Click here to switch to the map view.

The map label for this gene is NadE

Identifier: 20094854

GI number: 20094854

Start: 1446749

End: 1447603

Strand: Direct

Name: NadE

Synonym: MK1418

Alternate gene names: 20094854

Gene position: 1446749-1447603 (Clockwise)

Preceding gene: 20094853

Following gene: 20094855

Centisome position: 85.36

GC content: 63.63

Gene sequence:

>855_bases
ATGGCAAAGTACGAACCGGTCATACCGGAGCTCGAGGTGAATCCTGAGGGGGAAGTGAGCAAGATAGCGGAGTTCCTCCG
GGGGAAGTTCGAGGAGGCCGGTCGGGAGATCGCGGTAGTGGGTTTGAGCGGCGGAGTCGACTCATCTACGACCCTCGGAC
TCGCGGTCGAGGCGCTAGGCCGGGAGAACGTGGTAGCGCTGATACTACCGGAGCGTGACACCCCAGAGGAGGACGTTGAA
GACGCCGTGGAAGCCGCGGAACGGTTCGGTGTCGAGTACCACGTCCACGACATCACCGAGGTCCTGCGAGCCTTCGGCAC
CGGTTCATATGTCCCCTGTCACCCGTTCAGCCGCAAGAGCGACGCTAACCTCAAACCGCGGGTCCGTATGTGCGTACTGT
ATTATTTCGCGAACGAGCTCGACGGTCTCGTCCTGGGTACCGGCAACCGTACGGAGTGGCTCACGGGGTACTTCACCCTG
CACGGAGACGGCGCCTGCGACGTGGCCCCGATCAGGCACCTTTACAAGACGCAGGTTTACGTGATAGCCGAGCATCTCGG
CGTGCCGGAGCGCATCGTGGAGGAGAAGGAACCCTCGGCGCGGCTCTGGCCCGGCCAGACCGATGAGGGTGAACTCGGGA
TCGATTACCCCACGCTCGATGCCCTCCTGTACGCGCTCGTCGACGAGGGTCTAGGGCCTCGGAAGGCCGTCGATTGGCTC
GGGGAGCGGGGCGTAGAGGCGACGGAGGAGGACGCCGAAAAGGTCCTGGACCTTGTGCGAAGTTCCTCGTTCAAGCGACG
GCCGGCTCCTGGTCTGGACTTGCCCGAGCCCGAGGATCCTGCGATGTCGGGGTGA

Upstream 100 bases:

>100_bases
CACCTTTAGGGCTTCGCAAGGACAACGTGAGCTCACCAGGAAGAAACAAAGAAAAAAGTGTTTAAGAGACAGTTTTGGAG
TGAATCCCCAGGGGTAACCG

Downstream 100 bases:

>100_bases
AGAGGGAGTTCGAGCCGTATGGAGAAGGTAGTCCTAGAGTTCGGATGTGAGTTCGGCGCGCGCCTATGGGTAATCCAGCA
GGTGATCTGCATCCTCGTTT

Product: NAD synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MAKYEPVIPELEVNPEGEVSKIAEFLRGKFEEAGREIAVVGLSGGVDSSTTLGLAVEALGRENVVALILPERDTPEEDVE
DAVEAAERFGVEYHVHDITEVLRAFGTGSYVPCHPFSRKSDANLKPRVRMCVLYYFANELDGLVLGTGNRTEWLTGYFTL
HGDGACDVAPIRHLYKTQVYVIAEHLGVPERIVEEKEPSARLWPGQTDEGELGIDYPTLDALLYALVDEGLGPRKAVDWL
GERGVEATEEDAEKVLDLVRSSSFKRRPAPGLDLPEPEDPAMSG

Sequences:

>Translated_284_residues
MAKYEPVIPELEVNPEGEVSKIAEFLRGKFEEAGREIAVVGLSGGVDSSTTLGLAVEALGRENVVALILPERDTPEEDVE
DAVEAAERFGVEYHVHDITEVLRAFGTGSYVPCHPFSRKSDANLKPRVRMCVLYYFANELDGLVLGTGNRTEWLTGYFTL
HGDGACDVAPIRHLYKTQVYVIAEHLGVPERIVEEKEPSARLWPGQTDEGELGIDYPTLDALLYALVDEGLGPRKAVDWL
GERGVEATEEDAEKVLDLVRSSSFKRRPAPGLDLPEPEDPAMSG
>Mature_283_residues
AKYEPVIPELEVNPEGEVSKIAEFLRGKFEEAGREIAVVGLSGGVDSSTTLGLAVEALGRENVVALILPERDTPEEDVED
AVEAAERFGVEYHVHDITEVLRAFGTGSYVPCHPFSRKSDANLKPRVRMCVLYYFANELDGLVLGTGNRTEWLTGYFTLH
GDGACDVAPIRHLYKTQVYVIAEHLGVPERIVEEKEPSARLWPGQTDEGELGIDYPTLDALLYALVDEGLGPRKAVDWLG
ERGVEATEEDAEKVLDLVRSSSFKRRPAPGLDLPEPEDPAMSG

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family

Homologues:

Organism=Escherichia coli, GI1788036, Length=231, Percent_Identity=29.4372294372294, Blast_Score=71, Evalue=7e-14,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NADE_METKA (Q8TVH1)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614701.1
- ProteinModelPortal:   Q8TVH1
- SMR:   Q8TVH1
- GeneID:   1478013
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK1418
- NMPDR:   fig|190192.1.peg.1414
- HOGENOM:   HBG351567
- OMA:   QIASAYS
- BioCyc:   MKAN190192:MK1418-MONOMER
- BRENDA:   6.3.1.5
- HAMAP:   MF_00193
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00552

Pfam domain/function: PF02540 NAD_synthase

EC number: =6.3.1.5

Molecular weight: Translated: 31191; Mature: 31060

Theoretical pI: Translated: 4.27; Mature: 4.27

Prosite motif: NA

Important sites: ACT_SITE 43-43

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKYEPVIPELEVNPEGEVSKIAEFLRGKFEEAGREIAVVGLSGGVDSSTTLGLAVEALG
CCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHCC
RENVVALILPERDTPEEDVEDAVEAAERFGVEYHVHDITEVLRAFGTGSYVPCHPFSRKS
CCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCC
DANLKPRVRMCVLYYFANELDGLVLGTGNRTEWLTGYFTLHGDGACDVAPIRHLYKTQVY
CCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHH
VIAEHLGVPERIVEEKEPSARLWPGQTDEGELGIDYPTLDALLYALVDEGLGPRKAVDWL
HHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHH
GERGVEATEEDAEKVLDLVRSSSFKRRPAPGLDLPEPEDPAMSG
HHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AKYEPVIPELEVNPEGEVSKIAEFLRGKFEEAGREIAVVGLSGGVDSSTTLGLAVEALG
CCCCCCCCCEECCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHCC
RENVVALILPERDTPEEDVEDAVEAAERFGVEYHVHDITEVLRAFGTGSYVPCHPFSRKS
CCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCC
DANLKPRVRMCVLYYFANELDGLVLGTGNRTEWLTGYFTLHGDGACDVAPIRHLYKTQVY
CCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHH
VIAEHLGVPERIVEEKEPSARLWPGQTDEGELGIDYPTLDALLYALVDEGLGPRKAVDWL
HHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHH
GERGVEATEEDAEKVLDLVRSSSFKRRPAPGLDLPEPEDPAMSG
HHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014