAEVSEETGVEVGICPPHVDLRDVVREVGDEVTVLAQAVDAAEPGGRTGHVT
PEMVVEAGADGTLLNHSERRMLLEDLKDVCRACINEGLLTIVCASDALAARAAGALSPHAVAVEPPELIGTGTPVSKADP
EVVERSVEVVKEVSEETAVLCGAGITDGSDVRAAVELGADGVLVASGVVLADDPKEALLDLISGLE

Sequences:

>Translated_226_residues
MLRVPPVIVNFKAYSEAVGENALRLARVAAEVSEETGVEVGICPPHVDLRDVVREVGDEVTVLAQAVDAAEPGGRTGHVT
PEMVVEAGADGTLLNHSERRMLLEDLKDVCRACINEGLLTIVCASDALAARAAGALSPHAVAVEPPELIGTGTPVSKADP
EVVERSVEVVKEVSEETAVLCGAGITDGSDVRAAVELGADGVLVASGVVLADDPKEALLDLISGLE
>Mature_226_residues
MLRVPPVIVNFKAYSEAVGENALRLARVAAEVSEETGVEVGICPPHVDLRDVVREVGDEVTVLAQAVDAAEPGGRTGHVT
PEMVVEAGADGTLLNHSERRMLLEDLKDVCRACINEGLLTIVCASDALAARAAGALSPHAVAVEPPELIGTGTPVSKADP
EVVERSVEVVKEVSEETAVLCGAGITDGSDVRAAVELGADGVLVASGVVLADDPKEALLDLISGLE

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

None

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_METKA (Q8TUT9)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614947.1
- ProteinModelPortal:   Q8TUT9
- SMR:   Q8TUT9
- GeneID:   1478259
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK1664
- NMPDR:   fig|190192.1.peg.1660
- HOGENOM:   HBG297256
- OMA:   VEPPELI
- ProtClustDB:   PRK04302
- BioCyc:   MKAN190192:MK1664-MONOMER
- BRENDA:   5.3.1.1
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_A
- InterPro:   IPR013785
- InterPro:   IPR000652
- InterPro:   IPR022891
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 23283; Mature: 23283

Theoretical pI: Translated: 4.08; Mature: 4.08

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 96-96 ACT_SITE 144-144 BINDING 10-10 BINDING 12-12

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRVPPVIVNFKAYSEAVGENALRLARVAAEVSEETGVEVGICPPHVDLRDVVREVGDEV
CCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHCCHH
TVLAQAVDAAEPGGRTGHVTPEMVVEAGADGTLLNHSERRMLLEDLKDVCRACINEGLLT
HHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHHHHCCCEEE
IVCASDALAARAAGALSPHAVAVEPPELIGTGTPVSKADPEVVERSVEVVKEVSEETAVL
EEECCHHHHHHHHCCCCCCEEEECCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEE
CGAGITDGSDVRAAVELGADGVLVASGVVLADDPKEALLDLISGLE
ECCCCCCCCHHHHHHHHCCCCEEEECCEEEECCHHHHHHHHHHCCC
>Mature Secondary Structure
MLRVPPVIVNFKAYSEAVGENALRLARVAAEVSEETGVEVGICPPHVDLRDVVREVGDEV
CCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHCCHH
TVLAQAVDAAEPGGRTGHVTPEMVVEAGADGTLLNHSERRMLLEDLKDVCRACINEGLLT
HHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHHHHCCCEEE
IVCASDALAARAAGALSPHAVAVEPPELIGTGTPVSKADPEVVERSVEVVKEVSEETAVL
EEECCHHHHHHHHCCCCCCEEEECCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEE
CGAGITDGSDVRAAVELGADGVLVASGVVLADDPKEALLDLISGLE
ECCCCCCCCHHHHHHHHCCCCEEEECCEEEECCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014