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The map label for this gene is FwdB_2 [H]

Identifier: 20094963

GI number: 20094963

Start: 1537981

End: 1539282

Strand: Direct

Name: FwdB_2 [H]

Synonym: MK1527

Alternate gene names: 20094963

Gene position: 1537981-1539282 (Clockwise)

Preceding gene: 20094962

Following gene: 20094964

Centisome position: 90.74

GC content: 59.75

Gene sequence:

>1302_bases
GTGGCGAGGAAAGTGATCAAGGATGTGGTCTGTCCGTTCTGTGGTACGCTGTGTGATGATCTAGAGGTGGTCGTGGAGGA
CGGTGAGATCGTGGAGGTACGGCATGCTTGTAGGATCGGTGCTGCGAAGTTCTTGACGGCGCAGGAGGATCATCGTCACA
CCGAGCCGATGATCAAAGAGAACGGAGAATGGAAGAAAATCGACTACGAAGACGCCGCAGAAGAGACCGCGAAGATGCTG
GTAGAAGCTAAACTACCCGTACTCTACGGTTGGTCGGCGACGCTCGTAGAGGCGCAAGAGAAGGGTGTTGAACTGGCCGA
GCTCGTCGGTGGAATCATCGACAACACGGCTTCGGTGTGACACGGACCTTCGGTACTGGGACTGCAGGACGTCGGTGTAC
CCAGCTGCACGCTAGGTGAGGTGAAGAACAGGGCGGACACGGTGATCTACTGGGGCAGTAACCCGATGCACGCTCACCCG
CGGCACATGAGCCGTTACACCGCCTTCACCCGCGGCTTCTTCCGACCGAAGGGTCGTGAGGATCGTACTATCATCGTCGT
GGACCCGCGGAAGACCGCGACGGCGCGTCTAGCCGACGTGTACATCCGCGTCCGCCCTAACGAGGACTATGAGCTGATCA
GCGCGCTCCGGGCGGCTGTTCACGGGATCGAGATCGAGCGCGAGGAGGTCGCCGGAGTGCCGACGGAGCAGATCTACGAG
GTCGCTGACCTGATCAAGGAGGCCAGCTTCGGCACACTGTTCTGGGCGATGGGTCTAACGATGTCCCGGGGTCGTCATCG
GAACATCGACAACGCGATCTGTCTGATCAAGGACCTGAACGAGTACACGAAGTGGACGCTGATCATGATGCGAGGCCACT
ACAACGTGACCGGGTTCAACGAGGTACTGGCGTGGACGACCGGATACCCGTACGCCGTGGACTTCAGCCGTGGGTACCCG
CGGTACAACCCGGGTGAGACGAGCACCGTGGACCTACTAACCCGCGGCGAGGTCGACGCCATGATGGTCATTGCCAGTGA
TCCGGGTGCGCACTTCCCGCGGAAGGCTGTGGAGCACATGGCTAGGATCCCGCTCGTGTGCGTGGACCCGCACTGGACGC
CGACCGCCGAACTCGCTGACCTGTACGTGCCCGTAACCATCGCCGGCATCGAATGGGAAGGCACCGCCTACCGAATGGAC
TCAGTACCCATCAGGATGCGCAAGGTCGTCGAACCACCCGAAAGCATGCTCAACGACGTAGAATTCCTCGAAATGGTCAT
AGAAAAGGTCGAGGAGATGTAA

Upstream 100 bases:

>100_bases
AGTATCTCTCCGAATAACCTCATGGTAGCTTGGTGGTTCACGTTTACCCATCATTTTCATCGAATATCGACATCGCTAAC
AACGGGAAGGGGGTGAAAAA

Downstream 100 bases:

>100_bases
GCCCGGGCCGCCCCACGGGGCGAACCCCGTCCCCGGGGAGCCCCGTGGGGTGAAGCGGGGGCAAGTCCCCCGCGGCCCGG
GCCTTTCACTCCGTAACTCC

Product: formylmethanofuran dehydrogenase subunit B, selenocysteine containing

Products: NA

Alternate protein names: Tungsten-containing formylmethanofuran dehydrogenase II subunit B [H]

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MARKVIKDVVCPFCGTLCDDLEVVVEDGEIVEVRHACRIGAAKFLTAQEDHRHTEPMIKENGEWKKIDYEDAAEETAKML
VEAKLPVLYGWSATLVEAQEKGVELAELVGGIIDNTASVUHGPSVLGLQDVGVPSCTLGEVKNRADTVIYWGSNPMHAHP
RHMSRYTAFTRGFFRPKGREDRTIIVVDPRKTATARLADVYIRVRPNEDYELISALRAAVHGIEIEREEVAGVPTEQIYE
VADLIKEASFGTLFWAMGLTMSRGRHRNIDNAICLIKDLNEYTKWTLIMMRGHYNVTGFNEVLAWTTGYPYAVDFSRGYP
RYNPGETSTVDLLTRGEVDAMMVIASDPGAHFPRKAVEHMARIPLVCVDPHWTPTAELADLYVPVTIAGIEWEGTAYRMD
SVPIRMRKVVEPPESMLNDVEFLEMVIEKVEEM

Sequences:

>Translated_433_residues
MARKVIKDVVCPFCGTLCDDLEVVVEDGEIVEVRHACRIGAAKFLTAQEDHRHTEPMIKENGEWKKIDYEDAAEETAKML
VEAKLPVLYGWSATLVEAQEKGVELAELVGGIIDNTASV*HGPSVLGLQDVGVPSCTLGEVKNRADTVIYWGSNPMHAHP
RHMSRYTAFTRGFFRPKGREDRTIIVVDPRKTATARLADVYIRVRPNEDYELISALRAAVHGIEIEREEVAGVPTEQIYE
VADLIKEASFGTLFWAMGLTMSRGRHRNIDNAICLIKDLNEYTKWTLIMMRGHYNVTGFNEVLAWTTGYPYAVDFSRGYP
RYNPGETSTVDLLTRGEVDAMMVIASDPGAHFPRKAVEHMARIPLVCVDPHWTPTAELADLYVPVTIAGIEWEGTAYRMD
SVPIRMRKVVEPPESMLNDVEFLEMVIEKVEEM
>Mature_432_residues
ARKVIKDVVCPFCGTLCDDLEVVVEDGEIVEVRHACRIGAAKFLTAQEDHRHTEPMIKENGEWKKIDYEDAAEETAKMLV
EAKLPVLYGWSATLVEAQEKGVELAELVGGIIDNTASV*HGPSVLGLQDVGVPSCTLGEVKNRADTVIYWGSNPMHAHPR
HMSRYTAFTRGFFRPKGREDRTIIVVDPRKTATARLADVYIRVRPNEDYELISALRAAVHGIEIEREEVAGVPTEQIYEV
ADLIKEASFGTLFWAMGLTMSRGRHRNIDNAICLIKDLNEYTKWTLIMMRGHYNVTGFNEVLAWTTGYPYAVDFSRGYPR
YNPGETSTVDLLTRGEVDAMMVIASDPGAHFPRKAVEHMARIPLVCVDPHWTPTAELADLYVPVTIAGIEWEGTAYRMDS
VPIRMRKVVEPPESMLNDVEFLEMVIEKVEEM

Specific function: Catalyzes the reversible oxidation of CO(2) and methanofuran (MFR) to N-formylmethanofuran (CHO-MFR). This enzyme is oxygen-labile [H]

COG id: COG1029

COG function: function code C; Formylmethanofuran dehydrogenase subunit B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fwdB family [H]

Homologues:

Organism=Escherichia coli, GI3868721, Length=506, Percent_Identity=25.4940711462451, Blast_Score=85, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016457
- InterPro:   IPR006656 [H]

Pfam domain/function: PF00384 Molybdopterin [H]

EC number: =1.2.99.5 [H]

Molecular weight: Translated: 48535; Mature: 48404

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKVIKDVVCPFCGTLCDDLEVVVEDGEIVEVRHACRIGAAKFLTAQEDHRHTEPMIKE
CCHHHHHHHHHHHHHHHHCCCEEEECCCCEEEHHHHHHHCHHHHHCCCHHCCCCCCHHHC
NGEWKKIDYEDAAEETAKMLVEAKLPVLYGWSATLVEAQEKGVELAELVGGIIDNTASVH
CCCEEECCHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHCCCHHHHHHHHHHCCCCCCC
GPSVLGLQDVGVPSCTLGEVKNRADTVIYWGSNPMHAHPRHMSRYTAFTRGFFRPKGRED
CCEEECCCCCCCCCCCHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
RTIIVVDPRKTATARLADVYIRVRPNEDYELISALRAAVHGIEIEREEVAGVPTEQIYEV
CEEEEECCCCCHHHEEEEEEEEECCCCCHHHHHHHHHHHHCCEEEHHHHCCCCHHHHHHH
ADLIKEASFGTLFWAMGLTMSRGRHRNIDNAICLIKDLNEYTKWTLIMMRGHYNVTGFNE
HHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHCEEEEEEEECCCCCCCHHH
VLAWTTGYPYAVDFSRGYPRYNPGETSTVDLLTRGEVDAMMVIASDPGAHFPRKAVEHMA
HHEEECCCCEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCHHHHHHHH
RIPLVCVDPHWTPTAELADLYVPVTIAGIEWEGTAYRMDSVPIRMRKVVEPPESMLNDVE
HCCEEEECCCCCCCHHHHHEEEEEEEEEEEECCCEEEECCCCHHHHHHCCCHHHHHHHHH
FLEMVIEKVEEM
HHHHHHHHHHCC
>Mature Secondary Structure 
ARKVIKDVVCPFCGTLCDDLEVVVEDGEIVEVRHACRIGAAKFLTAQEDHRHTEPMIKE
CHHHHHHHHHHHHHHHHCCCEEEECCCCEEEHHHHHHHCHHHHHCCCHHCCCCCCHHHC
NGEWKKIDYEDAAEETAKMLVEAKLPVLYGWSATLVEAQEKGVELAELVGGIIDNTASVH
CCCEEECCHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHCCCHHHHHHHHHHCCCCCCC
GPSVLGLQDVGVPSCTLGEVKNRADTVIYWGSNPMHAHPRHMSRYTAFTRGFFRPKGRED
CCEEECCCCCCCCCCCHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
RTIIVVDPRKTATARLADVYIRVRPNEDYELISALRAAVHGIEIEREEVAGVPTEQIYEV
CEEEEECCCCCHHHEEEEEEEEECCCCCHHHHHHHHHHHHCCEEEHHHHCCCCHHHHHHH
ADLIKEASFGTLFWAMGLTMSRGRHRNIDNAICLIKDLNEYTKWTLIMMRGHYNVTGFNE
HHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHCEEEEEEEECCCCCCCHHH
VLAWTTGYPYAVDFSRGYPRYNPGETSTVDLLTRGEVDAMMVIASDPGAHFPRKAVEHMA
HHEEECCCCEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCHHHHHHHH
RIPLVCVDPHWTPTAELADLYVPVTIAGIEWEGTAYRMDSVPIRMRKVVEPPESMLNDVE
HCCEEEECCCCCCCHHHHHEEEEEEEEEEEECCCEEEECCCCHHHHHHCCCHHHHHHHHH
FLEMVIEKVEEM
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]