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The map label for this gene is MetG

Identifier: 20094286

GI number: 20094286

Start: 806368

End: 808374

Strand: Reverse

Name: MetG

Synonym: MK0850

Alternate gene names: 20094286

Gene position: 808374-806368 (Counterclockwise)

Preceding gene: 20094287

Following gene: 20094284

Centisome position: 47.69

GC content: 52.02

Gene sequence:

>2007_bases
TTGGGGAAGGTACTCGTAACTACGGCCTTGGCGTACACCAACGGCCCACTCCACATCGGCCACGTTAGGAGCACGTACCT
CCCCGCCGATGTGTACACGAGGTTCCTCAAGATGCGTGGTATCGACGCTATACATATCGGCGGGACTGACAACCACGGGG
TCCCGATAGCGCTACAGGCCGAGCTCGAGGGGAAGGATCCCGAGGAGATCGTGGAAAAATACCACGAGATGATCAAAGAG
GACTTAGAGCGATTGAACATCCACTTCGACGAGTTCTCATGCACCTGCCGCGAGTTCAATCCTGACCACGTGGATATGAC
ACAATGGTTTTTCAAGAGGTTGTACGAAGCGGGGTACATAGAGGAGAGGGAGGTCGAACAACTCTACTGTCCTGAGTGCG
AAAGGCCCCTTCCGGACAGGTACGTCGAAGGAGTATGCCCCTACTGCGGTGCTGAAGGTGCCCGAGGCGACCATTGCGAG
GCCTGCGGTCGCTACCTGGAGCCCGTACAACTCGAAGAGCCGAGGTGTGTCATCTGTGGATCGAAGCCAGAGGTCCGGCG
CACTATGCACCTGTTCTTCAAGCTTAGCGAATTCGAAGAAGATCTGAAGAAGTGGTTAGAGTCCAACGACAATCTGCCTA
AGAACGTGCGTAATTATGCCATCCAATGGGTTCGTGAAGGACTCAAGGATTGGGATATAGTGCGTGATCTAGACTGGGGA
GTACCCGTTCCCCTGGAAGGATACGAAGATAAGGTGTTTTATGTTTGGTTCGATGCTCCTATAGGATACGTAACGTTCAC
GAAACAATACTGTGATAGGGTAGGTCAAGACTGGAAAGACTACTGGTTTAGTGAAGATACGAAAATAGTTCACTTCATCG
GTAAGGACATAATAGTCCATCACGCCCTGTTCTGGCCGGCCATGCTCATGGGCGTCGGAGCGACTCTCCCATACACTATC
GTGGCTGGCGAGTATTTGACCTTAGAAGGAGAAAAGATGAGTACAAGTCGTGGTTGGGTAGTGTGGGTCAAAGACTTCAC
CAAGCTGTTCCCTGCGGATCTGCTACGATACTACCTCATAGTGGTGAGCCCGCTCACCAGGGATGCGGATTTCTCGTGGG
GAGACTTCCGGGACCGTGTGAACAACGAATTAGTGGCTAACTTAGGTAACTTTGTGTATCGCACGCTATCGTTTATTTAT
AGATTCTTAGACGGAAACGTTCCCGAAGCCGAGACTGACCAGGAGATAGTCGATAAGATAAAAGAAACTCACCAGCGTGT
GACGGAACATCTGGAGAAGTTCCGATTCAGGGAGGCGCTCACAGAGGTATTACGTCTATCTAAGTTTGGGAATGAGTACT
TCCAAGAACACGAGCCGTGGAAACTTAAGGATGAAGATCCAGAGCGTTGTGCCGAAGTTCTACGTGGGTGCGCTAGAATT
GTGAAAGCGCTAGCTGTAATGTTAGCCCCGTTCCTTCCGGATAGTGCGGAGAAGATTTGGCAGAGTCTTGGTTACGAAGA
CTCCGTACATGACGTCGATTGGGAAGAAGCCTTGGAGGACGTAGAGACTAAGGAGATACCGGAGCCCGAGCCCATTTTCC
CGAAGGTAACGGAGGAGGACCTTGAGAAAGCTAAGGCGTTACTGCCGGAAGAATCAGGAGAGTCCGAAGGGCAGGACGAC
GAGTACGTATCGCTCGAGGAATTCAATCGATTAGACCTGCGGGTAGGCAAGATCAAAGAAGCGGAACGTGTCGAGGGCTC
TGATCGACTCATCAAGCTCCGGATCGATATCGGAGATCGTACCGTAACAGCAGTAGCCGGACTGTATCCTACGTATGAGC
CAGAGGAGCTCGTCGGACGTAAGGTGGTTGTACTAGCCAACATCCAACCCAAAGAGATGTTCGGCGTACGGTCGGAAGCG
ATGATCCTGGCCGTTGGGGACGAACCCGCATTGTTGACTATCGACGAGAGTAAGAGGGAAGTGGAGCCCGGAGAGCGGAT
CAGATGA

Upstream 100 bases:

>100_bases
CGTACCTTCTATGGTGAAGCAGCTGAGGCGCCACGGGGTGGTCGTGTCCCGGATAGTGACGTTCACGGACGTAGCCAGGT
GCATTTGGGGGTAGCCGGTA

Downstream 100 bases:

>100_bases
TTCACTCACCAAGCGTCCTGCGGAGCCCCAGCTGCTTGAGCAGGCCGATCACAGGGTCCGGGGTAGGATGCACCTCGATC
AGCCGCCAGTCAGGTTCCAC

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 668; Mature: 667

Protein sequence:

>668_residues
MGKVLVTTALAYTNGPLHIGHVRSTYLPADVYTRFLKMRGIDAIHIGGTDNHGVPIALQAELEGKDPEEIVEKYHEMIKE
DLERLNIHFDEFSCTCREFNPDHVDMTQWFFKRLYEAGYIEEREVEQLYCPECERPLPDRYVEGVCPYCGAEGARGDHCE
ACGRYLEPVQLEEPRCVICGSKPEVRRTMHLFFKLSEFEEDLKKWLESNDNLPKNVRNYAIQWVREGLKDWDIVRDLDWG
VPVPLEGYEDKVFYVWFDAPIGYVTFTKQYCDRVGQDWKDYWFSEDTKIVHFIGKDIIVHHALFWPAMLMGVGATLPYTI
VAGEYLTLEGEKMSTSRGWVVWVKDFTKLFPADLLRYYLIVVSPLTRDADFSWGDFRDRVNNELVANLGNFVYRTLSFIY
RFLDGNVPEAETDQEIVDKIKETHQRVTEHLEKFRFREALTEVLRLSKFGNEYFQEHEPWKLKDEDPERCAEVLRGCARI
VKALAVMLAPFLPDSAEKIWQSLGYEDSVHDVDWEEALEDVETKEIPEPEPIFPKVTEEDLEKAKALLPEESGESEGQDD
EYVSLEEFNRLDLRVGKIKEAERVEGSDRLIKLRIDIGDRTVTAVAGLYPTYEPEELVGRKVVVLANIQPKEMFGVRSEA
MILAVGDEPALLTIDESKREVEPGERIR

Sequences:

>Translated_668_residues
MGKVLVTTALAYTNGPLHIGHVRSTYLPADVYTRFLKMRGIDAIHIGGTDNHGVPIALQAELEGKDPEEIVEKYHEMIKE
DLERLNIHFDEFSCTCREFNPDHVDMTQWFFKRLYEAGYIEEREVEQLYCPECERPLPDRYVEGVCPYCGAEGARGDHCE
ACGRYLEPVQLEEPRCVICGSKPEVRRTMHLFFKLSEFEEDLKKWLESNDNLPKNVRNYAIQWVREGLKDWDIVRDLDWG
VPVPLEGYEDKVFYVWFDAPIGYVTFTKQYCDRVGQDWKDYWFSEDTKIVHFIGKDIIVHHALFWPAMLMGVGATLPYTI
VAGEYLTLEGEKMSTSRGWVVWVKDFTKLFPADLLRYYLIVVSPLTRDADFSWGDFRDRVNNELVANLGNFVYRTLSFIY
RFLDGNVPEAETDQEIVDKIKETHQRVTEHLEKFRFREALTEVLRLSKFGNEYFQEHEPWKLKDEDPERCAEVLRGCARI
VKALAVMLAPFLPDSAEKIWQSLGYEDSVHDVDWEEALEDVETKEIPEPEPIFPKVTEEDLEKAKALLPEESGESEGQDD
EYVSLEEFNRLDLRVGKIKEAERVEGSDRLIKLRIDIGDRTVTAVAGLYPTYEPEELVGRKVVVLANIQPKEMFGVRSEA
MILAVGDEPALLTIDESKREVEPGERIR
>Mature_667_residues
GKVLVTTALAYTNGPLHIGHVRSTYLPADVYTRFLKMRGIDAIHIGGTDNHGVPIALQAELEGKDPEEIVEKYHEMIKED
LERLNIHFDEFSCTCREFNPDHVDMTQWFFKRLYEAGYIEEREVEQLYCPECERPLPDRYVEGVCPYCGAEGARGDHCEA
CGRYLEPVQLEEPRCVICGSKPEVRRTMHLFFKLSEFEEDLKKWLESNDNLPKNVRNYAIQWVREGLKDWDIVRDLDWGV
PVPLEGYEDKVFYVWFDAPIGYVTFTKQYCDRVGQDWKDYWFSEDTKIVHFIGKDIIVHHALFWPAMLMGVGATLPYTIV
AGEYLTLEGEKMSTSRGWVVWVKDFTKLFPADLLRYYLIVVSPLTRDADFSWGDFRDRVNNELVANLGNFVYRTLSFIYR
FLDGNVPEAETDQEIVDKIKETHQRVTEHLEKFRFREALTEVLRLSKFGNEYFQEHEPWKLKDEDPERCAEVLRGCARIV
KALAVMLAPFLPDSAEKIWQSLGYEDSVHDVDWEEALEDVETKEIPEPEPIFPKVTEEDLEKAKALLPEESGESEGQDDE
YVSLEEFNRLDLRVGKIKEAERVEGSDRLIKLRIDIGDRTVTAVAGLYPTYEPEELVGRKVVVLANIQPKEMFGVRSEAM
ILAVGDEPALLTIDESKREVEPGERIR

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI14043022, Length=560, Percent_Identity=37.6785714285714, Blast_Score=370, Evalue=1e-102,
Organism=Homo sapiens, GI24308436, Length=535, Percent_Identity=27.6635514018692, Blast_Score=177, Evalue=3e-44,
Organism=Homo sapiens, GI7661872, Length=125, Percent_Identity=34.4, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1788432, Length=688, Percent_Identity=32.8488372093023, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI1789455, Length=97, Percent_Identity=44.3298969072165, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17540794, Length=519, Percent_Identity=36.0308285163777, Blast_Score=350, Evalue=2e-96,
Organism=Caenorhabditis elegans, GI212645096, Length=522, Percent_Identity=26.6283524904215, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI71992781, Length=331, Percent_Identity=27.7945619335347, Blast_Score=116, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI71997510, Length=133, Percent_Identity=33.8345864661654, Blast_Score=69, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71997517, Length=133, Percent_Identity=33.8345864661654, Blast_Score=69, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321704, Length=558, Percent_Identity=34.4086021505376, Blast_Score=331, Evalue=3e-91,
Organism=Saccharomyces cerevisiae, GI6321610, Length=537, Percent_Identity=26.0707635009311, Blast_Score=136, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6323414, Length=229, Percent_Identity=24.8908296943231, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24655311, Length=596, Percent_Identity=35.2348993288591, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI21358483, Length=526, Percent_Identity=23.574144486692, Blast_Score=132, Evalue=8e-31,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_METKA (Q8TX28)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614133.1
- ProteinModelPortal:   Q8TX28
- GeneID:   1476951
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0850
- NMPDR:   fig|190192.1.peg.846
- HOGENOM:   HBG721667
- OMA:   SAYQPEQ
- BioCyc:   MKAN190192:MK0850-MONOMER
- BRENDA:   6.1.1.10
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 77387; Mature: 77256

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD

Important sites: BINDING 335-335

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKVLVTTALAYTNGPLHIGHVRSTYLPADVYTRFLKMRGIDAIHIGGTDNHGVPIALQA
CCCEEEEEEHHHCCCCEEECCCHHHCCCHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEE
ELEGKDPEEIVEKYHEMIKEDLERLNIHFDEFSCTCREFNPDHVDMTQWFFKRLYEAGYI
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHCCCC
EEREVEQLYCPECERPLPDRYVEGVCPYCGAEGARGDHCEACGRYLEPVQLEEPRCVICG
CHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCEEEEC
SKPEVRRTMHLFFKLSEFEEDLKKWLESNDNLPKNVRNYAIQWVREGLKDWDIVRDLDWG
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
VPVPLEGYEDKVFYVWFDAPIGYVTFTKQYCDRVGQDWKDYWFSEDTKIVHFIGKDIIVH
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHCCCCCEEEEHHHHHHHHH
HALFWPAMLMGVGATLPYTIVAGEYLTLEGEKMSTSRGWVVWVKDFTKLFPADLLRYYLI
HHHHHHHHHHHCCCCCCEEEEECCEEEEECCEECCCCCEEEEHHHHHHHHHHHHHHHHHH
VVSPLTRDADFSWGDFRDRVNNELVANLGNFVYRTLSFIYRFLDGNVPEAETDQEIVDKI
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
KETHQRVTEHLEKFRFREALTEVLRLSKFGNEYFQEHEPWKLKDEDPERCAEVLRGCARI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
VKALAVMLAPFLPDSAEKIWQSLGYEDSVHDVDWEEALEDVETKEIPEPEPIFPKVTEED
HHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHH
LEKAKALLPEESGESEGQDDEYVSLEEFNRLDLRVGKIKEAERVEGSDRLIKLRIDIGDR
HHHHHHHCCCCCCCCCCCCCCEEEHHHHCCHHHHHCCCCHHHHCCCCCCEEEEEEECCCC
TVTAVAGLYPTYEPEELVGRKVVVLANIQPKEMFGVRSEAMILAVGDEPALLTIDESKRE
HHHHHHHCCCCCCHHHHCCCEEEEEECCCHHHHHCCCCCEEEEEECCCCEEEEECCCCCC
VEPGERIR
CCCCCCCC
>Mature Secondary Structure 
GKVLVTTALAYTNGPLHIGHVRSTYLPADVYTRFLKMRGIDAIHIGGTDNHGVPIALQA
CCEEEEEEHHHCCCCEEECCCHHHCCCHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEE
ELEGKDPEEIVEKYHEMIKEDLERLNIHFDEFSCTCREFNPDHVDMTQWFFKRLYEAGYI
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHCCCC
EEREVEQLYCPECERPLPDRYVEGVCPYCGAEGARGDHCEACGRYLEPVQLEEPRCVICG
CHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCEEEEC
SKPEVRRTMHLFFKLSEFEEDLKKWLESNDNLPKNVRNYAIQWVREGLKDWDIVRDLDWG
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
VPVPLEGYEDKVFYVWFDAPIGYVTFTKQYCDRVGQDWKDYWFSEDTKIVHFIGKDIIVH
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHCCCCCEEEEHHHHHHHHH
HALFWPAMLMGVGATLPYTIVAGEYLTLEGEKMSTSRGWVVWVKDFTKLFPADLLRYYLI
HHHHHHHHHHHCCCCCCEEEEECCEEEEECCEECCCCCEEEEHHHHHHHHHHHHHHHHHH
VVSPLTRDADFSWGDFRDRVNNELVANLGNFVYRTLSFIYRFLDGNVPEAETDQEIVDKI
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
KETHQRVTEHLEKFRFREALTEVLRLSKFGNEYFQEHEPWKLKDEDPERCAEVLRGCARI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
VKALAVMLAPFLPDSAEKIWQSLGYEDSVHDVDWEEALEDVETKEIPEPEPIFPKVTEED
HHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHH
LEKAKALLPEESGESEGQDDEYVSLEEFNRLDLRVGKIKEAERVEGSDRLIKLRIDIGDR
HHHHHHHCCCCCCCCCCCCCCEEEHHHHCCHHHHHCCCCHHHHCCCCCCEEEEEEECCCC
TVTAVAGLYPTYEPEELVGRKVVVLANIQPKEMFGVRSEAMILAVGDEPALLTIDESKRE
HHHHHHHCCCCCCHHHHCCCEEEEEECCCHHHHHCCCCCEEEEEECCCCEEEEECCCCCC
VEPGERIR
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014