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The map label for this gene is dapE [H]

Identifier: 20095017

GI number: 20095017

Start: 1592624

End: 1593769

Strand: Direct

Name: dapE [H]

Synonym: MK1581

Alternate gene names: 20095017

Gene position: 1592624-1593769 (Clockwise)

Preceding gene: 20095010

Following gene: 20095020

Centisome position: 93.96

GC content: 61.43

Gene sequence:

>1146_bases
GTGTCCGGGAACCCCTCGGGGGCACTTCAGAGGGTGAACGATCTAGAGCTCGATCCGATTGGGCTCCTGAAGGATGCCGT
AGCCACTCCGAGTGTAACCGGAGAGGAACACGAGATGACTCGCCTCCTGACGGAAGTGTTAGACGAGCACGGCGTGCCTT
ACGAGGTGGACGAGATGGGCAACGTCCTCGCGGGTGACCTGAGCGGACTGGTGCTGAACGCACACCTCGACACGGTTCCT
CCGGGCGACGGGTGGGAAGTTACGGACCCCTTCGATCCCACCATCCGCAACGGGAAGCTGTACGGACGCGGTGCGGCCGA
CTGTAAGGGAGGACTGGCGGCTGCGACGGCGGCGGTGGTTCAAGGGTACTACGAGGAGATGCCGATGGGCCTGTTGGCGA
CTGTCGGGGAAGAGTCGAGTAGTGAGGAGGACAACGGGACCCTCCACGTCTGCAGGACCCGTGAGTTGGAGGCTCGAGCC
GGAATAGTGTGTGAGCCGACGGACGGTCGAGTTCACGTGGGTGATCGAGGAAGGATCACCCTTCGAGTCACGGTGCGGGG
TCGCAGTGCCCACGCCTCGACCCCGGAGATGGGAAAGAACCCTATCGAAGCCGCATCTCGCGTCGTGGAGGCGCTCTCCA
AGTTGAGGCCTACGGAGTACAGACTACCGGAAATCGGCACCGTGAGGTCGGACTTAACGGTCACCAGAATCGAAGCGGAC
GGGCCTTCGAACGTTATACCGGAGCGGTGTGAGATGACCGTGGATTACAGGACGGTACCCGGAGAGAGTACGAAGGAAGT
GAAGAGGCGGGTCGAGAGGGTCGCCAAGCGGGCTGTGCCCTCGGGGTTCGAGGTGAGTGTGGGTATAGAGTCGGCTTCTC
GGGCCACAGTTGTGAACGTTGAGGCACCGGTGGTTAAGGCTGCGGTAATCGCCGCTCGTAAGGTCGGCTTACCGGGTAAG
CTAGACTTCAAACGGGGACACTGTGACATCGAGTACTTGGTGCATGAAGCCGGACTAGACGCCGTGATCCTGGGTCCCAG
TGGTGGAAACATCCACGGACCGGACGAGTGGGTCAAAGTGGAGGATGTTGTGCGGTGCGCCCGAGCATATCTGGCGTGTG
CGAACCTCCTCCCCACACCACATTAG

Upstream 100 bases:

>100_bases
CGGAACTGTTTATACGTTGCTTGAGTACACAATACGTGAACTCTAAAACTCAAATAGTGAACAAGGTTCTTCATCCCTTA
CGCGGGGCTCGGATAAGCGT

Downstream 100 bases:

>100_bases
CCGGGGGCGATCAGGTCCAGGACCAGGTCGCGGAACTCCTCCTCCATCTTGATCGGATCGGCTGCCGGCAGCCCGAACTC
GTCCTCGATTCTTTCACATA

Product: deacylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MSGNPSGALQRVNDLELDPIGLLKDAVATPSVTGEEHEMTRLLTEVLDEHGVPYEVDEMGNVLAGDLSGLVLNAHLDTVP
PGDGWEVTDPFDPTIRNGKLYGRGAADCKGGLAAATAAVVQGYYEEMPMGLLATVGEESSSEEDNGTLHVCRTRELEARA
GIVCEPTDGRVHVGDRGRITLRVTVRGRSAHASTPEMGKNPIEAASRVVEALSKLRPTEYRLPEIGTVRSDLTVTRIEAD
GPSNVIPERCEMTVDYRTVPGESTKEVKRRVERVAKRAVPSGFEVSVGIESASRATVVNVEAPVVKAAVIAARKVGLPGK
LDFKRGHCDIEYLVHEAGLDAVILGPSGGNIHGPDEWVKVEDVVRCARAYLACANLLPTPH

Sequences:

>Translated_381_residues
MSGNPSGALQRVNDLELDPIGLLKDAVATPSVTGEEHEMTRLLTEVLDEHGVPYEVDEMGNVLAGDLSGLVLNAHLDTVP
PGDGWEVTDPFDPTIRNGKLYGRGAADCKGGLAAATAAVVQGYYEEMPMGLLATVGEESSSEEDNGTLHVCRTRELEARA
GIVCEPTDGRVHVGDRGRITLRVTVRGRSAHASTPEMGKNPIEAASRVVEALSKLRPTEYRLPEIGTVRSDLTVTRIEAD
GPSNVIPERCEMTVDYRTVPGESTKEVKRRVERVAKRAVPSGFEVSVGIESASRATVVNVEAPVVKAAVIAARKVGLPGK
LDFKRGHCDIEYLVHEAGLDAVILGPSGGNIHGPDEWVKVEDVVRCARAYLACANLLPTPH
>Mature_380_residues
SGNPSGALQRVNDLELDPIGLLKDAVATPSVTGEEHEMTRLLTEVLDEHGVPYEVDEMGNVLAGDLSGLVLNAHLDTVPP
GDGWEVTDPFDPTIRNGKLYGRGAADCKGGLAAATAAVVQGYYEEMPMGLLATVGEESSSEEDNGTLHVCRTRELEARAG
IVCEPTDGRVHVGDRGRITLRVTVRGRSAHASTPEMGKNPIEAASRVVEALSKLRPTEYRLPEIGTVRSDLTVTRIEADG
PSNVIPERCEMTVDYRTVPGESTKEVKRRVERVAKRAVPSGFEVSVGIESASRATVVNVEAPVVKAAVIAARKVGLPGKL
DFKRGHCDIEYLVHEAGLDAVILGPSGGNIHGPDEWVKVEDVVRCARAYLACANLLPTPH

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790395, Length=270, Percent_Identity=30, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788816, Length=375, Percent_Identity=28, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1789236, Length=266, Percent_Identity=28.9473684210526, Blast_Score=94, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17551016, Length=318, Percent_Identity=24.8427672955975, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 40729; Mature: 40597

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGNPSGALQRVNDLELDPIGLLKDAVATPSVTGEEHEMTRLLTEVLDEHGVPYEVDEMG
CCCCCCHHHHHHCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEHHHHC
NVLAGDLSGLVLNAHLDTVPPGDGWEVTDPFDPTIRNGKLYGRGAADCKGGLAAATAAVV
CCHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHH
QGYYEEMPMGLLATVGEESSSEEDNGTLHVCRTRELEARAGIVCEPTDGRVHVGDRGRIT
HHHHHHCCHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCEEECCCCCEEEECCCCEEE
LRVTVRGRSAHASTPEMGKNPIEAASRVVEALSKLRPTEYRLPEIGTVRSDLTVTRIEAD
EEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECC
GPSNVIPERCEMTVDYRTVPGESTKEVKRRVERVAKRAVPSGFEVSVGIESASRATVVNV
CCCCCCCHHHCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEE
EAPVVKAAVIAARKVGLPGKLDFKRGHCDIEYLVHEAGLDAVILGPSGGNIHGPDEWVKV
CCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHH
EDVVRCARAYLACANLLPTPH
HHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SGNPSGALQRVNDLELDPIGLLKDAVATPSVTGEEHEMTRLLTEVLDEHGVPYEVDEMG
CCCCCHHHHHHCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEHHHHC
NVLAGDLSGLVLNAHLDTVPPGDGWEVTDPFDPTIRNGKLYGRGAADCKGGLAAATAAVV
CCHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHH
QGYYEEMPMGLLATVGEESSSEEDNGTLHVCRTRELEARAGIVCEPTDGRVHVGDRGRIT
HHHHHHCCHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCEEECCCCCEEEECCCCEEE
LRVTVRGRSAHASTPEMGKNPIEAASRVVEALSKLRPTEYRLPEIGTVRSDLTVTRIEAD
EEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECC
GPSNVIPERCEMTVDYRTVPGESTKEVKRRVERVAKRAVPSGFEVSVGIESASRATVVNV
CCCCCCCHHHCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEE
EAPVVKAAVIAARKVGLPGKLDFKRGHCDIEYLVHEAGLDAVILGPSGGNIHGPDEWVKV
CCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHH
EDVVRCARAYLACANLLPTPH
HHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA