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The map label for this gene is eRF1

Identifier: 20094196

GI number: 20094196

Start: 722643

End: 723872

Strand: Reverse

Name: eRF1

Synonym: MK0759

Alternate gene names: 20094196

Gene position: 723872-722643 (Counterclockwise)

Preceding gene: 20094199

Following gene: 20094195

Centisome position: 42.71

GC content: 56.5

Gene sequence:

>1230_bases
ATGGCCGAATCGTCTACGGTCGAGAGATATAGGTTCAGGAAGATGATAGAGAGACTGGAAAACCTGCGCGGACAAGGCAC
CGAGCTCATCACGATTTACATCCCGCCGGAGAATCGTCTGAGCGACGTCATCGCTCAGATGCGGGAGGAGTACTCCCAAG
CCAGCAACATCAAGAGTAAGCGCACCCGGAAGAACGTCCAATCAGCTATCGAGGTCGTGATGCAGCGTCTCAAAATGGTC
GGAGAGACTCCGGAAAACGGACTCGTAGTTCTCGTCGGCACCGTCCAGGACGGTACCAAGGAGAAGATGGTCGCGGAGCT
GATCGAACCGCCCGAGCCCGTGGACCGGTTCATCTACCGATGTGATTCGAAATTCTATCTAGAACCCCTCAAGGAATACC
TCGAAGAGAAGGACGTCTACGGAATCCTGGTAATGGACCGCCGGGAGGCCACGATAGGTCTCGTGAAAGGTAAGCGAATC
GAACCCGTGAAGAGGCTGACGTCCGACGTGCCGGGTAAGCATAAGGCCGGAGGTCAATCTCAGCGTAGGTTCGACCGGTT
GATCGAGCACGCCGCCCACGAGTTTTACCAGAAGGTGGGAGAAGCCGCCCGCGAGGCTTTCGAAGACGTGAAGGATCTGA
AGGGAATCATCGTCGGAGGTCCAGGTCCCACGAAGGAGGAGTTCCTGGACGGCGACTACCTCCCCAAGGATCTAAAGGAA
AAGGTACTCACCGTGGTGGACGTCGGCAACACGGACGAGTCCGGGCTCCGGGAGGCCCTGAACAAAGCCGAAGAAGCGCT
GAAGGAAGCCGAGCTAGTCCGGGAGAAGCGCCTCGTGCGAAAGTTCATGGAGGAGGCCGTGAACGGTGAACTCGCGGCGT
ATGGAGAGGAAGTCGACGAACTCCTCAAGATGGGTGCCGTGGAGGTACTGCTAGTTTCCGAGGACCTCGAGGGATACAAG
GTAATCCTACGATGTCCGGAGTGCGGGTACGAGAACATCGTTACGGTGAAGGAGAAAGACGAGGCGAAGAAATACGTGGA
AGAGTGCCCGGAATGCGGGGAAGCAGAGCTCAACGTGGAAGAAATCAAAGACATAGTGGATTACTACGTGGAACTGGCCG
AGCAGATGGGATCCAACGTCGAGATCATCTCAACCGAGACCGAAGAGGGCGCTCAGTTTTATAACGCGTTTCGGGGACTC
GGAGCCCTGCTCAGGTTCCGACCCAAGTGA

Upstream 100 bases:

>100_bases
GGCGGTTTCCTTGATGCGTTCGGGTTTATCCACGTTTACGACCGAGCCACCCAGTGCCACGACCGAATACATGCTGTCTC
ACCCCTAGGGGGTGTTCACG

Downstream 100 bases:

>100_bases
AGGGGTTCGCCCACGTTGGCCGAGGAAACCACACCTCGCGACCCGTTTTCGGTTACGATTCGCACCATAAGAACATCGAT
CCGGGACGCGATCTCGTCCG

Product: peptide chain release factor 1

Products: NA

Alternate protein names: Translation termination factor aRF1

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MAESSTVERYRFRKMIERLENLRGQGTELITIYIPPENRLSDVIAQMREEYSQASNIKSKRTRKNVQSAIEVVMQRLKMV
GETPENGLVVLVGTVQDGTKEKMVAELIEPPEPVDRFIYRCDSKFYLEPLKEYLEEKDVYGILVMDRREATIGLVKGKRI
EPVKRLTSDVPGKHKAGGQSQRRFDRLIEHAAHEFYQKVGEAAREAFEDVKDLKGIIVGGPGPTKEEFLDGDYLPKDLKE
KVLTVVDVGNTDESGLREALNKAEEALKEAELVREKRLVRKFMEEAVNGELAAYGEEVDELLKMGAVEVLLVSEDLEGYK
VILRCPECGYENIVTVKEKDEAKKYVEECPECGEAELNVEEIKDIVDYYVELAEQMGSNVEIISTETEEGAQFYNAFRGL
GALLRFRPK

Sequences:

>Translated_409_residues
MAESSTVERYRFRKMIERLENLRGQGTELITIYIPPENRLSDVIAQMREEYSQASNIKSKRTRKNVQSAIEVVMQRLKMV
GETPENGLVVLVGTVQDGTKEKMVAELIEPPEPVDRFIYRCDSKFYLEPLKEYLEEKDVYGILVMDRREATIGLVKGKRI
EPVKRLTSDVPGKHKAGGQSQRRFDRLIEHAAHEFYQKVGEAAREAFEDVKDLKGIIVGGPGPTKEEFLDGDYLPKDLKE
KVLTVVDVGNTDESGLREALNKAEEALKEAELVREKRLVRKFMEEAVNGELAAYGEEVDELLKMGAVEVLLVSEDLEGYK
VILRCPECGYENIVTVKEKDEAKKYVEECPECGEAELNVEEIKDIVDYYVELAEQMGSNVEIISTETEEGAQFYNAFRGL
GALLRFRPK
>Mature_408_residues
AESSTVERYRFRKMIERLENLRGQGTELITIYIPPENRLSDVIAQMREEYSQASNIKSKRTRKNVQSAIEVVMQRLKMVG
ETPENGLVVLVGTVQDGTKEKMVAELIEPPEPVDRFIYRCDSKFYLEPLKEYLEEKDVYGILVMDRREATIGLVKGKRIE
PVKRLTSDVPGKHKAGGQSQRRFDRLIEHAAHEFYQKVGEAAREAFEDVKDLKGIIVGGPGPTKEEFLDGDYLPKDLKEK
VLTVVDVGNTDESGLREALNKAEEALKEAELVREKRLVRKFMEEAVNGELAAYGEEVDELLKMGAVEVLLVSEDLEGYKV
ILRCPECGYENIVTVKEKDEAKKYVEECPECGEAELNVEEIKDIVDYYVELAEQMGSNVEIISTETEEGAQFYNAFRGLG
ALLRFRPK

Specific function: Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA

COG id: COG1503

COG function: function code J; Peptide chain release factor 1 (eRF1)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic release factor 1 family

Homologues:

Organism=Homo sapiens, GI4759034, Length=412, Percent_Identity=33.7378640776699, Blast_Score=240, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI72000368, Length=408, Percent_Identity=31.6176470588235, Blast_Score=219, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6319619, Length=409, Percent_Identity=33.7408312958435, Blast_Score=227, Evalue=2e-60,
Organism=Drosophila melanogaster, GI28574632, Length=410, Percent_Identity=32.6829268292683, Blast_Score=227, Evalue=9e-60,
Organism=Drosophila melanogaster, GI21356433, Length=410, Percent_Identity=32.6829268292683, Blast_Score=227, Evalue=9e-60,
Organism=Drosophila melanogaster, GI24667392, Length=410, Percent_Identity=32.6829268292683, Blast_Score=227, Evalue=9e-60,
Organism=Drosophila melanogaster, GI24667388, Length=410, Percent_Identity=32.6829268292683, Blast_Score=227, Evalue=9e-60,
Organism=Drosophila melanogaster, GI24667380, Length=410, Percent_Identity=32.6829268292683, Blast_Score=227, Evalue=9e-60,
Organism=Drosophila melanogaster, GI24667384, Length=410, Percent_Identity=32.6829268292683, Blast_Score=227, Evalue=9e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF1_METKA (Q8TXB5)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614043.1
- ProteinModelPortal:   Q8TXB5
- SMR:   Q8TXB5
- GeneID:   1476860
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0759
- NMPDR:   fig|190192.1.peg.756
- HOGENOM:   HBG497188
- OMA:   GDYLHHE
- ProtClustDB:   PRK04011
- BioCyc:   MKAN190192:MK0759-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00424
- InterPro:   IPR005140
- InterPro:   IPR005141
- InterPro:   IPR005142
- InterPro:   IPR020918
- InterPro:   IPR004403
- PANTHER:   PTHR10113
- PANTHER:   PTHR10113:SF2
- TIGRFAMs:   TIGR03676

Pfam domain/function: PF03463 eRF1_1; PF03464 eRF1_2; PF03465 eRF1_3

EC number: NA

Molecular weight: Translated: 46486; Mature: 46354

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAESSTVERYRFRKMIERLENLRGQGTELITIYIPPENRLSDVIAQMREEYSQASNIKSK
CCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCHHHH
RTRKNVQSAIEVVMQRLKMVGETPENGLVVLVGTVQDGTKEKMVAELIEPPEPVDRFIYR
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHH
CDSKFYLEPLKEYLEEKDVYGILVMDRREATIGLVKGKRIEPVKRLTSDVPGKHKAGGQS
HCCHHHHHHHHHHHHHCCCEEEEEEECCCHHEEHHCCCCCCHHHHHHHCCCCCCCCCCHH
QRRFDRLIEHAAHEFYQKVGEAAREAFEDVKDLKGIIVGGPGPTKEEFLDGDYLPKDLKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHCCCCCCCHHHHH
KVLTVVDVGNTDESGLREALNKAEEALKEAELVREKRLVRKFMEEAVNGELAAYGEEVDE
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
LLKMGAVEVLLVSEDLEGYKVILRCPECGYENIVTVKEKDEAKKYVEECPECGEAELNVE
HHHCCCCEEEEEECCCCCCEEEEECCCCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCHH
EIKDIVDYYVELAEQMGSNVEIISTETEEGAQFYNAFRGLGALLRFRPK
HHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AESSTVERYRFRKMIERLENLRGQGTELITIYIPPENRLSDVIAQMREEYSQASNIKSK
CCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCHHHH
RTRKNVQSAIEVVMQRLKMVGETPENGLVVLVGTVQDGTKEKMVAELIEPPEPVDRFIYR
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHH
CDSKFYLEPLKEYLEEKDVYGILVMDRREATIGLVKGKRIEPVKRLTSDVPGKHKAGGQS
HCCHHHHHHHHHHHHHCCCEEEEEEECCCHHEEHHCCCCCCHHHHHHHCCCCCCCCCCHH
QRRFDRLIEHAAHEFYQKVGEAAREAFEDVKDLKGIIVGGPGPTKEEFLDGDYLPKDLKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHCCCCCCCHHHHH
KVLTVVDVGNTDESGLREALNKAEEALKEAELVREKRLVRKFMEEAVNGELAAYGEEVDE
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
LLKMGAVEVLLVSEDLEGYKVILRCPECGYENIVTVKEKDEAKKYVEECPECGEAELNVE
HHHCCCCEEEEEECCCCCCEEEEECCCCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCHH
EIKDIVDYYVELAEQMGSNVEIISTETEEGAQFYNAFRGLGALLRFRPK
HHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014