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The map label for this gene is HisH

Identifier: 20094946

GI number: 20094946

Start: 1526044

End: 1526646

Strand: Direct

Name: HisH

Synonym: MK1510

Alternate gene names: 20094946

Gene position: 1526044-1526646 (Clockwise)

Preceding gene: 20094944

Following gene: 20094947

Centisome position: 90.03

GC content: 58.21

Gene sequence:

>603_bases
TTGAAAGTGGCGATAGTAGAGTACGGCGTCGGCAACTTGAACAGCGTGTACCGCGCGGTTAAACACTTGGGGCATGAGCC
CGTCGTTACTGATGATCCCTCGGAGTTGCACGACGCCGACGCCGTGATTCTCCCAGGTGTGGGAAACTTCCGCGCCGCCG
CGGAGAAACTCGAAGAGACCGGATTATGTGACGAGATCCGAGAGCTGCTAGGGTCCGTCCCACTCTTAGGTATATGCCTT
GGGATGCAGCTCTTGATGGAATCGAGCGAGGAGAGCCCCGAGTCACGCGGTCTAGGAGTGTTTCGGGGAACGTGTGTGGC
GCTCCCCAATGACGTGAAAACGCCGCACATGGGATGGAACACGGTGGAGTTCCGGACGGAAGAGTTTCGGGAGTTCGACG
GTGAGATGTTCTACTTCGTCCACTCGTACCGGGTGGCTCCCGAGGACGACGTCGTCCTAGGTGAGACGGAGTACGGGGAA
CGGTTTCCAAGCGTGATCGGGGACCGGGGTAGACTGATCTACGGAATGCAATTCCACCCCGAAAAAAGCGGACCAGTGGG
GTTAAAGTTGCTCGGTGAGGTTCTTACGGGGGCGAGCCGTTGA

Upstream 100 bases:

>100_bases
GCCGACACTCAGCCCGTGCTCCCGTAGTAACTCCCCGGCCTTACCTTTGTACTCGGACAACACCCTCACCCCAGACCCAT
CCTTCCAAGGGGGACTCGGC

Downstream 100 bases:

>100_bases
GAATGTCCCGCGAGAGAATATTCCGAGAAGTTAAAGAATCCCTGAAGAACATCCAAGAGGTGAGATCCGAGCTAGGTGAC
AATGTGTCGCTGTCGACCCT

Product: glutamine amidotransferase

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MKVAIVEYGVGNLNSVYRAVKHLGHEPVVTDDPSELHDADAVILPGVGNFRAAAEKLEETGLCDEIRELLGSVPLLGICL
GMQLLMESSEESPESRGLGVFRGTCVALPNDVKTPHMGWNTVEFRTEEFREFDGEMFYFVHSYRVAPEDDVVLGETEYGE
RFPSVIGDRGRLIYGMQFHPEKSGPVGLKLLGEVLTGASR

Sequences:

>Translated_200_residues
MKVAIVEYGVGNLNSVYRAVKHLGHEPVVTDDPSELHDADAVILPGVGNFRAAAEKLEETGLCDEIRELLGSVPLLGICL
GMQLLMESSEESPESRGLGVFRGTCVALPNDVKTPHMGWNTVEFRTEEFREFDGEMFYFVHSYRVAPEDDVVLGETEYGE
RFPSVIGDRGRLIYGMQFHPEKSGPVGLKLLGEVLTGASR
>Mature_200_residues
MKVAIVEYGVGNLNSVYRAVKHLGHEPVVTDDPSELHDADAVILPGVGNFRAAAEKLEETGLCDEIRELLGSVPLLGICL
GMQLLMESSEESPESRGLGVFRGTCVALPNDVKTPHMGWNTVEFRTEEFREFDGEMFYFVHSYRVAPEDDVVLGETEYGE
RFPSVIGDRGRLIYGMQFHPEKSGPVGLKLLGEVLTGASR

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=204, Percent_Identity=42.156862745098, Blast_Score=130, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6319725, Length=212, Percent_Identity=32.0754716981132, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_METKA (Q8TV83)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614793.1
- ProteinModelPortal:   Q8TV83
- SMR:   Q8TV83
- GeneID:   1478105
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK1510
- NMPDR:   fig|190192.1.peg.1506
- HOGENOM:   HBG292341
- OMA:   VCVLKEV
- BioCyc:   MKAN190192:MK1510-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 22013; Mature: 22013

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 79-79 ACT_SITE 179-179 ACT_SITE 181-181

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAIVEYGVGNLNSVYRAVKHLGHEPVVTDDPSELHDADAVILPGVGNFRAAAEKLEET
CEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCHHHCCCCEEEECCCCCHHHHHHHHHHC
GLCDEIRELLGSVPLLGICLGMQLLMESSEESPESRGLGVFRGTCVALPNDVKTPHMGWN
CCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHCCCEEECCEEEECCCCCCCCCCCCC
TVEFRTEEFREFDGEMFYFVHSYRVAPEDDVVLGETEYGERFPSVIGDRGRLIYGMQFHP
CHHHHHHHHHHCCCCEEEEEEEEECCCCCCEEECCCHHHHHHHHHHCCCCCEEEEEEECC
EKSGPVGLKLLGEVLTGASR
CCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKVAIVEYGVGNLNSVYRAVKHLGHEPVVTDDPSELHDADAVILPGVGNFRAAAEKLEET
CEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCHHHCCCCEEEECCCCCHHHHHHHHHHC
GLCDEIRELLGSVPLLGICLGMQLLMESSEESPESRGLGVFRGTCVALPNDVKTPHMGWN
CCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHCCCEEECCEEEECCCCCCCCCCCCC
TVEFRTEEFREFDGEMFYFVHSYRVAPEDDVVLGETEYGERFPSVIGDRGRLIYGMQFHP
CHHHHHHHHHHCCCCEEEEEEEEECCCCCCEEECCCHHHHHHHHHHCCCCCEEEEEEECC
EKSGPVGLKLLGEVLTGASR
CCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014