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The map label for this gene is Eno

Identifier: 20095083

GI number: 20095083

Start: 1647793

End: 1649076

Strand: Direct

Name: Eno

Synonym: MK1647

Alternate gene names: 20095083

Gene position: 1647793-1649076 (Clockwise)

Preceding gene: 20095080

Following gene: 20095089

Centisome position: 97.22

GC content: 63.86

Gene sequence:

>1284_bases
ATGCCCAGAATAGTCTCCGTGAAGGCCAGGGAAGTGTTAGACTCACGCGGAGAACCCACGGTGGAAGTGGAGGTGGAACT
CGAAGACGGTACCGTCGGTCGGGCCATGGTCCCATCCGGAGCTTCCACGGGCACGTACGAAGCGCTGGAGCTGAGGGACG
GCGACGATCGTTACGGAGGTAAGGGAGTACGCCGTGCCGTCAGGAACGTGGAGGAGATCATCGCCCCGGAGATCGAGGGA
CTCGACGCGACGGCCCAACCTGACATCGACCGCACGATGATCGAGCTCGACGGCACCGAGAACAAGTCTCACCTCGGAGC
GAACGCGATCCTGGGAGTCTCGCTGGCGGTCGCCCGGGCGGCGGCCAAGTCGCTGGGGATACCGTTGTACCGATACCTAG
GTGGTCCGACGGCGCGCCGACTGCCCGTGCCGTTCATGAACGTGATCAACGGCGGCGAGCACGCGGGTAACGAACTCGAC
TTCCAGGAGCACATGATCGTCCCGCACGGCTTCGAGTCGTTCTCCGAGGCTCTTCGAGCCGGTGTGGAGACGTACCACGT
CCTCGGGGAGCTACTGGAGGAGGAGTACGGACCTATCGCCACGAACGTGGGTGACGAGGGCGGTTACGCTCCACCGATGA
AGGATACGGTGGAACCACTGGACGTCCTCGTGGAGGCCATCGAGGAGGCCGGATACGCACCCGGCAAGGAGATCGCGTTG
GCCCTGGACGCAGCCGCGAGCGAGTTCTACGACGAGGACTCGGGGACGTACAGGGCGTACGGTCAGAAGTATACCCGCGA
CGAGCTGATCGACGTGTACAAGGATCTGGTAAGCCAGTACCCGATCGTTTCCATAGAGGACCCGCTGCACGAGGAGGACT
TCCGGGGCTTCGCCAAGATCACCGAGGAGCTAGGCGATAAGGTCCAAATCGTGGGTGACGACCTGTTCGTGACGAACCCG
GACCGCCTGCGGAAGGGGATCGAAATGGGGGCGGCGAACGCCCTGCTCCTGAAGGTGAACCAGATCGGAACCCTGACGGA
GGCCGTGGAGGCGGGTGAGCTCGCGCTTCAACACGGGTACGGTGTGATGGTGAGCCACCGGAGCGGCGACACCGAGGACC
CGTTCATCGCCGACCTGGCCGTGGCCCTAGGGTGCGGACAGATCAAGACCGGAGCGCCCGCCCGTAGCTCTAGGACGGCC
AAGTACAACCGCCTGCTCCGGATCGAGGAAGACTTAGCCGGGGCGGCGGAGTTCGGACCCCGGAACGACTTCTTCCTCCC
CTAA

Upstream 100 bases:

>100_bases
AAGTGCGTTAAACCCCACCGAAGTCCGCCGCTGCCCCCGCGACACCATGAACGGCAACGGTTTAACCCGCGGTGCGATCG
TAAGGCGGGGGTTCGGGAGC

Downstream 100 bases:

>100_bases
CTCTCACTCTCCGGTATCCACACCTTCGACACCACTTCTCGAGCCATCTTAGACCAGCTCAGGAAATACACGGTCCACAG
AACGGGCGTAGCCGTCGCTA

Product: enolase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MPRIVSVKAREVLDSRGEPTVEVEVELEDGTVGRAMVPSGASTGTYEALELRDGDDRYGGKGVRRAVRNVEEIIAPEIEG
LDATAQPDIDRTMIELDGTENKSHLGANAILGVSLAVARAAAKSLGIPLYRYLGGPTARRLPVPFMNVINGGEHAGNELD
FQEHMIVPHGFESFSEALRAGVETYHVLGELLEEEYGPIATNVGDEGGYAPPMKDTVEPLDVLVEAIEEAGYAPGKEIAL
ALDAAASEFYDEDSGTYRAYGQKYTRDELIDVYKDLVSQYPIVSIEDPLHEEDFRGFAKITEELGDKVQIVGDDLFVTNP
DRLRKGIEMGAANALLLKVNQIGTLTEAVEAGELALQHGYGVMVSHRSGDTEDPFIADLAVALGCGQIKTGAPARSSRTA
KYNRLLRIEEDLAGAAEFGPRNDFFLP

Sequences:

>Translated_427_residues
MPRIVSVKAREVLDSRGEPTVEVEVELEDGTVGRAMVPSGASTGTYEALELRDGDDRYGGKGVRRAVRNVEEIIAPEIEG
LDATAQPDIDRTMIELDGTENKSHLGANAILGVSLAVARAAAKSLGIPLYRYLGGPTARRLPVPFMNVINGGEHAGNELD
FQEHMIVPHGFESFSEALRAGVETYHVLGELLEEEYGPIATNVGDEGGYAPPMKDTVEPLDVLVEAIEEAGYAPGKEIAL
ALDAAASEFYDEDSGTYRAYGQKYTRDELIDVYKDLVSQYPIVSIEDPLHEEDFRGFAKITEELGDKVQIVGDDLFVTNP
DRLRKGIEMGAANALLLKVNQIGTLTEAVEAGELALQHGYGVMVSHRSGDTEDPFIADLAVALGCGQIKTGAPARSSRTA
KYNRLLRIEEDLAGAAEFGPRNDFFLP
>Mature_426_residues
PRIVSVKAREVLDSRGEPTVEVEVELEDGTVGRAMVPSGASTGTYEALELRDGDDRYGGKGVRRAVRNVEEIIAPEIEGL
DATAQPDIDRTMIELDGTENKSHLGANAILGVSLAVARAAAKSLGIPLYRYLGGPTARRLPVPFMNVINGGEHAGNELDF
QEHMIVPHGFESFSEALRAGVETYHVLGELLEEEYGPIATNVGDEGGYAPPMKDTVEPLDVLVEAIEEAGYAPGKEIALA
LDAAASEFYDEDSGTYRAYGQKYTRDELIDVYKDLVSQYPIVSIEDPLHEEDFRGFAKITEELGDKVQIVGDDLFVTNPD
RLRKGIEMGAANALLLKVNQIGTLTEAVEAGELALQHGYGVMVSHRSGDTEDPFIADLAVALGCGQIKTGAPARSSRTAK
YNRLLRIEEDLAGAAEFGPRNDFFLP

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the archaeal cel

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI4503571, Length=430, Percent_Identity=53.7209302325581, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI5803011, Length=430, Percent_Identity=54.4186046511628, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI301897477, Length=424, Percent_Identity=54.0094339622642, Blast_Score=417, Evalue=1e-116,
Organism=Homo sapiens, GI301897469, Length=424, Percent_Identity=54.0094339622642, Blast_Score=417, Evalue=1e-116,
Organism=Homo sapiens, GI301897479, Length=422, Percent_Identity=49.5260663507109, Blast_Score=360, Evalue=1e-99,
Organism=Homo sapiens, GI169201331, Length=350, Percent_Identity=28.2857142857143, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI169201757, Length=350, Percent_Identity=28.2857142857143, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI239744207, Length=350, Percent_Identity=28.2857142857143, Blast_Score=123, Evalue=3e-28,
Organism=Escherichia coli, GI1789141, Length=430, Percent_Identity=53.7209302325581, Blast_Score=417, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI71995829, Length=434, Percent_Identity=53.6866359447005, Blast_Score=420, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17536383, Length=434, Percent_Identity=53.6866359447005, Blast_Score=420, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI32563855, Length=192, Percent_Identity=48.4375, Blast_Score=184, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6321693, Length=433, Percent_Identity=53.3487297921478, Blast_Score=402, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6324974, Length=425, Percent_Identity=52.2352941176471, Blast_Score=397, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6324969, Length=425, Percent_Identity=52.2352941176471, Blast_Score=397, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6323985, Length=425, Percent_Identity=52, Blast_Score=396, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6321968, Length=433, Percent_Identity=52.1939953810624, Blast_Score=370, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24580918, Length=431, Percent_Identity=51.0440835266821, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580916, Length=431, Percent_Identity=51.0440835266821, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580920, Length=431, Percent_Identity=51.0440835266821, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580914, Length=431, Percent_Identity=51.0440835266821, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI281360527, Length=431, Percent_Identity=51.0440835266821, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI17137654, Length=431, Percent_Identity=51.0440835266821, Blast_Score=389, Evalue=1e-108,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_METKA (Q8TUV6)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614930.1
- ProteinModelPortal:   Q8TUV6
- SMR:   Q8TUV6
- GeneID:   1478242
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK1647
- NMPDR:   fig|190192.1.peg.1643
- HOGENOM:   HBG726599
- OMA:   HAGNELD
- BioCyc:   MKAN190192:MK1647-MONOMER
- BRENDA:   4.2.1.11
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 46163; Mature: 46032

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 206-206 ACT_SITE 338-338 BINDING 154-154 BINDING 163-163 BINDING 286-286 BINDING 313-313 BINDING 338-338 BINDING 389-389

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRIVSVKAREVLDSRGEPTVEVEVELEDGTVGRAMVPSGASTGTYEALELRDGDDRYGG
CCCEECHHHHHHHHCCCCCEEEEEEEECCCCCCCEECCCCCCCCCEEEEEECCCCCCCCC
KGVRRAVRNVEEIIAPEIEGLDATAQPDIDRTMIELDGTENKSHLGANAILGVSLAVARA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHH
AAKSLGIPLYRYLGGPTARRLPVPFMNVINGGEHAGNELDFQEHMIVPHGFESFSEALRA
HHHHHCCHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHCEECCCCHHHHHHHHHH
GVETYHVLGELLEEEYGPIATNVGDEGGYAPPMKDTVEPLDVLVEAIEEAGYAPGKEIAL
HHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEE
ALDAAASEFYDEDSGTYRAYGQKYTRDELIDVYKDLVSQYPIVSIEDPLHEEDFRGFAKI
EHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHH
TEELGDKVQIVGDDLFVTNPDRLRKGIEMGAANALLLKVNQIGTLTEAVEAGELALQHGY
HHHHCCEEEEEECCEEEECHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCC
GVMVSHRSGDTEDPFIADLAVALGCGQIKTGAPARSSRTAKYNRLLRIEEDLAGAAEFGP
EEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHEEEHHHHCCHHHCCC
RNDFFLP
CCCCCCC
>Mature Secondary Structure 
PRIVSVKAREVLDSRGEPTVEVEVELEDGTVGRAMVPSGASTGTYEALELRDGDDRYGG
CCEECHHHHHHHHCCCCCEEEEEEEECCCCCCCEECCCCCCCCCEEEEEECCCCCCCCC
KGVRRAVRNVEEIIAPEIEGLDATAQPDIDRTMIELDGTENKSHLGANAILGVSLAVARA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHH
AAKSLGIPLYRYLGGPTARRLPVPFMNVINGGEHAGNELDFQEHMIVPHGFESFSEALRA
HHHHHCCHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHCEECCCCHHHHHHHHHH
GVETYHVLGELLEEEYGPIATNVGDEGGYAPPMKDTVEPLDVLVEAIEEAGYAPGKEIAL
HHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEE
ALDAAASEFYDEDSGTYRAYGQKYTRDELIDVYKDLVSQYPIVSIEDPLHEEDFRGFAKI
EHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHH
TEELGDKVQIVGDDLFVTNPDRLRKGIEMGAANALLLKVNQIGTLTEAVEAGELALQHGY
HHHHCCEEEEEECCEEEECHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCC
GVMVSHRSGDTEDPFIADLAVALGCGQIKTGAPARSSRTAKYNRLLRIEEDLAGAAEFGP
EEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHEEEHHHHCCHHHCCC
RNDFFLP
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014