Click here to switch to the map view.

The map label for this gene is frhA [H]

Identifier: 20094367

GI number: 20094367

Start: 889351

End: 890601

Strand: Reverse

Name: frhA [H]

Synonym: MK0931

Alternate gene names: 20094367

Gene position: 890601-889351 (Counterclockwise)

Preceding gene: 20094369

Following gene: 20094366

Centisome position: 52.54

GC content: 59.95

Gene sequence:

>1251_bases
TTGGCGGAAGGTGCCGTGGAGATTCAACCGACCACGAGACATGAAGGTCACGCTAAGCTAGTATTATATGTCGACGACGA
GGGTTACGTGGAGCGTGCGTTTTACCTGAACACTTCGGCGGTCCGAGGCTTCGAAGCGCTGGCGAAGGGAAGGCCCGCGG
AGTTCGTTCAGGTGGCCGTTATGAGGATCTGCGGAATCTGCCAGGCGACGCACGGAACCGCATCCGCCGAGGCCTTCGAG
CGGGCCATGGGCATCGAACCTCCCAAGGACGGGAAACTCCTGCGGGAACTCTGCGCCCTGGGTAACAGGATTCAGTCTCA
CGTGCTCCATCAGTTGCTAGTACTGGACGATTTCGTTGAGGATGAGAGCGAGAAGGTCGAGGCGGTCAAGAGGATCCAGC
AGATCAGGCGGATCGGACAGTACGTGGTGGACGTGGTCGGAGGTGAGGGAATCCATCCACCCAACATCAGGATCGGAGGG
ATGGCCGAGAACATCTCGGAAGCCGCGCGTAGGAAGCTTTACCGCAGGCTACGTGAGGCGCGCGAGCTGATGATGGAGCA
GCACGAGTTCATGGTGAACATCGTCGAGAGGTTCGGCGACGAGAACGACCTCGACATCGACGAGTTCGGCAGACACGACC
AGCCGTTCCTGGCGACGCATCCCACCTATGGGGATCCCGACCGGCTGGACATGGACCGCGTGGTGGAGCTGCTTCCGATC
GAGTACTACGGAGAGGAGCACAAGGAAGTCGCGTACCAGCACCGGGGCCAGATCCCGCTTTACGACGGTGTTCCGGTGGA
AGTCGGACCCAGAGCCAGGTACATACTCTTCGACGGGGTGGACCCACGCGGAGTCCTGTACATCCACGTGTTAAGGTCAC
AGGAAACACTCGCGGCCATCGACCGGGCGATGACGATCCTGGATGAACTCAACACCAGCGGCAAGACCCTGGCCGAGTGG
GAGCCGAAGGCAGGAGTCGGAATCGGAGTTCACGAGGCCCCACGGGGCACCAACGTGCACATCGCTAAGGTGAACGAGAA
GGGTATCGTCGAGGACTATCGCATCATCGCGGCTTCCACGTGGAACTTCCCGGTGGTGGAGAAGGCTATCGAGGGCGAAA
ACGAAGAGTACGCCGAGGTCATCATGCGATGTTACGACATATGAGCATCGTGTGCCGCTCACGTGGTGAAGGAAGTCCGC
GACGCCGACTCCCGGGAGAAGATCCGGGAGTCGGTAGTTAAGCTCGCCTAA

Upstream 100 bases:

>100_bases
GTATTCAACCGTTAAATGTGCCTCTACTCGATCACTGAACACACCCGAGTTTTATGTTCTTCTTCCGTGGCCGTCGGGAC
GGGCCGATGGGGGTGAGGCG

Downstream 100 bases:

>100_bases
AACCGACTATCGGGATGACCGCCGCGGGGAACCCCGAAACGGGGACCCGCGGCGCGACCGCCCCCCACGTGGGGGGCCGG
TTTCCTCACCTCACCCTTGG

Product: coenzyme F420-reducing hydrogenase, alpha subunit

Products: NA

Alternate protein names: 8-hydroxy-5-deazaflavin-reducing hydrogenase subunit alpha; FRH [H]

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MAEGAVEIQPTTRHEGHAKLVLYVDDEGYVERAFYLNTSAVRGFEALAKGRPAEFVQVAVMRICGICQATHGTASAEAFE
RAMGIEPPKDGKLLRELCALGNRIQSHVLHQLLVLDDFVEDESEKVEAVKRIQQIRRIGQYVVDVVGGEGIHPPNIRIGG
MAENISEAARRKLYRRLREARELMMEQHEFMVNIVERFGDENDLDIDEFGRHDQPFLATHPTYGDPDRLDMDRVVELLPI
EYYGEEHKEVAYQHRGQIPLYDGVPVEVGPRARYILFDGVDPRGVLYIHVLRSQETLAAIDRAMTILDELNTSGKTLAEW
EPKAGVGIGVHEAPRGTNVHIAKVNEKGIVEDYRIIAASTWNFPVVEKAIEGENEEYAEVIMRCYDIUASCAAHVVKEVR
DADSREKIRESVVKLA

Sequences:

>Translated_416_residues
MAEGAVEIQPTTRHEGHAKLVLYVDDEGYVERAFYLNTSAVRGFEALAKGRPAEFVQVAVMRICGICQATHGTASAEAFE
RAMGIEPPKDGKLLRELCALGNRIQSHVLHQLLVLDDFVEDESEKVEAVKRIQQIRRIGQYVVDVVGGEGIHPPNIRIGG
MAENISEAARRKLYRRLREARELMMEQHEFMVNIVERFGDENDLDIDEFGRHDQPFLATHPTYGDPDRLDMDRVVELLPI
EYYGEEHKEVAYQHRGQIPLYDGVPVEVGPRARYILFDGVDPRGVLYIHVLRSQETLAAIDRAMTILDELNTSGKTLAEW
EPKAGVGIGVHEAPRGTNVHIAKVNEKGIVEDYRIIAASTWNFPVVEKAIEGENEEYAEVIMRCYDI*ASCAAHVVKEVR
DADSREKIRESVVKLA
>Mature_415_residues
AEGAVEIQPTTRHEGHAKLVLYVDDEGYVERAFYLNTSAVRGFEALAKGRPAEFVQVAVMRICGICQATHGTASAEAFER
AMGIEPPKDGKLLRELCALGNRIQSHVLHQLLVLDDFVEDESEKVEAVKRIQQIRRIGQYVVDVVGGEGIHPPNIRIGGM
AENISEAARRKLYRRLREARELMMEQHEFMVNIVERFGDENDLDIDEFGRHDQPFLATHPTYGDPDRLDMDRVVELLPIE
YYGEEHKEVAYQHRGQIPLYDGVPVEVGPRARYILFDGVDPRGVLYIHVLRSQETLAAIDRAMTILDELNTSGKTLAEWE
PKAGVGIGVHEAPRGTNVHIAKVNEKGIVEDYRIIAASTWNFPVVEKAIEGENEEYAEVIMRCYDI*ASCAAHVVKEVRD
ADSREKIRESVVKLA

Specific function: Reduces the physiological low-potential two-electron acceptor coenzyme F420, and the artificial one-electron acceptor methylviologen [H]

COG id: COG3259

COG function: function code C; Coenzyme F420-reducing hydrogenase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789368, Length=138, Percent_Identity=27.536231884058, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787207, Length=111, Percent_Identity=31.5315315315315, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017682
- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.98.1 [H]

Molecular weight: Translated: 46701; Mature: 46569

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00507 NI_HGENASE_L_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEGAVEIQPTTRHEGHAKLVLYVDDEGYVERAFYLNTSAVRGFEALAKGRPAEFVQVAV
CCCCCEEECCCCCCCCCEEEEEEECCCCCEEHEEEECHHHHHHHHHHHCCCCHHHHHHHH
MRICGICQATHGTASAEAFERAMGIEPPKDGKLLRELCALGNRIQSHVLHQLLVLDDFVE
HHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DESEKVEAVKRIQQIRRIGQYVVDVVGGEGIHPPNIRIGGMAENISEAARRKLYRRLREA
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHH
RELMMEQHEFMVNIVERFGDENDLDIDEFGRHDQPFLATHPTYGDPDRLDMDRVVELLPI
HHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHH
EYYGEEHKEVAYQHRGQIPLYDGVPVEVGPRARYILFDGVDPRGVLYIHVLRSQETLAAI
HHCCCHHHHHHHHHCCCCCCCCCCCCEECCCEEEEEEECCCCCCEEEEEEECCCHHHHHH
DRAMTILDELNTSGKTLAEWEPKAGVGIGVHEAPRGTNVHIAKVNEKGIVEDYRIIAAST
HHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEECC
WNFPVVEKAIEGENEEYAEVIMRCYDIASCAAHVVKEVRDADSREKIRESVVKLA
CCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
AEGAVEIQPTTRHEGHAKLVLYVDDEGYVERAFYLNTSAVRGFEALAKGRPAEFVQVAV
CCCCEEECCCCCCCCCEEEEEEECCCCCEEHEEEECHHHHHHHHHHHCCCCHHHHHHHH
MRICGICQATHGTASAEAFERAMGIEPPKDGKLLRELCALGNRIQSHVLHQLLVLDDFVE
HHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DESEKVEAVKRIQQIRRIGQYVVDVVGGEGIHPPNIRIGGMAENISEAARRKLYRRLREA
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHH
RELMMEQHEFMVNIVERFGDENDLDIDEFGRHDQPFLATHPTYGDPDRLDMDRVVELLPI
HHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHH
EYYGEEHKEVAYQHRGQIPLYDGVPVEVGPRARYILFDGVDPRGVLYIHVLRSQETLAAI
HHCCCHHHHHHHHHCCCCCCCCCCCCEECCCEEEEEEECCCCCCEEEEEEECCCHHHHHH
DRAMTILDELNTSGKTLAEWEPKAGVGIGVHEAPRGTNVHIAKVNEKGIVEDYRIIAAST
HHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEECC
WNFPVVEKAIEGENEEYAEVIMRCYDIASCAAHVVKEVRDADSREKIRESVVKLA
CCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]