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The map label for this gene is CarB_2

Identifier: 20094347

GI number: 20094347

Start: 866594

End: 868288

Strand: Reverse

Name: CarB_2

Synonym: MK0911

Alternate gene names: 20094347

Gene position: 868288-866594 (Counterclockwise)

Preceding gene: 20094350

Following gene: 20094346

Centisome position: 51.23

GC content: 62.24

Gene sequence:

>1695_bases
GTGCCCGAGACTCCGAATAAGGTCCTGATCATCGGATCCGGACCGATCATCGTGGGTCAAGCGGCAGAGTTCGACTACTC
GGGTTCTCAGGCCTGCAAGGCGCTCAGAGAAGAGGGTGTTGAAGTAGTTCTCGTGAACAGTAACCCGGCGACGATCATGA
CGGATCCGAACATGGCGGATCGAGTGTATCTGGAGCCGTTGGACGCCCGGATCGTAGCGAAGATCATCGAGGAGGAGAGA
CCCGACGGAATCCTCCCCACGCTCGGGGGTCAGACCGGTCTCAATATCGCGGTCGAGCTCGACGAGATGGGCGTTTTGGA
AGAGTACGACGTAGAGGTGCTGGGGACATCGGTGGAGACCATCGTACGGGCGGAGGACCGGGACGAGTTCCGTGCGTTCA
TGAAGAAGATCGGGGAACCCGTCTGCGCGAGCGAGGCGGTTTCTTCGGTGGAGGAAGCCAAGGAGGTAGCGGAGGAAATC
GGTTACCCGGTGGTGGTGCGGCCCGCCTACACGCTGGGCGGTACGGGCGGCGGAATCGCGGAGGACGAGGAGGAGCTGAA
GAGGGTAGTGGAGCGGGGCCTCGAGTACAGCCGGGTGAACCAGGTGCTGATCGAGGAGTACGTGGGTGGCTGGGCCGAGA
TCGAGTACGAGGTGATGCGGGACGGATCGGGTAACTGCATCACGGTGTGCAGCATGGAGAACGTGGATCCGATGGGGGTT
CACACCGGCGAGTCTATCGTAGTCGCGCCCGCTCAGACGCTAACCGAGGAGGAGCATCAGATGCTCCGCAGCGCGGCGCT
GCACATCATCGACGCGCTGGGTGTTGAGGGCGGTTGCAACATCCAGTTCGCGCTGCACCGGGAGACGGGCGAGTACCGGG
TGATCGAGGTGAACCCGCGTGTCAGTCGATCGTCGGCGCTGGCGTCCAAGGCCACGGGATACCCGATCGCACGGATCGCT
GCGAAGATCGCGATAGGGCTGCGACTGGACGAAATCGAGAACCAGGTCACCGGAGAGACGTACGCGGCGTTCGAGCCGGC
CCTGGACTACGTGGTGGTGAAGATCCCGCGATGGCCGTTCGACAAGTTCCCGGAGGCGAACCGGACGCTAGGAACGGAGA
TGAAGTCCGTGGGTGAGGTGATGGCCATCGGGCGGACGTTCGAAGAGGCGCTTCAGAAGGCGATTCGATCGTTGGAAATC
GGAGAGCCTGGCTTAGGGCCTTCACCGGAGGAGCTGGAGGCGGATCCGGAGGAGATCCGCCGCAAGATCGAGACGCCGAA
CGATCGCCGTATCTTCTGCATCTACGCCGCACTGAAGCGCGGTCTGATGAGCGTGGAGGAGATCTCGGAACTATCCGGTA
TCGACCCGTGGTTCGTGGAGAAGGTGAAGCGGATCGTCGAGATGGAACACGAGATCGTACGAAGGAAGGATGAGTTGCTC
GAGTTCATCCGGACCGGGGAGGCGGACGAGGAAACCGTGGAGTTCGTCCGCGAGGTCAAGCGGACGGGTTTCTCGGACGA
GCAGATAGCGGAGCTGCTCGGGGTGGACGAAGACGAGATCCGGGAGGCTCGGCTGGGTGTCGGTGTGGAGGCCACGTACA
AACTGGTGGATACGTGCGCCGCGGAGTTCGCCGCGGTCTCGCCGTACTTCTACTCGACGTACGAGGAGGAGTGCGAAGCG
CTGCGGTACGATTAG

Upstream 100 bases:

>100_bases
AGGGTCGAAAAGTTGCACTGGGGATCCGCGCGCCGGTAACGCCGAACTTGCCCTCCGTCGATTTTATTAAGCCGATCCGA
GCGGTCGGGAGCGGGTTCAC

Downstream 100 bases:

>100_bases
ACCGGGGGAACGGCATCAGGTCGTCTAAATTCGAGGCCGTGGCCGTTTCGCTCCTCGTCACGGTAGCTAGTCTTTCGCCT
GCCATCCCTGAACTTTGGGG

Product: carbamoylphosphate synthase large subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase ammonia chain

Number of amino acids: Translated: 564; Mature: 563

Protein sequence:

>564_residues
MPETPNKVLIIGSGPIIVGQAAEFDYSGSQACKALREEGVEVVLVNSNPATIMTDPNMADRVYLEPLDARIVAKIIEEER
PDGILPTLGGQTGLNIAVELDEMGVLEEYDVEVLGTSVETIVRAEDRDEFRAFMKKIGEPVCASEAVSSVEEAKEVAEEI
GYPVVVRPAYTLGGTGGGIAEDEEELKRVVERGLEYSRVNQVLIEEYVGGWAEIEYEVMRDGSGNCITVCSMENVDPMGV
HTGESIVVAPAQTLTEEEHQMLRSAALHIIDALGVEGGCNIQFALHRETGEYRVIEVNPRVSRSSALASKATGYPIARIA
AKIAIGLRLDEIENQVTGETYAAFEPALDYVVVKIPRWPFDKFPEANRTLGTEMKSVGEVMAIGRTFEEALQKAIRSLEI
GEPGLGPSPEELEADPEEIRRKIETPNDRRIFCIYAALKRGLMSVEEISELSGIDPWFVEKVKRIVEMEHEIVRRKDELL
EFIRTGEADEETVEFVREVKRTGFSDEQIAELLGVDEDEIREARLGVGVEATYKLVDTCAAEFAAVSPYFYSTYEEECEA
LRYD

Sequences:

>Translated_564_residues
MPETPNKVLIIGSGPIIVGQAAEFDYSGSQACKALREEGVEVVLVNSNPATIMTDPNMADRVYLEPLDARIVAKIIEEER
PDGILPTLGGQTGLNIAVELDEMGVLEEYDVEVLGTSVETIVRAEDRDEFRAFMKKIGEPVCASEAVSSVEEAKEVAEEI
GYPVVVRPAYTLGGTGGGIAEDEEELKRVVERGLEYSRVNQVLIEEYVGGWAEIEYEVMRDGSGNCITVCSMENVDPMGV
HTGESIVVAPAQTLTEEEHQMLRSAALHIIDALGVEGGCNIQFALHRETGEYRVIEVNPRVSRSSALASKATGYPIARIA
AKIAIGLRLDEIENQVTGETYAAFEPALDYVVVKIPRWPFDKFPEANRTLGTEMKSVGEVMAIGRTFEEALQKAIRSLEI
GEPGLGPSPEELEADPEEIRRKIETPNDRRIFCIYAALKRGLMSVEEISELSGIDPWFVEKVKRIVEMEHEIVRRKDELL
EFIRTGEADEETVEFVREVKRTGFSDEQIAELLGVDEDEIREARLGVGVEATYKLVDTCAAEFAAVSPYFYSTYEEECEA
LRYD
>Mature_563_residues
PETPNKVLIIGSGPIIVGQAAEFDYSGSQACKALREEGVEVVLVNSNPATIMTDPNMADRVYLEPLDARIVAKIIEEERP
DGILPTLGGQTGLNIAVELDEMGVLEEYDVEVLGTSVETIVRAEDRDEFRAFMKKIGEPVCASEAVSSVEEAKEVAEEIG
YPVVVRPAYTLGGTGGGIAEDEEELKRVVERGLEYSRVNQVLIEEYVGGWAEIEYEVMRDGSGNCITVCSMENVDPMGVH
TGESIVVAPAQTLTEEEHQMLRSAALHIIDALGVEGGCNIQFALHRETGEYRVIEVNPRVSRSSALASKATGYPIARIAA
KIAIGLRLDEIENQVTGETYAAFEPALDYVVVKIPRWPFDKFPEANRTLGTEMKSVGEVMAIGRTFEEALQKAIRSLEIG
EPGLGPSPEELEADPEEIRRKIETPNDRRIFCIYAALKRGLMSVEEISELSGIDPWFVEKVKRIVEMEHEIVRRKDELLE
FIRTGEADEETVEFVREVKRTGFSDEQIAELLGVDEDEIREARLGVGVEATYKLVDTCAAEFAAVSPYFYSTYEEECEAL
RYD

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0458

COG function: function code EF; Carbamoylphosphate synthase large subunit (split gene in MJ)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Homo sapiens, GI169790915, Length=564, Percent_Identity=48.4042553191489, Blast_Score=525, Evalue=1e-149,
Organism=Homo sapiens, GI21361331, Length=564, Percent_Identity=48.4042553191489, Blast_Score=525, Evalue=1e-149,
Organism=Homo sapiens, GI18105007, Length=559, Percent_Identity=48.8372093023256, Blast_Score=518, Evalue=1e-147,
Organism=Homo sapiens, GI170295797, Length=535, Percent_Identity=47.1028037383178, Blast_Score=483, Evalue=1e-136,
Organism=Escherichia coli, GI1786216, Length=569, Percent_Identity=52.8998242530756, Blast_Score=580, Evalue=1e-166,
Organism=Caenorhabditis elegans, GI193204318, Length=549, Percent_Identity=48.2695810564663, Blast_Score=530, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6322569, Length=556, Percent_Identity=49.4604316546763, Blast_Score=522, Evalue=1e-149,
Organism=Saccharomyces cerevisiae, GI6322331, Length=558, Percent_Identity=46.9534050179211, Blast_Score=511, Evalue=1e-146,
Organism=Saccharomyces cerevisiae, GI6319685, Length=402, Percent_Identity=24.6268656716418, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24642586, Length=554, Percent_Identity=49.0974729241877, Blast_Score=543, Evalue=1e-154,
Organism=Drosophila melanogaster, GI45555749, Length=554, Percent_Identity=49.0974729241877, Blast_Score=543, Evalue=1e-154,

Paralogues:

None

Copy number: 4701 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CARB1_METKA (Q8TWX0)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614194.1
- ProteinModelPortal:   Q8TWX0
- SMR:   Q8TWX0
- GeneID:   1477012
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0911
- NMPDR:   fig|190192.1.peg.907
- HOGENOM:   HBG405439
- OMA:   EPSAGEF
- BioCyc:   MKAN190192:MK0911-MONOMER
- BRENDA:   6.3.5.5
- HAMAP:   MF_01210_A
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR005479
- InterPro:   IPR005483
- InterPro:   IPR005481
- InterPro:   IPR005480
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:1.10.1030.10
- Gene3D:   G3DSA:3.40.50.20
- PRINTS:   PR00098

Pfam domain/function: PF00289 CPSase_L_chain; PF02786 CPSase_L_D2; PF02787 CPSase_L_D3; SSF48108 CarbamoylP_synth_lsu_oligo; SSF52440 PreATP-grasp-like

EC number: =6.3.5.5

Molecular weight: Translated: 62400; Mature: 62269

Theoretical pI: Translated: 4.15; Mature: 4.15

Prosite motif: PS50975 ATP_GRASP; PS00866 CPSASE_1; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPETPNKVLIIGSGPIIVGQAAEFDYSGSQACKALREEGVEVVLVNSNPATIMTDPNMAD
CCCCCCEEEEEECCCEEEECCCCCCCCHHHHHHHHHHCCCEEEEECCCCCEEEECCCCCC
RVYLEPLDARIVAKIIEEERPDGILPTLGGQTGLNIAVELDEMGVLEEYDVEVLGTSVET
EEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCHHCCHHEECCCHHH
IVRAEDRDEFRAFMKKIGEPVCASEAVSSVEEAKEVAEEIGYPVVVRPAYTLGGTGGGIA
HHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEECCCCCCCC
EDEEELKRVVERGLEYSRVNQVLIEEYVGGWAEIEYEVMRDGSGNCITVCSMENVDPMGV
CCHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHEEEEEEEECCCCCEEEEEECCCCCCCCC
HTGESIVVAPAQTLTEEEHQMLRSAALHIIDALGVEGGCNIQFALHRETGEYRVIEVNPR
CCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEEECCC
VSRSSALASKATGYPIARIAAKIAIGLRLDEIENQVTGETYAAFEPALDYVVVKIPRWPF
CCHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHCCCEEEEEECCCCC
DKFPEANRTLGTEMKSVGEVMAIGRTFEEALQKAIRSLEIGEPGLGPSPEELEADPEEIR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCHHHHH
RKIETPNDRRIFCIYAALKRGLMSVEEISELSGIDPWFVEKVKRIVEMEHEIVRRKDELL
HHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EFIRTGEADEETVEFVREVKRTGFSDEQIAELLGVDEDEIREARLGVGVEATYKLVDTCA
HHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHH
AEFAAVSPYFYSTYEEECEALRYD
HHHHHCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PETPNKVLIIGSGPIIVGQAAEFDYSGSQACKALREEGVEVVLVNSNPATIMTDPNMAD
CCCCCEEEEEECCCEEEECCCCCCCCHHHHHHHHHHCCCEEEEECCCCCEEEECCCCCC
RVYLEPLDARIVAKIIEEERPDGILPTLGGQTGLNIAVELDEMGVLEEYDVEVLGTSVET
EEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCHHCCHHEECCCHHH
IVRAEDRDEFRAFMKKIGEPVCASEAVSSVEEAKEVAEEIGYPVVVRPAYTLGGTGGGIA
HHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEECCCCCCCC
EDEEELKRVVERGLEYSRVNQVLIEEYVGGWAEIEYEVMRDGSGNCITVCSMENVDPMGV
CCHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHEEEEEEEECCCCCEEEEEECCCCCCCCC
HTGESIVVAPAQTLTEEEHQMLRSAALHIIDALGVEGGCNIQFALHRETGEYRVIEVNPR
CCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEEECCC
VSRSSALASKATGYPIARIAAKIAIGLRLDEIENQVTGETYAAFEPALDYVVVKIPRWPF
CCHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHCCCEEEEEECCCCC
DKFPEANRTLGTEMKSVGEVMAIGRTFEEALQKAIRSLEIGEPGLGPSPEELEADPEEIR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCHHHHH
RKIETPNDRRIFCIYAALKRGLMSVEEISELSGIDPWFVEKVKRIVEMEHEIVRRKDELL
HHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EFIRTGEADEETVEFVREVKRTGFSDEQIAELLGVDEDEIREARLGVGVEATYKLVDTCA
HHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHH
AEFAAVSPYFYSTYEEECEALRYD
HHHHHCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014